scholarly journals Physico-chemical Parameters and Antioxidant Activity of Romanian Sea Buckthorn Berries

2020 ◽  
Vol 70 (12) ◽  
pp. 4187-4192
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Content ◽  
Titratable Acidity ◽  
Sea Buckthorn ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Chemical Parameters ◽  
Physico Chemical ◽  
Total Flavonoids Content

Physico-chemical parameters of Romanian sea buckthorn fresh or dried berries, including titratable acidity (2.28±0.06 g malic acid/100 g FM), pH (2.60±0.08), contents of moisture (75.94±2.14 g/100 g FM), ash (2.83±0.11 g/100 g DM), ascorbic acid (1337.6±37.2 mg/100 g FM), oil (3.82±0.16 g/100 g FM), and macro elements, mainly K (11654 mg/kg DM), Mg (668 mg/kg DM), Ca (645 mg/kg DM), Na (401 mg/kg DM), and P (271 mg/kg DM), were evaluated in this paper. The most abundant volatile compounds found in the volatile fraction of berries were isopentyl isovalerate (30.8%), heptanal (11.7%), ethyl hexanoate (10.5%), ethyl isovalerate (8.1%), hexanal (7.5%), and ethyl 2-methylbutyrate (6.3%). Total phenolic content, total flavonoids content, and antioxidant activity (expressed as DPPH free radical scavenging activity by inhibition of DPPH) of extract solutions obtained from dried berries by MAE using ethanol/water mixture as a solvent were estimated at different ratios of solvent volume/vegetal material mass (15-30 mL/g) and ethanol/water (50/50, 60/40, 70/30, and 80/20 v/v). Inhibition of DPPH (22.61-48.60%) was well correlated with total phenolic content (4.42-10.04 mg caffeic acid equivalents/g DM) and total flavonoids content (5.37-11.14 mg quercetin equivalents/g DM). Keywords: Sea buckthorn, ascorbic acid, titratable acidity, oil, volatiles, minerals, total phenols, total flavonoids, antioxidant activity

scholarly journals Physico-Chemical Indices, Iso-alpha-Acid, Phenolic Contents and Antioxidant Activity of Commercial Beers

2013 ◽  
Vol 2 (4) ◽  
pp. 107 ◽  
Author(s):  
Antonietta Baiano ◽  
Carmela Terracone
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Principal Component ◽  
Titratable Acidity ◽  
Great Variability ◽  
Total Phenolic ◽  
Gluten Free ◽  
Phenolic Contents ◽  
Physico Chemical ◽  
Phenolic Concentration

<p>The effects of brewing style on physicochemical indices, iso-a-acids and antioxidant contents of commercial beers were investigated. A great variability was highlighted for all the considered parameters (sugar and alcohol contents, density, pH, titratable acidity, iso-a-acids, phenolic content, antioxidant activity). The beers having the highest iso-a-acids contents were Guinness Special Export Stout (which also showed the highest phenolic content), Chimay Pères Trappistes Triple, and Greene King IPA whereas the lowest values were detected in Cuveè De Ranke, Heineken Premium Quality Lager, and Riedenburger Brauhaus gluten free. The latter also had the lowest phenolic concentration. The antioxidant activity values strictly correlated with the total phenolic content but not with the iso-?-acid amount. The Principal Component Analysis failed in distinguishing beers based on their declared type/styles. This was a predictable result since the beer styles don’t represent a compositional classification but they are rather used to indicate commercial types.</p>

scholarly journals Variation of Bioactive Compounds and Antioxidant Activity of Jujube (Ziziphus jujuba) Fruits at Different Stages of Ripening

2018 ◽  
Vol 46 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Sina COSMULESCU ◽  
Ion TRANDAFIR ◽  
Violeta NOUR ◽  
Gheorghe ACHIM ◽  
Mihai BOTU ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Organic Acids ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Ripening Stage ◽  
Total Flavonoids Content ◽  
Ziziphus Jujuba

