scholarly journals Chemical study and larvicidal activity against Aedes aegypti of essential oil of Piper aduncum L. (Piperaceae)

2013 ◽  
Vol 85 (4) ◽  
pp. 1227-1234 ◽  
Author(s):  
GISELE L. OLIVEIRA ◽  
SHEILA K. CARDOSO ◽  
CELIO R. LARA JUNIOR ◽  
THALLYTA M. VIEIRA ◽  
ELSIE F. GUIMARAES ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Mortality Rate ◽  
Essential Oil ◽  
Larvicidal Activity ◽  
Inflammatory Diseases ◽  
Folk Medicine ◽  
Chemical Study ◽  
Major Constituent ◽  
Flame Ionization ◽  
Piper Aduncum

ABSTRACT Piper aduncum L. is used in folk medicine to treat respiratory and inflammatory diseases. The aim of this study was to analyze the essential oil from leaves of P. aduncum collected in the Brazilian Cerrado, North of Minas Gerais, as well as to evaluate the larvicidal activity of this oil and of its major constituent. The essential oil was analyzed by gas chromatography coupled to flame ionization detector and gas chromatography coupled to mass spectrometry that allowed characterizing 23 compounds (monoterpenes: 90.4%; sesquiterpenes: 7.0%). The major component was 1,8-cineole (53.9%). This oil showed to be very different from those obtained from the same species. Larvae of A. aegypti were exposed to different concentrations of the essential oil and 1,8-cineole. The mortality rate of 100% was obtained after 24h of treatment with the oil at concentrations of 500 and 1,000 ppm. After 48h of treatment, the mortality rate was 80% and 50% for concentrations of 250 and 100 ppm, respectively. The LC50 obtained after 24h was estimated in 289.9 ppm and after 48h was 134.1 ppm. The major compound 1,8-cineole showed no larvicidal activity.

scholarly journals Chemical Constituents of the Essential Oil from Ecuadorian Endemic Species Croton ferrugineus and Its Antimicrobial, Antioxidant and α-Glucosidase Inhibitory Activity

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4608
Author(s):  
Eduardo Valarezo ◽  
Génesis Gaona-Granda ◽  
Vladimir Morocho ◽  
Luis Cartuche ◽  
James Calva ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Endemic Species ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Moderate Activity ◽  
Gram Positive Bacteria ◽  
Flame Ionization ◽  
Total Composition ◽  
Radical Scavenging Properties

Croton ferrugineus Kunth is an endemic species of Ecuador used in traditional medicine both for wound healing and as an antiseptic. In this study, fresh Croton ferrugineus leaves were collected and subjected to hydrodistillation for extraction of the essential oil. The chemical composition of the essential oil was determined by gas chromatography equipped with a flame ionization detector and gas chromatography coupled to a mass spectrometer using a non-polar and a polar chromatographic column. The antibacterial activity was assayed against three Gram-positive bacteria, one Gram-negative bacterium and one dermatophyte fungus. The radical scavenging properties of the essential oil was evaluated by means of DPPH and ABTS assays. The chemical analysis allowed us to identify thirty-five compounds representing more than 99.95% of the total composition. Aliphatic sesquiterpene hydrocarbon trans-caryophyllene was the main constituent with 20.47 ± 1.25%. Other main compounds were myrcene (11.47 ± 1.56%), β-phellandrene (10.55 ± 0.02%), germacrene D (7.60 ± 0.60%), and α-humulene (5.49 ± 0.38%). The essential oil from Croton ferrugineus presented moderate activity against Candida albicans (ATCC 10231) with an MIC of 1000 μg/mL, a scavenging capacity SC50 of 901 ± 20 µg/mL with the ABTS method, and very strong antiglucosidase activity with an IC50 of 146 ± 20 µg/mL.

scholarly journals Chemical Composition and Antimicrobial Activities of the Essential Oil From the Leaves of Pterocephalus hookeri

2020 ◽  
Vol 15 (12) ◽  
pp. 1934578X2098123
Author(s):  
Peng-fei Yang ◽  
Hui Lu ◽  
Qiong-bo Wang ◽  
Zhi-wei Zhao ◽  
Qiang Liu ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization ◽  
And Staphylococcus Aureus ◽  
Antimicrobial Assay ◽  
Detailed Chemical

