scholarly journals Pollen spectrum of the honey of Apis mellifera L. collected in the period of honey production

2019 ◽  
Vol 12 (6) ◽  
pp. 69
Author(s):  
A. M. V. Santos ◽  
A. S. Nascimento ◽  
J. S. Santos ◽  
S. M. P. C. Silva ◽  
C. I. S. Lucas ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Pollen Grains ◽  
Optical Microscope ◽  
Pollen Spectrum ◽  
Honey Production ◽  
Pollen Content ◽  
Pollen Types ◽  
Production Period ◽  
Sample Set

Knowledge of the flora that bees visit is important for characterization of their products and conservation of flora and fauna. Thus, this study identified the flora used by Apis mellifera L. (Hymenoptera: Apidae) during the honey production period through the pollen spectrum. Honey samples were collected at apiaries in the municipality of Cruz das Almas, Bahia, Brazil. International methods for samples preparation were used. ETOH was use for honey dilution and subsequently subjected to acidic treatment by the standard method of acetolysis. The pollen content resulting from the analysis was examined under an optical microscope, with separation and counting of pollen grains. Pollen types were identified by consultation and comparison to reference pollen collections and specialized literature. Twenty-seven pollen types distributed among 19 botanical families were identified in the sample set, and Fabaceae, Rubiaceae, Asteraceae and Sapindaceae were the families with a greater number of pollen types. The most frequent pollen types were Mimosa tenuiflora, Vernonia condensata, Bidens, Hyptis, Amaranthus spinosus, Schefflera morototoni and Serjania pernambucensis, all with relative frequency in samples equal to or greater than 70%. The honey evaluated showed a diverse pollen spectrum, evidencing multifloral characteristic.

scholarly journals Qualitative analysis of Apis mellifera L. honey in an ecotone area in the Bahian semiarid

2021 ◽  
Vol 17 (5) ◽  
Author(s):  
Hélio Souza dos Reis ◽  
Stephanie Oliveira Araújo ◽  
Luciene Cristina Lima e Lima ◽  
Francisco Hilder Magalhães e Silva ◽  
Ivan Jeferson Sampaio Diogo ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Qualitative Analysis ◽  
Pollen Grains ◽  
Frequency Of Occurrence ◽  
Palynological Analysis ◽  
Average Value ◽  
Honey Production ◽  
Standard Procedures ◽  
Pollen Types ◽  
Two Cities

This study aimed to identify the pollen types of 11 honey samples produced in apiaries of two cities in the State of Bahia, Brazil: Antônio Gonçalves (five samples) and Campo Formoso (six samples); to verify richness and diversity from each apiary and to compare the similarity among them. The samples were obtained from beekeepers according to the period of production and availability. The palynological analysis of these samples followed the standard procedures of melissopalynology with the use of acetolysis. The pollen types occurring in the honey samples were identified and at least 1,000 pollen grains per sample were counted to determine the frequency classes and the frequency of occurrence. Overall, 147 pollen types were detected, of which 143 are distributed in 35 botanical families. The most representative families concerning the number of pollen types were: Fabaceae (33), Asteraceae (18), Euphorbiaceae (11), Anacardiaceae and Malvaceae (09 types each), Rubiaceae (08) and Myrtaceae (07). The average value of pollen types per sample was 28.18. The genus that contributed the most was Mimosa L. (Fabaceae) with eight pollen types detected. The wealth of pollen types found revealed important information about the local flora which is explored by Apis mellifera. Therefore, it becomes an important tool to assist in the preservation and propagation of these plants and, consequently, increasing honey production.  

scholarly journals Melliferous flora and pollen characterization of honey samples of Apis mellifera L., 1758 in apiaries in the counties of Ubiratã and Nova Aurora, PR

2013 ◽  
Vol 85 (1) ◽  
pp. 307-326 ◽  
Author(s):  
ELIZABETE S. SEKINE ◽  
VAGNER A.A. TOLEDO ◽  
MARCELO G. CAXAMBU ◽  
SUZANE CHMURA ◽  
ELIZA H. TAKASHIBA ◽  
...  
Keyword(s):  
Glycine Max ◽  
Apis Mellifera ◽  
Native Species ◽  
Ricinus Communis ◽  
Cultivated Plants ◽  
Melia Azedarach ◽  
Native Vegetation ◽  
Pollen Types ◽  
Pollen Analyses

