Comparative SEM Study on the Effect of Irrigating the Radicular Dentine with NaOCl and EDTA Through Conventional Techniques and Diode Laser

2017 ◽  
Vol 68 (5) ◽  
pp. 916-920
Author(s):  
Alexandra Onac ◽  
Anamaria Florescu ◽  
Andreea Dana Tudose ◽  
Stefan Manea ◽  
Anna Maria Pangica ◽  
...  
Keyword(s):  
Diode Laser ◽  
Laser Diode ◽  
Water Solution ◽  
Conventional Technique ◽  
Sem Analysis ◽  
Smear Layer ◽  
Single Root ◽  
Sem Study ◽  
Group A ◽  
Group B

Since there are not many references in literature about the action of diode laser 940nm on the dentin and root smear layer, the aim of this study is to evaluate and compare, through SEM analysis, the efficiency of the Sodium hypochlorite 2% (CERKAMED) - EDTA 17% (CERKAMED) combination, operated in the middle and apical third of the root, by conventional syringe needle EndoEze and diode laser 940nm treatment. The study was performed on 40 single-root teeth, which were divided into two groups: group A consisting of 20 teeth, for which irrigation was performed only with the syringe and a top open side needle EndoEze Irrigator, resulting in a turbulence moving upward which efficiently irrigates the root canal and prevents periapical water solution; and group B consisting of 20 teeth, which benefited from irrigation associated with diode laser 940nm treatment. In the middle third and apical third smear layer removal was more effectively achieved when teeth benefited from irrigation associated with the laser diode treatment. In Endodontics, Diode laser is a good decontamination system, with biostimulation effect, which is important in healing the periapicale area. Significantly better results were obtained using 940nm laser diode associated with successive irrigation with 2% NaOCl and 17% EDTA, as compared to the conventional technique performed by syringe and lavage needle EndoEze Irrigator (Ultradent).

scholarly journals The Effect of Different Cleaning Protocols on Post Space: A SEM Study

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Giuseppe Lo Giudice ◽  
Angelo Lizio ◽  
Roberto Lo Giudice ◽  
Antonio Centofanti ◽  
Giuseppina Rizzo ◽  
...  
Keyword(s):  
Orthophosphoric Acid ◽  
Acid Group ◽  
Smear Layer ◽  
Control Group ◽  
Apical Direction ◽  
Sem Study ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Post Space

Aim. Purpose of the present paper is to analyze the efficiency of different post-space irrigation protocols.Methods. 28 single rooted teeth were endodontically treated. After post-space preparation every sample was assigned to one of three experimental groups and to one control group. In each group different irrigation protocols were performed as follows: EDTA (Group A), 37% orthophosphoric acid (Group B), and EDTA + 37% orthophosphoric acid with ultrasounds activation (Group C). In the control group (Group D) the irrigate association was not activated by ultrasounds. Three zones (coronal, middle, and apical) of each sample were analyzed by using Scan Electron Microscopy (SEM) without any metallization procedures. The presence of smear layer on the canal surface was qualitatively evaluated by applying Serafino’s score with values included between 0 and 2.Results. The results of the research showed how Group C recorded the better results (0.81±0.72). Group A and Group B showed lower mean scores (1.06±0.69and1.08±0.77); Group D showed the lowest mean score of1.30±0.69. The SEM observation analysis demonstrated how the smear layer presence decreased in the crown-apical direction.Conclusions. The different post-space treatments statistically determine significant differences on the dentinal surfaces cleansing. The absence of ultrasonic activation lowers the cleansing efficacy of endocanalar irrigants, showing sensible differences among each post-space zone.

scholarly journals Evaluation of Effectiveness of Cleaning of Root Canals using Protaper and K3 Rotary Systems: A SEM Study

2015 ◽  
Vol 6 (1) ◽  
pp. 20-25
Author(s):  
K Shashikala ◽  
BS Keshava Prasad ◽  
Anukriti Tyagi
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Smear Layer ◽  
Root Canals ◽  
Significant Difference ◽  
Sem Study ◽  
Group A ◽  
Group B ◽  
Scanning Electron