Fruits of two jujube (Ziziphus jujuba Mill.) cultivars were collected at four stages of ripening and analyzed in terms of chemical compounds. The cultivars were grown under the climatic conditions of the Sub-Carpathian area Ramnicu-Valcea in Romania (45°6′17″N, 24°22′32″E). Total phenols content, total flavonoids content, organic acids from jujube fruits and related antioxidant activities were investigated during the ripening stages. The antioxidant activity of alcoholic extracts was investigated by the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. Total phenolic content was determined by using the Folin-Ciocalteu assay, while determination of flavonoids was done by aluminium nitrate colorimetric method, and organic acids were separated by reversed phase chromatography. The results obtained have indicated significant differences (p < 0.05) found in bioactive compounds and antioxidant activity, depending on cultivars and ripening stage. Total phenolic content ranged from 1634.44 to 472.38 mg GAE/100 g in methanolic extracts of the two varieties studied, while total flavonoids content ranged from 19.9 to 48.5 mg QE/100 g. As regards the influence of ripening stage, the results show that the highest antioxidant activity was recorded in the stage of white maturity. The impact of maturity stage was significant on organic acid distribution of jujube extracts, and ascorbic acid is the major organic acid found in jujube fruits, followed by malic acid and lactic acid. Jujube fruits are rich in bioactive compounds and can be a complement in human’s healthy eating.

scholarly journals Glucosinolate profiles by HPLC-DAD, phenolic compositions and antioxidant activity of Eruca vesicaria longirostris: Impact of plant part and origin

2016 ◽  
Vol 5 (5) ◽  
pp. 528-539
Author(s):  
Saoussen Bouacida ◽  
Hayet Ben Haj koubaier ◽  
Ahmed Snoussi ◽  
Marie Laure Fauconnier ◽  
Nabiha Bouzouita
Keyword(s):  
Antioxidant Activity ◽  
Statistical Analysis ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Plant Part ◽  
The Other ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content ◽  
Seed Extracts

The glucosinolate profiles, phenol and flavonoid contents and the antioxidant activity of Eruca vesicaria longirostris were studied for different organs and origins. Eleven desulpho-glucosinolates (DS-GLSs) were isolated and quantified by lipid chromatography- DAD. Similarity between profiles was obtained. Total DS-GLS content, expressed as sinigrin equivalents (SE) revealed a certain variabilily ranging between (76.07-45.61), (27.01-13.53), (4.52 -18.01), (9.39-3.37) and (1.16-13.99) µmol /g DW for seeds, flowers, leaves, roots and stems, respectively. Results showed that seeds are rich in phenolics as they contain highest amounts of phenolics ranging from 27.6±0.5 to 33.47±0.5 mg GAE/g extract as compared to all other parts. Leaves and flowers had a significantly higher total phenolic content than stems and roots in all samples (p < 0.05). According to statistical analysis, the investigated seed extracts with values between (16.20±0.10-18.50±0.10 mg QE/g) exhibited the highest total flavonoids content, followed by leaves (13.00±0.40-15.80±0.30mg QE/g), flowers (10.40±0.40-12.90±0.90 mg QE/g) and stems (7.80±0.20- 9.80±0.70 mg QE/g). Antioxidant activity tested by DPPH, ABTS and FRAP assays, was higher for seeds, leaves and flowers than the other studied organs. These organs were characterized by a significantly high content in glucoerucin, nasturtin and epiprogroitrin, respectively.

scholarly journals Bioactive Compounds, Antioxidant Activity and Nutritional Quality of Different Culinary Aromatic Herbs

2017 ◽  
Vol 45 (1) ◽  
pp. 179-184 ◽  
Author(s):  
Violeta NOUR ◽  
Ion TRANDAFIR ◽  
Sina COSMULESCU
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Acid Content ◽  
Nutritional Quality ◽  
Radical Scavenging ◽  
Ascorbic Acid Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content ◽  
Aromatic Herbs

