Revascularization and Function of Pancreatic Islet Isografts in Diabetic Rats following Transplantation

2003 ◽  
Vol 12 (5) ◽  
pp. 537-544 ◽  
Author(s):  
Hajime Furuya ◽  
Toshihisa Kimura ◽  
Makoto Murakami ◽  
Kanji Katayama ◽  
Kazuo Hirose ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Diabetic Rats ◽  
Isolated Islets ◽  
Diabetic Rat ◽  
Pancreatic Islet Transplantation ◽  
Intravenous Glucose ◽  
Lewis Rats ◽  
Group A ◽  
Group B ◽  
And Function

In pancreatic islet transplantation, revascularization is crucial for the graft's survival and function. In this study, the endothelium of isolated islets and revascularization and function of islet isografts in diabetic rat were investigated. Islets were isolated from Lewis rats by collagenase digestion method and were examined using immunohistochemistry (CD31 stain) on days 0, 1, 3, and 7 after isolation. The number of CD31-positive cells in these isolated islets was counted (mean ± SD%). Isografts (freshly isolated islets: group A, and islets cultured for 7 days: group B) transplanted in the renal subcapsule of streptozotocin-induced diabetic Lewis rats were examined using immunohistochemistry (CD31 stain) on days 3, 5, and 7 after transplantation. Intravenous glucose tolerance tests (IVGTT) were performed on days 3 and 7 after transplantation. The number of CD31-positive cells in the isolated islets on days 0, 1, 3, and 7 after isolation were: 17.3 ± 4.1%, 8.2 ± 0.7%, 2.1 ± 0.8%, and 0.8 ± 0.5%, respectively (p < 0.05). On day 5 after transplantation, CD31-positive cells were not detected in group A and B grafts, but were detected in both groups in periphery of the islets. On day 7, CD31-positive microvessels were present throughout the entire graft. IVGTT values in groups A and B on days 3 and 7 after transplantation did not show significant differences. In renal subcapsular isografts in diabetic rats, revascularization into islet grafts occurs from the surrounding host tissue 5 days after transplantation, but has no influence on the response to glucose during this period.

scholarly journals Protective effect of LIF-huMSCs on the retina of diabetic model rats

2021 ◽  
Vol 14 (10) ◽  
pp. 1508-1517
Author(s):  
Shan-Na Chen ◽  
◽  
Ying-Xue Ma ◽  
Song Chen ◽  
Guang-Hui He ◽  
...  
Keyword(s):  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Model ◽  
Group A ◽  
Retinal Structure ◽  
Hs Crp ◽  
Group B ◽  
And Function ◽  
Group D ◽  
Control Group Group

AIM: To investigate the protective effect of human umbilical cord mesenchymal stem cells (hUCMSCs) modified by the LIF gene on the retinal function of diabetic model rats and preliminarily explore the possible mechanism. METHODS: A stably transfected cell line of hUCMSCs overexpressing leukemia inhibitory factor (LIF) was constructed. Overexpression was verified by fluorescent quantitative polymerase chain reaction (qPCR). Forty-eight adult Sprague-Dawley rats were randomly divided into a normal control group (group A), streptozotocin-induced diabetic control group (group B), diabetic rats at 3mo injected with empty vector-transfected hUCMSCs (group C) or injected with LIF-hUCMSCs (group D). Four weeks after the intravitreal injection, analyses in all groups included retinal function using flash electroretinogram (F-ERG), retinal blood vessel examination of retinal flat mounts perfused with fluorescein isothiocyanate-dextran (FITC-dextran), and retinal structure examination of sections using hematoxylin and eosin staining. Expression levels of adiponectin (APN), high-sensitivity C-reactive protein (hs-CRP), and neurotrophin-4 (NT-4) in each group was detected using immunohistochemistry, PCR, Western blotting, and ELISA, respectively. RESULTS: A stable transgenic cell line of LIF-hUCMSCs was constructed. F-ERG and FITC-dextran examinations revealed no abnormalities of retinal structure and function in group A, severe damage of the retinal blood vessels and function in group B, and improved retinal structure and function in group C and especially group D. qPCR, ELISA, and Western blot analyses revealed progressively higher APN and NT-4 expression levels in groups B, C, and D than in group A. hs-CRP expression was significantly higher in group B than in groups A, C, and D, and was significantly higher in group C than in group D (P<0.05). CONCLUSION: LIF-hUCMSCs protect the retina of diabetic rats by upregulating APN and NT-4 expression and downregulating hs-CRP expression in the retina.

scholarly journals Ekstrak Etanol Daun Kacang Panjang (Vigna unguiculata (L.) Walp): Kajian Morfometri Insula Pankreatika Model Tikus Diabetes

