scholarly journals Glucosinolate profiles by HPLC-DAD, phenolic compositions and antioxidant activity of Eruca vesicaria longirostris: Impact of plant part and origin

2016 ◽  
Vol 5 (5) ◽  
pp. 528-539
Author(s):  
Saoussen Bouacida ◽  
Hayet Ben Haj koubaier ◽  
Ahmed Snoussi ◽  
Marie Laure Fauconnier ◽  
Nabiha Bouzouita
Keyword(s):  
Antioxidant Activity ◽  
Statistical Analysis ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Plant Part ◽  
The Other ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Total Flavonoids Content ◽  
Seed Extracts

The glucosinolate profiles, phenol and flavonoid contents and the antioxidant activity of Eruca vesicaria longirostris were studied for different organs and origins. Eleven desulpho-glucosinolates (DS-GLSs) were isolated and quantified by lipid chromatography- DAD. Similarity between profiles was obtained. Total DS-GLS content, expressed as sinigrin equivalents (SE) revealed a certain variabilily ranging between (76.07-45.61), (27.01-13.53), (4.52 -18.01), (9.39-3.37) and (1.16-13.99) µmol /g DW for seeds, flowers, leaves, roots and stems, respectively. Results showed that seeds are rich in phenolics as they contain highest amounts of phenolics ranging from 27.6±0.5 to 33.47±0.5 mg GAE/g extract as compared to all other parts. Leaves and flowers had a significantly higher total phenolic content than stems and roots in all samples (p < 0.05). According to statistical analysis, the investigated seed extracts with values between (16.20±0.10-18.50±0.10 mg QE/g) exhibited the highest total flavonoids content, followed by leaves (13.00±0.40-15.80±0.30mg QE/g), flowers (10.40±0.40-12.90±0.90 mg QE/g) and stems (7.80±0.20- 9.80±0.70 mg QE/g). Antioxidant activity tested by DPPH, ABTS and FRAP assays, was higher for seeds, leaves and flowers than the other studied organs. These organs were characterized by a significantly high content in glucoerucin, nasturtin and epiprogroitrin, respectively.

scholarly journals Variation of Bioactive Compounds and Antioxidant Activity of Jujube (Ziziphus jujuba) Fruits at Different Stages of Ripening

2018 ◽  
Vol 46 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Sina COSMULESCU ◽  
Ion TRANDAFIR ◽  
Violeta NOUR ◽  
Gheorghe ACHIM ◽  
Mihai BOTU ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Organic Acids ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Ripening Stage ◽  
Total Flavonoids Content ◽  
Ziziphus Jujuba

Fruits of two jujube (Ziziphus jujuba Mill.) cultivars were collected at four stages of ripening and analyzed in terms of chemical compounds. The cultivars were grown under the climatic conditions of the Sub-Carpathian area Ramnicu-Valcea in Romania (45°6′17″N, 24°22′32″E). Total phenols content, total flavonoids content, organic acids from jujube fruits and related antioxidant activities were investigated during the ripening stages. The antioxidant activity of alcoholic extracts was investigated by the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. Total phenolic content was determined by using the Folin-Ciocalteu assay, while determination of flavonoids was done by aluminium nitrate colorimetric method, and organic acids were separated by reversed phase chromatography. The results obtained have indicated significant differences (p < 0.05) found in bioactive compounds and antioxidant activity, depending on cultivars and ripening stage. Total phenolic content ranged from 1634.44 to 472.38 mg GAE/100 g in methanolic extracts of the two varieties studied, while total flavonoids content ranged from 19.9 to 48.5 mg QE/100 g. As regards the influence of ripening stage, the results show that the highest antioxidant activity was recorded in the stage of white maturity. The impact of maturity stage was significant on organic acid distribution of jujube extracts, and ascorbic acid is the major organic acid found in jujube fruits, followed by malic acid and lactic acid. Jujube fruits are rich in bioactive compounds and can be a complement in human’s healthy eating.