ABSTRACT The aim of this in vitro study was to compare the debris and smear layer removal following root canal preparation using two different rotary systems with scanning electron microscope (SEM). The rotary systems used were Protaper and K3. Forty single rooted permanent mandibular premolars were chosen for the study. They were assigned two groups on the basis of instrumentation used. The teeth were sectioned at the level of cementoenamel junction and instrumented with Protaper in group A and with K3 in group B. The root canals were thoroughly irrigated with 5 ml of 2.5 % NaOCl during instrumentation. After instrumentation, 5 ml of normal saline was used as a final rinse. The teeth were split longitudinally and the specimens were prepared for SEM evaluation. Scanning electron microscope photomicrographs showed presence of debris and smear layer. The SEM photomicrographs were scored, based on the standard score rating system, and the scores were tabulated accordingly. The scores obtained from the specimens were subjected to statistical analysis. Results showed opening of dentinal tubules and effective removal of smear layer in group A (Protaper) and no significant difference between both the groups (groups A and B) regarding debris. How to cite this article Tyagi A, Prasad BSK, Shashikala K. Evaluation of Effectiveness of Cleaning of Root Canals using Protaper and K3 Rotary Systems: A Sem Study. World J Dent 2015;6(1):20-25.

scholarly journals Laser versus LED polymerization: Comparison of in vitro preventive sealing procedures

2019 ◽  
Vol 17 (1) ◽  
pp. 228080001881601
Author(s):  
Filiberto Mastrangelo ◽  
Isabella Perraro ◽  
Sabrina Mattia ◽  
Giuseppe Troiano ◽  
Khrystyna Zhurakivska ◽  
...  
Keyword(s):  
Laser Diode ◽  
Tooth Enamel ◽  
In Vitro Study ◽  
Third Molars ◽  
Fissure Sealant ◽  
Microscopic Evaluation ◽  
Group A ◽  
Group B ◽  
Metallization Process

Introduction: The technique of sealing is a widely accepted procedure for prevention of caries. The aim of our in vitro study was to compare the effect of two different curing units (traditional LED source and innovative laser diode lamp) on the integrity of fissure sealant material and its interface with tooth enamel. Materials and methods: Sixty healthy third molars were randomly assigned to two groups. In group A were teeth intended for polymerization by LED B lamp, and group B comprised teeth to be polymerized by an innovative laser diode. Both groups were treated with the traditional sealing technique, subjected to a metallization process, and analyzed by scanning electron microscope. Results: Micro-gaps between the sealant and the enamel were found in specimens in both A (43%) and B (40%) groups ( p=0.793), and sealant shrinkage was seen. Significant differences between the groups emerged in the percentage of perimetric micro-erosion sites (80% vs. 100%, p=0.010) and the presence of holes and micro-bubbles on the sealant surface (21% vs. 63%, p=0.001). Conclusions: Although macroscopic clinical polymerization occurred with both instruments, the microscopic evaluation showed significant differences between the studied groups in terms of perimetric micro-erosion sites and micro-bubbles, which were higher in laser-cured samples.

scholarly journals Dentin tubule obturation of a bioglass-based dentin desensitizer under repeated exposure to lactid acid and brushing

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Andrea S. Manz ◽  
Thomas Attin ◽  
Beatrice Sener ◽  
Philipp Sahrmann
Keyword(s):  
Lactic Acid ◽  
Surface Composition ◽  
Superficial Layer ◽  
Sem Analysis ◽  
Collagen Fibers ◽  
Dentin Hypersensitivity ◽  
Edx Analysis ◽  
Dentin Tubule ◽  
Group A ◽  
Group B

Abstract Background Dentin hypersensitivity is a frequent finding especially in periodontitis patients. Conventional treatment aims for obstruction of dentin tubules by disabling liquid and osmotic fluctuation to and from the pulpal chamber. A novel bioglass-based desensitizer was shown to obstruct tubules and to resist periodic exposure to lactic acid. Whether this obstruction is resistant to brushing had not been tested so far. Accordingly, the present study aimed to assess dentin tubule obstruction after repeated acid exposure and brushing. Methods Sixty dentin discs were cleaned with 17% EDTA, mounted into a pulp fluid simulator and randomly divided into 3 groups: No surface treatment in Group A, Seal&Protect® in group B and DentinoCer in group C. Discs were exposed to 0.1 M non-saturated lactic acid thrice and standardized brushing twice a day for 12 days. At baseline and after 2, 4 and 12 d samples were removed from the setting and prepared for top-view SEM analysis to assess tubule obstruction using the Olley score. Discs were then vertically cut and the section surface morphologically assessed using backscatter imaging. For both vertical and sectional surfaces EDX analysis was used to characterize the surface composition in the tubular and inter-tubular area. Results Group A showed clean tubular lumina at all time points. From day 2 onwards dentin showed exposed collagen fibers. Group 2 initially showed a complete surface coverage that flattened out during treatment without ever exposing tubules. At baseline, samples of Group C displayed a complete homogeneous coverage. From day 2 on tubules entrances with obstructed lumen became visible. While on day 4 and 12 the dentin surface exposed collagen fibers the lumina remained closed. EDX analysis of the vertical and horizontal views showed that P and Ca were predominant elements in both the inter- and tubular dentin while Si peaks were found in the tubule plugs. Conclusion While group B displayed a packed layer on the surface during the whole investigation time group C samples lost their superficial layer within 48 h. Tubule plugs containing considerable Si proportions indicated previous presence of DentinoCer, while high Ca and P proportions suggest obturation by dentin-like material.

