Physicochemical Characteristics of Coconut Water from “in vitro Culture” (Cocos nucifera L.) According to Fruit Maturation Stage and Storage Period

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%) dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan

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Antibacterial efficacy of tender coconut water (Cocos nucifera L) on Streptococcus mutans: An in-vitro study

Journal of International Society of Preventive and Community Dentistry ◽

10.4103/jispcd.jispcd_275_16 ◽

2017 ◽

Vol 7 (2) ◽

pp. 130 ◽

Cited By ~ 3

Author(s):

JN Rukmini ◽

Sunkari Manasa ◽

Chenna Rohini ◽

LavanyaPutchla Sireesha ◽

Sachan Ritu ◽

...

Keyword(s):

Streptococcus Mutans ◽

Cocos Nucifera ◽

In Vitro Study ◽

Vitro Study ◽

Coconut Water ◽

Antibacterial Efficacy ◽

Tender Coconut Water ◽

Cocos Nucifera L ◽

Tender Coconut

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IMPACT OF VARIOUS 2, 4-D CONCENTRATIONS AND DIFFERENT VITAMIN MIXTURES ON in vitro CULTURE OF COCONUT (Cocos nucifera L.) BY UTILIZING SEEDLINGS SHOOT TIP

Acta Scientiarum Polonorum Hortorum Cultus ◽

10.24326/asphc.2020.2.13 ◽

2020 ◽

Vol 19 (2) ◽

pp. 135-145

Author(s):

Sherif Fathy ElGioushy ◽

Liu Rui ◽

Haikuo Fan

Keyword(s):

In Vitro Culture ◽

Cocos Nucifera ◽

Shoot Tips ◽

Shoot Tip ◽

Survival Percentage ◽

High Concentrations ◽

Cocos Nucifera L ◽

The Impact ◽

Vitamin Mixture

In this experiment, seedlings as sources for shoot tips was assembled from the coconut research institute (CRI) of Chinese Academy of tropical agricultural sciences germplasm, Wenchang, Hainan, China. The shoot tips were cultured on Y3 medium to study the impact of ten concentrations of 2, 4-D and three vitamin mixtures on survival, death and development % on in vitro culture establishment stage of coconut (Cocos nucifera L.). The obtained results revealed that all 2,4-D concentrations 4,7, 75, 100, 120 and 140 mg/L + any vitamin mixture did not give any development and high concentrations of 2, 4-D increased the death %. In any case, it can be concluded that the rate of differences in survival, death and development percentages exhibited by 2, 4-D concentrations was more pronounced than the analogous ones resulted by vitamin mixtures. At any rate, utilizing of (2, 4-D at concentration 25 mg/L+ vitamin mixture 1 or 2) exhibited the greatest values of survival percentage and reduced death percentage. Just as, increased the percentages of development in in vitro culture establishment stage of coconut (Cocos nucifera L.) through seedlings shoot tip.

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Antimicrobial Properties of Cocos nucifera: A Review

CORD ◽

10.37833/cord.v31i1.62 ◽

2015 ◽

Vol 31 (1) ◽

pp. 6

Author(s):

Lalitha Ramaswamy

Keyword(s):

Cocos Nucifera ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Oral Bacteria ◽