Phenolic Compounds and Antioxidant Activity of Olea europaea L. Fruits and Leaves

2006 ◽  
Vol 12 (5) ◽  
pp. 385-395 ◽  
Author(s):  
S. Silva ◽  
L. Gomes ◽  
F. Leitão ◽  
A. V. Coelho ◽  
L. Vilas Boas
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Olive Tree ◽  
Total Phenolic ◽  
Total Antioxidant Activity ◽  
Total Antioxidant ◽  
Olive Seeds ◽  
Seed Extracts

Extracts of leaves, fruits and seeds of olive tree cultivars of Trás-os-Montes e Alto Douro (Portugal) were analysed by reverse phase HPLC with diode array detection and mass spectrometry (MS). This methodology allowed the identification of some common phenolic compounds, namely, verbascoside, rutin, luteolin-7-glucoside, oleuropein and hydroxytyrosol. Moreover the hyphenation of HPLC with MS enabled the identification of nüzhenide in olive seeds. An oleuropein glucoside was also detected in olive tree leaves. The total phenolic content was determined with the Folin Denis reagent and the total antioxidant activity with the ABTS method. There is a correlation between total antioxidant activity and total phenolic content with the exception of the seed extracts analysed. The apparent high antioxidant activity of seed extracts may be due to nüzhenide, a secoiridoid that is the major phenolic component of olive seeds. These results suggest a possible application of olive seeds as sources of natural antioxidants.

scholarly journals Phytochemical Analysis and Antimicrobial Activity of Iris kashmiriana and Iris ensata Extracts against Selected Microorganisms

2018 ◽  
Vol 8 (6) ◽  
pp. 28-34
Author(s):  
Javeed Iqbal Wagay ◽  
Kirti Jain
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Iris Ensata ◽  
Total Flavonoids Content ◽  
Phytochemical Constituent

Infectious diseases are the second leading cause for worldwide death. Treatment of infections continues to be difficult in modern time because of the severe side effects of some drugs and the growing resistance to antibacterial agents. Over the past few decades the use of antibiotics is under threat as many commonly used antibiotics have become less effective against certain illnesses due to emergence of multi drug-resistant bacteria. In the present study the effects of 3 types of solvents, chloroform, ethyl acetate and methanol were investigated to determine the presence of various phytochemical constituent, total phenolic content, total flavonoids content and in vitro antimicrobial activity from rhizomes of Iris kashmiriana (Kashmir Iris) and Iris ensata (Japanese Iris), belong to family Iridaceae. The reason for selecting in vitro method was to minimize the usage of experimental animals. The antimicrobial activity of chloroform, ethyl acetate and methanol extract of rhizomes of Iris kashmiriana and Iris ensata were evaluated on bacterial strains of Bacillus cereus, Pseudomonas auregenosa, Proteus vulgaris and Eschirichia coli and fungal strains of Candida albicans and Aspergillus niger by agar well diffusion method. The preliminary phytochemical studies and quantitative analysis of alkaloids, phenol and flavonoids were performed by well reported method. These extracts were further subjected to TLC (Thin layer chromatography analysis).  The chemical contents of the Iris kashmiriana and Iris ensata were presented as total phenolic content and total flavonoids content. Phytochemical screening of the extract showed the presence of some common compounds like phenols, terpenoids, flavonoids, carbohydrate etc. The antimicrobial potential of the plant extract was evaluated against different bacterial species which shows significant inhibitory action against all the tested bacterial and fungal strain. Methanolic extract was found to be more active than chloroform and ethyl acetate extracts. It reveals that the methanol soluble components of the plant are highly active against the above mentioned microorganism. Keywords: Iris kashmiriana, Iris ensata, Phytochemical constituent, Total phenolic content, Total flavonoids content, In vitro antimicrobial activity

Total Phenolic and Flavonoid Content and Antibacterial Properties of Polygonatum orientale Desf and Tilia dasystyla

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Parichehr Hanachi ◽  
Roshanak Zarringhalami ◽  
Ertugrul Kaya
Keyword(s):  
Staphylococcus Aureus ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Total Flavonoids ◽  
Flavonoid Content ◽  
Water Solvent ◽  
Total Flavonoids Content ◽  
Phenolic And Flavonoid

