scholarly journals Chemical composition and larvicidal activity of the essential oil of Pimenta dioica leaves

2022 ◽  
Vol 21 (2) ◽  
pp. 207-214
Author(s):  
Paulo Roberto Barros Gomes ◽  
◽  
Silvio Carvalho Marinho ◽  
Gustavo Oliveira Everton ◽  
Eduardo Fonseca Silva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Larvicidal Activity ◽  
Confidence Level ◽  
Lethal Concentration ◽  
Major Constituent ◽  
Main Constituent ◽  
The Third ◽  
Third Stage ◽  
Pimenta Dioica ◽  
Muench Method

In this study, we investigated the main constituent, the predominant class and biological activity of the essential oil extracted from the leaves of Pimenta dioica and the pattern of the major constituent against larvae in the third stage of Aedes aegypti. For this reason, we extracted the oil by hydrodistillation, identified its components by gas chromatography coupled with mass spectrometry (GC/MS) and calculated the lethal concentration (LC50) of the larvicidal activity using the Reed-Muench method. The results show that the oil consists mainly of eugenol, in which the phenylpropanoid class predominated and the lethal concentration, LC50, was 38.86 μg mL-1at a confidence level of 2.25 μg mL-1, while the eugenol standard presented LC5079.75 μg mL-1at a confidence level of 2.10 μg mL-1. Given the facts, we conclude that the oil is more active than the standard and that it has the potential to replace chemical larvicides.

scholarly journals Larvicidal activity, molluscicide and toxicity of the essential oil of Citrus limon peels against, respectively, Aedes aegypti, Biomphalaria glabrata and Artemia salina

2019 ◽  
Vol 44 (4) ◽  
pp. 85 ◽  
Author(s):  
Paulo Roberto Barros Gomes ◽  
Marlucy Bezerra Oliveira ◽  
Dionney Andrade De Sousa ◽  
Jeremias Caetano Da Silva ◽  
Romer Pessôa Fernandes ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Biological Activities ◽  
Biomphalaria Glabrata ◽  
Artemia Salina ◽  
World Health ◽  
Lethal Concentration ◽  
Major Constituent ◽  
Citrus Limon

In this present work, we tested the larvicidal activity, molluscicide and toxicity of the oil extracted from Citrus limon peels, respectively against third stage larvae of Aedes aegypti, snail Biomphalaria glabrata, and Artemia salina. For this, we extract the essential oil by hydrodistillation. Then, we identified and quantified the components by gas chromatography coupled to mass spectrometry (GC-MS). We tested the larvicidal and molluscicidal activity, respectively, using the method adopted by the Brazilian Ministry of Health and the World Health Organization. We calculated the lethal concentration (LC50) from the Probit method for the three biological activities with 95 %. The results of the chromatographic analysis showed that the oil has 58.81% of Limonene (major constituent) and 0.11% α-Mulene (minority component). The essential oil presented lethal concentration (LC50) for larvicidal activity, molluscicide and toxicity, respectively at 15.48, 13.05 and 743.35 mg∙L-1. Therefore, the essential oil is active against larvae of A. aegypti and snail B. glabrata and non-toxic against larvae of A. salina.

scholarly journals Larvicidal Activity of Essential Oil of Syzygium aromaticum (Clove) in Com-parison with Its Major Constituent, Eugenol, against Anopheles stephensi

2019 ◽  
Author(s):  
Mahmoud Osanloo ◽  
Mohammad Mehdi Sedaghat ◽  
Fariba Esmaeili ◽  
Amir Amani
Keyword(s):  
Essential Oil ◽  
Larvicidal Activity ◽  
Lower Risk ◽  
High Performance ◽  
Anopheles Stephensi ◽  
Lower Cost ◽  
Major Constituent ◽  
Syzygium Aromaticum ◽  
Clove Essential Oil ◽  
Lc50 And Lc90

Background: In this study, larvicidal activity of clove essential oil (EO), as a green and relatively potent larvicide, was compared with its main constituent, Eugenol, against Anopheles stephensi. Methods: High-performance liquid chromatography (HPLC) was used to determine the amount of eugenol, major constituent of clove EO. In addition, larvicidal activity of clove EO and eugenol was evaluated against An. stephensi. Results: The amount of eugenol in clove EO was determined as 67% using HPLC analysis. LC50 and LC90 of clove EO (57.49 and 93.14ppm, respectively) were significantly lower than those of eugenol (86.96 and 128.18 ppm, re­spectively). Conclusion: EO showed more effective than its major component. Considering the lower cost of the essential oil and lower risk in occurrence of resistance in larvae, use of clove EO is preferred as larvicide in comparison with eugenol, against An. stephensi.

scholarly journals Chemical study and larvicidal activity against Aedes aegypti of essential oil of Piper aduncum L. (Piperaceae)

2013 ◽  
Vol 85 (4) ◽  
pp. 1227-1234 ◽  
Author(s):  
GISELE L. OLIVEIRA ◽  
SHEILA K. CARDOSO ◽  
CELIO R. LARA JUNIOR ◽  
THALLYTA M. VIEIRA ◽  
ELSIE F. GUIMARAES ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Mortality Rate ◽  
Essential Oil ◽  
Larvicidal Activity ◽  
Inflammatory Diseases ◽  
Folk Medicine ◽  
Chemical Study ◽  
Major Constituent ◽  
Flame Ionization ◽  
Piper Aduncum

