scholarly journals In vitro propagation of the genus Echinacea Moench representatives

2021 ◽  
pp. 49-54
Author(s):  
I. L. Krakhmaleva ◽  
O. I. Molkanova
Keyword(s):  
Root System ◽  
Culture Medium ◽  
Regenerative Capacity ◽  
Morphogenetic Potential ◽  
Effective Development ◽  
Hybrid Cultivar ◽  
Optimal Medium ◽  
Propagation Stage

The regenerative capacity of Echinacea hybrid cultivar Mama Mia and the selected somaclonal variegated variant at the propagation and rooting stages was compared. The influence of the 6-BAP concentration in the MS culture medium on the microrosette formation at the propagation stage was studied. The highest morphogenetic potential of the selected form in comparison with the cultivar Mama Mia was found. It was shown that ½ MS culture medium supplemented with 0.3 mg/L IAA was the optimal medium for effective development of the root system of the genus Echinacea representatives at the rooting stage.

scholarly journals PENGARUH THIDIAZURON DAN HIDROLISAT KASEIN TERHADAP MULTIPLIKASI TUNAS SATOIMO (Colocasia esculenta (L.) Schott var antiquorum ) SECARA IN VITRO

2017 ◽  
Vol 4 (2) ◽  
pp. 17
Author(s):  
. Karyanti ◽  
Minda Kartini
Keyword(s):  
Culture Medium ◽  
Casein Hydrolysate ◽  
Shoot Multiplication ◽  
Colocasia Esculenta ◽  
Optimal Concentration ◽  
Plant Propagation ◽  
In Vitro Multiplication ◽  
Plant Seeds ◽  
Optimal Medium

Effect of Thidiazuron and Casein Hydrolysate on In Vitro Shoot Multiplication of Satoimo (Colocasia esculenta (L.) Schott var antiquorum)ABTRACTSatoimo (Colocasia esculenta (L.) Schott var antiquorum) is an alternative substitute of rice which has a big potential to be developed in Indonesia as an export commodity to Japan. Satoimo production needs to be increased to meet the demands for of the plant seeds. Plant propagation can be done using optimal media to stimulate the formation of shoots through, amongst others, the addition of thidiazuron (TDZ) and casein hydrolysate into the culture medium. This study aimed to determine the optimal concentration of TDZ and casein hydrolysate for in vitro multiplication of satoimo shoots. This research used RAL method with 2 factorials, namely the addition of TDZ at 0; 0.2; 0.6 mg/L concentrations, and of casein hydrolysate at 0; 150; 300; 450 mg/L concentrations. The results showed that the use of 0.6 mg/L TDZ and 150 mg/L casein hydrolysate resulted in the highest number of shoots, with the shoot average number of 6.9 per explant.Keywords: Casein hydrolysate, optimal medium, Satoimo, shoot multiplication, TDZ  ABTRAKSatoimo (Colocasia esculenta (L.) Schott var antiquorum) merupakan salah satu bahan alternatif pengganti beras yang memiliki peluang besar untuk dikembangkan di Indonesia, salah satunya sebagai komoditas ekspor ke negara Jepang. Produksi satoimo perlu ditingkatkan untuk memenuhi kebutuhan bibit tanaman tersebut. Perbanyakan tanaman dapat dilakukan menggunakan media yang optimal untuk merangsang pembentukan tunas, salah satunya dengan penambahan thidiazuron (TDZ) dan hidrolisat kasein pada media tanam. Penelitian ini bertujuan untuk mengetahui konsentrasi TDZ dan hidrolisat kasein yang optimal untuk perbanyakan tunas satoimo secara in vitro. Penelitian ini menggunakan metode RAL dengan 2 faktorial yaitu konsentrasi TDZ yang terdiri dari 0; 0,2; 0,6 mg/L dan konsentrasi hidrolisat kasein yang terdiri dari 0; 150; 300; 450 mg/L. Hasil penelitian menunjukkan bahwa pemberian TDZ 0,6 mg/L dan hidrolisat kasein 150 mg/L menghasilkan jumlah tunas tertinggi, dengan rata-rata tunas yang terbentuk 6,9 per eksplan.Kata kunci: Hidrolisat kasein, multiplikasi tunas, optimasi media, Satoimo, TDZ 

scholarly journals In vitro flowering of Petunia × atkinsiana D. Don

2011 ◽  
Vol 23 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Danuta Kulpa ◽  
Natalia Nowak
Keyword(s):  
Gibberellic Acid ◽  
Root System ◽  
Adventitious Shoots ◽  
In Vitro Flowering ◽  
Ms Medium ◽  
Flowering Initiation ◽  
Number Of Leaves ◽  
Propagation Stage