2019 ◽  
Vol 5 (2) ◽  
pp. 183
Author(s):  
Puput Octaviani Solehah ◽  
Fikhta Agnesya Tarusu ◽  
Joni Tandi ◽  
Niluh Puspita Dewi ◽  
Gabriella Bamba Ratih Lintin ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Vigna Unguiculata ◽  
Therapy Group ◽  
Statistical Tests ◽  
Ethanol Extract ◽  
Diabetic Rat ◽  
Control Group ◽  
Blood Levels ◽  
Group A ◽  
Group B

Diabetes is characterized by an increasing of blood levels and progressive changes in the structure of pancreatic islet of pancreas. Natural ingredients that contain antioxidants such as some cowpea leaves can be an alternative treatment for diabetes. This study aims to examine the effect of ethanol extract of some cowepea leaves (Vigna unguiculata (L.) Walp) on morphometry (perimeter and diameter) of pancreatic islet of diabetic rat models. The type of research is quasi-experimental with posttest group design. Samples were 30 male wistar rats, age 8 weeks, BW 250-300 grams, that induced by single dose of STZ 40 mg/kg BW. Rats were divided into 5 groups. Group A: normal control, Group B: negative control, Group C: positive control, Group D: DM rats with 100mg/Kg BW extract therapy, Group E: DM rats with 200mg/Kg BW extract therapy, Group F: DM rats with extract therapy 300mg/Kg BW. Blood sugar levels were measured on days 7, 14 and 21. Pancreatic tissue was prepared with 5μm thickness and stained with Hematoxylin Eosin. The sample was observed under 400x magnification microscope. The observation uses Image J software to measure the perimeter and diameter of the pancreatic insula. Data were analyzed by non-parametric Kruskal Wallis and post hoct Mann Whitney statistical tests. Blood glucose level was obtained on 21st day with the highest mean in group B (246.8±156.3) and lowest in group A (67.2 ±7.85), perimeter of pancreatic islet was significantly different (p=0.026), the diameter is significantly different (p =0.046). Ethanol extract of some cowpea (Vigna unguiculata (L.) Walp) leaves with dose of 300mg/kgBB helps to restore the blood and morphometry of pancreatic islet model of diabetic rats.

scholarly journals Ekstrak Etanol Daun Kacang Panjang (Vigna unguiculata (L.) Walp): Kajian Morfometri Insula Pankreatika Model Tikus Diabetes

2019 ◽  
Vol 5 (2) ◽  
pp. 183-188
Author(s):  
Puput Octaviani Solehah ◽  
Fikhta Agnesya Tarusu ◽  
Joni Tandi ◽  
Niluh Puspita Dewi ◽  
Gabriella Bamba Ratih Lintin ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Vigna Unguiculata ◽  
Therapy Group ◽  
Statistical Tests ◽  
Ethanol Extract ◽  
Diabetic Rat ◽  
Control Group ◽  
Blood Levels ◽  
Group A ◽  
Group B

Diabetes is characterized by an increasing of blood levels and progressive changes in the structure of pancreatic islet of pancreas. Natural ingredients that contain antioxidants such as some cowpea leaves can be an alternative treatment for diabetes. This study aims to examine the effect of ethanol extract of some cowepea leaves (Vigna unguiculata (L.) Walp) on morphometry (perimeter and diameter) of pancreatic islet of diabetic rat models. The type of research is quasi-experimental with posttest group design. Samples were 30 male wistar rats, age 8 weeks, BW 250-300 grams, that induced by single dose of STZ 40 mg/kg BW. Rats were divided into 5 groups. Group A: normal control, Group B: negative control, Group C: positive control, Group D: DM rats with 100mg/Kg BW extract therapy, Group E: DM rats with 200mg/Kg BW extract therapy, Group F: DM rats with extract therapy 300mg/Kg BW. Blood sugar levels were measured on days 7, 14 and 21. Pancreatic tissue was prepared with 5μm thickness and stained with Hematoxylin Eosin. The sample was observed under 400x magnification microscope. The observation uses Image J software to measure the perimeter and diameter of the pancreatic insula. Data were analyzed by non-parametric Kruskal Wallis and post hoct Mann Whitney statistical tests. Blood glucose level was obtained on 21st day with the highest mean in group B (246.8±156.3) and lowest in group A (67.2 ±7.85), perimeter of pancreatic islet was significantly different (p=0.026), the diameter is significantly different (p =0.046). Ethanol extract of some cowpea (Vigna unguiculata (L.) Walp) leaves with dose of 300mg/kgBB helps to restore the blood and morphometry of pancreatic islet model of diabetic rats.

REVASCULARIZATION AND FUNCTION OF PANCREATIC ISLET ISOGRAFTS IN DIABETIC RATS FOLLOWING TRANSPLANTATION.