In the present work, four of the most widely used culinary herbs (parsley, dill, lovage and celery leaves) in the Romanian traditional cuisine were assessed for some nutritional quality parameters (moisture content, titratable acidity, ascorbic acid content and mineral content), total phenolics content, total flavonoids content and antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. In addition, high-performance liquid chromatography (HPLC) coupled with diode-array detection was applied for the identification and quantification of some individual phenolic compounds in the selected aromatic herbs. Although parsley showed the highest ascorbic acid content (206.32 mg/100 g fw), it registered the lowest antioxidant activity. Fresh lovage recorded the highest total phenolic content (577.04 mg GAE/100 g), total flavonoids content (298.38 mg QE/100 g) and antioxidant activity, followed by dill and celery leaves. Among the identified phenolic acids, ellagic acid was predominant in lovage and celery leaves whereas sinapic and vanillic acids were prevalent in dill. Among flavonoids, myricetin was predominant in parsley and celery leaves whereas rutin was predominant in dill and lovage.

scholarly journals Antioxidant activity and phenolic acid content of selected vegetable broths

2017 ◽  
Vol 35 (No. 6) ◽  
pp. 469-475 ◽  
Author(s):  
Filiz Bilge Ertekin ◽  
Korkmaz Nazli ◽  
Budak Nilgun H ◽  
Seydim Atif C ◽  
Seydim Zeynep B Guzel
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Acid ◽  
Acid Content ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ascorbic Acid Content ◽  
Total Phenolic ◽  
Green Beans ◽  
Fresh Vegetables

The antioxidant activity and content of phenolic substances in vegetable broths were determined. Green beans, beetroots, courgettes, onions, parsley, carrots, cabbages, celery, broccoli, spinach, cauliflowers, and tomatoes were subjected to boiling. Fresh vegetables and vegetable broths were analysed for ascorbic acid content, total phenolic content, ORAC and TEAC values. Phenolic acids were quantified using HPLC. The ascorbic acid content of vegetables ranged from 5–109 mg/100 ml, while no ascorbic acids could be detected in vegetable broths. Total phenolic content was between 17–1729 mg GAE/l for all samples. ORAC and TEAC values of vegetable broths were between 0–3 µmol TE/ml and 0–2 µmol TE/ml, respectively. Gallic, chlorogenic, caffeic, p-coumaric, and ferulic acid were detected in both fresh vegetables and vegetable broths. The highest phenolic acid content was observed in water in which beetroots were boiled. It was found that the vegetable broths of beetroots, celery stalks, cabbages, parsley and broccoli harboured remarkable antioxidant activity.

Antioxidant and antimicrobial capacities of ethanolic extract of Pergularia daemia leaves: a possible substitute in diabetic management

2016 ◽  
Vol 13 (3) ◽  
Author(s):  
Joseph Adusei Sarkodie ◽  
Sylvia Afriyie Squire ◽  
Emelia Oppong Bekoe ◽  
Charles Yaw Fosu Domozoro ◽  
Irene Awo Kretchy ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Colorimetric Assay ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content

Abstract: The leaves of: The total phenolic content, total flavonoids content, radical scavenging activity and reducing power assays were estimated using Folin–Ciocalteu method, aluminium chloride colorimetric assay, Fe: The results showed that: These findings justify the folkloric use of

scholarly journals TOTAL PHENOLIC AND FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITY OF VITEX MONTEVIDENSIS (SPRENG) MOLDENKE - VERBENACEAE

2016 ◽  
Vol 38 (3) ◽  
pp. 1197
Author(s):  
Sideney Becker Onofre ◽  
Dirceu Abatti ◽  
Amarildo Antonio Tessaro ◽  
Alessandra Buss Tessaro
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Phenols ◽  
Degenerative Diseases ◽  
Flavonoid Content ◽  
The Mean ◽  
Phenolic And Flavonoid

In recent years, a substantial amount of evidence has pointed to the key role of free radicals and other oxidants as the main culprits for aging and degenerative diseases associated with aging, such as cancer, cardiovascular diseases, cataract, decline of the immune system and brain dysfunctions. The objective of this work was therefore to detect variations in total phenol and flavonoid content, and in antioxidant activity of samples of V. montevidensis. Dried samples were submitted to extraction to obtain the hexane, ethyl acetate and ethanol fractions. Phytochemical prospecting and an analysis of the phenolic content and antioxidant activity was carried out. The data was analyzed according to the mean±standard deviation and submitted to analysis of variance followed by Tukey's test with a significance of (p < 0.05). Flavonoids, tannins, coumarins, terpenoids, steroids, alkaloids and anthraquinones were detected in the samples. The content of total flavonoids varied between 3.18±0,58 and 7.22±0,17 g/100g, while the total phenols ranged from 1.13±0.16 to 18.44±0.21 g/100g. The extracts produced EC50 between 339.75±6.56 and 278.33±23.11 μg/mL.