Coconut Water ◽

Annual Data ◽

Alcoholic Extract ◽

Medicinal Plant Extracts ◽

Cocos Nucifera L

Akinyele, T.A., 2011. Assessment of the antibacterial properties of n-Hexane extract of Cocos Nucifera and its interactions with some Conventional antibiotics. Masters Dissertation. University of Fort Hare, Alice. Alan´ıs, A.D., Calzada, F, Cervantes J.A., Torres, J., and Ceballos, G.M. 2005. Antibacterial properties of some plants used in Mexican traditional medicine for the treatment of gastrointestinal disorders, Journal of Ethnopharmacology. 100, 153–157. Alviano, W.S., Alviao, D.S., Diniz, C.G., Antoniolli, A.R., Alviano, C.S., Frias, L.M. 2008. In vitro antioxidant potential of medicinal plant extracts and their activities against oral bacteria based on Brazilian folk medicine. Arch Oral Biol. 53:545-552. Arora, R, Chawla, R, Marwah, R, Arora, P, Sharma R.K., Kaushik, V, Goel, R, Kaur, A, Silambarasan, M, Tripathi, R.P., and Bhardwaj, J.R. 2011. Corporation. Evidence-Based Complementary and Alternative Medicine. Hindawi Publishing. Bakhru, H.K. 2000. Foods That Heal. Orient Paper Backs, New Delhi. Batovska, I.D., Todorova, I.T., Tsvetkova I.V., and Najdenski H.M. 2009. Antibacterial Study of the Medium Chain Fatty Acids and Their 1- Monoglycerides: Individual Effects and Synergistic Relationships. Polish Journal of Microbiology. 58: 43- 47. Bolling, B.W., McKay, D.L., Blumberg, J.B. 2010. The phytochemical composition and antioxidant actions of tree nuts. Asia Pac J Clin Nutr; 19: 117-23. Conrado S. Dayrit. 2000. Read at the XXXVII Cocotech Meeting, Chennai, India Deb Mandal M, Mandal. S 2011. Coconut (Cocos nucifera L.: Arecaceae): In health promotion and disease Prevention. Asian Pacific Journal of Tropical Medicine, 241-247. Dyana, J.P., and Kanchana, G. 2012. Preliminary Phytochemical Screening of Cocos Nucifera L. Flowers, International Journal of Current Pharmaceutical Research, Vol 4, Issue 3. Effiong, G.S., Ebong, P.E., Eyong, E.U., Uwah, A.J., and Ekong, U.E. 2010. Amelioration of Chloramphenicol Induced Toxicity in Rats by Coconut Water, Journal of Applied Sciences Research, 6(4): 331-335. Esquenazi D, Wigg M.D, Miranda M.M.F.S., Rodrigues H.M., Tostes J.B.F., Rozental S, da Silva A.J.R. and Alviano, C.S 2002. Antimicrobial and antiviral activities of polyphenolics fromCocos nucifera Linn. (Palmae) husk fiber extract. Research in Microbiology 153: 647–652. Food and Agricultural Organization of the United Nations, Economic and Social Department. Statistics division (September 2, 2010). FAOSTAT- Production- Crops [Selected annual data]. Retrieved April 14, 2011 from the FAOSTAT database. Ifesan, B.O.T., Fashakin, J.F., Ebosele, F, and Oyerinde, A.S. 2013. Antioxidant and Antimicrobial Properties of Selected Plant Leaves, European Journal of Medicinal Plants 3(3): 465-473. Krishnamoorthy, M. and Arjun, P. 2012. Probiotic and antimicrobial activity of bacteria from fermented toddy of Cocos nucifera, J. Acad. Indus. Res. Vol. 1(3). Mandal S.M., Dey, S, Mandal, M, Sarkar, S, Maria-Neto, S. and Franco, O.L. 2009. Identification and structural insights of three novel antimicrobial peptides isolated from green coconut water. Peptides. 30. 633-637. Mariselvam, R, Ranjitsingh, A.J.A., Nandhini, U.R.A. and Kalirajan, K. 2013. Antihelmintic and antibacterial activity of Cocos nucifera tree inflorances crude extract. IJSID, 3 (2), 311-316. Mehlhorn H, Al-Quraishy S, Al-Rasheid KAS, Jatzlau A, and Abdel-Ghaffar F. Addition of a combination of onion (Allium cepa) and coconut (Cocos nucifera) to food of sheep stops Gastrointestinal Helminthic infections. Parasitol Res (2011) 108:1041–1046. Mendonça-Filho R.R, Rodrigues I.A, Alviano D.S, Santos A.L.S, Soares R.M.A, Alviano C.S, Lopes A H.C.S., Rosa M.S. 2004. Leishmanicidal activity of polyphenolic-rich extract from husk fiber of Cocos nucifera Linn. (Palmae) Research in Microbiology 155: 136–143. Mukherjee PK, Kumar SN and Heinrich M (2008). Plant Made Pharmaceuticals (PMPs)- Development of Natural Health Products from Bio-Diversity. Indian J. Pharm Educ. Res 42(2), 113-121. Nakatsuji, T, Kao M.C., Fang, J.Y., Zouboulis, C.C., Zhang, L, Gallo R.L. and Huang C.M. 2009. Antimicrobial Property of Lauric Acid against Propionibacterium acnes: Its Therapeutic Potential for Inflammatory Acne Vulgaris, Journal of Investigative Dermatology, Volume 129. NMCE. Report on Copra. National Multi-Commodity Exchange of India Limited; 2007, 1-14. O’Neil, C.E., Keast, D.R., Nicklas, T.A. and Fulgoni V.L. 2012. Out of-hand nut consumption is associated with improve nutrient intake and health risk markers in US children and adults: National Health and Nutrition Examination Survey 1999-2004. Nutr Res 32: 185–194. Ogbolu D.O., Oni AA, Daini OA, and A.P. Oloko 2007. In Vitro Antimicrobial Properties of Coconut Oil on Candida Species in Ibadan, Nigeria, J Med Food 10 (2), 384–387. Pushpan R, Kumari H, Nishteswar K and N Vishwanathan. 2013. Preliminary Phytochemcial Screening of Narikelapushpa (Flower of Cocos nucifera L.) Global Journal of Traditional Medicinal Systems, 2(2): 1-3. Singla R.K., Jaiswal N, Bhat V and Hitesh Jagani 2011. Antioxidant & Antimicrobial Activities of Cocos Nucifera Linn. (Arecaceae) Endocarp Extracts Indo Global Journal of Pharmaceutical Sciences; 1(4): 354-361. Taheri J.B., Espineli F.W., Lu H, Asayesh M, Bakshi M, Nakhostin M.R 2010. Antimicrobial effect of coconut flour on oral microflora: An in vitro study. Res J Biol Scs. 5(6): 456-459. Thaweboon S, Nakaparksin J, Thaweboon B.2011. Effect of Oil-Pulling on Oral Microorganisms in Biofilm Models, Asia Journal of Public Health, Vol. 2 No. 2. Venkataraman S, Ramanujam T.R, Venkatasubbu V.S. 1980. Antifungal activity of the alcoholic extract of coconut shell—Cocos nucifera Linn. J. Ethnopharmacol. 2: 291–293. Verma V, Bhardwaj A, Rathi S. and Raja R.B 2012. A Potential Antimicrobial Agent from Cocos nucifera mesocarp extract; Development of a New Generation Antibiotic. ISCA Journal of Biological Sciences. Vol. 1(2), 48-54.