2004 ◽  
Vol 78 ◽  
pp. 622-623
Author(s):  
H Furuya ◽  
T Kimura ◽  
M Morikawa ◽  
M Murakami ◽  
K Katayama ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Diabetic Rats ◽  
And Function

Peripheral, but not Central, Nervous System Abnormalities are Reversed by Pancreatic Islet Transplantation in Diabetic Lewis Rats

1996 ◽  
Vol 8 (6) ◽  
pp. 1117-1123 ◽  
Author(s):  
S. Morano ◽  
M. Sensi ◽  
S. Gregorio ◽  
G. Pozzessere ◽  
A. F. G. Petrucci ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Islet Transplantation ◽  
Pancreatic Islet ◽  
Pancreatic Islet Transplantation ◽  
Lewis Rats

scholarly journals Surgical techniques for the treatment of tongue tie in children: a comparative study

2016 ◽  
Vol 2 (3) ◽  
pp. 128
Author(s):  
Atishkumar Gujrathi ◽  
Vijayalaxmi Ambulgekar ◽  
Ashwini Handal
Keyword(s):  
Surgical Techniques ◽  
Female Child ◽  
Social Effects ◽  
Age Group ◽  
Class Iii ◽  
Medical College ◽  
Group A ◽  
Surgical Modality ◽  
Group B ◽  
And Function

<p class="abstract"><strong>Background:</strong> Ankyloglossia is another name for tongue tie which in mild form is characterized by mucous membrane bands to complete ankyloglossia whereby the tongue is tethered to the floor of the mouth. It can affect feeding, speech, and oral hygiene as well as have mechanical/social effects. Ankyloglossia can also prevent the tongue from contacting the anterior palate.</p><p class="abstract"><strong>Methods:</strong> The study aimed to find out best possible surgical modality of frenectomy by comparing scalpel, electro-cautery and CO<sub>2</sub> laser in the treatment of tongue tie. This is a prospective randomized clinical trial conducted in the department of ENT, Dr. Shankarrao Chavan Government Medical College, Nanded, Maharashtra. All patients were categorized in to three groups randomly as group A, group B and group C. Each group contains 18 patients and among group A, B and C, frenectomy was done by conventional scalpel technique, by bipolar cautery and CO<sub>2</sub> laser respectively. Then patients were assessed on post op day 1 for symptomatology and inflammatory signs, on post op day 7 for wound healing and any complications and also after 1 month post-op for scar and contracture of wound.  </p><p class="abstract"><strong>Results:</strong> In our study, about 61% of population is of male child and female child were remaining 39% (ratio 1.6:1) which is matching with the previous studies. Amongst all patients most common age group is between 1-4 years of age group. Most of the patients were in Kotlow’s class III having severe ankyloglossia (3‑7 mm) followed by class I having Mild ankyloglossia (12‑16 mm).</p><p><strong>Conclusions:</strong> Laser and electro-cautery treatment used for frenectomy operations provides better patient perception in terms of postoperative pain and function than that obtained by the scalpel technique.</p>

scholarly journals Assessment of Different Oxidative, Endocrinological and Biochemical Parameters using Catharanthus Roseus on Rats

2021 ◽  
pp. 312-317
Author(s):  
Eshwa Dar ◽  
Abdul Rashid Shaikh ◽  
Muhammad Shahbaz Yousaf ◽  
Muhammad Adnan ◽  
Samar Akhtar ◽  
...  
Keyword(s):  
Catharanthus Roseus ◽  
Biochemical Parameters ◽  
Basal Diet ◽  
Negative Control ◽  
Diabetic Rats ◽  
Diet Group ◽  
Herbal Extract ◽  
Stress Relieving ◽  
Group A ◽  
Group B

The current study was planned to assess the different oxidative, Endocrinological and biochemical parameters using extract of Catharanthus roseus (C. roseus) on diabetic rats. Three groups of Diabetic rats were assigned viz. Group A have Negative Control which was fed basal diet; Group B have Positive Control and was non-supplemented; and Group C supplemented with C. roseus extract. The results showed that blood glucose level, ALT, TGs and urea concentration was elevated non-significantly (P>0.05), of Group C which was supplemented by C. roseus when compared with non-supplemented Group B animals. In Group C which was supplemented by C. roseus, AST, and Cholesterol results were decreased as showed by statistics analysis (non-significantly). When level of Blood cortisol was compared between Group C and B, there was increased in Group C, as per non-significant statistics measurements (P>0.05). It was observed that level of T4 and T3 hormones was decreased in Group C (supplemented by herbal extract) when compared with Group B. When catalase enzyme results were checked in animals comprised of Group A, B and C, it showed that rate of catalase increased, respectively. In conclusion, Catharanthus roseus restore the levels of thyroid hormones, and cholesterol; and elevate catalase enzyme activity in animals which produce stress relieving effect.

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