scholarly journals Dentin tubule obturation of a bioglass-based dentin desensitizer under repeated exposure to lactid acid and brushing

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Andrea S. Manz ◽  
Thomas Attin ◽  
Beatrice Sener ◽  
Philipp Sahrmann
Keyword(s):  
Lactic Acid ◽  
Surface Composition ◽  
Superficial Layer ◽  
Sem Analysis ◽  
Collagen Fibers ◽  
Dentin Hypersensitivity ◽  
Edx Analysis ◽  
Dentin Tubule ◽  
Group A ◽  
Group B

Abstract Background Dentin hypersensitivity is a frequent finding especially in periodontitis patients. Conventional treatment aims for obstruction of dentin tubules by disabling liquid and osmotic fluctuation to and from the pulpal chamber. A novel bioglass-based desensitizer was shown to obstruct tubules and to resist periodic exposure to lactic acid. Whether this obstruction is resistant to brushing had not been tested so far. Accordingly, the present study aimed to assess dentin tubule obstruction after repeated acid exposure and brushing. Methods Sixty dentin discs were cleaned with 17% EDTA, mounted into a pulp fluid simulator and randomly divided into 3 groups: No surface treatment in Group A, Seal&Protect® in group B and DentinoCer in group C. Discs were exposed to 0.1 M non-saturated lactic acid thrice and standardized brushing twice a day for 12 days. At baseline and after 2, 4 and 12 d samples were removed from the setting and prepared for top-view SEM analysis to assess tubule obstruction using the Olley score. Discs were then vertically cut and the section surface morphologically assessed using backscatter imaging. For both vertical and sectional surfaces EDX analysis was used to characterize the surface composition in the tubular and inter-tubular area. Results Group A showed clean tubular lumina at all time points. From day 2 onwards dentin showed exposed collagen fibers. Group 2 initially showed a complete surface coverage that flattened out during treatment without ever exposing tubules. At baseline, samples of Group C displayed a complete homogeneous coverage. From day 2 on tubules entrances with obstructed lumen became visible. While on day 4 and 12 the dentin surface exposed collagen fibers the lumina remained closed. EDX analysis of the vertical and horizontal views showed that P and Ca were predominant elements in both the inter- and tubular dentin while Si peaks were found in the tubule plugs. Conclusion While group B displayed a packed layer on the surface during the whole investigation time group C samples lost their superficial layer within 48 h. Tubule plugs containing considerable Si proportions indicated previous presence of DentinoCer, while high Ca and P proportions suggest obturation by dentin-like material.

scholarly journals A Comparative Study of Bioartificial Bone Tissue Poly-L-lactic Acid/Polycaprolactone and PLLA Scaffolds Applied in Bone Regeneration

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Weizong Weng ◽  
Shaojun Song ◽  
Liehu Cao ◽  
Xiao Chen ◽  
Yuanqi Cai ◽  
...  
Keyword(s):  
Lactic Acid ◽  
New Zealand ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Defect ◽  
Mineral Density ◽  
Group A ◽  
Repair Bone Defect ◽  
Group B ◽  
New Zealand Rabbits

Bioartificial bone tissue engineering is an increasingly popular technique to repair bone defect caused by injury or disease. This study aimed to investigate the feasibility of PLLA/PCL (poly-L-lactic acid/polycaprolactone) by a comparison study of PLLA/PCL and PLLA scaffolds applied in bone regeneration. Thirty healthy mature New Zealand rabbits on which 15 mm distal ulna defect model had been established were selected and then were divided into three groups randomly: group A (repaired with PLLA scaffold), group B (repaired with PLLA/PCL scaffold), and group C (no scaffold) to evaluate the bone-remodeling ability of the implants. Micro-CT examination revealed the prime bone regeneration ability of group B in three groups. Bone mineral density of surgical site in group B was higher than group A but lower than group C. Meanwhile, the bone regeneration in both groups A and B proceeded with signs of inflammation for the initial fast degradation of scaffolds. As a whole, PLLA/PCL scaffoldsin vivoinitially degrade fast and were better suited to repair bone defect than PLLA in New Zealand rabbits. Furthermore, for the low mineral density of new bone and rapid degradation of the scaffolds, more researches were necessary to optimize the composite for bone regeneration.