In vitroflowering ofPetunia × atkinsianaD. DonThe aim of this study was an attempt to determine the influence of PGRs - auxins, cytokinins and gibberellic acid - on the flowering and morphogenesis of ‘Flash Red’Petunia×atkinsianaD. Donin vitro. The best results at the propagation stage were obtained on an MS medium supplemented with 0.5 mg dm-3GA3. The petunia plants propagated under these conditions developed high main shoots and a large number of leaves and adventitious shoots. The plants with the best-developed root system were regenerated on an MS medium supplemented with 0.5 and 1.0 mg dm-3IAA. Flowering initiation should be performed using an MS medium supplemented with 0.5 mg dm-3KIN.

scholarly journals Clonal micropropagation of Thymus vulgaris L.

2020 ◽  
Vol 224 ◽  
pp. 04001
Author(s):  
A Sh Tevfik ◽  
N. A. Yegorova
Keyword(s):  
Plant Material ◽  
Culture Medium ◽  
Culture Media ◽  
Morphometric Parameters ◽  
Thymus Vulgaris ◽  
Ms Medium ◽  
Ex Vitro ◽  
Clonal Micropropagation ◽  
Propagation Stage

Thymus vulgaris L. is one of the widely known spicy aromatic and medicinal plants. Thyme plant material is widely used in medicine, cooking and perfumery. To increase the efficiency of breeding and seed production, it is necessary to develop biotechnological techniques, in particular, clonal micropropagation. The aim of the research is to optimize the composition of culture media for the main stages of propagation in vitro and to select adaptation ex vitro conditions for the development of Thymus vulgaris. clonal micropropagation. The article presents the results of studies of explant morphometric parameters cultivated on 20 variants of culture media at firstsecond stages of micropropagation. It was found that the optimal culture medium at the introduction stage is MS medium with 1.0 mg/l Kin and 1.0 mg/l GA3, on which, on average, 2.2 microshoots per explant with a length of 1.9 cm were obtained. Both high vitrification rate of microshoots and formation of small shoots (0.6-0.9 cm) were observed on media supplemented with BAP or TDZ. The most effective culture medium at the proper propagation stage is MS with 1.0 mg/l Kin, on which 4.6 shoots per explant and the multiplication index 12.8 were obtained. It is advisable to root microshoots at the 3rd stage of micropropagation on MS culture medium supplemented with 1.0 mg/l IBA or 1.0 mg/l IAA. It has been shown that it is possible to obtain high plant survival rate (89.5%) during adaptation ex vitro, using a substrate consisting of peat and perlite (1:1).

scholarly journals A Novel Approach for the Determination of an Optimal Medium for Improving Rapid Micropropagation of Cucurbit Citrullus mucosospermus Fursa

2020 ◽  
pp. 184-192
Author(s):  
Gnamien Yah Gwladys ◽  
Koné Tchoa ◽  
Yao Kouakou Abessika Georges ◽  
Yatty Kouadio Justin ◽  
Zoro Bi Irié Arsène ◽  
...  
Keyword(s):  
Culture Medium ◽  
In Vitro Regeneration ◽  
Mineral Elements ◽  
Shoot Length ◽  
Experimental Planning ◽  
Usual Procedure ◽  
Novel Approach ◽  
Optimal Medium

The search for a suitable medium for In vitro regeneration, whatever the species cultured, is always done by tests including successive steps of removal of components of the culture medium that do not give the expected results. This is the usual procedure. However, the effects of interactions are not taken into account. It is therefore with the aim of taking into account the interactions between the constituents of the culture medium in the formulation of the medium that motivated the present study. Therefore, in order to find the optimal composition with a minimum of experiments or trials, experimental planning was a particularly useful tool. The results of the experiments are analyzed and recommended response values are calculated. A Box-Behnken design wasused by varying three factors: MS mineral elements, sucrose and 6-benzylaminopurine (BAP). Agar concentration has been kept constant. Shoot induction depended on the interaction between levels of sucrose, BAP and MS mineral elements in the medium. The optimum formulation for numerous shoots production is medium containing 1.5 strength of MS mineral elements, 35 g/l sucrose and 1 mg/l BAP solidified with 6 g/l agar. After 3 weeks of culture, 76.7% of the cotyledon explants (proximal parts) regenerated shoots with an average of 12 shoots per explant and a mean shoot length of 17 mm. Compared with the usual procedure, this procedure showed an increment of shoots number per explant and shoot length. The shoots regenerated were directly rooted. Overall, these results make a relevant contribution to the optimization of In vitro propagation of Citrullus mucosospermus Fursa oleaginous type and other hard to propagate related species.

