AKTIVITAS ANTIFUNGI DARI EKSTRAK (Mikania micrantha Kunth) TERHADAP Trichophyton mentagrophytes dan Trichophyton rubrum

2021 ◽  
Vol 4 (02) ◽  
pp. 9-19
Author(s):  
Santi Perawati ◽  
Lili Andriani ◽  
Dita Melianti
Keyword(s):  
Trichophyton Rubrum ◽  
Trichophyton Mentagrophytes ◽  
P Value ◽  
Mikania Micrantha

Abstrak Penyakit kulit yang disebabkan oleh jamur masih sering terjadi dibeberapa kalangan masyarakat. Beberapa tumbuhan mempunyai aktivitas sebagai antifungi masih digunakan oleh masyarakat salah satunya sembung rambat (Mikania micrantha Kunth). Penelitian ini bertujuan untuk mengetahui aktivitas antijamur ekstrak Mikania micrantha Kunth terhadap Trichophyton mentagrophytes dan Trichophyton rubrum. Daun Mikania diekstrak  dengan metode maserasi menggunakan pelarut etanol 96% setelah itu ekstrak diidentifikasi kandungan metabolitnya dan diujikan aktivitas antifungi. Pengujian aktivitas antifungi emnggunakan metode difusi cakram dengan konsentrasi ekstrak sebesar 10%, 20% dan 30%. Kontrol positif yang digunakan adalah ketokonazol 15µg/disk dan kontrol negatif berupa DMSO. Analisis data menggunakan SPSS yaitu uji kruskal wallis dan uji Duncan. Hasil penelitian menunjukkan bahwa ekstrak Mikania micrantha  dapat menghambat pertumbuhan   T. mentagrophytes pada konsentrasi 10%, 20%, dan 30% masing-masing sebesar 6,01 mm,6.51 mm dan 7,05 mm. Sementara  pada T.rubrum konsentrasi 10%, 20% dan 30% rata-rata zona hambat masing-masing sebesar 5,68 mm, 6,01 mm, dan 6,51 mm. Hasil uji statistik dengan menggunakan ujikruskal-wallis nilai P-value T.mentagrophytes  sebesar 0.042 dan T.rubrum sebesar 0.087 dan pada uji Duncan hasil yang didapat bahwa tidak ada perbedaan yang signifikasi antara zona hambat dengan konsentrasi ekstrak yang diujikan.   Keywords: Antifungi; Mikania micrantha; Trichophyton mentagrophytes; Trichophyton rubrum.  

scholarly journals Antifungal Agents: Design, Synthesis, Antifungal Activity and Molecular Docking of Phloroglucinol Derivatives

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3116 ◽  
Author(s):  
Xingxing Teng ◽  
Yuanyuan Wang ◽  
Jinhua Gu ◽  
Peiqi Shi ◽  
Zhibin Shen ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Antifungal Activity ◽  
Protein Binding ◽  
Binding Site ◽  
Antifungal Agents ◽  
Trichophyton Rubrum ◽  
Trichophyton Mentagrophytes ◽  
Protein Binding Site ◽  
Antifungal Activities ◽  
Phloroglucinol Derivatives

Pseudoaspidinol is a phloroglucinol derivative with Antifungal activity and is a major active component of Dryopteris fragrans. In our previous work, we studied the total synthesis of pseudoaspidinol belonging to a phloroglucinol derivative and investigated its antifungal activity as well as its intermediates. However, the results showed these compounds have low antifungal activity. In this study, in order to increase antifungal activities of phloroglucinol derivatives, we introduced antifungal pharmacophore allylamine into the methylphloroglucinol. Meanwhile, we remained C1–C4 acyl group in C-6 position of methylphloroglucinol using pseudoaspidinol as the lead compound to obtain novel phloroglucinol derivatives, synthesized 17 compounds, and evaluated antifungal activities on Trichophyton rubrum and Trichophyton mentagrophytes in vitro. Molecular docking verified their ability to combine the protein binding site. The results indicated that most of the compounds had strong antifungal activity, in which compound 17 were found to be the most active on Trichophyton rubrum with Minimum Inhibitory Concentration (MIC) of 3.05 μg/mL and of Trichophyton mentagrophytes with MIC of 5.13 μg/mL. Docking results showed that compounds had a nice combination with the protein binding site. These researches could lay the foundation for developing antifungal agents of clinical value.

scholarly journals Antifungal activity and mechanism of heat-transformed ginsenosides from notoginseng against Epidermophyton floccosum, Trichophyton rubrum, and Trichophyton mentagrophytes