The aim of this study was to carry out a survey of the flora with potential for beekeeping in the counties of Ubiratã and Nova Aurora-PR through the collection of plants and pollen analyses in honey samples collected monthly. 208 species of plants were recorded, distributed in 66 families. The families that showed the major richness of pollen types were: Asteraceae, Myrtaceae and Solanaceae. Approximately 80 pollen types were found in honey samples, most of them were characterized as heterofloral. Cultivated plants, such as Glycine max (soybean) and Eucalyptus spp., were representative in some months of the year. Exotic species, such as Ricinus communis and Melia azedarach, were also frequent. However, over than 50% of the pollen types belong to native species of the region, such as Schinus terebinthifolius, Baccharis spp. Alchornea triplinervia, Parapiptadenia rigida, Hexaclamys edulis, Zanthoxylum sp. and Serjania spp., indicating the importance of the native vegetation for the survival of the colonies.

scholarly journals Pollen spectrum of Finnish honey 

1982 ◽  
Vol 54 (5) ◽  
pp. 403-420
Author(s):  
Anna-Liisa Varis ◽  
Juha Helenius ◽  
Kari Koivulehto
Keyword(s):  
Agricultural Practices ◽  
Pollen Grains ◽  
Weather Conditions ◽  
Pollen Spectrum ◽  
Dominant Type ◽  
Oilseed Crops ◽  
Pollen Types ◽  
Average Proportion ◽  
Total Pollen ◽  
Salix Spp

Pollen spectra were analysed on 160 honey samples representing the whole beekeeping area of Finland in the years 1977 and 1978. A semi-quantitative method of pollen analysis based online counts was developed. It was found efficient for determining the relative numbers of pollen grains in the honey samples. On average, ca. 16 pollen types were identified per sample. The number of honeydew elements was low in all samples. Brassicaceae pollen was the dominant type. The other most frequent and abundant types were pollen grains of Trifolium repens + T. hybridum, Salix spp., "the Rosaceae group” (Prunus, Malus, Sorbus spp.), Apiaceae, and Filipendula ulmaria. These pollen types constituted 90.8 % (1977) and 90.3 % (1978) of all the pollen examined. Between 1960—1963 and 1977—1978 the average proportion of Brassicaceae pollen has risen, while the proportion of T. repens + T. hybridum pollen has decreased, evidently due to the changes in agricultural practices. The seasonal variation in the pollen spectrum was explained by the weather conditions. Regionally characteristic pollen spectra were not found, although Brassicaceae pollen was typical of the honeys of agricultural zones I and II, due to cultivation of Brassica oilseed crops in those regions. The positive correlation of the proportion of Brassicaceae pollen with the total pollen count is discussed in connection with the problem of determining the botanical origin.

scholarly journals Palynological characterization of honey samples from Kwara State, Nigeria

2017 ◽  
Vol 52 (1) ◽  
pp. 7-14
Author(s):  
SD Oyeyemi ◽  
J Kayode
Keyword(s):  
Pollen Grains ◽  
Species Level ◽  
Palynological Analysis ◽  
Genus Level ◽  
Pollen Types ◽  
Family Level

Palynological analysis of honey samples from different localities in Kwara State, Nigeria was carried out to isolate and identified pollen types in the honey samples. Out of forty two pollen types belonging to twenty two botanical families recovered, twenty five were identified to species level, eight to genus level and eight to family level. A total of 849,978 pollen grains were counted with 46,355 in Shao, 101,356 in Ganmo, 22,000 in Idofian, 28,337 in Omupo, 200,090 in Iludun Oro, 298,079 in Ijagbo, 22,100 in Offa I and 131,142 in Afon. The major pollen occurrences in the honey samples include those of Sarcocephaluslatifolius, Parkiabiglobosa, Phyllantusdiscoideus, Tridaxprocubens, Combretaceae/Melastomataceae, Spondiasmombins and Hymenocardiaacida. Other important honey plants identified are Elaeisguineensis, Lanneasp, Parinarisp, Celtissp and Entadaabssynica. All these are characteristic plant taxa of the Forest-Savanna ecotype of the studied area. The presence of relatively high quantity of pollen shows their richness in pollen composition and also a clear evidence that the honey are from botanical sourceand also multifloral.Bangladesh J. Sci. Ind. Res. 52(1), 7-14, 2017