Microleakage and SEM Analysis of Flowable Resin Used as a Sealant Following Three Fissure Preparation Techniques – An in vitro Study

2011 ◽  
Vol 35 (3) ◽  
pp. 277-282 ◽  
Author(s):  
TR Chaitra ◽  
VV Subba Reddy ◽  
GM Devarasa ◽  
TL Ravishankar
Keyword(s):  
Statistical Significance ◽  
In Vitro Study ◽  
Sem Analysis ◽  
Vitro Study ◽  
Blue Dye ◽  
Sem Images ◽  
Group A ◽  
Excellent Method ◽  
Group B

Objective: Preventive procedures using pit and fissure sealants are one of the important aspects of pediatric dental practice. The objectives of this in vitro study were to comparatively evaluate microleakage and resin tag penetration of a flowable resin used as a sealant on molars after preparation with Conventional, Enameloplasty and Fissurotomy techniques. Method: A total of 48 teeth were divided into 3 groups (n-16). Group A(CST),Group B (EST),and Group C (FT). Following the fissure preparation sealant was applied as per manufacturer's instructions. 8 samples in each group were used for Microleakage and Resin tag analysis. Microleakage analysis was done under Stereomicroscope after Methylene blue dye immersion by using scoring system. The resin tag analysis was done by measuring the length of resin tags on scanning electron microscope (SEM) images consisting of 10μm scale taken under optical zoom of 2000 X. Results: Significantly lesser microleakage score (P<0.01) and longer resin tag penetration (P<0.001) observed in EST(mean score 0.5±0.53) & (12.19±1.93μm) when compared to CST(mean score 1.75±0.89) & (5.96±1.84μm)and FT (mean score 1.5±0.53) & (6.76±1.82μm) which showed more microleakage and short resin tags. No statistical significance between CST and FT was found. Conclusion: EST has proven to be an excellent method for preparation of pits and fissures when Flowable composite is used as a sealant because of its lesser microleakage and longer resin tag penetration.

scholarly journals Properties of endotracheal tubes reprocessed by two procedures

2011 ◽  
Vol 51 (2) ◽  
pp. 73
Author(s):  
Elisa Elisa ◽  
S. H. Purwanto ◽  
A. T. Aman ◽  
Y. Pranoto ◽  
Kusmono Kusmono
Keyword(s):  
Photoelectron Spectroscopy ◽  
Testing Machine ◽  
Sem Analysis ◽  
Microstructure Analysis ◽  
Endotracheal Tubes ◽  
X Ray ◽  
Significant Difference ◽  
Large Particles ◽  
Group A ◽  
Group B

Background Reusing endotracheal tubes (EITs) has been performed in Indonesia with no evidence of its safety. Objective To evaluate sterility, as well as the mechanical, surface, and matrix properties of reused EITs following 2 different reprocessing procedures.Methods Reused EITs were cleaned and disinfected, then sterilized by ethylene oxide gas sterilization (group A) or dry heat sterilization (group B). New EITs were used as the standard for comparison. Microbes were identified and microbial counts were determined as colony forming units (CFUs). Evaluation of mechanical properties was perfonned by a Universal Testing machine. All samples underwent tensile and compression tests.Load defonnation curves were recorded from F max and strain at F max. Microstructure analysis was done using X􀁅ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX).Results Positive cultures of commensal bacteria were found in 2/12 samples in group A, and 5/17 samples in group B. T here was no statistically significant difference between them (P =0.07). Pseudomonas aeruginosa or other common pathogens were not found. Samples from both groups showed equal flaccidity, compared to the standard. Surface microstructure analysis of reused EITs 'With XPS and EDX showed degradation of the matrixcomponent. SEM analysis detected some large particles and fissures. EDX analysis on the large particles detected sodium and calcium signals. Altogether, signs of contamination and material damage were very strong.Conclusion Both reprocessing methods of reused EITs gave comparable results on sterility and mechanical behavior, but reprocessing may cause decreased surface and matrix quality. 

scholarly journals Bioactive Nanofiber-based Conduits in a Peripheral Nerve Gap Management- an Animal Model Study.