Background: Secondary metabolites of plants such as phenol and flavonoids can neutralize free radicals. The antioxidant and cytotoxic activities of plants are related to phenolic or flavonoids compounds. The occurrence of drug resistance to antimicrobial drugs has led to the use of medicinal herbs in the treatment of infections. Antibiotic resistant of Staphylococcus aureus has become a major problem in the treatment of diseases. Objectives: The present study aimed to determine total phenolic content (TPC) and total flavonoids content (TFC) of Polygonatum orientale Desf and Tilia dasystyla and evaluate their antibacterial effects on Staphylococcus aureus bacterium. Total phenolic content of Polygonatum orientale Desf and Tilia dasystyla has not been previously investigated. Methods: Total phenolic and flavonoid content of P. orientale Desf and T. dasystyla extracts were determined using colorimetric methods of Folin-Ciocalteu and aluminum chloride. Antimicrobial activities of the extracts were evaluated by microdilution broth and disc diffusion methods to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. Results: The results showed that total flavonoids content of Polygonatum orientale Desf with the value of 7.9 ± 0.040 mg/g DW extracted with diluted water solvent and boiling method and total phenolic contents of Tilia dasystyla with the value of 62.13 ± 0.073 mg/g DW extracted with methanol solvent and boiling method were the highest amount. Methanol extract of Polygonatum orientale Desf had more antibacterial activities with the MBC and MIC values of 0.140 mg/ mL and 8 ± 0.4 mm zone of inhibition. Conclusions: T. dasystyla and P. orientale Desf contain bioactive compounds such as phenolic and flavonoids that can be used as a promising option in pharmacognostical studies for the treatment of S. aureus infections.

scholarly journals Physico-chemical Parameters and Antioxidant Activity of Romanian Sea Buckthorn Berries

2020 ◽  
Vol 70 (12) ◽  
pp. 4187-4192
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Content ◽  
Titratable Acidity ◽  
Sea Buckthorn ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Chemical Parameters ◽  
Physico Chemical ◽  
Total Flavonoids Content

Physico-chemical parameters of Romanian sea buckthorn fresh or dried berries, including titratable acidity (2.28±0.06 g malic acid/100 g FM), pH (2.60±0.08), contents of moisture (75.94±2.14 g/100 g FM), ash (2.83±0.11 g/100 g DM), ascorbic acid (1337.6±37.2 mg/100 g FM), oil (3.82±0.16 g/100 g FM), and macro elements, mainly K (11654 mg/kg DM), Mg (668 mg/kg DM), Ca (645 mg/kg DM), Na (401 mg/kg DM), and P (271 mg/kg DM), were evaluated in this paper. The most abundant volatile compounds found in the volatile fraction of berries were isopentyl isovalerate (30.8%), heptanal (11.7%), ethyl hexanoate (10.5%), ethyl isovalerate (8.1%), hexanal (7.5%), and ethyl 2-methylbutyrate (6.3%). Total phenolic content, total flavonoids content, and antioxidant activity (expressed as DPPH free radical scavenging activity by inhibition of DPPH) of extract solutions obtained from dried berries by MAE using ethanol/water mixture as a solvent were estimated at different ratios of solvent volume/vegetal material mass (15-30 mL/g) and ethanol/water (50/50, 60/40, 70/30, and 80/20 v/v). Inhibition of DPPH (22.61-48.60%) was well correlated with total phenolic content (4.42-10.04 mg caffeic acid equivalents/g DM) and total flavonoids content (5.37-11.14 mg quercetin equivalents/g DM). Keywords: Sea buckthorn, ascorbic acid, titratable acidity, oil, volatiles, minerals, total phenols, total flavonoids, antioxidant activity

scholarly journals Phenolic Composition and Antioxidant Activity of Peel, Pulp and Seed Extracts of Different Clones of the Turkish Grape Cultivar ‘Karaerik’

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2154
Author(s):  
Muhammed Kupe ◽  
Neva Karatas ◽  
Mehmet Settar Unal ◽  
Sezai Ercisli ◽  
Mojmir Baron ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenol ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Grape Cultivar ◽  
Seed Extracts

