scholarly journals PRESERVATION OF AZOTOBACTER ON CUCUMIS SATIVUS L. SEEDS UNDER DIFFERENT TEMPERATURES

2018 ◽  
Vol 27 ◽  
pp. 11-17
Author(s):  
O. M. Bilokonska ◽  
S. F. Kozar ◽  
T. A. Yevtushenko ◽  
T. O. Usmanova

The results of studies on the effect of temperature on the preservation of Azotobacter chroococcumM-70 and Azotobacter vinelandii M-X cells on the seeds of Cucumis sativus L. are presented. In threemonths, the number of bacterial cells on the seeds, which were kept at + 4°C, was significantly higherthan the parameters of the storage of seeds at +28° С. Azotobacter cysts were preserved better thanvegetative cells. To increase the viability of bacterial cells on the seeds, it is proposed to use a complex ofchemical substances that provides better survival of both vegetative cells and Azotobacter cysts. Theresults of the studies can be used to develop new ways to improve the viability of useful soilmicroorganisms on seeds of agricultural crops

2010 ◽  
Vol 73 (5) ◽  
pp. 839-848 ◽  
Author(s):  
M. C. KANDHAI ◽  
M. W. REIJ ◽  
M. van SCHOTHORST ◽  
L. G. M. GORRIS ◽  
M. H. ZWIETERING

The aim of this study was to determine the survival of two strains of Cronobacter (Enterobacter sakazakii) and six other bacterial strains inoculated into dry powdered infant formula (PIF) stored for 22 weeks at several temperatures between 7 and 42°C. The experimental setup involved a relatively high initial concentration of bacteria, around 104 CFU/g of powder, and enumeration of survivors with a minimum detection level of 100 CFU/g. For all strains tested, it was found that the number of bacterial cells decreased faster with increasing temperature. Cronobacter spp. cells generally survived better at high temperatures (37 and 42°C) than the other bacteria, while such a difference in survival was not apparent at other temperatures. To describe the effect of temperature on survival, both the Weibull distribution model and the log-linear model were tested. At 22°C, decline rates of 0.011 and 0.008 log units per day were found for Cronobacter sakazakii ATCC 29544 and Cronobacter strain MC10, respectively. Assuming a linear relationship between log-transformed D-values and temperature, z-values estimated for C. sakazakii ATCC 29544 and Cronobacter MC10 were 13.3 and 23.5°C, respectively. Such differences found in resistance among Cronobacter spp. would be relevant to consider when establishing quantitative risk assessments on consumer risks related to PIF.


Author(s):  
D. T. Gauld ◽  
J. E. G. Raymont

The respiratory rates of three species of planktonic copepods, Acartia clausi, Centropages hamatus and Temora longicornis, were measured at four different temperatures.The relationship between respiratory rate and temperature was found to be similar to that previously found for Calanus, although the slope of the curves differed in the different species.The observations on Centropages at 13 and 170 C. can be divided into two groups and it is suggested that the differences are due to the use of copepods from two different generations.The relationship between the respiratory rates and lengths of Acartia and Centropages agreed very well with that previously found for other species. That for Temora was rather different: the difference is probably due to the distinct difference in the shape of the body of Temora from those of the other species.The application of these measurements to estimates of the food requirements of the copepods is discussed.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aiyan Guan ◽  
Inge Van Damme ◽  
Frank Devlieghere ◽  
Sarah Gabriël

AbstractAnisakidae, marine nematodes, are underrecognized fish-borne zoonotic parasites. Studies on factors that could trigger parasites to actively migrate out of the fish are very limited. The objective of this study was to assess the impact of different environmental conditions (temperature, CO2 and O2) on larval motility (in situ movement) and mobility (migration) in vitro. Larvae were collected by candling or enzymatic digestion from infected fish, identified morphologically and confirmed molecularly. Individual larvae were transferred to a semi-solid Phosphate Buffered Saline agar, and subjected to different temperatures (6 ℃, 12 ℃, 22 ℃, 37 ℃) at air conditions. Moreover, different combinations of CO2 and O2 with N2 as filler were tested, at both 6 °C and 12 °C. Video recordings of larvae were translated into scores for larval motility and mobility. Results showed that temperature had significant influence on larval movements, with the highest motility and mobility observed at 22 ℃ for Anisakis spp. larvae and 37 ℃ for Pseudoterranova spp. larvae. During the first 10 min, the median migration of Anisakis spp. larvae was 10 cm at 22 ℃, and the median migration of Pseudoterranova spp. larvae was 3 cm at 37 ℃. Larval mobility was not significantly different under the different CO2 or O2 conditions at 6 °C and 12 ℃. It was concluded that temperature significantly facilitated larval movement with the optimum temperature being different for Anisakis spp. and Pseudoterranova spp., while CO2 and O2 did not on the short term. This should be further validated in parasite-infected/spiked fish fillets.


Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 134
Author(s):  
Ana Isabel Galván ◽  
Alicia Rodríguez ◽  
Alberto Martín ◽  
Manuel Joaquín Serradilla ◽  
Ana Martínez-Dorado ◽  
...  

Dried fig is susceptible to infection by Aspergillus flavus, the major producer of the carcinogenic mycotoxins. This fruit may be contaminated by the fungus throughout the entire chain production, especially during natural sun-drying, post-harvest, industrial processing, storage, and fruit retailing. Correct management of such critical stages is necessary to prevent mould growth and mycotoxin accumulation, with temperature being one of the main factors associated with these problems. The effect of different temperatures (5, 16, 25, 30, and 37 °C) related to dried-fig processing on growth, one of the regulatory genes of aflatoxin pathway (aflR) and mycotoxin production by A. flavus, was assessed. Firstly, growth and aflatoxin production of 11 A. flavus strains were checked before selecting two strains (M30 and M144) for in-depth studies. Findings showed that there were enormous differences in aflatoxin amounts and related-gene expression between the two selected strains. Based on the results, mild temperatures, and changes in temperature during drying and storage of dried figs should be avoided. Drying should be conducted at temperatures >30 °C and close to 37 °C, while industry processing, storage, and retailing of dried figs are advisable to perform at refrigeration temperatures (<10 °C) to avoid mycotoxin production.


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