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Pengaruh Air Kelapa (Cocos nucifera L) dengan Medium VW terhadap Pertumbuhan Protocorm Anggrek secara in vitro

Jurnal SAINS ALAMI (Known Nature) ◽

10.33474/j.sa.v3i1.7208 ◽

2020 ◽

Vol 3 (1) ◽

Author(s):

Edi Santoso ◽

Tintrim Rahayu ◽

Ari Hayati

Keyword(s):

Water Treatment ◽

Cocos Nucifera ◽

Coconut Water ◽

Network Culture ◽

Randomized Design ◽

Completely Randomized Design ◽

Commercial Value ◽

Cocos Nucifera L

Phalaenopsis sp is one of the types of orchids native to Indonesia with high commercial value, but these orchids are quite difficult to cultivate because the seeds are microscopic and do not have endosperm that need to be cultivated in vitro. In vitro orchid cultivation requires a suitable supporting medium. This study aims to determine the effect of coconut water (Cocos nucifera L) using VW medium on the growth of Phalaenopsis sp protocorm orchid in vitro. Research has been carried out at the DD Orchid Nursery Network Culture Laboratory Dadaprejo Junrejo, Batu, East Java, starting from November to January 2020. This study uses a Completely Randomized Design (CRD) to compare different concentrations of coconut water (Cocos nucifera L) as ie, 0%, 15%, 30%, and 60% treatment. Each concentration was repeated 5 times and each repetition consisted of 5 bottles of Phalaenopsis sp. Protocorm and cultured for 4 weeks. The results showed that the highest number of protochromes and shoots were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average number of 64 protocorms and 14 shoots. Keywords: Coconut water (Cocos nucifera L), Phalaenopsis sp., In vitro, growthABSTRAKPhalaenopsis sp merupakan salah satu jenis anggrek asli Indonesia dengan nilai komersial yang tinggi, tetapi anggrek ini cukup sulit dibudidayakan karena bijinya bersifat mikroskopis dan tidak memiliki endosperm sehingga perlu dibudidayakan secara in vitro. Budidaya anggrek secara in vitro memerlukan medium pendukung yang sesuai. Penelitian ini bertujuan untuk mengetahui pengaruh air kelapa (Cocos nucifera L) menggunakan medium VW terhadap pertumbuhan protocorm anggrek Phalaenopsis sp secara in vitro. Telah dilakukan penelitian di Laboratorium Kultur Jaringan DD Orchid Nursery Dadaprejo Junrejo Kabupaten Batu Jawa Timur, dimulai dari bulan November sampai dengan bulan Januari 2020. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) untuk membandingkan beberapa konsentrasi air kelapa (Cocos nucifera L) yang berbeda sebagai perlakuan yaitu, 0 %, 15%, 30%, dan 60 %. Masing-masing konsentrasi dilakukan 5 kali pengulangan dan setiap pengulangan terdiri dari 5 botol protocorm Phalaenopsis sp dan dikultur selama 4 minggu. Hasil penelitian menunjukkan bahwa jumlah protokrom dan tunas terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%) dengan rata-rata jumlah 64 protocorm dan 14 tunas.Kata kunci : Air kelapa (Cocos nucifera L), Phalaenopsis sp., in vitro, pertumbuhan.

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