Detailed chemical constituents of essential oil from the Pterocephalus hookeri leaves and its antimicrobial activities were investigated in this study. The essential oil, obtained by hydrodistillation, was characterized by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry analyses. Among the 90 identified compounds, hexadecanoic acid (21.27%), phytol (8.03%), furfural (7.08%), oleic acid (5.25%), and phytone (4.56%) were the major components. In the antimicrobial assay, the essential oil showed strong inhibitory activities against Escherichia coli, Candida albicans, and Staphylococcus aureus with minimum inhibitory concentration values of 31.3, 62.5, and 125 µg/mL, respectively. To our knowledge, this is the first report concerning chemical composition and antimicrobial activities of the essential oil from Pterocephalus hookeri.

scholarly journals Minerals, Essential Oils, and Biological Properties of Melissa officinalis L.

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1066
Author(s):  
Fahima Abdellatif ◽  
Muhammad Akram ◽  
Samir Begaa ◽  
Mohammed Messaoudi ◽  
Adel Benarfa ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
Phytopathogenic Fungi ◽  
Melissa Officinalis ◽  
Flame Ionization ◽  
Analysis Technique ◽  
Total Oil

This study describes the minerals elements, chemical composition, antioxidant and antimicrobial activities of Algerian Melissa officinalis plant. The essential oil (EO) was extracted by hydrodistillation (HD) using a Clevenger-type apparatus of dry leaves of M. officinalis and was analyzed by two techniques, gas chromatography coupled with flame ionization (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). Eighteen minerals comprising both macro- and microelements (As, Br, K, La, Na, Sb, Sm, Ba, Ca, Ce, Co, Cr, Cs, Fe, Rb, Sc, Th, and Zn) were determined using neutron activation analysis technique for the first time from Algerian Melissa officinalis plant. Seventy-eight compounds were identified in the essential oil, representing 94.090% of the total oil and the yields were 0.470%. The major component was geranial (45.060%). Other predominant components were neral (31.720%) and citronellal (6.420%). The essential oil presented high antimicrobial activity against microorganisms, mainly five human pathogenic bacteria, one yeast, Candida albicans, and two phytopathogenic fungi. The results can be used as a source of information for the pharmaceutical industry and medical research.

scholarly journals Antiprotozoal Activity of Turkish Origanum onites Essential Oil and Its Components

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4421 ◽  
Author(s):  
Tasdemir ◽  
Kaiser ◽  
Demirci ◽  
Demirci ◽  
Baser
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Mammalian Cells ◽  
Leishmania Donovani ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Flame Ionization ◽  
Main Component ◽  
Origanum Onites

Essential oil of Origanum species is well known for antimicrobial activity, but only a few have been evaluated in narrow spectrum antiprotozoal assays. Herein, we assessed the antiprotozoal potential of Turkish Origanum onites L. oil and its major constituents against a panel of parasitic protozoa. The essential oil was obtained by hydrodistillation from the dried herbal parts of O. onites and analyzed by Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography coupled with Mass Spectrometry (GC-MS). The in vitro activity of the oil and its major components were evaluated against Trypanosoma brucei rhodesiense, T. cruzi, Leishmania donovani, and Plasmodium falciparum. The main component of the oil was identified as carvacrol (70.6%), followed by linalool (9.7%), p-cymene (7%), γ-terpinene (2.1%), and thymol (1.8%). The oil showed significant in vitro activity against T. b. rhodesiense (IC50 180 ng/mL), and moderate antileishmanial and antiplasmodial effects, without toxicity to mammalian cells. Carvacrol, thymol, and 10 additional abundant oil constituents were tested against the same panel; carvacrol and thymol retained the oil’s in vitro antiparasitic potency. In the T. b. brucei mouse model, thymol, but not carvacrol, extended the mean survival of animals. This study indicates the potential of the essential oil of O. onites and its constituents in the treatment of protozoal infections.

scholarly journals Chemical composition of the essential oil of Psidium guajava leaves and its toxicity against Sclerotinia sclerotiorum

2018 ◽  
Vol 39 (2) ◽  
pp. 865
Author(s):  
Elizabeth Aparecida Josefi Silva ◽  
Vanessa Paula da Silva ◽  
Cassia Cristina Fernandes Alves ◽  
José Milton Alves ◽  
Edson Luiz Souchie ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Sclerotinia Sclerotiorum ◽  
Psidium Guajava ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization ◽  
Randomized Design ◽  
Dry Seasons ◽  
Guava Leaves

