Isolation of Vibrio Parahaemolyticus from Beef Marketed in Padang, Identification Targeted on ToxR Gene and Amplification of TDH and TRH Genes on Isolates Using Polimerase Chain Reaction

Sebanyak 40 isolat V. parahaemolitycus telah berhasil diisolasi dari sejumlah sampel daging sapi mentah yang dikoleksi di Pasar Raya Padang. Isolasi dilakukan dengan menggunakan media CHROMagarTM Vibrio. Terhadap 40 isolat tersebut dilakukan identifikasi menggunakan metoda Polymerase Chain Reaction (PCR) untuk mengamplifikasi gen toxR. Gen toxR merupakan gen yang sangat spesifik pada spesies V. parahemolyticus. Selanjutnya, dengan metoda yang sama juga dilakukan amplifikasi terhadap gen pengkode produksi toksin hemolisin (tdh dan trh) yang merupakan faktor virulen utama pada bakteri tersebut. Hasil penelitian menunjukkan bahwa seluruh V. parahaemolyticus hasil isolasi memiliki gen tox-R, namun tidak ada satu isolatpun yang memiliki gen pengkode produksi toksin hemolisin baik tdh maupun trh.

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Rapid and sensitive detection of Vibrio parahaemolyticus in sea foods by electrochemiluminescence polymerase chain reaction method

Food Chemistry ◽

10.1016/j.foodchem.2010.05.022 ◽

2010 ◽

Vol 123 (3) ◽

pp. 852-858 ◽

Cited By ~ 13

Author(s):

Jie Wei ◽

Xiaoming Zhou ◽

Da Xing ◽

Baoyan Wu

Keyword(s):

Polymerase Chain Reaction ◽

Vibrio Parahaemolyticus ◽

Polymerase Chain Reaction Method ◽

Sensitive Detection ◽

Chain Reaction ◽

Reaction Method ◽

Polymerase Chain

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Prevalence of Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus, and Salmonella spp. in Seafood Products Using Multiplex Polymerase Chain Reaction

Foodborne Pathogens and Disease ◽

10.1089/fpd.2011.0989 ◽

2012 ◽

Vol 9 (2) ◽

pp. 108-112 ◽

Cited By ~ 37

Author(s):

Mehdi Zarei ◽

Siavash Maktabi ◽

Masoud Ghorbanpour

Keyword(s):

Staphylococcus Aureus ◽

Polymerase Chain Reaction ◽

Listeria Monocytogenes ◽

Vibrio Parahaemolyticus ◽

Multiplex Polymerase Chain Reaction ◽

Chain Reaction ◽

Salmonella Spp ◽

Seafood Products ◽

Polymerase Chain

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Simultaneous multiplex Polymerase Chain Reaction detection of Salmonella spp., Escherichia coli O157, Vibrio parahaemolyticus, Vibrio cholerae, Listeria monocytogenes and Campylobacter spp.

Food Research ◽

10.26656/fr.2017.2(3).002 ◽

2018 ◽

Vol 2 (3) ◽

pp. 240-246

Author(s):

S. Ling ◽

Noramirah R. ◽

Abidatul A.A. ◽

Nurfarhanah N.M.J. ◽

Noor-Azira A.M. ◽

...

Keyword(s):

Escherichia Coli ◽

Polymerase Chain Reaction ◽

Vibrio Parahaemolyticus ◽

Multiplex Polymerase Chain Reaction ◽

Escherichia Coli O157 ◽

Polymerase Chain Reaction Detection ◽

Chain Reaction ◽

Salmonella Spp ◽

Polymerase Chain ◽

Campylobacter Spp

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Detection and Molecular Characterization of Vibrio Parahaemolyticus in Shrimp Samples

The Open Biotechnology Journal ◽

10.2174/1874070701812010046 ◽

2018 ◽

Vol 12 (1) ◽

pp. 46-50 ◽

Cited By ~ 2

Author(s):