Peculiarities of in vitro propagation of gooseberry varieties

2020 ◽  
Vol 62 ◽  
pp. 105-114
Author(s):  
I. L. Krakhmaleva ◽  
O. I. Molkanova
Keyword(s):  
Culture Medium ◽  
In Vitro Cultivation ◽  
Optimal Size ◽  
High Survival ◽  
Multiplication Rate ◽  
Calcium Hypochlorite ◽  
Optimal Method ◽  
Soil Substrate ◽  
Propagation Stage

The article presents the results of the study on the morphogenesis peculiarities of Ribes uva-crispa L. varieties at all stages of in vitro cultivation and adaptation to ex vitro conditions. The optimal method of sterilization at the initiation stage to in vitro culture was established: consistent application of 2 % «Fundazole» solution with an exposure of 10-15 minutes, 70 % ethanol for 30 seconds and 7 % calcium hypochlorite for 5-7 minutes. The maximum number of viable gooseberry regenerants was obtained in the selection of initial explants during the beginning of active shoots growth (April – early May). The regeneration frequency was 70-80 %, depending on the variety. The optimal size of explants, the mineral and carbohydrate composition of the culture medium, and the cultivation conditions at the propagation stage were determined. The highest multiplication rate was characterized by varieties: Grushenka (6.1-6.7), Beryl (3.1-4.9) and Chernomor (3.5-4.5). It has been established that Quorin and Lepoivre culture medium was the most eff ective medium for the cultivation of the studied gooseberry varieties. As a source of carbohydrates, the use of sucrose at a concentration of 30 g/L was optimal. It was found eff ective to cultivate gooseberries for 20-25 days at a reduced temperature (16-18 °C) at the propagation stage. The efficiency of using 1.0 mg/l IBA at the rooting stage was revealed. The use of sphagnum as part of the soil substrate at the adaptation of gooseberry regenerants contributed to a high survival rate (97-100 %) in all substrate variants. The study of the infl uence of the composition of the soil substrate on the growth dynamics of regenerants showed that the adaptation of gooseberries was eff ectively carried out on a substrate consisting of peat and sphagnum.

Some features of clonal micropropagation of decorative cultivars of Amelanchier Medik.

2020 ◽  
pp. 97-104
Author(s):  
E. N. Raeva-Bogoslovskaya ◽  
O. I. Molkanova
Keyword(s):  
In Vitro Culture ◽  
Culture Medium ◽  
Culture Media ◽  
Culture Conditions ◽  
Clonal Micropropagation ◽  
Morphogenetic Potential

In vitro culture conditions were optimized for representatives of the genus Amelanchier Medik. at the stages of micropropagation and rooting. A significant effect of the mineral and hormonal compositions of culture media on the morphogenetic potential of the cultivars of serviceberry has been established. The use at the stage of micropropagation of the MS culture medium with addition 1,0 mg / L 6-benzylaminopurine (6-BAP) promoted the active microshoot regeneration of the studied genotypes. For the induction of rhizogenesis the type of auxin as IB A at a concentration of 1,0 mg / L was used.

scholarly journals In Vitro Long-Term Expansion and High Osteogenic Potential of Periodontal Ligament Stem Cells: More Than a Mirage

2018 ◽  
Vol 28 (1) ◽  
pp. 129-139 ◽  
Author(s):  
Anna Di Vito ◽  
Amerigo Giudice ◽  
Emanuela Chiarella ◽  
Natalia Malara ◽  
Francesco Bennardo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Periodontal Ligament ◽  
Culture Medium ◽  
Population Doubling ◽  
Osteogenic Potential ◽  
Periodontal Ligament Stem Cells ◽  
Optimal Medium ◽  
Medium Formulation