Globally, the fungus Sclerotinia sclerotiorum is considered as one of the most important phytopathogens, since it affects the production of several economically important crops. Further, it is difficult to control, thus increasing the use of pesticides. Therefore, the search for new substances, especially those extracted from plants, has received special attention to control this plant pathogen. The objective of the present study was to evaluate the yield and chemical composition of the essential oil extracted from the fresh leaves of guava collected during the rainy and dry seasons, and also to evaluate its toxicity against S. sclerotiorum. The experiment was a completely randomized design with five treatments and six replicates. The essential oil was extracted by hydrodistillation of the leaves and analyzed by gas chromatography-flame ionization detector and gas chromatography-mass spectrometry. The main compounds identified in the essential oil were trans-caryophyllene and ?-humulene. The essential oil at a concentration of 300 ?L exhibited 90% inhibition of the mycelial growth of S. sclerotiorum. This confirmed the antifungal potential of the essential oil of the guava leaves during both the sampling seasons.

scholarly journals Chemical Composition of Vernonia albicans Essential Oil from India

2014 ◽  
Vol 9 (7) ◽  
pp. 1934578X1400900
Author(s):  
Rajesh K. Joshi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Flame Ionization Detector ◽  
Ionization Detector ◽  
Flame Ionization ◽  
Aerial Parts ◽  
Sesquiterpene Hydrocarbons ◽  
Total Oil

The chemical composition of the hydro-distilled essential oil obtained from the flowering aerial parts of Vernonia albicans DC. (Asteraceae) was analyzed by gas chromatography equipped with a flame ionization detector (GC-FID) and gas chromatography coupled with a mass spectrometry (GC/MS). Thirty-nine compounds have been identified, representing 97.5% of the total oil. The major constituents were β-caryophyllene (34.3%), γ-amorphene (19.5%), 9-epi-β-caryophyllene (6.9%), and α-pinene (6.9%). The oil was found to be rich in sesquiterpene hydrocarbons (73.9%).

scholarly journals Comparison of Volatiles of Sideritis caesarea Specimens Collected from Different Localities in Turkey

2017 ◽  
Vol 12 (10) ◽  
pp. 1934578X1701201
Author(s):  
Tuğba Günbatan ◽  
Betül Demirci ◽  
İlhan Gürbüz ◽  
Fatih Demircib ◽  
Ayşe Mine Gençler Özkanc
Keyword(s):  
Gas Chromatography ◽  
Folk Medicine ◽  
Volatile Components ◽  
Chemical Compositions ◽  
Moderate Activity ◽  
Caryophyllene Oxide ◽  
Hexadecanoic Acid ◽  
Flame Ionization ◽  
Main Components

Sideritis caesarea H. Duman, Aytaç & Başer of the Lamiaceae is an Anatolian endemic species, and is mainly used as herbal tea and folk medicine. This present study aimed to determine and compare the chemical compositions of the volatile components of S. caesarea specimens collected from different localities in Kayseri province, Turkey: Sariz town (samples A and B), Pınarbaşı-Kaynar (sample C) and Pınarbaşı-Şirvan Mountain (sample D), respectively. Initially, the essential oils of the aerial parts were obtained by hydrodistillation and analysed both by gas chromatography-flame ionization detector (GC-FID), gas chromatography/mass spectrometer (GC/MS), simultaneously. Major volatile components of samples A and D were characterized as hexadecanoic acid (19.7 and 20.5%), caryophyllene oxide (6.7 and 20.2%), β-caryophyllene (6.5 and 12.6%), respectively. In sample B, hexadecanoic acid (14.6%), β-caryophyllene (11.5%) and caryophyllene oxide (8.3%) were detected as the major components. Whereas caryophyllene oxide (13.7%), hexadecanoic acid (8.5%) and spathulenol (6.1%) were the main components of the essential oil of sample C. Besides the chemical profiling, in vitro antimicrobial effects of samples were evaluated against a panel of six pathogenic microorganisms. Inhibition zones of the tested samples varied against bacteria with low to moderate activity, where no susceptibility against Candida albicans was observed.

scholarly journals Suppression of Linalool Acetate Production in Lavandula x intermedia

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Amy Desautels ◽  
Kamal Biswas ◽  
Alexander Lane ◽  
Astrid Boeckelmann ◽  
Soheil S. Mahmoud
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Essential Oils ◽  
Gas Chromatography Mass Spectrometry ◽  
Major Constituent ◽  
Improved Method ◽  
Acetate Production ◽  
Essential Oil Production ◽  
Sensitive Method