ABSTRACT Piper aduncum L. is used in folk medicine to treat respiratory and inflammatory diseases. The aim of this study was to analyze the essential oil from leaves of P. aduncum collected in the Brazilian Cerrado, North of Minas Gerais, as well as to evaluate the larvicidal activity of this oil and of its major constituent. The essential oil was analyzed by gas chromatography coupled to flame ionization detector and gas chromatography coupled to mass spectrometry that allowed characterizing 23 compounds (monoterpenes: 90.4%; sesquiterpenes: 7.0%). The major component was 1,8-cineole (53.9%). This oil showed to be very different from those obtained from the same species. Larvae of A. aegypti were exposed to different concentrations of the essential oil and 1,8-cineole. The mortality rate of 100% was obtained after 24h of treatment with the oil at concentrations of 500 and 1,000 ppm. After 48h of treatment, the mortality rate was 80% and 50% for concentrations of 250 and 100 ppm, respectively. The LC50 obtained after 24h was estimated in 289.9 ppm and after 48h was 134.1 ppm. The major compound 1,8-cineole showed no larvicidal activity.

scholarly journals Larvicidal activity of microparticles of Melissa officinalis L. essential oil (Lamiaceae) against Aedes aegypti (Diptera, Culicidae)

2021 ◽  
Vol 10 (1) ◽  
pp. e35710111166
Author(s):  
Thércia Gabrielle Teixeira Martins ◽  
Paulo Victor Serra Rosa ◽  
Mariana Oliveira Arruda ◽  
Andressa Almeida Santana Dias ◽  
Ari Pereira de Araújo Neto ◽  
...  
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Fourth Instar ◽  
Tween 20 ◽  
Distilled Water ◽  
Melissa Officinalis ◽  
The Face ◽  
Muench Method

This study aimed to evaluate the larvicidal activity of essential oil microparticles (EO) of Melissa officinalis L. against Aedes aegypti. The leaves of M. officinalis were collected in the municipality of São José de Ribamar, Maranhão, Brazil, later dried, crushed and ground. 90g of the dried leaves were used to obtain the EO by the hydrodistillation method. For the synthesis of microencapsulated EO, 60g of sodium alginate (2.5% m/v) was added to the mixture of 15g of Tween 20 with 6g of EO. The mixture was homogenized and drips over CaCl2 5% m/v solution for the hardening of particles via crosslinking. The microparticles were washed with distilled water in filter and dried at 35ºC/24h and 15 days at tamb (30ºC). The eggs of Aedes aegypti were collected at the Federal University of Maranhão by the ovitrampas method. The larvae that hatched were fed until they reached the fourth instar. Groups of larvae (n=20) were submitted to solutions of EO and microparticles of 10-90 mg/L . After 24 h, live and dead larvae were counted and LC50 was calculated by the Reed&Muench method, using Cheng's criterion for classification of active potential. All larvae presented mortality in all concentrations tested. The LC50 obtained for the EO was 40.60 mg/L and for the microparticles 22.10 mg/L, both classified as active according to the adopted criterion, but it is observed that the microparticles increased the larvicidal potential of the EO. Through the results obtained, it is concluded that the microparticles formulated with the EO proved to be efficient in the face of the larvae of Aedes aegypti, being interesting and important in controlling and combating the mosquito that transmits dengue.

scholarly journals Tephrosia Toxicaria Pers Essential Oil: Chemical Composition and Larvicidal Activity

2006 ◽  
Vol 1 (5) ◽  
pp. 1934578X0600100 ◽  
Author(s):  
Walber H. F. Ribeiro ◽  
Jackson N. Vasconcelos ◽  
Angela M. C. Arriaga ◽  
Maria C. F. de Oliveira ◽  
Manoel Andrade-Neto ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Essential Oils ◽  
Larvicidal Activity ◽  
Mosquito Larvae ◽  
Main Constituent ◽  
Germacrene D

The essential oils obtained by hydrodistillation of the leaves, stems, and shoots of Tephrosia toxicaria Pers (Papilionaceae) were analyzed using GC and GC/MS. Germacrene D was the major component (20.7-33.3 %) of the oils of leaves and shoots, whereas spathulenol was the main constituent (42.3%) of the stem oil. The essential oils from the leaves and stems, when tested for activity against mosquito larvae (Aedes aegypti), gave LC 50 values of 81.3 ± 2.9 and 63.1 ± 1.0 ppm, respectively.

The Coherence of Mary Barton: Romance, Realism, and Utopia

2015 ◽  
Vol 5 (3) ◽  
pp. 185-200
Author(s):  
Robert Z. Birdwell
Keyword(s):  
Thomas Carlyle ◽  
The Novel ◽  
Second Stage ◽  
The Third ◽  
Third Stage ◽  
Three Stages ◽  
Mode Of Production

Critics have argued that Elizabeth Gaskell's first novel, Mary Barton (1848), is split by a conflict between the modes of realism and romance. But the conflict does not render the novel incoherent, because Gaskell surpasses both modes through a utopian narrative that breaks with the conflict of form and gives coherence to the whole novel. Gaskell not only depicts what Thomas Carlyle called the ‘Condition of England’ in her work but also develops, through three stages, the utopia that will redeem this condition. The first stage is romantic nostalgia, a backward glance at Eden from the countryside surrounding Manchester. The second stage occurs in Manchester, as Gaskell mixes romance with a realistic mode, tracing a utopian drive toward death. The third stage is the utopian break with romantic and realistic accounts of the Condition of England and with the inadequate preceding conceptions of utopia. This third stage transforms narrative modes and figures a new mode of production.

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