2020 ◽  
Author(s):  
Dębski Tomasz ◽  
Ewa Kijeńska-Gawrońska ◽  
Aleksandra Zołocińska ◽  
Katarzyna Siennicka ◽  
Wiktor Paskal ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Peripheral Nerve ◽  
Nerve Fiber ◽  
Muscle Mass ◽  
Peripheral Nerve Regeneration ◽  
Nerve Reconstruction ◽  
Fiber Density ◽  
Lewis Rats ◽  
Group A ◽  
Group B

Abstract Background: Peripheral nerve injuries (PNI) are trauma with severe squeals for patients’ quality of life. Currently, the gold standard of nerve reconstruction relies on primary coaptation. However, in case of a nerve gap, a reconstructive material for bridging is needed. Nerve conduits are an increasingly popular solution alternative to autografts or processed nerved allografts. The aim of the study was to examine the efficiency of a novel bioactive nanofiber-based tubular scaffold made of poly (L-lactic acid)-co-poly(ϵ-caprolactone), collagen, polyaniline and enriched with adipose-derived stem cells (ASCs) as a nerve conduit in a rat model.Methods: Poly (L-lactic acid)-co-poly(ϵ-caprolactone) scaffold was optimized and enriched with collagen (COL) and polyaniline (PANI) to create final (P(LLA-CL)-COL-PANI), tubular-shaped scaffold, manufactured with electrospinning technology. Parallelly, adipose tissue from 10 Lewis rats was harvested for ASC culture. 28 inbred male Lewis rats were divided into four even groups. Each animal underwent sciatic nerve transection and excision of a 10 mm nerve trunk fragment. In group A, nerve gap remained untouched, in B excised trunk was rotated and used as an autograft, in C nerve stumps were secured with P(LLA-CL)-COL-PANI conduit. In the D group gap was reconstructed with P(LLA-CL)-COL-PANI conduit enriched with ASCs. After 6-months of observation rats were sacrificed. Gastrocnemius muscles (operated and non-operated side) and reconstructed sciatic nerves were harvested for analyses. Muscles were weighted and underwent histological analysis. Nerves were processed and stained immunohistochemically with NF-200 to be analyzed with dedicated software for nerve fiber count. Results: No signs of rejection or excessive fibrosis was noted. Muscle mass ratio was highest in group B (0.77±0.05), then in C (0.74±0,04) and D (0.67± 0.07). Group A showed advanced atrophy of the muscle, each intervention prevented muscle mass decrease (p<0.0001), however, ASC addition decreased efficiency vs autograft (p<0.05). Nerve fiber count revealed a superior effect in the nerve fiber density observed in the group with the use of conduit vs autograft (D vs B p<0.0001, C vs B p<0.001). ASC added to the conduit decreased perineurium hyperplasia.Conclusion: P(LLA-CL)-COL-PANI conduits with ASC showed promising results in managing nerve gap by decreasing muscle atrophy and providing a favorable environment for peripheral nerve regeneration.

scholarly journals Efficacy of casein phosphopeptide varnish reducing dental hypersensitivity: A randomized clinical trial

