A comparison of genetic diversity between wild and cultured populations of the Chinese giant salamander,Andrias davidianus, based on microsa-tellite analyses

2008 ◽  
Vol 16 (6) ◽  
pp. 533
Author(s):  
Meng Yan ◽  
Yang Yanqing ◽  
Zhang Yan ◽  
Xiao Hanbing
Keyword(s):  
Genetic Diversity ◽  
Andrias Davidianus ◽  
Chinese Giant Salamander

scholarly journals Characterization of Single Nucleotide Polymorphism Marker in the Chinese Giant Salamander Andrias davidianus

2021 ◽  
Author(s):  
Quanhe Wang ◽  
Lei Cheng ◽  
Haifeng Tian ◽  
Xiaoli Dong ◽  
Hanbing Xiao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Nutritional Value ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Andrias Davidianus ◽  
Allele Number ◽  
Expected Heterozygosity ◽  
Hardy Weinberg Equilibrium ◽  
Chinese Giant Salamander

Background: Chinese giant salamander Andrias davidianus is called “baby fish” in China. Due to its palatability and nutritional value, it was widely cultured in China. The current study was to evaluate the genetic diversity of the farmed A. davidianus. Methods: 30 salamanders were collected from farm and DNA was extracted. Thirty contigs potentially containing SNPs were selected from the previously developed RAD-seq library. The primer was designed and PCR was performed, than the products was sequenced from different direction. At last the sequences were analyzed using the vector NTI 10.Result: 5824bp high-quality sequences embodied in 16 contigs were sequenced and eighty SNP loci were identified which each SNP locus exhibited bi-alleles. The effective allele number from 1.03 to 2.00 and the minor allele frequency was 0.017 to 0.500. The expected heterozygosity was from 0.0333 to 0.5091 and the observed heterozygosity was 0.0333 to 1.0000. Sixty-six of the 80 loci significantly deviated from the Hardy-Weinberg equilibrium (P less than 0.05). The results provide useful genomic resources to evaluate the genetic diversity of A. davidianus for conservation and aquaculture.

Genetic diversity of Chinese giant salamander (Andrias davidianus) based on the novel microsatellite markers

2012 ◽  
Vol 48 (12) ◽  
pp. 1227-1231 ◽  
Author(s):  
Y. Meng ◽  
Y. Zhang ◽  
H. W. Liang ◽  
H. B. Xiao ◽  
C. X. Xie
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
The Novel ◽  
Andrias Davidianus ◽  
Chinese Giant Salamander

scholarly journals Chinese Giant Salamander (Andrias davidianus) Iridovirus Infection Leads to Apoptotic Cell Death through Mitochondrial Damage, Caspases Activation, and Expression of Apoptotic-Related Genes

2019 ◽  
Vol 20 (24) ◽  
pp. 6149 ◽  
Author(s):  
Yiqun Li ◽  
Nan Jiang ◽  
Yuding Fan ◽  
Yong Zhou ◽  
Wenzhi Liu ◽  
...  
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Mitochondrial Damage ◽  
Apoptotic Cell Death ◽  
Economic Losses ◽  
P53 Expression ◽  
Andrias Davidianus ◽  
Chinese Giant Salamander ◽  
Infected Cells ◽  
Apoptotic Genes

Chinese giant salamander iridovirus (GSIV) is the causative pathogen of Chinese giant salamander (Andrias davidianus) iridovirosis, leading to severe infectious disease and huge economic losses. However, the infection mechanism by GSIV is far from clear. In this study, a Chinese giant salamander muscle (GSM) cell line is used to investigate the mechanism of cell death during GSIV infection. Microscopy observation and DNA ladder analysis revealed that DNA fragmentation happens during GSIV infection. Flow cytometry analysis showed that apoptotic cells in GSIV-infected cells were significantly higher than that in control cells. Caspase 8, 9, and 3 were activated in GSIV-infected cells compared with the uninfected cells. Consistently, mitochondria membrane potential (MMP) was significantly reduced, and cytochrome c was released into cytosol during GSIV infection. p53 expression increased at an early stage of GSIV infection and then slightly decreased late in infection. Furthermore, mRNA expression levels of pro-apoptotic genes participating in the extrinsic and intrinsic pathway were significantly up-regulated during GSIV infection, while those of anti-apoptotic genes were restrained in early infection and then rose in late infection. These results collectively indicate that GSIV induces GSM apoptotic cell death involving mitochondrial damage, caspases activation, p53 expression, and pro-apoptotic molecules up-regulation.