RSC Advances ◽  
2017 ◽  
Vol 7 (18) ◽  
pp. 10939-10946 ◽  
Author(s):  
Peng Xue ◽  
Xiushi Yang ◽  
Xiaoyan Sun ◽  
Guixing Ren
Keyword(s):  
Antifungal Activity ◽  
Trichophyton Rubrum ◽  
Trichophyton Mentagrophytes ◽  
Epidermophyton Floccosum ◽  
Heat Transformation

The improved antifungal activity of notoginseng can be attributed to the formation of less polar ginsenosides by heat transformation.

scholarly journals Frecuencia de dermatomicosis y factores asociados en población vulnerable de la ciudad de Manizales. Colombia. 2011

2016 ◽  
Vol 18 (6) ◽  
pp. 953
Author(s):  
Gloria Ines Estrada Salazar ◽  
José A. Chacón-Cardona
Keyword(s):  
Candida Albicans ◽  
Trichophyton Rubrum ◽  
Culture Media ◽  
Trichophyton Mentagrophytes ◽  
Direct Analysis ◽  
Microsporum Gypseum ◽  
Epidermophyton Floccosum ◽  
Palabras Clave ◽  
Fusarium Sp ◽  
Cutaneous Mycosis

Resumen:Objetivo: Determinar la frecuencia de las dermatomicosis en personas de diferentes instituciones de atención social en la ciudad de Manizales durante el año 2011. Método: Mediante la toma de muestras de los sitios que presentaban algún tipo de lesión sospechosa de ser una micosis cutánea, se hizo un análisis directo con KOH y cultivo en medios de Saboureaud y Mycosel. Se aplicó un instrumento de recolección de información para establecer  factores asociados con la presencia de estos microorganismos.Resultados: Los hongos levaduriformes encontrados con mayor frecuencia fueron: Candida albicans, Trichosporon sp, y los mohos saprofitos Penicillium sp, fusarium sp; seguido de hongos dermatofitos como: Trichophyton mentagrophites, Trichophyton rubrum, Epidermophyton floccosum y Microsporum gypseum. Las lesiones secas y descamativas se encontraron con mayor frecuencia. El compartir baños y vivir en hacinamiento y el uso de elementos comunes fueron los factores asociados más importantes en este estudio.Conclusiones. Las dermatomicosis son frecuentes en poblaciones vulnerables y se asocian a diferentes factores muy similares a los encontrados en otros estudios de igual naturaleza.    Palabras clave: dermatomicosis, onicomicosis, jóvenes, ancianos, factores asociados. Summary:Objective: To determine the frequency of ringworm in people of different social care institutions in the city of Manizales in 2011.Method: Using the sampling sites that had some kind of suspicious lesion from a cutaneous mycosis, direct analysis with KOH and culture media was Saboureaud and Mycosel. An instrument of data collection was used to establish factors associated with the presence of these microorganisms.Results: The yeast found most frequently were: Candida albicans, Trichosporon sp and Penicillium molds saprophytes sp, Fusarium sp, followed by dermatophyte fungi such as Trichophyton mentagrophytes, Trichophyton rubrum, Epidermophyton floccosum and Microsporum gypseum. Dry scaly lesions were found more frequently. The shared bathrooms and living in overcrowded and the use of common elements were the most important in this study associated factors.Conclusions: Dermatomycoses are common in vulnerable populations and are associated with different very similar to those found in other studies of the same nature factors. Keywords: ringworm, onychomycosis, young, old, associated factors.

scholarly journals Dermatofitosis humana en una comunidad con desigualdad social, Ayacucho 2018

2019 ◽  
Vol 27 (1) ◽  
Author(s):  
Serapio Romero Gavilán
Keyword(s):  
Trichophyton Rubrum ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
Microsporum Gypseum