2020 ◽  
Vol 3 (1) ◽  
pp. 7-11
Author(s):  
Rakhi A. Bapna ◽  
◽  
Neema Shetty ◽  
Aditi Mathur ◽  
Ashish Bali ◽  
...  
Keyword(s):  
Dentin Hypersensitivity ◽  
Test Results ◽  
Variable Intensity ◽  
Intergroup Comparison ◽  
Significant Difference ◽  
Group A ◽  
Chemical Stimuli ◽  
Casein Phosphopeptide ◽  
Group B ◽  
Dentinal Hypersensitivity

Introduction: Dentin hypersensitivity (DH) is the most common concern faced by most dentists in their day-to-day clinical practice. It is characterized by short-term, acute pain of variable intensity, which occurs in response to thermal, tactile, osmotic or chemical stimuli that cannot be attributed to any other type of defect or dental decay. Casein phosphopeptide varnish is a newer material used to treat dentinal hypersensitivity as it reduces demineralization and promotes remineralization. The aim of this study is to evaluate the efficacy of casein phosphopeptide based varnish (MI Varnish®) vs sodium fluoride-based varnish (Fluoritop SR® varnish) in treating dentinal hypersensitivity. Materials and methods: 40 subjects with a chief complaint of DH were divided into two equal groups, Group A (MI varnish®) and Group B (Fluoritop SR® varnish). Scaling and root planing was done and sensitivity was assessed using Visual analog scale (VAS) at baseline. On the 14th day, sensitivity was reassessed using VAS. The data obtained were subjected to statistical analysis using students‘t’ test. Results: On intragroup comparison, VAS scale showed a statistically significant difference for both the groups. On intergroup comparison, no statistical difference was found between both groups. (p = 0.139) Conclusions: From the above results, it can be concluded that MI varnish® and Fluoritop SR® varnish are equally efficacious in reducing dentinal hypersensitivity.

scholarly journals Comparison of Safety and Efficacy of Potassium Hydroxide versus Combination of Salicylic and Lactic Acid for Molluscum Contagiosum

2019 ◽  
Vol 8 (3) ◽  
pp. 131-134
Author(s):  
Durre-Shehwar Arshad Pirzada ◽  
Sumaira Abdullah ◽  
Qurat-ul Ain Zia ◽  
Syed Afaq Ahmed ◽  
Hamid Ali
Keyword(s):  
Salicylic Acid ◽  
Lactic Acid ◽  
Side Effects ◽  
Potassium Hydroxide ◽  
Pediatric Population ◽  
Molluscum Contagiosum ◽  
Final Visit ◽  
Local Irritation ◽  
Group A ◽  
Group B

Background: Molluscum contagiosum (MC) is a contagious cutaneous infection caused by MC virus, affecting mainly the pediatric population. It is characterized by flesh-colored umbilicated papules occurring on the skin surface. Potassium hydroxide (KOH) and salicylic acid plus lactic acid combination being keratolytic agents, are effective treatment options. The objective of the study was to compare the efficacy and safety of 10% KOH solution versus salicylic acid/ lactic acid combination in treating Molluscum contagiosum in pediatric population. Material and Methods: A total of 80 children (age range 1-13 years) with Molluscum contagiosum lesions were selected from the Dermatology Out-Patient Department, and were randomly allocated into two groups by lottery method. Group A was given topical 10% KOH solution and group B was given Salicylic plus Lactic acid preparations for topical application. Patients were followed after two weeks to see regression in size of the lesion, and development of any side-effects. Final visit was scheduled at 6 weeks. Statistical analysis was carried out using SPSS (version 16) with p value less than 0.05 considered as statistically significant. Results: Patients with MC (n = 80) were equally divided into group A (treated with 10% KOH) and group B (treated with combination of Salicylic acid and Lactic acid). After 6 weeks, complete remission from Molluscum contagiosum virus was seen in 29 (72.5%) patients of group A and 34 (85%) patients of group B. There were 12 (30%) patients in group A who developed local irritation and 4 (10%) developed local erythema after 6 weeks. None of the patients of group B had any such side effects. Conclusion: 10% KOH solution is as effective as the combination of Salicylic and Lactic acids for treatment of Molluscum contagiosum in pediatric population, however it is associated with side effects of local irritation and erythema.