Effects of dietary mulberry leaf extract on the growth, gastrointestinal, hepatic functions of Chinese giant salamander ( Andrias davidianus )

2020 ◽  
Vol 51 (6) ◽  
pp. 2613-2623
Author(s):  
Zhanfu Li ◽  
Xiaochuan Chen ◽  
Yongjun Chen ◽  
Weilong Li ◽  
Qifeng Feng ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Andrias Davidianus ◽  
Mulberry Leaf ◽  
Mulberry Leaf Extract ◽  
Chinese Giant Salamander

The complete mitochondrial genome of the Chinese giant salamander, Andrias davidianus (Amphibia: Caudata)

Gene ◽  
2003 ◽  
Vol 311 ◽  
pp. 93-98 ◽  
Author(s):  
Peng Zhang ◽  
Yue-Qin Chen ◽  
Yi-Fei Liu ◽  
Hui Zhou ◽  
Liang-Hu Qu
Keyword(s):  
Mitochondrial Genome ◽  
Complete Mitochondrial Genome ◽  
Andrias Davidianus ◽  
Chinese Giant Salamander

scholarly journals Comparative study on five kinds of cytokines in the tissues of Chinese giant salamander (Andrias davidianus) of different developmental stages

2021 ◽  
Vol 869 (1) ◽  
pp. 012067
Author(s):  
J Qian ◽  
X Y Zhai ◽  
L Guo ◽  
W G Chen ◽  
J J Fu ◽  
...  
Keyword(s):  
Comparative Study ◽  
Blood Serum ◽  
Developmental Stages ◽  
Distribution Area ◽  
Andrias Davidianus ◽  
Tnf Α ◽  
Sandwich Method ◽  
Different Ages ◽  
Chinese Giant Salamander ◽  
Different Tissues

Abstract By using of the double antibody sandwich method of ELISA, the activities of five cytokines including IL-2, IL-4, IFN-α, IFN-β and TNF-α from the blood serum, liver, intestine and spleen at two developmental stages of Chinese giant salamander (Andrias davidianus) were determined to analyze the distribution of the cytokines. The result indicated that five cytokines were found in these four tissues, while their activities were different in different tissues and different ages. The highest activity of IL-2 and IL-4 was all present in blood serum of two different ages. The activity of IFN-α was the highest in blood serum of 1-year-old and in spleen of 2-year-old, respectively. The activity of IFN-β was also highest in blood serum of two different ages. The activity of TNF-α was highest in liver of two different ages. Thus, this study provides convincing reference for blood serum and liver as the most important distribution area of Chinese giant salamander.

Identification and expression of cytochrome P450 genes in the Chinese giant salamander Andrias davidianus

2017 ◽  
Vol 95 ◽  
pp. 62-68 ◽  
Author(s):  
Qiaomu Hu ◽  
Hanbing Xiao ◽  
Haifeng Tian ◽  
Yan Meng
Keyword(s):  
Cytochrome P450 ◽  
Andrias Davidianus ◽  
Cytochrome P450 Genes ◽  
Chinese Giant Salamander

Pathological and microbiological findings from mortality of the Chinese giant salamander (Andrias davidianus)

2014 ◽  
Vol 159 (6) ◽  
pp. 1403-1412 ◽  
Author(s):  
Yan Meng ◽  
Jie Ma ◽  
Nan Jiang ◽  
Ling-Bing Zeng ◽  
Han-Bing Xiao
Keyword(s):  
Andrias Davidianus ◽  
Chinese Giant Salamander

Ultrastructural Morphogenesis of an Amphibian Iridovirus Isolated from Chinese Giant Salamander (Andrias davidianus)

2014 ◽  
Vol 150 (2-3) ◽  
pp. 325-331 ◽  
Author(s):  
J. Ma ◽  
L. Zeng ◽  
Y. Zhou ◽  
N. Jiang ◽  
H. Zhang ◽  
...  
Keyword(s):  
Andrias Davidianus ◽  
Chinese Giant Salamander
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