Aeropalinología de la ciudad de Yerba Buena, provincia de Tucumán (Argentina). Aeropalinology of Yerba Buena city, province of Tucumán (Argentina)

2010 ◽  
Vol 35 ◽  
pp. 115-131 ◽  
Author(s):  
María Elena García
Keyword(s):  
Pollen Grains ◽  
Pollen Calendar ◽  
Spore Trap ◽  
Continuous Sampling ◽  
Pollen Types ◽  
Atmospheric Pollen ◽  
Cultivated Species ◽  
Pollen Variation ◽  
The City

Español. Durante los años 1999 - 2002 se llevó a cabo la primera caracterización aerobiológica de la ciudad de Yerba Buena. El muestreo atmosférico se realizó con un captador gravimétrico de tipo Durham. El análisis de las muestras permitió la identificación de 46 tipos polínicos. Los granos de polen más relevantes en el muestreo son de especies cultivadas como Cupressaceae, Morus sp., Broussonetia sp., Fraxinus sp., Liquidambar sp., Salix sp. y Pinus sp. Desde julio a octubre se obtuvo el mayor registro de polen atmosférico con el 70,84 % anual, con una predominancia de granos de polen de especies arbóreas, el 25,05 % de noviembre a abril con un aporte principalmente de granos de polen de especies herbáceas y el 3,57 % de mayo a junio sin contribuciones dominantes de algún tipo polínico. Se elaboró un calendario polínico basado en los 17 tipos de polen de mayor registro atmosférico y se lo comparó con calendarios polínicos obtenidos en ciudades del país. La floración en las Sierra del San Javier en Tucumán, es marcadamente estacional con una acumulación de más de 70% de las especies que florecen en el período primaveral. El muestreo continuado a lo largo de 3 años ha permitido observar que las variaciones polínicas estacionales de la atmósfera de la ciudad de Yerba Buena se hallan en relación con los factores fenológicos y meteorológicos propios de cada año. English.  The first aerobiologic characterization of Yerba Buena city in Tucumán (Argentina) was carried out for the period 1999-2002 using a Durham gravimetric spore trap. The analysis of samples allowed the identification of 46 pollen types. The most outstanding pollen grains in these samples came from cultivated species such as Cupressaceae, Morus sp., Broussonetia sp., Fraxinus sp., Liquidambar sp., Salix sp.and Pinus sp. From July to October the largest atmospheric pollen records were obtained with 70,84% in arboreal species predominantly. From November to April, an important contribution of 25,05% in herbaceous species mainly, and with a 3,57% from May to June without observing predominant contribution of any pollen type. A pollen calendar based on the most frequent 17 pollen types, containing the largest atmospheric records, was elaborated. We compared the pollen calendar obtained with those of other cities in the country. Flowering in the Sierra San Javier at Tucumán is markedly seasonal with an accumulation of over 70% of the species that bloom in the spring period. Continuous sampling during 3 years has revealed that the seasonal pollen variation in the atmosphere of the city of Yerba Buena, is in connection with the phenological and meteorological factors characteristic of every year. 

scholarly journals Pollen analysis of honey and pollen collected by Apis mellifera linnaeus, 1758 (Hymenoptera, Apidae), in a mixed environment of Eucalyptus plantation and native cerrado in Southeastern Brazil

2015 ◽  
Vol 75 (4) ◽  
pp. 821-829 ◽  
Author(s):  
C. M. G. Simeão ◽  
F. A. Silveira ◽  
I. B. M. Sampaio ◽  
E. M. A. F. Bastos
Keyword(s):  
Apis Mellifera ◽  
Pollen Grains ◽  
Southeastern Brazil ◽  
Eucalyptus Plantation ◽  
Cerrado Vegetation ◽  
Pollen Types ◽  
Eucalyptus Plantations ◽  
Northwestern Region ◽  
Copaifera Langsdorffii ◽  
Trema Micrantha