scholarly journals Pull-Type Radiologically Inserted Gastrostomy: An Improvised Technique Using a Frugal Innovation

2019 ◽  
Vol 03 (01) ◽  
pp. 007-011
Author(s):  
Anurag Chahal ◽  
Sundeep Malla ◽  
Chinmaya Dash ◽  
Deepak Gupta ◽  
Shivanand Gamanagatti
Keyword(s):  
Radiation Dose ◽  
Safety Profile ◽  
Conventional Technique ◽  
New Technique ◽  
Fluoroscopy Time ◽  
P Value ◽  
Complication Rates ◽  
Nasogastric Feeding ◽  
Group A ◽  
Group B

Abstract Purpose To describe a new technique for “pull type” radiologically inserted gastrostomy (RIG) and prospectively compare it with conventional (described) technique in terms of technical ease (fluoroscopy time, radiation dose) and safety profile. Materials and Methods Adult patients with head injury with Glasgow coma scale (GCS) (M score) < 5, one week after decompression or those requiring nutritional support for > 4 weeks, or patients with recurrent aspiration pneumonitis on nasogastric feeding were included. Hemodynamically unstable patients or those with uncorrectable coagulopathy were excluded. Patients underwent pull-type RIG with alternate patient getting allocated to groups A (conventional technique) and B (new technique). The authors compared the technical success, complication rate, fluoroscopy time, radiation dose, and cost of hardware in these two groups. Results The average fluoroscopy time for group A (9 patients) was 498.7 ± 145.3 seconds, whereas for group B (16 patients), it was 302.8 ± 54.1 seconds with p-value = 0.06. The mean radiation dose of group A was 74.7 ± 15.7 mGy, whereas for group B, it was 56.7 ± 14.1 mGy (p-value = 0.004). The minor complication rates were 11.1% in both the groups. Conclusion The authors’ innovative technique using a customized snare has the potential to increase the technical ease of pull-type RIG with reduced fluoroscopy time, radiation dose, and cost with a similar safety profile.

Microleakage and SEM Analysis of Flowable Resin Used as a Sealant Following Three Fissure Preparation Techniques – An in vitro Study

2011 ◽  
Vol 35 (3) ◽  
pp. 277-282 ◽  
Author(s):  
TR Chaitra ◽  
VV Subba Reddy ◽  
GM Devarasa ◽  
TL Ravishankar
Keyword(s):  
Statistical Significance ◽  
In Vitro Study ◽  
Sem Analysis ◽  
Vitro Study ◽  
Blue Dye ◽  
Sem Images ◽  
Group A ◽  
Excellent Method ◽  
Group B

Objective: Preventive procedures using pit and fissure sealants are one of the important aspects of pediatric dental practice. The objectives of this in vitro study were to comparatively evaluate microleakage and resin tag penetration of a flowable resin used as a sealant on molars after preparation with Conventional, Enameloplasty and Fissurotomy techniques. Method: A total of 48 teeth were divided into 3 groups (n-16). Group A(CST),Group B (EST),and Group C (FT). Following the fissure preparation sealant was applied as per manufacturer's instructions. 8 samples in each group were used for Microleakage and Resin tag analysis. Microleakage analysis was done under Stereomicroscope after Methylene blue dye immersion by using scoring system. The resin tag analysis was done by measuring the length of resin tags on scanning electron microscope (SEM) images consisting of 10μm scale taken under optical zoom of 2000 X. Results: Significantly lesser microleakage score (P&lt;0.01) and longer resin tag penetration (P&lt;0.001) observed in EST(mean score 0.5±0.53) & (12.19±1.93μm) when compared to CST(mean score 1.75±0.89) & (5.96±1.84μm)and FT (mean score 1.5±0.53) & (6.76±1.82μm) which showed more microleakage and short resin tags. No statistical significance between CST and FT was found. Conclusion: EST has proven to be an excellent method for preparation of pits and fissures when Flowable composite is used as a sealant because of its lesser microleakage and longer resin tag penetration.