scholarly journals Comparison between two methods for resin removing after bracket debonding

2012 ◽  
Vol 17 (6) ◽  
pp. 130-136 ◽  
Author(s):  
Rodrigo De Marchi ◽  
Luciana Manzotti De Marchi ◽  
Raquel Sano Suga Terada ◽  
Hélio Hissashi Terada
Keyword(s):  
Electron Microscopy ◽  
Sem Analysis ◽  
Single Step ◽  
Enamel Surface ◽  
The Other ◽  
Orthodontic Brackets ◽  
Control Group ◽  
Group A ◽  
Group B ◽  
Scanning Electron

OBJECTIVE: The aim of this study was to assess - using scanning electron microscopy (SEM) - the effectiveness of two abrasive discs, one made from silicon and one from aluminum oxide, in removing adhesive remnants (AR) after debonding orthodontic brackets. METHODS: Ten randomly selected bovine teeth were used, i.e., 2 in the control group, and the other 8 divided into two groups, which had orthodontic brackets bonded to their surface with Concise Orthodontic Adhesive (3M). The following methods were employed - in one single step - to remove AR after debracketing: Group A, Optimize discs (TDV) and Group B, Onegloss discs (Shofu), used at low speed. After removing the AR with the aforementioned methods, the teeth were prepared to undergo SEM analysis, and photographs were taken of the enamel surface with 50x magnification. Six examiners evaluated the photographs applying the Zachrisson and Årtun enamel surface index (ESI) system (1979). RESULTS: Group A exhibited minor scratches on the enamel surface as well as some AR in some of the photographs, while Group B showed a smoother surface, little or no AR and some abrasion marks in the photographs. No statistically significant differences were found between the two methods and the control group. CONCLUSIONS: The two abrasive discs were effective in removing the AR after bracket debonding in one single step.

EVALUATION OF THE DENTIN DESENSITIZING EFFICACY OF PASTE CONTAINING 8% ARGININE AND CALCIUM CARBONATE DURING DENTAL SCALING PROCEDURE

2016 ◽  
pp. 159-164
Author(s):  
Thi Bao Trinh Bui ◽  
Nu Nhu Y Pham
Keyword(s):  
Calcium Carbonate ◽  
Dentin Hypersensitivity ◽  
Base Line ◽  
Single Application ◽  
Dental Scaling ◽  
Scaling Procedure ◽  
Group A ◽  
Dental Diseases ◽  
Group B

Background: Dentin hypersensitivity (DH) is a short, sharp pain of teeth that can affect quality of life and treatment of oral dental diseases. The objective of this study was to evaluate the dentin desensitizing efficacy after single application of paste containing 8% Arginine and Calcium carbonate (A-C) during dental scaling procedure (DSP). Methods: This single-blind randomized clinical trial was conducted with 64 patients including 328 teeth with DH in which, group A: application of 8% A-C paste immediately before DSP and group B: application of 8% A-C paste immediately after DSP. On each patient, teeth with DH in both sides of jaw were randomized in 2 groups: the treatment was applied by 8% A-C paste, and the control was applied by pumice prophylaxis paste (PPP). DH scores were evaluated5 times: T0 (base-line), T1 (the group A: immediately after application of 8% A-C paste in the treatment and PPP in the control; the group B: similar to the group A but immediately after DSP), T2 (the group A: immediately after DSP; the group B: immediately after application of 8% A-C paste in the treatment and PPP in the control), T3 (1 week later), and T4 (4 weeks later). Results: In group A: the treatment had a reduced DH score immediately after applying the 8% A-C paste from T1 to T4 (the reduced percentage of DH of T1, T2, T3, and T4 were 43.11%, 43.11%, 44.89%, and 44.45% respectively) in comparison with baseline score. The group B: immediately after DSP, the DH score increased in both groups but the treatment had a reduced DH score immediately after applying the 8% A-C paste from T2 to T4 (42.32%, 44.81% and 43.98% respectively) in comparison with the T1. Conclusion: A single application of the 8% A-C paste immediately before or after DSP had an effect on reducing the DH immediately and this effect lasted 4 weeks later. Key words: Dentin hypersensitivity, paste containing 8% Arginine and Calcium carbonate

scholarly journals Effects of CRRT on renal function and toxin clearance in patients with sepsis: a case–control study