Linalool acetate, one of the major constituent of several essential oils, is heat-labile and decomposes upon exposure to the high injector temperature during gas chromatography. Here we report the development of an improved method for detection of this compound by gas chromatography mass spectrometry (GCMS) using cold on-column injection of the sample. By using this sensitive method, it has been demonstrated that a lavandin (L.x intermedia) mutant accumulates trace quantities of linalool acetate and camphor and higher amounts of cineole and borneol compared to its parent. This plant, which very likely carries a point mutation in one or more of the genes involved in essential oil production, provides a unique tool for investigating regulation of essential oil biogenesis in plants.

scholarly journals Essential Oil Constituents of Etlingera yunnanensis and Hornstedtia sanhan grown in Vietnam

2015 ◽  
Vol 10 (2) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Dao T. M. Chau ◽  
Do N. Dai ◽  
Tran M. Hoi ◽  
Tran H. Thai ◽  
Tran D. Thang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Ionization Detector ◽  
Methyl Chavicol ◽  
Flame Ionization ◽  
Germacrene D ◽  
Leaf Oil ◽  
Different Parts

The chemical composition of the essential oils obtained from the hydrodistillation of different parts of Etlingera yunnanensis (T.L. Wu &S.J. Chen) R.M. Sm. and Hornstedtia sanhan M.F. Newman (Zingiberaceae) grown in Vietnam are reported. The analysis was performed by means of a gas chromatography-flame ionization detector (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). The essential oil of the leaves of E. yunnanensis comprised mainly germacrene D (19.2%), β-pinene (11.6%), and α-amorphene (11.2%), while that of the stems was rich in β-pinene (23.7%), 1,8-cineole (11.0%) and α-pinene (9.6%). The major components of the root oil of E. yunnanensis were β-pinene (31.9%), α-pinene (13.7%) and 1,8-cineole (9.4%). However, α-pinene (25.9%, 36.3% and 14.1% for leaves, stems and roots, respectively), β-pinene (9.7%, 11.8% and 7.2% for leaves, stems and roots, respectively) and limonene (22.1%, 24.6% and 16.2% for leaves, stems and roots, respectively) were the major compounds of H. sanhan. In addition, α-humulene (15.9%) was present in larger amounts in the leaf oil while the root oil contained the largest proportion of methyl chavicol (43.7%).

scholarly journals First Report on Yield and Chemical Composition of Essential Oil Extracted from Myrcia eximia DC (Myrtaceae) from the Brazilian Amazon

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 783
Author(s):  
Oberdan Oliveira Ferreira ◽  
Jorddy Neves da Cruz ◽  
Celeste de Jesus Pereira Franco ◽  
Sebastião Gomes Silva ◽  
Wanessa Almeida da Costa ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Steam Distillation ◽  
Brazilian Amazon ◽  
Major Constituent ◽  
Chemical Compositions ◽  
Mass Yield ◽  
New Information ◽  
The City

The essential oil (EO) of plants of the Myrtaceae family has diverse chemical composition and several applications. However, data on the oil yield, its composition, and its complete chemistry are still unavailable for some species belonging to this family, such as Myrcia eximia DC. In this study, the chemical compositions of the EOs of Myrcia eximia were evaluated by using gas chromatography (GC) alone and gas chromatography coupled with mass spectrometry (GC–MS). Samples for both evaluations were collected from the city of Magalhães Barata, State of Pará, Brazil, in 2017 and 2018. For the plant material collected in 2017, EO was obtained by hydrodistillation (HD) only, while, for the material collected in 2018, EO was obtained by hydrodistillation and steam distillation (SD), in order to evaluate the differences in chemical composition and mass yield of the EO. The yields of (E)-caryophyllene were 15.71% and 20.0% for the samples collected by HD in 2017 and 2018, respectively, while the yield was 15.0% for the sample collected by SD in 2018. Hexanal was found to be the major constituent in the EO obtained by HD, with yield of up to 26.09%. The oil yields reached 0.08% by using SD, and 0.01% and 0.36% for the samples collected in 2017 and 2018, respectively, using HD. The results of this study provide new information about the mass yield and chemical composition of Myrcia eximia DC, and they can add value and income to traditional populations, as well as facilitate the preservation of this species.

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