scholarly journals Bioactive Content of Rose Hips of Different Wildly Grown Rosa dumalis Genotypes

2016 ◽  
Vol 44 (2) ◽  
pp. 472-476 ◽  
Author(s):  
Sevket ALP ◽  
Sezai ERCISLI ◽  
Tunde JURIKOVA ◽  
Ozlem CAKIR ◽  
Sadiye GOZLEKCI
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Fresh Weight ◽  
Total Carotenoids ◽  
Treatment And Prevention ◽  
Bioactive Content ◽  
Total Flavonoids Content ◽  
Genotype E

A rose hip is the fruit of a rose plant and mostly belongs to Rosa canina and Rosa dumalis. These species are genuine found as wild in natural conditions, while in some countries are cultivated for their hips that are picked and used in a wide variety of preparations. Because they contain a variety of antioxidants, carotenoids, flavonoids, polyphenols, leucoanthocyanins and catechins, rose hips are considered to be a good cancer preventative. The Rosa genus has been the focus of many recent studies due to its potential benefic effects on treatment and prevention of several diseases. However, there are few in vitro studies concerning its composition and antioxidant capacity. The aim of the present study was to determine bioactive content, including ascorbic acid, total phenolics, total flavonoids, total carotenoids and antioxidant activity of Rosa dumalis genotypes growing wild in Erzurum province located eastern Anatolia. The results revealed that fruits of Rosa dumalis different genotypes were rich rich in terms of vitamin C, which ranged from 402 to 511 mg/100 g fresh weight base. The total phenolic content varied from 297 to 403 mg/100 g fresh weight. The genotype 'E-09' had the highest total flavonoids content (229 mg quercetin equivalent/100 g fresh weight) and the lowest value was attributed to genotype 'E-04' with 143 mg/ quercetin equivalent/100 g fresh weight. The antioxidant activity of the genotypes was between 12.9-28.6 μg Trolox/ml samples. The results revealed that there was enough diversity among Rosa dumalis genotypes for bioactive content and promising genotypes, with high bioactive content, were determined, which can become study material for future breeding activities.

Phytochemical and antioxidant activity of Cadaba farinosa Forssk stem bark extracts

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Emmanuel M. Halilu ◽  
Abdullahi M. Abdurrahman ◽  
Sylvester N. Mathias ◽  
Chinenye J. Ugwah-Oguejiofor ◽  
Muntaka Abdulrahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Free Radical Scavenging ◽  
Total Phenolic

Abstract Cadaba farinosa is used in traditional medicine for treatment of cancer, diabetes, and rheumatism. The research was aimed at evaluating the phytochemical and antioxidant activity of the extracts. The powdered stem bark was extracted successively with the aid of Soxhlet extractor using n-hexane, ethyl acetate, and methanol. The resulting extracts were concentrated on rotary evaporator and the percentage yields were calculated. The phytochemical and TLC profiles of the extracts were studied. The antioxidant activity of the extracts and ascorbic acid (standard) were determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay. The total phenolic content of the extracts and tannic acid (standard) were evaluated using Folin–Ciocalteu reagent. The percentage yields of n-hexane, ethyl acetate, and methanol extracts were found to be 1.19, 1.37, and 13.93%, respectively. The phytochemical screening revealed the presence of tannins, flavonoids, saponins, cardiac glycosides, alkaloids, and triterpenoids. The TLC profiles of the extracts revealed the presence of compounds as evidenced from their R f values. The total phenolic content of ethyl acetate and methanol extracts were found to be 135 and 112 mg, respectively. The free radical scavenging activity demonstrated by the extracts was comparable to ascorbic acid. The ethyl acetate extract had higher phenolic content and demonstrated the highest free radical scavenging with IC50 31.07 mg/mL. The results of research have provided strong preliminary evidence of antioxidant activity which may be because of phenolic compounds in the extracts.

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