2021 ◽  
Vol 49 (9) ◽  
pp. 030006052110429
Author(s):  
Jinzhong Wang ◽  
Zhongyong Wu ◽  
Quan Wen ◽  
Xiaozhi Wang
Keyword(s):  
Renal Function ◽  
Lactic Acid ◽  
Treatment Group ◽  
Mortality Rate ◽  
Serum Creatinine ◽  
The Body ◽  
Urea Nitrogen ◽  
Routine Treatment ◽  
Group A ◽  
Group B

Objective To explore the effects of continuous renal replacement therapy (CRRT) on renal function and toxin clearance in patients with sepsis and concurrent acute kidney injury (AKI). Method A retrospective analysis was performed using the medical records of 115 patients with sepsis and AKI. Among them, 60 patients received routine treatment (group A) and 55 patients received CRRT plus routine treatment (group B). Result After treatment, the clearance rates of serum creatinine, lactic acid, and urea nitrogen were significantly lower in group A than in group B. The decrease in high-sensitivity C-reactive protein and tumor necrosis factor-α levels after treatment was significantly higher in group B than in group A. For the Acute Physiology Chronic Health Evaluation (APACHE) II and Sequential Organ Failure Assessment (SOFA) scores from the two groups, the scores were significantly lower in group B than in group A. The mortality rate within 28 days was significantly higher in group A than in group B. Conclusion CRRT can effectively improve the condition of patients with sepsis and AKI, promote elimination of toxins (serum creatinine, lactic acid, and urea nitrogen) from the body, and reduce the mortality rate.

Comparative SEM Study on the Effect of Irrigating the Radicular Dentine with NaOCl and EDTA Through Conventional Techniques and Diode Laser

2017 ◽  
Vol 68 (5) ◽  
pp. 916-920
Author(s):  
Alexandra Onac ◽  
Anamaria Florescu ◽  
Andreea Dana Tudose ◽  
Stefan Manea ◽  
Anna Maria Pangica ◽  
...  
Keyword(s):  
Diode Laser ◽  
Laser Diode ◽  
Water Solution ◽  
Conventional Technique ◽  
Sem Analysis ◽  
Smear Layer ◽  
Single Root ◽  
Sem Study ◽  
Group A ◽  
Group B

Since there are not many references in literature about the action of diode laser 940nm on the dentin and root smear layer, the aim of this study is to evaluate and compare, through SEM analysis, the efficiency of the Sodium hypochlorite 2% (CERKAMED) - EDTA 17% (CERKAMED) combination, operated in the middle and apical third of the root, by conventional syringe needle EndoEze and diode laser 940nm treatment. The study was performed on 40 single-root teeth, which were divided into two groups: group A consisting of 20 teeth, for which irrigation was performed only with the syringe and a top open side needle EndoEze Irrigator, resulting in a turbulence moving upward which efficiently irrigates the root canal and prevents periapical water solution; and group B consisting of 20 teeth, which benefited from irrigation associated with diode laser 940nm treatment. In the middle third and apical third smear layer removal was more effectively achieved when teeth benefited from irrigation associated with the laser diode treatment. In Endodontics, Diode laser is a good decontamination system, with biostimulation effect, which is important in healing the periapicale area. Significantly better results were obtained using 940nm laser diode associated with successive irrigation with 2% NaOCl and 17% EDTA, as compared to the conventional technique performed by syringe and lavage needle EndoEze Irrigator (Ultradent).

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