Abstract Eucalyptus plantations are frequently used for the establishment of bee yards. This study was carried on at Fazenda Brejão, northwestern region of the State of Minas Gerais, Brazil. This farm is covered both with native Cerrado vegetation (Brazilian savanna) and eucalyptus plantations. This paper reports on the botanic origin of pollen pellets and honey collected from honeybee (Apis mellifera) hives along a thirteen-month period (January 2004 to January 2005). The most frequent pollen types found in the pollen pellets during the rainy season were Trema micrantha (Ulmaceae), Copaifera langsdorffii (Fabaceae), an unidentified Poaceae, unidentified Asteraceae-2, Cecropia sp. 1 (Cecropiaceae) and Eucalyptus spp. (Myrtaceae); during the dry season the most frequent pollen types were Acosmium dasycarpum (Fabaceae), Cecropia sp. 1 (Cecropiaceae) and Eucalyptus spp. (Myrtaceae). Pollen grains of Baccharis sp. (Asteraceae), Cecropia sp. 1 (Cecropiaceae), Copaifera langsdorffii (Fabaceae), Mimosa nuda (Fabaceae), Eucalyptus spp. (Myrtaceae) and Trema micrantha (Ulmaceae) were present in the honey samples throughout the study period.

scholarly journals Purification of Hollow Sporopollenin Microcapsules from Sunflower and Chamomile Pollen Grains

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2094
Author(s):  
Jose Manuel Ageitos ◽  
Sandra Robla ◽  
Lorena Valverde-Fraga ◽  
Marcos Garcia-Fuentes ◽  
Noemi Csaba
Keyword(s):  
Drug Delivery ◽  
Pollen Grains ◽  
Morphological Characterization ◽  
Product Density ◽  
Low Protein ◽  
Pollen Types ◽  
Delivery Platform ◽  
Nanoporous Surface ◽  
First Time

Pollen grains are natural microcapsules comprised of the biopolymer sporopollenin. The uniformity and special tridimensional architecture of these sporopollenin structures confer them attractive properties such as high resistance and improved bioadhesion. However, natural pollen can be a source of allergens, hindering its biomedical applicability. Several methods have been developed to remove internal components and allergenic compounds, usually involving long and laborious processes, which often cannot be extended to other pollen types. In this work, we propose an abridged protocol to produce stable and pristine hollow pollen microcapsules, together with a complete physicochemical and morphological characterization of the intermediate and final products. The optimized procedure has been validated for different pollen samples, also producing sporopollenin microcapsules from Matricaria species for the first time. Pollen microcapsules obtained through this protocol presented low protein content (4.4%), preserved ornamented morphology with a nanoporous surface, and low product density (0.14 g/cm3). These features make them interesting candidates from a pharmaceutical perspective due to the versatility of this biomaterial as a drug delivery platform.

scholarly journals Influence of changes in crop cultivation areas on pollen contents of honey (Research Note)

2000 ◽  
Vol 9 (3) ◽  
pp. 253-258 ◽  
Author(s):  
A.-L. VARIS
Keyword(s):  
Pollen Grains ◽  
Late Summer ◽  
Rubus Idaeus ◽  
Meadow Fescue ◽  
Pollen Counts ◽  
Oilseed Crops ◽  
Pollen Content ◽  
Pollen Types ◽  
The 1960S ◽  
Salix Spp

Pollen counts were done on honey collected by a Finnish honey corporation in late summer 1997 from the entire beekeeping area of Finland. The most common pollen type was Brassicaceae pollen, which was represented by 60% of the grains counted. It was followed by Salix spp. (10%), Trifolium repens + T. hybridum (10%) and T. pratense + T. medium (6.5%) species. Pollen grains of Phacelia spp, Filipendula ulmaria, Apiaceae, Sorbus aucuparia, Malus domestica, and Rubus idaeus were also numerous. These pollen types constituted 96% of all the pollen examined. These results and those of the earlier pollen counts in Finland were compared with the cultivation areas of the most important nectariferous crops. In the 1930s white clover was the most important honey source in Finland and its pollen was very dominant in honey. Since the 1950s oilseed crops have been grown in increasing rates and pure timothy-meadow fescue pastures and hay stands with heavy N applications have decreased the share of Trifolium species. The proportion of Brassicaceae pollen has continuously increased with the increase of the growing area of turnip rape Brassica rapa ssp. oleifera and rape, B. napus ssp. oleifera. At the same time the proportion of T. repens + T. hybridum pollen has decreased so that their mutual relationships are now reversed compared to the beginning of the 1960s. Changes in land use were thus very clearly to be seen in the pollen content of honey.;

scholarly journals Geographical origin of white honey produced by stingless bees in the Araucaria Forest in Southern Brazil

