scholarly journals Characterization of Exopolysaccharides Produced by Marine Bacillus Megaterium

2019 ◽  
Vol 8 (3S2) ◽  
pp. 287-290
Keyword(s):  
Molecular Weight ◽  
16S Rrna ◽  
Bacillus Megaterium ◽  
Bacterial Isolate ◽  
Synthetic Polymers ◽  
Chemical Compositions ◽  
Bacterial Isolates ◽  
Large Molecular Weight

Exopolysaccharides (EPSs) are polymers with large molecular weight that consist of various residues of sugar. These are desired because the substitution of synthetic polymers is degradable and non-toxic. Most microorganisms have the ability to synthesize and excrete exo polysaccharides with new chemical compositions, characteristics and structures in order to have important applications in various areas. The current study based on marine bacterial isolates screening the production of exo polysaccharide. Among the four exopolysaccharide producing isolates, the isolate PMSS12 had the highest production of exo polysaccharides. The efficient marine bacterial isolate PMSS12 was further identified by sequence of 16S rRNA. The PMSS12 isolate was confirmed as Bacillus megaterium. Then the exopolysaccharide produced by Bacillus megaterium was characterized by using FTIR, NMR and SEM.

scholarly journals Hydrogel: Preparation, Characterization and Applications

2020 ◽  
Vol 3 (01) ◽  
Author(s):  
Ujjwal Nautiyal ◽  
Nandini Sahu ◽  
Diksha Gupta
Keyword(s):  
Drug Delivery ◽  
Molecular Weight ◽  
Biological Tissue ◽  
Biological Fluids ◽  
Three Dimensional ◽  
Review Article ◽  
Advantages And Disadvantages ◽  
Concise Review ◽  
Large Molecular Weight

This review article is intended to provide an overview of hydrogel as novel vesicular drug delivery system.For the treatment of many diseases large molecular weight proteins are required. These can be available with the availability of Hydrogels. Hydrogels are hydrophilic, three-dimensional networks, which are able to imbibe large amounts of water or biological fluids, and thus resemble, to a large extent, a biological tissue. They are insoluble due to the presence of chemical (tie-points, junctions) and/or physical crosslinks such as entanglements and crystallites. It has focused on to present a concise review on the applications of hydrogels in the pharmaceutical field, hydrogel properties, method of preparation of hydrogel, advantages and disadvantages of hydrogel, characterization of hydrogel.

scholarly journals Penggunaan Bakteri Kitinolitik sebagai Pengendali Hayati Colletotrichum capsici pada Tanaman Cabai

2018 ◽  
Vol 14 (4) ◽  
pp. 120
Author(s):  
Dian Syahfitri ◽  
Nisa Rachmania Mubarik ◽  
Lisdar A Manaf
Keyword(s):  
Biocontrol Agent ◽  
Bacterial Isolate ◽  
Disease Incidence ◽  
Antagonistic Activity ◽  
Biocontrol Agents ◽  
Bacterial Isolates ◽  
Chitinolytic Bacteria ◽  
Colletotrichum Capsici ◽  
Carrier Materials

Use of Chitinolytic Bacteria as Biological Control of Colletotrichum capsici on Chili PlantsColletotrichum capsici is known as the causal agent of anthracnose disease in chili plant and may cause reduction of crop yield. Chitinolytic bacteria, namely Serratia marcescens KAHN 15.12, Bacillus thuringiensis SAHA 12.12, and BAE 36 were reported to have antagonistic activity against C. capsici. Therefore, a study was conducted to determine the potential of chitinolytic bacteria on controlling C. capsici on chili plants in greenhouse experiment. Three bacterial isolates used as biocontrol agent was formulated by using talcum as carrier materials. The methodologies consisted of characterization of bacterial isolates, formulation of biocontrol agent, viability test of bacterial isolate, efficacy of biocontrol agents in the laboratory and in the greenhouse.  Disease severity in the laboratory reached 64% when chili treated with isolate formulation of BAE 36.  In the greenhouse, BAE 36 isolate formulation and consortium formulation were able to suppress infection of C. capsici; each was indicated by disease incidence of 25% and 50%, respectively. These results indicated that chitinolytic bacterial formulations could be potencial as biocontrol agents of C. capsici.

Further characterization of the molecular species formed during the biosynthesis of rat luteinizing hormone

1982 ◽  
Vol 93 (2) ◽  
pp. 169-176 ◽  
Author(s):  
M. Chowdhury ◽  
H. E. Grotjan ◽  
E. Steinberger
Keyword(s):  
Molecular Weight ◽  
Luteinizing Hormone ◽  
Concanavalin A ◽  
Molecular Species ◽  
Apparent Molecular Weight ◽  
Large Molecular Weight ◽  
Molecular Weight Form

During the biosynthesis of rat LH (rLH) there are four molecular species found intracellularly: native rLH, a species which co-elutes with rLHα on Sephadex G-100, a species which elutes with an apparent molecular weight of approximately 21 000 and a large molecular weight form (excluded from Sephadex G-100). Significant quantities of native rLH and rLHα are secreted. The species of large molecular weight binds to Concanavalin A–Sepharose suggesting that it is glycosylated. The 21 000 molecular weight rLH-like molecule has tentatively been identified as an rLH subunit which has not been completely processed.

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