Comparison of phenolic components among different species of Dendrobium (Shihu Fengdou) and determination of their active components—moscatilin and gigantol

2010 ◽  
Vol 28 (6) ◽  
pp. 566-571 ◽  
Author(s):  
Jing ZHOU ◽  
Zhiliang XU ◽  
Hongwei KONG ◽  
Xin LU ◽  
Guowang XU
Keyword(s):  
Active Components ◽  
Phenolic Components

Simultaneous Determination of Six Components in Jingzhiguanxin Tablet by High-Performance Liquid Chromatography

2019 ◽  
Vol 15 (2) ◽  
pp. 130-137
Author(s):  
Hui Jiang ◽  
Lianhao Fu ◽  
Yu Wang ◽  
Shaozhi Wang ◽  
Xiaoxu Zhang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Gradient Elution ◽  
Tanshinone Iia ◽  
Array Detector ◽  
Control Analysis ◽  
Active Components ◽  
Quality Control Analysis

Background: Jingzhiguanxin (JZGX) tablet, a traditional Chinese prescription, is commonly used for treating coronary heart disease and angina pectoris in the clinic. There are six active components (Danshensu (DSS), Protocatechuic aldehyde (PD), Paeoniflorin (PF), Ferulic acid (FA), Salvianolic acid B (Sal B) and Tanshinone IIA (TA)) in JZGX tablet. </P><P> Objective: In this paper, a simple and reliable method was used for simultaneous determining the six active components by high-performance liquid chromatography coupled with diode array detector (HPLC-DAD). Methods: These six active components were separated on an Agilent Zorbax Eclipse XDB-C18 column (150 mmx4.6 mm, 5 µm) at 30 °C. Acetonitrile (A), methanol (B) and 0.5% H3PO4 aqueous solution (C) were used as mobile phase for gradient elution. The flow rate was 1 mL/min and the detection wavelengths were set at 280 nm for DSS, PD and Sal B, 230 nm for PF, 320 nm for FA and 270 nm for TA, respectively. Results: All of the six components showed good linearity regressions (r2≥0.9997) in the detected concentration range. The recovery rates and coefficient of variation (CV) for all analytes were 98.66%- 100.18% and 0.75%-1.89%, respectively. This method was successfully applied to simultaneously determine the six components in JZGX tablet from different batches and manufacturers. Conclusion: The validated method can be used in routine quality control analysis of JZGX tablet without any interference.

scholarly journals Exploitation of HPLC Analytical Method for Simultaneous Determination of Six Principal Unsaturated Fatty Acids in Oviductus Ranae Based on Quantitative Analysis of Multi-Components by Single-Marker (QAMS)

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 479
Author(s):  
Shihan Wang ◽  
Yuanshuai Gan ◽  
Hong Kan ◽  
Xinxin Mao ◽  
Yongsheng Wang
Keyword(s):  
Fatty Acids ◽  
Quantitative Analysis ◽  
Unsaturated Fatty Acids ◽  
Internal Standard ◽  
Active Components ◽  
Reliable Determination ◽  
External Standard Method ◽  
Single Marker ◽  
Oviductus Ranae

As one of the featured products in northeast China, Oviductus Ranae has been widely used as a nutritious food, which contains a variety of bioactive unsaturated fatty acids (UFAs). It is necessary to establish a scientific and reliable determination method of UFA contents in Oviductus Ranae. In this work, six principal UFAs in Oviductus Ranae, namely eicosapentaenoic acid (EPA), linolenic acid (ALA), docosahexaenoic acid (DHA), arachidonic acid (ARA), linoleic acid (LA) and oleic acid (OA), were identified using UPLC-MS/MS. The UFAs identified in Oviductus Ranae were further separated based on the optimized RP-HPLC conditions. Quantitative analysis of multi-components by single-marker (QAMS) method was implemented in content determination of EPA, ALA, DHA, ARA and OA, where LA was used as the internal standard. The experiments based on Taguchi design verified the robustness of the QAMS method on different HPLC instruments and chromatographic columns. The QAMS and external standard method (ESM) were used to calculate the UFA content of 15 batches of Oviductus Ranae samples from different regions. The relative error (r < 0.73%) and cosine coefficient showed that the two methods obtained similar contents, and the method validations met the requirements. The results showed that QAMS can comprehensively and effectively control the quality of UFAs in Oviductus Ranae which provides new ideas and solutions for studying the active components in Oviductus Ranae.

scholarly journals Dietary intake of specific phenolic compounds and their effect on the antioxidant activity of daily food rations

2015 ◽  
Vol 13 (1) ◽  
Author(s):  
Wojciech Koch ◽  
Tomasz Baj ◽  
Wirginia Kukula-Koch ◽  
Zbigniew Marzec
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolics ◽  
Female Students ◽  
Male And Female ◽  
Daily Food ◽  
Phenolic Components ◽  
Phenolics Content ◽  
Test Correlation

AbstractThe determination of phenolic acids’ and flavonoids’ content in daily food rations (DFRs) of a selected group of male and female students and the development of chromatographic conditions is the primary goal of the study. The presence of 7 phenolic components were confirmed in the prepared extracts from all diets reconstructed within a period of 3 years. The highest concentrations were determined for hesperidin (124 and 55.6 mg for women and men, respectively), naringin (47.6 mg in female and 37 mg in male diets) and chlorogenic acid (19.7 and 19.8 mg for women and men). The antioxidant potential of the daily food rations, measured with a DPPH test, was higher for women (range 47.1‒78.8%) than for men (range 34.5‒78.0%) and was found to strongly correlate with the total phenolics content of the samples (Folin-Ciocalteu test) (correlation coefficient 0.90).