scholarly journals Endodontic Irrigants and Their Activation Efficacy on Cleansing Post-Space Root Canal Walls

Prosthesis ◽  
2021 ◽  
Vol 3 (4) ◽  
pp. 406-414
Author(s):  
Manuela Marchese ◽  
Pontoriero I. K. Denise ◽  
Edoardo Ferrari Cagidiaco ◽  
Alfredo Iandolo ◽  
Simone Grandini ◽  
...  
Keyword(s):  
Root Canal ◽  
Continuous Wave ◽  
Smear Layer ◽  
The Other ◽  
X Rays ◽  
Ultrasonic Activation ◽  
Gutta Percha ◽  
Bonding System ◽  
Group A ◽  
Group B

The aim of this study was to evaluate the efficacy of activated irrigants (EDTA e NaOCL) during the cleansing of root walls, of the smear layer, of the debris, and gutta-percha after the preparation of the restorative space. Twenty single and multi-rooted (n = 20) have been collected. All samples were prepared by the same operator, using Nickel-titanium rotating instruments (Mtwo) through the Simultaneous Shaping Technique. The continuous-wave of condensation technique of obturation was used. To all specimens, the restorative space has been made, leaving 5 mm of apical gutta-percha, and postoperative periapical X-rays were performed. The samples were randomly divided into two groups: Group (A): cleansing of the root walls with ultrasonic activation of the irrigants (NEWTRON P5 XS; Satelec Acteon); Group (B): radicular walls wash without ultrasonic activation of endodontic irrigants (NaOCl 5.25% and EDTA 17%). Both dental sample groups were cut longitudinally with a low-speed saw (Isomet); the samples were observed by using a scanning electron microscope (Jeol, Jsm-6060LV) in order to evaluate: (1) the amount of debris/smear layer; (2) the mount of obstruction of dentinal tubules found in the two groups; and (3) evaluation of the presence of gutta-percha. Then, the other five samples each group (with and without ultrasonic activation) were prepared following the same protocol. Then, a universal bonding system (G-Praemio Bond, GC) and a layer of a flowable resin composite (Gaenial Flow, GC) were light-cured and used on top of the prepared root canal walls. The samples were cut in two pieces along the long axis of the root. Then, half sample teeth were kept in an acidic solution (37% HCl) for 48 h in order to completely dissolve dental structures and to have a direct view of resin tags formation under SEM. The other half was prepared to observe the adhesive interface under SEM. The amount of debris was not satisfactory in 9 out of 10 cases in Group B, while in Group A, which has been treated with ultrasounds, the result was either good or great in most of the samples. For the sample group treated with ultrasound, the tubules were evaluated as perfectly clean in 9 out of 10 cases, instead, the results are unsatisfactory for 9 out of 10 cases of group B not treated with ultrasound. Differences between Group A and B were statistically significant. With respect to the presence of debris and tubules obstruction treatment with ultrasonic activation, it offers with no doubt better results. When ultrasonic activation is used in combination with endodontic irrigants, a clean dentin substrate is be obtained for the adhesion of restorative materials, but in order to confirm the findings of this study, further in vivo trials are needed.

scholarly journals HEMORRHOIDECTOMY;

2017 ◽  
Vol 24 (09) ◽  
pp. 1316-1321
Author(s):  
Shibber Ahmed ◽  
Ishtiaq Ahmad ◽  
Humayun Amjid ◽  
Aamir Furqan
Keyword(s):  
Hospital Stay ◽  
Severe Pain ◽  
Conventional Technique ◽  
Categorical Variables ◽  
P Value ◽  
Chi Square ◽  
Post Operative Pain ◽  
Group A ◽  
And Gender ◽  
Group B

Objectives: Is to compare outcomes in terms of mean post-operative pain andhospital stay between stapled versus conventional hemorrhoidectomy. Hypothesis: There isa difference in mean post-operative pain and hospital stay between stapled and conventionalhemorrhoidectomy, stapled technique is better than conventional technique. Study Design:Randomized control trial. Setting: Department of General Surgery Bakhtawar Amin MemorialHospital Multan. Period: February 2016 February 2017. Methodology: A total number of 60patients enrolled in the study, both genders. Statistical software SPSS ver.23 was used toanalyze the data. Mean and SD were calculated and presented for numerical variables likeduration of hemorrhoids, age and pain score while frequencies and percentage were calculatedand presented for categorical variables like ender and grade of hemorrhoids. Independent t-testand chi square test were applied to see effect modification. P value ≤0.05 was considered assignificant. Results: Total number of 60 (100%) patients in the study, 32 (53.3%) were maleand 28 (46.7 %) were female. Mean hospital stay in group A was 1.63 ± 0.71 and in groupB means duration of hospital stay was 1.73 ± 0.74. A P value was 0.001. On stratification ofdata it is concluded that in group A 9 patients have no pain 6 have mild and 6 have moderatepain and 5 patients have severe pain, similarly in group B, 3 patients have no pain 2 have mildpain 1 have moderate pain and no patient have severe pain. P value for male patients was2.65. Conclusion: This study confirms that stapled hemorrhoidectomy is associated with lesspostoperative pain with no effect of age and gender on outcome.