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sidia Witter ◽  
Letícia Azambuja Lopes ◽  
Claudia Inês Silva ◽  
Bruno Brito Lisboa ◽  
Vera Lucia Imperatriz-Fonseca ◽  
...  
Keyword(s):  
Stingless Bees ◽  
Geographical Origin ◽  
Pollen Grains ◽  
Southern Brazil ◽  
Araucaria Forest ◽  
Forest Region ◽  
Pollen Types ◽  
Floral Odor ◽  
Melipona Species ◽  
Production Period

Abstract: Honey produced by native stingless bees in the Araucaria Forest region of Rio Grande do Sul, southern Brazil, specifically the municipality of Cambará do Sul, is highly valued for its characteristic white color, floral odor and taste. In this study, we investigated the botanical origin of white honey stored in colonies of five Meliponini species of the genera Melipona (n = 3) and Plebeia (n = 2). During the production period of white honey, from January to March, flowers were sampled fortnightly along pre-established trails to identify plants used by bees. For all sampled plant species, exsiccates and pollen reference slides were prepared. Honey samples from stingless bees were processed for extraction and preparation of pollen grains for identification of pollen types. In all analyzed honey samples, pollen grains of Clethra scabra (Clethraceae) were predominant (between 46-94%). Pollen grains from other botanical families, including Myrtaceae, Fabaceae and Melastomataceae were frequently identified in honey samples of the Melipona species, while Cunoniaceae was also found in samples of Plebeia species. In this study, we concluded that Clethra scabra is predominantly used by Meliponini bees in the production of white honey in the municipality of Cambará do Sul.

scholarly journals Melissopalynology Reveals the Foraging Preferences of the Stingless Bee Melipona seminigra pernigra Moure & Kerr 1950 (Apidae: Meliponini) in Cangas of Serra dos Carajás, Southeastern Amazonia

2019 ◽  
Author(s):  
José Tasso Felix Guimarães ◽  
Luciano Costa ◽  
Daniela Cristina Zappi ◽  
Wilson Filgueira Batista Junior ◽  
Karen da Silva Lopes ◽  
...  
Keyword(s):  
Pollen Grains ◽  
Floral Resources ◽  
Campo Rupestre ◽  
Foraging Preferences ◽  
Disturbed Areas ◽  
Pollen Content ◽  
Degraded Area ◽  
Significant Difference ◽  
Pollen Types ◽  
Local Variability

The pollen content of honey samples collected in the years 2017 and 2019 from experimental apiaries of Melipona seminigra pernigra Moure & Kerr 1950 installed in campo rupestre on canga (CRC) vegetation of the Serra dos Carajás, southeastern Amazonia, was analyzed to understand the local variability of floral resources occurring on natural and disturbed areas. Around one hundred pollen types were identified mainly belonging to Fabaceae, Myrtaceae and Euphorbiaceae (31, 6 and 5 types, respectively). The N5 mine presented the highest pollen richness with 95 pollen types identified, almost twice of those identified in the other areas, including the better preserved ones. Eighty percent of the pollen types are rare with concentrations ≤ 2,000 pollen grains/10 g; the remaining types are the most abundant and frequent, and are considered the primary bee sources (PBS). PBS correspond mostly to native plants such as Tapirira guianensis Aubl., Protium spp., Aparisthmium cordatum (A.Juss.) Baill., Mimosa acutistipula var. ferrea Barneby, Periandra mediterrânea (Vell.) Taub., Miconia spp., Pleroma carajasense K.Rocha, Myrcia splendens (Sw.) DC., Serjania spp. and Solanum crinitum Lam. All pollen types were identified during both seasons, but higher pollen concentration are related to the dry period (June-September). The statistical analysis indicated that there was no significant difference in honey pollen data between the natural and disturbed areas since the plant species considered as PBS in this work are intensively used in revegetation of degraded area (RDA) processes by mining activities.

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