Improved LC Method for the Simultaneous Determination of Five Active Components in Danshen and Its Preparations

2009 ◽  
Vol 69 (5-6) ◽  
pp. 543-548 ◽  
Author(s):  
Xiao-Bing Li ◽  
Xiao-Mei Xie ◽  
Wei-Zhong Pei ◽  
Jia-Kuan Chen ◽  
Yan Song ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Active Components

Q-marker Prediction Analysis of Rhubarb in Fengyin Decoction Based on Fingerprint and Network Pharmacology

2021 ◽  
Vol 16 (8) ◽  
pp. 1934578X2110387
Author(s):  
Ji-le Lan ◽  
Ye-ping Ruan ◽  
Zhu-jun Mao ◽  
Li-yan You ◽  
Zhong Chen
Keyword(s):  
Chinese Medicine ◽  
High Performance ◽  
Active Component ◽  
Extraction Methods ◽  
Network Pharmacology ◽  
Active Components ◽  
Quality Marker ◽  
Core Components ◽  
Aloe Emodin

Fengyin Decoction (FYD) is a traditional Chinese medicine for the treatment of epilepsy and wind paralysis. However, the potential antiepileptic active component in rhubarb (which is the most effective Chinese medicine in FYD) has not been defined. In this study, we analyzed and predicted the potential quality marker (Q-marker) of rhubarb in FYD based on fingerprint and network pharmacology. The fingerprints of FYD and rhubarb were established to analyze the transmission law of active components. Ultra-high performance liquid chromatography (UPLC) was used to study quantitatively the active components obtained by different extraction methods of FYD. Combined with network pharmacological analysis, a “components-targets-pathways” network was constructed to predict the potential Q-marker. Eight peaks were identified by FYD fingerprint: aloe-emodin, rhein, emodin, chrysophanol, physcion, cinnamaldehyde, 6-gingerol, and glycyrrhizic acid ammonium salt. The determination of the 8 active components in FYD with different extraction methods suggested that rhubarb anthraquinone may be a potential antiepileptic active component. Twelve core components, 19 targets, and 21 pathways of rhubarb were screened by network pharmacology, which further demonstrated that rhubarb played a role mainly through these components, targets, and pathways. We preliminarily predicted that compounds such as rhubarb anthraquinones were a potential Q-marker. The UPLC fingerprint and the content determination method of the 8 components established in this study were effective and feasible. The findings in this study may provide a reference for further study of quality control of FYD and lay a theoretical foundation for the study of its action mechanism. In addition, our study may provide a novel idea for the study of the Q-marker of other classical compound traditional Chinese medicines.

Arrays of solid contact potentiometric sensors for separate determination of some cephalosporin antibiotics

2021 ◽  
Vol 87 (5) ◽  
pp. 5-13
Author(s):  
E. G. Kulapina ◽  
A. E. Dubasova ◽  
O. I. Kulapina ◽  
V. D. Ankina
Keyword(s):  
Response Time ◽  
Reference Model ◽  
Complex Compounds ◽  
Potentiometric Sensors ◽  
Separate Determination ◽  
Solid Contact ◽  
Active Components ◽  
Membrane Surfaces ◽  
Cephalosporin Antibiotics

Arrays of potentiometric sensors including developed solid-contact unmodified and modified sensors based on tetradecylammonium associates with complex compounds of silver (1) and some β-lactam antibiotics (cefazoline, cefuroxime, cefotaxime (n = 3 – 6)) are proposed; polyaniline and copper oxide being modifiers. The main electroanalytic properties of the sensors are determined (the range of the determined concentrations in antibiotic solutions 1 × 10–4 – 0.1 M, 46.3 < S < 48, Cmin = n × 10–5 М, response time 4 – 10 sec, potential drift 4 – 6 mV/day, service life — 2 months). It is shown that modification of the membrane surfaces brings the steepness of the electrode functions to Nernst-values for single-charged ions of the antibiotics under study; reduces the response time and the detection limits, the linearity intervals of the electrode functions being the same. The potentiometric selectivity coefficients of unmodified and modified sensors based on different electrode active components (EAC) to the studied cephalosporins in the presence of interfering antibiotics are close to unity; cross sensitivity parameters for the considered sensors (the average slope of the electrode function of the sensor Sav, the unselectivity factor F, and the reproducibility factor K) are 46.3 < S (mV/pC) < 48; 0.85 < F < 0.90; 144 < K < 170, respectively. Application of sensors in the multisensory analysis of model mixtures of cephalosporin antibiotics is shown. Method of artificial neural networks (ANN) is used for processing of analytical signals. The correctness of the determination is carried out using «spike tests» on the reference model mixtures (the relative error of the determination does not exceed 12 %).

Liquid Chromatographic Determination of Acetaminophen in Multicomponent Analgesic Tablets

1984 ◽  
Vol 67 (2) ◽  
pp. 339-341
Author(s):  
David J Krieger
Keyword(s):  
Chromatographic Determination ◽  
Ultraviolet Region ◽  
Active Components ◽  
Chlorpheniramine Maleate ◽  
Reverse Phase ◽  
Phenylephrine Hydrochloride ◽  
Photometric Detection ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A simple, rapid LC method is presented for the separation and determination of acetaminophen in analgesic preparations containing up to 6 additional active components. The method uses a C18 reverse phase column, methanol–0.75% acetic acid (1 + 3) mobile phase, and photometric detection in the ultraviolet region. Acetaminophen was effectively separated from chlorpheniramine maleate, phenylephrine hydrochloride, caffeine, salicylamide, aspirin, and phenacetin, as well as from salicylic acid, a degradation product of aspirin. Typical chromatograms of the separation of acetaminophen from the above compounds in synthetic mixture and in commercial multicomponent analgesic preparations are presented, along with reproducibility and recovery data.

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