The periodontal ligament displays a reservoir of mesenchymal stem cells which can account for periodontal regeneration. Despite the numerous studies directed at the definition of optimal culture conditions for long-term expansion of periodontal ligament stem cells (PDLSCs), no consensus has been reached as to what is the ideal protocol. The aim of the present study was to determine the optimal medium formulation for long-term expansion and stemness maintenance of PDLSCs, in order to obtain a sufficient number of cells for therapeutic approaches. For this purpose, the effects of three different culture medium formulations were evaluated on PDLSCs obtained from three periodontal ligament samples of the same patient: minimum essential medium Eagle, alpha modification (α-MEM), Dulbecco’s modified Eagle’s medium (DMEM), both supplemented with 10% fetal bovine serum (FBS), and a new medium formulation, Ham’s F12 medium, supplemented with 10% FBS, heparin 0.5 U/ml, epidermal growth factor (EGF) 50 ng/ml, fibroblast growth factor (FGF) 25 ng/ml, and bovine serum albumin (BSA) 1% (enriched Ham’s F12 medium; EHFM). PDLSCs grown in EHFM displayed a higher PE-CD73 mean fluorescence intensity compared with cells maintained in α-MEM and DMEM, even at later passages. Cells maintained in EHFM displayed an increased population doubling and a reduced population doubling time compared with cells grown in DMEM or α-MEM. α-MEM, DMEM and EHFM with added dexamethasone, 2-phospho-L-ascorbic acid, and β-glycerophosphate were all able to promote alkaline phosphatase activity; however, no calcium deposition was detected in PDLSCs cultured in EHFM-differentiation medium. When EHFM-, α-MEM- and DMEM-expanded PDLSCs were transferred to a commercial culture medium for the osteogenesis, mineralization became much more evident in confluent monolayers of EHFM-expanded PDLSCs compared with DMEM and α-MEM. The results suggest EHFM is the optimal medium formulation for growth and stemness maintenance of primary PDLSCs. Moreover, EHFM confers higher osteogenic potential to PDLSCs compared with cells maintained in the other culture media. Overall, the results of the present work confirmed the advantages of using EHFM for long-term expansion of mesenchymal cells in vitro and the preservation of high osteogenic potential.

scholarly journals Influence of concentration and ratio of macro elements in culture medium on the regeneration of grape meristems in vitro

2020 ◽  
Vol 25 ◽  
pp. 05005
Author(s):  
Anton Rebrov
Keyword(s):  
In Vitro Culture ◽  
Chronic Diseases ◽  
Nutrient Solution ◽  
Culture Medium ◽  
Total Content ◽  
Regenerative Capacity ◽  
Macro Elements ◽  
Grape Varieties

The results of studies on the development of culture medium for initiation grape meristems into in vitro culture in order to increase their regenerative capacity during recovery from chronic diseases are presented. In the improved medium, in comparison with analogs, the total content of macro salts was reduced and their ratio was optimized, taking into account their consumption and the characteristics of their absorption from the nutrient solution by the grape plant. In the course of testing the new culture medium, its effectiveness has been established for grape varieties from various ecological and geographical groups. A higher and more stable percentage of survival and regeneration of extremely small meristems have been established against the background of various modifications of the recipe for the traditionally used culture medium of Murashige and Skoog.

scholarly journals Morphogenesis of Anthers Isolated From Cabbage (Brassica Oleracea L.) In Vitro

2022 ◽  
Author(s):  
Rima Kirakosyan ◽  
Elena Kalashnikova
Keyword(s):  
Culture Medium ◽  
Root Meristem ◽  
Reproductive Organs ◽  
Biologically Active ◽  
Regenerated Plants ◽  
Direct Embryogenesis ◽  
Morphogenetic Potential ◽  
Cytological Method ◽  
Active Substances

This study aimed to optimize the steps of obtaining regenerated cabbage plants by direct embryogenesis from isolated anthers and ovaries. Stepwise pretreatment of inflorescences was usedfor the studied hybrids and inbred lines. First, the inflorescences were placed in water and kept at a temperature of +4-6∘C for one day without the use of biologically active substances. Then the inflorescences were placed in a solution of the drug Dropp (10 mg/l) and cultivated for two days. After that, the anthers and ovaries were isolated from the flower buds and cultured on the MS culture medium at a temperature of + 32∘C for one day. The cultivation of the isolated explants on a nutrient medium (containing 0.01 mg/lof Dropp, 1.0 mg/lof NAA, 500 mg/lof asparagine, 100 mg/l of tyrosine, and 10 g/l of sucrose)led to an increase in their morphogenetic potential in the culture of anthers and ovaries (by 3.42% and 5.54%, respectively).A cytological method was usedto demonstrate the haploid nature of the regenerating plants. The number of chromosomes in the root meristem andleaves, and the chloroplasts in the closing cells of the stomatawere calculated. Keywords: cabbage, culture in vitro, regenerated plants, anthers, ovaries, reproductive organs

Decellularized bone extracellular matrix in skeletal tissue engineering

2020 ◽  
Vol 48 (3) ◽  
pp. 755-764
Author(s):  
Benjamin B. Rothrauff ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Bone Regeneration ◽  
Tissue Regeneration ◽  
Animal Studies ◽  
Tumor Resection ◽  
Regenerative Capacity ◽  
Skeletal Tissue ◽  
Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

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