The Erzincan plain is one of the richest regions in Turkey in terms of plant biodiversity. In this region, the famous grape cultivar ‘Karaerik’ has always dominated grape production due to its berry characteristics. The cultivar shows great morphological variation at clonal level. In this study, the total phenolic content and antioxidant activity of peel, pulp and seed extracts of nine ‘Karaerik’ clones sampled from same location were investigated. The Folin–Ciocalteu method was used to determine the total phenolic content of peel, pulp and seed extracts of nine clones. To determine antioxidant activity, three well known assays such as DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate), FRAP (Ferric Reducing Antioxidant Power) and TEAC (Trolox Equivalent Antioxidant Capacity) were used. In addition, the correlation between total phenol content and DPPH, FRAP and TEAC was determined. Results showed that among the tissues, seed samples in berries of all clones had the highest total phenol content and antioxidant activity determined by three assays. Seed samples were followed by peel and pulp for total phenolic content and antioxidant activity. Among the nine ‘Karaerik’ clones, Clone 8 had the highest total phenolic content (149 mg GAE/100 g FW) while Clone 3 had the lowest (111 mg GAE/100 g FW). Peel, pulp and seed samples of nine ‘Karaerik’ clones showed strong antioxidant activity in DPPH, FRAP and TEAC assays. In particular, grape seeds were found rich for better in phenolic compounds including gallic acid, quercetin, catechin, chlorogenic acid, caffeic acid and p-coumaric acid. Clones such as 7, 8 and 9 higher antioxidant activity may present great potential for grape breeders and the food industry as well as health-conscious consumers.

scholarly journals Influence of processing on the phenolic compounds of feijoa pulp (Feijoa sellowiana)

2020 ◽  
Vol 11 ◽  
Author(s):  
Ana Carolina Leme Castelucci ◽  
Nataly Maria Viva De Toledo ◽  
Fernanda Francetto Juliano ◽  
Paula Porrelli Moreira Da Silva ◽  
Marta Helena Fillet Spoto
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Principal Component ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Phenolic Composition ◽  
And Control

The effect of processing on potential changes of antioxidant activity (DPPH and ABTS), total phenolic content (TPC) and total flavonoids (FLAV) of feijoa pulp during storage was investigated. In addition, possible modifications in the phenolic composition of the pulp. The pulp was subjected to refrigeration (control, 5 °C), pasteurization (85 °C/5 min) and irradiation (2.0 kGy). TPC, FLAV, DPPH and ABTS were measured during 28 days, whereas the identification and quantification of phenolic compounds were conducted after processing. The results were submitted to principal component analysis (SAS 9.4). Pasteurization preserved TPC, DPPH and ABTS for 21 days, while in the irradiated samples and control, the values were decreased and FLAV were maintained at high levels. The profile of phenolic compounds was different for each sample with six compounds being identified.

scholarly journals RELATIONSHIP BETWEEN TOTAL PHENOLICS CONTENTS, TOTAL FLAVONOIDS CONTENTS AND ANTIOXIDANT ACTIVITY IN DIFFERENT VEGETABLES IN HUE

2018 ◽  
Vol 127 (1A) ◽  
Author(s):  
Khanh Thuy Linh Nguyen ◽  
Thi Hang Dao ◽  
Nhat Quynh Le ◽  
Thi Hien Thu Pham ◽  
Huu Tien Nguyen
Keyword(s):  
Antioxidant Activity ◽  
Biological Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Colorimetric Assay ◽  
Dry Weight ◽  
Total Phenolic ◽  
Total Flavonoids ◽  
Plant Phenolics

<p>The increasing interest in powerful biological activity of plant phenolics and flavonoids outlined the necessity of determining their contents in vegetables. The study comprised 10 vegetable species commonly consumed in Hue. The total phenolic content was determined by using the Folin-Ciocalteu assay. The content of total flavonoids was measured also spectrophotometrically by using the aluminum chloride colorimetric assay. Antioxidant activity of extracts were expressed as IC<sub>50</sub> values (µg/ml). Values in IC<sub>50</sub> ranged from 2.08 to 51.06. The total phenolic content ranged from 3.18 ± 0.03 to 11.87 ± 0.06 (mg/g) of dry weight of extract expressed as gallic acid equivalents. The total flavonoid concentrations varied from 2.33 ± 0.03 to 19.47 ± 0.21 (mg/g), expressed as quercetin equivalents.</p>

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