Trabajo de investigación desarrollado con el objetivo de conocer la frecuencia de la dermatofitosis humana en una comunidad con desigualdad social. Hipótesis: la dermatofitosis humana es una afección fúngica muy frecuente en comunidades con desigualdad social. Zona de estudio: comunidad con desigualdad social periurbana de la ciudad de Ayacucho. Tipo de estudio: no experimental. Diseño de estudio: descriptivo-transversal. Muestra: no probabilística, individuos con signos de afecciones compatibles a micosis superficial. Metodología: muestras de escamas de piel, pelos, fragmentos de uñas de pies y manos, escamas de planta, espacios interdigitales y otras partes de cuerpo, fueron tomadas con una hoja de bisturí de filo romo o con el borde de un portaobjetos previa desinfección con alcohol al 70% y colocadas en sobres de papel oscuro etiquetados, en el laboratorio de Epidemiología y Micología de la Universidad Nacional de San Cristóbal de Huamanga, se realizó la observación directa con KOH al 20% y la siembra en placas Petri conteniendo Agar Sabouraud y Agar selectivo para hongos patógenos, después de una incubación a 25ºC por hasta 14 días; las colonias coincidentes con dermatofitos fueron observados al microscopio para identificarlos. Resultados: se ha encontrado que 85/153 (55,5%) presentaron diversas formas de dermatofitosis, no se observó preferencia de la dermatofitosis humana con relación al género (p> 0,05), los factores asociados a la dermatofitosis determinados estadísticamente (p< 0,05) fueron la higiene, el piso de la vivienda y la crianza de animales, se han identificado las especies de Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum canis y Microsporum gypseum.

scholarly journals Anti-TrichophytonActivity of Protocatechuates and Their Synergism with Fluconazole

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Luciana Arantes Soares ◽  
Fernanda Patrícia Gullo ◽  
Janaina de Cássia Orlandi Sardi ◽  
Nayla de Souza Pitangui ◽  
Caroline Barcelos Costa-Orlandi ◽  
...  
Keyword(s):  
Antifungal Agents ◽  
Trichophyton Rubrum ◽  
Biological Activities ◽  
Public Health Problem ◽  
Trichophyton Mentagrophytes ◽  
Fungal Resistance ◽  
Synergistic Activity ◽  
Global Public Health ◽  
Limited Spectrum ◽  
Promising Source

Dermatophytosis and superficial mycosis are a major global public health problem that affects 20–25% of the world’s population. The increase in fungal resistance to the commercially available antifungal agents, in conjunction with the limited spectrum of action of such drugs, emphasises the need to develop new antifungal agents. Natural products are attractive prototypes for antifungal agents due to their broad spectrum of biological activities. This study aimed to verify the antifungal activity of protocatechuic acid, 3,4-diacetoxybenzoic, and fourteen alkyl protocatechuates (3,4-dihydroxybenzoates) againstTrichophyton rubrumandTrichophyton mentagrophytesand to further assess their activities when combined with fluconazole. Susceptibility and synergism assays were conducted as described in M38-A2 (CLSI), with modifications. Three strains ofTrichophyton rubrumand three strains ofTrichophyton mentagrophyteswere used in this work. The pentyl, hexyl, heptyl, octyl, nonyl, and decyl protocatechuates showed great fungicidal effects, with minimum inhibitory concentration (MIC) values ranging from 0.97 to 7.8 mg/L. Heptyl showed a synergistic activity (FIC index= 0.49), reducing the MIC of fluconazole by fourfold. All substances tested were safe, especially the hexyl, heptyl, octyl, and nonyl compounds, all of which showed a high selectivity index, particularly in combination with fluconazole. These ester associations with fluconazole may represent a promising source of prototypes in the search for anti-Trichophytontherapeutic agents.

Sanitization of Contaminated Footwear from Onychomycosis Patients Using Ozone Gas: A Novel Adjunct Therapy for Treating Onychomycosis and Tinea Pedis?

2013 ◽  
Vol 17 (4) ◽  
pp. 243-249 ◽  
Author(s):  
Aditya K. Gupta ◽  
William C. Brintnell
Keyword(s):  
Tinea Pedis ◽  
Trichophyton Rubrum ◽  
Antimicrobial Properties ◽  
Trichophyton Mentagrophytes ◽  
Ozone Exposure ◽  
Adjunct Therapy ◽  
Potential Source

Background: Ozone gas possesses antimicrobial properties against bacteria, viruses, and yeasts. Previously, we demonstrated the efficacy of ozone in killing ATCC strains of the dermatophyte fungi Trichophyton rubrum and Trichophyton mentagrophytes. Objective: To test the efficacy of ozone gas in sanitizing onychomycosis patient footwear contaminated with fungal material as a means of minimizing the risk of reinfection. Methods: Swabs of footwear from onychomycosis patients were cultured prior to and after ozone exposure to test the ability of ozone to sanitize these items. Results: We identified contamination of footwear from most onychomycosis patients, a potential source of reinfection in these individuals. Furthermore, ozone gas was effective in sanitizing contaminated footwear. Conclusion: Ozone gas is effective in sanitizing footwear and represents a novel adjunct therapy to be used in conjunction with antifungal medications and/or devices to better treat onychomycosis and tinea pedis patients in both the short and the long term.

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