Daryoush Asgarpoor ◽

Fakhri Haghi ◽

Habib Zeighami

Keyword(s):

Public Health ◽

Polymerase Chain Reaction ◽

Vibrio Parahaemolyticus ◽

Foodborne Diseases ◽

Chain Reaction ◽

Biochemical Tests ◽

Global Issue ◽

Retail Outlets ◽

Polymerase Chain

Background:Food safety has emerged as an important global issue with international trade and public health implications. Bacterial pathogens asVibrio parahaemolyticusrecognized as an important cause of foodborne diseases related to the consumption of raw, undercooked or mishandled seafood worldwide.Methods:A total of 70 individual wild shrimp samples were collected from shrimp retail outlets in Zanjan, Iran and investigated for the presence of potentially pathogenic strains ofV. parahaemolyticus.The shrimp samples were immediately homogenized and cultured on TCBS agarand subjected to confirmatory biochemical tests. Polymerase Chain Reaction (PCR) was performed for detection of total and pathogenicV. parahaemolyticusby amplification ofvp–toxR,tdhandtrhgenes.Results:The conventional method indicated that 16 (22.8%) of samples were positive forV. parahaemolyticus. However, PCR verified that only 12 (17.1%) shrimp samples were positive forV. parahaemolyticus.Of the 70 shrimp samples in our study, only 2 (2.8%)tdhand 1 (1.4%)trhpositive strains were identified.Conclusion:Detection oftdhand/ ortrhpositiveV. parahaemolyticusin shrimp marketed in Zanjan, Iran shows a probable risk for public health. Therefore, the reliable molecular methods for monitoring of potentially pathogenicV. parahaemolyticusare strongly recommended for the routine seafood examination.

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Application Of The Multiplex Polymerase Chain Reaction (M-PCR) For The Screening Of Vibrio Spp. From Rivers In Kuching, Sarawak

Borneo Journal of Resource Science and Technology ◽

10.33736/bjrst.219.2015 ◽

1970 ◽

Vol 5 (2) ◽

pp. 16-17

Author(s):

Mickey Vincent ◽

Lawrance Tuah ◽

Christy Chan Sien Wei ◽

Lesley Maurice Bilung ◽

Kasing Apun

Keyword(s):

Polymerase Chain Reaction ◽

Vibrio Parahaemolyticus ◽

Multiplex Polymerase Chain Reaction ◽

Vibrio Vulnificus ◽

Simultaneous Detection ◽

Chain Reaction ◽

Single Tube ◽

Predominant Species ◽

Polymerase Chain ◽

Vibrio Spp

The present study was conducted to investigate the occurrence of Vibrio spp. from selected rivers in Kuching,Sarawak (Malaysia) using Multiplex Polymerase Chain Reaction (m-PCR). During the 6-month study period,19 samples were collected monthly from 7 rivers, followed by simultaneous detection of three Vibrio spp.,Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus, in a single tube PCR reaction. Three sets ofprimers targeting the thermolabile (tl), outer membrane protein (ompW) and hemolysin/cytolysin genes(vulCulsl) of V. parahaemolyticus, V. cholerae and V. vulnificus, respectively, were used. The results indicatedthat V. parahaemolyticus was the predominant species, occurring approximately 60.9% throughout thesampling period, followed by V. cholerae (23.1%) and V. vulnificus (16.0%). The months of July andDecember were found to be the months where all three Vibrio spp. were found to be at higher frequencies inthe river samples. Results analyzed also indicated that the rivers with the highest prevalence of the three Vibriospp. were Tambak Sejingkat, followed by Sungai Jernang and Sungai Tabuan. We conclude that m-PCR is apowerful and useful tool for the rapid and simultaneous detection of V. parahaemolyticus, V. cholerae and V.vulnificus from the riverine environments without the need for isolation and culturing. Furthermore, thismethod is highly specific, and could be applied in diagnostic laboratories for larger scale epidemiologicalinvestigations of Vibrio spp.

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