scholarly journals Traditional ethnoveterinary knowledge of indigestion or diarrhoea treatments in cattle on the Bilogora hills in Croatia

2021 ◽  
pp. 25-34
Author(s):  
Drazen Duricic ◽  
Marko Samardzija

Traditional knowledge of plants and their preparations used for the treatment of animal diseases was passed down orally from generation to generation, so there are no written records or they are very rare. This study is based on the first documentation of ethnoveterinary knowledge for indigestion or diarrhoea treatment in cattle on the Bilogora hills in northwestern Croatia. Data collection was conducted from 2008 until 2018, in eighteen villages of four municipalities in the Koprivni?ko-križeva?ka county, Croatia. Plant specimens were well known, in addition they were confirmed and identified by the skilled botanist. Nine plant species: flax (Linum usitatissimum L.), chamomile (Matricaria recutita L.), hazelwort (Asarum europaeum L.), broad-leaved dock (Rumex obtusifolius L.), sweet chestnut (Castanea sativa Mill.), common oak (Quercus robur L.), white willow (Salix alba L.), common mallow (Malva sylvestris L.), yarrow (Achillea millefolium L.) from 7 botanical families were documented. Decoction and herbal tea were the most common preparation methods. The most often used plants to treat mild diarrhea in cattle were chamomile and broad-leaved dock, and for hard, watery diarrhoea bark of sweet chestnut and sessile oak. The most often used plants to treat indigestion in cattle were hazelwort, chamomille, and flaxseed. Farmers used mostly leaves (about 57%), flowers and stems (more than 25%), bark (about 13%), branches, and seeds (5%) for herbal preparations. Thus the aim of the present study is to document that ethnoveterinary tradition for the next generations. Keywords: Cattle; Diarrhoea; Ethnoveterinary; Indigestion

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1538
Author(s):  
Ana Teresa Alhinho ◽  
Miguel Jesus Nunes Ramos ◽  
Sofia Alves ◽  
Margarida Rocheta ◽  
Leonor Morais-Cecílio ◽  
...  

The sweet chestnut tree (Castanea sativa Mill.) is one of the most significant Mediterranean tree species, being an important natural resource for the wood and fruit industries. It is a monoecious species, presenting unisexual male catkins and bisexual catkins, with the latter having distinct male and female flowers. Despite the importance of the sweet chestnut tree, little is known regarding the molecular mechanisms involved in the determination of sexual organ identity. Thus, the study of how the different flowers of C. sativa develop is fundamental to understand the reproductive success of this species and the impact of flower phenology on its productivity. In this study, a C. sativa de novo transcriptome was assembled and the homologous genes to those of the ABCDE model for floral organ identity were identified. Expression analysis showed that the C. sativa B- and C-class genes are differentially expressed in the male flowers and female flowers. Yeast two-hybrid analysis also suggested that changes in the canonical ABCDE protein–protein interactions may underlie the mechanisms necessary to the development of separate male and female flowers, as reported for the monoecious Fagaceae Quercus suber. The results here depicted constitute a step towards the understanding of the molecular mechanisms involved in unisexual flower development in C. sativa, also suggesting that the ABCDE model for flower organ identity may be molecularly conserved in the predominantly monoecious Fagaceae family.


2020 ◽  
Vol 10 (20) ◽  
pp. 11192-11216
Author(s):  
Paola Pollegioni ◽  
Stefano Del Lungo ◽  
Ruth Müller ◽  
Keith E. Woeste ◽  
Francesca Chiocchini ◽  
...  

2016 ◽  
Vol 11 (3) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Margherita Campo ◽  
Patrizia Pinelli ◽  
Annalisa Romani

Sweet Chestnut ( Castanea sativa Mill.) wood extracts, rich in Hydrolyzable Tannins (HTs), are traditionally used in the tanning and textile industries, but recent studies suggest additional uses. The aim of this work is the HPLC-DAD-ESI-MS characterization of Sweet Chestnut aqueous extracts and fractions obtained through a membrane separation technology system without using other solvents, and the evaluation of their antioxidant and antiradical activities. Total tannins range between 2.7 and 138.4 mM; gallic acid ranges between 6% and 100%; castalagin and vescalagin range between 0% and 40%. Gallic Acid Equivalents, measured with the Folin-Ciocalteu test, range between 0.067 and 56.99g/100g extract weight; ORAC test results for the marketed fractions are 450.4 and 3050 μmol/g Trolox Equivalents/extract weight. EC50 values, measured with the DPPH test, range between 0.444 and 2.399 μM. These results suggest a new ecofriendly and economically sustainable method for obtaining chestnut fractions with differentiated, stable and reproducible chemical compositions. Such fractions can be marketed for innovative uses in several sectors.