Evaluation of the Smear Layer Removal Effectiveness of EDTA Using Two Techniques: An SEM Study

2006 ◽  
Vol 7 (1) ◽  
pp. 9-16 ◽  
Author(s):  
A. Cemal Tinaz ◽  
Tayfun Alaçam ◽  
L. Sibel Karadag ◽  
Tansev Mihçioğlu
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Smear Layer ◽  
Control Group ◽  
Electron Microscopic ◽  
Human Teeth ◽  
Layer Removal ◽  
Single Root ◽  
Sem Study

Abstract Obtaining the cleanest canal possible before obturation is one of the goals of endodontic treatment. The purpose of this study was to compare the smear layer removal capability of ethylenediamine tetra-acetic acid (EDTA) application with passive ultrasonic and cotton wrapped on reamer activation. Twelve extracted human teeth with single root canals were used for the study. They were conventionally hand instrumented using reamers and K files. The apical aspect of the canals was enlarged to a #40 file. The teeth were divided into 4 four groups, instrumented and irrigated as follows: Group-A EDTA agitated at the end of preparation with cotton wrapped on a reamer for 1 min; Group-B EDTA applied with ultrasonic agitation for 1 min; Group-C irrigated with EDTA+sodium hypochlorite (NaOCl) (negative control group); and Group-D irrigated with distilled water (positive control group). After scanning electron microscopic study at three different levels, smear layer and dentinal tubules were scored. Means were tested for significance using the Z test. When the techniques were compared, the cotton wrapped on reamer agitation method was as successful as the ultrasonic activation of the files. Although all groups had significantly higher smear layer scores at apical compared to coronal sections, no significant differences were recorded. Citation Tinaz AC, Karadag LS, Alaçam T, Mihçioglu T. Evaluation of the Smear Layer Removal Effectiveness of EDTA Using Two Techniques: An SEM Study. J Contemp Dent Pract 2006 February;(7)1:009-016.

scholarly journals Effectiveness of a Canal Brush on Removing Smear Layer: A Scanning Electron Microscopic Study

2013 ◽  
Vol 24 (6) ◽  
pp. 580-584 ◽  
Author(s):  
Eleni Protogerou ◽  
Ioanna Arvaniti ◽  
Ioannis Vlachos ◽  
Marouan Georgios Khabbaz
Keyword(s):  
Statistical Difference ◽  
Optical Microscope ◽  
Smear Layer ◽  
Electron Microscopic ◽  
Root Canals ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Group A ◽  
Group B ◽  
Scanning Electron

This study evaluated the effectiveness of a highly flexible endodontic brush made of polypropylene canal brush (CanalBrush; Coltène) on smear layer removal from the canal walls when used according to the manufacturer's recommendations. Forty-four single-rooted mandibular incisors were prepared to apical size 30/0.06 and randomly divided into three groups A, B and C, where the final irrigation regimen was 10 mL 17% EDTA and 10 mL 2.5% NaOCl for group A, 10 mL EDTA, 5 mL NaOCl, CanalBrush for 20 s at 450 rpm and 5 mL NaOCl for group B, 10 mL NaOCl, CanalBrush and 10 mL NaOCl for group C. One medium-sized CanalBrush was used for each root canal and all brushes were examined under the optical microscope after application to evaluate bristle deformation. Afterwards, roots were split longitudinally and the presence of smear layer was evaluated under a scanning electron microscope. Used brushes invariably exhibited bristle deformation. Group C exhibited the highest means of smear layer in all thirds. Comparing the apical thirds in all groups, there was no statistical difference between groups A and B (3.64±0.48 and 3.68±0.62 respectively), while group C exhibited significantly higher scores (3.9±0.28) than the other two groups. In conclusion, the CanalBrush proved unable to remove smear layer from the instrumented root canals, when used according to the manufacturers' instructions.

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