2019 ◽  
Vol 143 (9-10) ◽  
pp. 469-477
Author(s):  
Damird Drvodelić ◽  
Igor Poljak ◽  
Marilena Idžojtić ◽  
Ivana Zegnal ◽  
Katarina Tumpa ◽  
...  

U radu su prikazani rezultati ispitivanja laboratorijske klijavosti i morfoloških značajki klijanaca europskoga pitomoga kestena (Castanea sativa Mill.). Uzorci za istraživanja sakupljeni su u submediteranskom području Hrvatske, a radni uzorak za ispitivanje klijavosti iznosio je 8×25 sjemenki. Sjeme je do ispitivanja klijavosti čuvano tri mjeseca u hladnjaku na temperaturi od 3 °C. Postotak laboratorijske klijavosti utvrđen je prema postotku pravilnih klijanaca koji su normalno proklijali nakon 27 dana ispitivanja. Ispitivanje je provedeno u laboratoriju prema uvjetima koje propisuje ISTA (International Seed Testing Association). Izrađen je katalog sa slikama i opisom nepravilnih klijanaca. Na osnovi definiranih kodova nepravilnih klijanaca moguće je preventivno djelovati kako bi se reducirale nepoželjne jedinke. Rezultati ovog istraživanja od velike su koristi za razumijevanje složenoga procesa klijanja sjemena, kako u laboratoriju, tako i šumskim rasadnicima.


2021 ◽  
pp. 2427-2433
Author(s):  
Tipwadee Prapaiwong ◽  
Wuttikorn Srakaew ◽  
Chalong Wachirapakorn ◽  
Chaiwat Jarassaeng

Background and Aim: Hydrolyzable tannins are an important group of secondary plant metabolites, which are known for antimicrobial activity. This study aimed to assess the efficiency with which a hydrolyzable tannin extract from sweet chestnut wood (Castanea sativa Mill.) could inhibit mastitis-causing bacteria in vitro. Materials and Methods: The negative control used was sterile water, and the positive controls were penicillin and gentamicin. The treatments included five concentrations of hydrolyzable tannins (63, 190, 313, 630, and 940 mg/mL). In cows with subclinical mastitis, the bacteria causing the disease were isolated and identified. Then, the antibacterial activity of the hydrolyzable tannin extract was assessed by the disk diffusion method, by determining the minimum inhibitory concentration (MIC) and by determining the minimum bactericidal concentration (MBC). Results: Penicillin inhibited (p<0.01) the growth of Staphylococcus aureus, Streptococcus uberis, and Pseudomonas aeruginosa but could not inhibit (p>0.05) the growth of Streptococcus agalactiae, Escherichia coli, and Klebsiella pneumoniae. However, gentamicin and hydrolyzable tannins could inhibit (p<0.01) all isolated bacteria. Increasing the concentration of hydrolyzable tannin extract resulted in a quadratic increase in the inhibition zone diameter of S. aureus and S. agalactiae and a linear increase in the inhibition zone diameter of E. coli, K. pneumoniae, and P. aeruginosa. In addition, 630 and 940 mg/mL of hydrolyzable tannin extract showed the highest antibacterial activity against S. agalactiae and E. coli (p<0.01), while 940 mg/mL concentration had the highest antibacterial activity against K. pneumoniae (p<0.01). The MIC and MBC of the extract were 27.3-190 mg/mL and 58.8-235 mg/mL, respectively, with the MBC: MIC ratio being 2:1. Conclusion: The antimicrobial activity of the hydrolyzable tannin extract against subclinical mastitis bacteria was comparable to the antibiotics (positive controls) at concentrations over 630 mg/mL. Although these in vitro findings are promising, further research is needed to determine whether hydrolyzable tannins could be used to control or prevent subclinical mastitis in dairy cows.


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