scholarly journals Metabarcoding Cyanobacteria in coastal waters and sediment in central and southern Adriatic Sea

2020 ◽  
Vol 79 (2) ◽  
pp. 157-169 ◽  
Author(s):  
Anamarija Kolda ◽  
Zrinka Ljubešić ◽  
Ana Gavrilović ◽  
Jurica Jug-Dujaković ◽  
Kristina Pikelj ◽  
...  
Keyword(s):  
Water Column ◽  
Adriatic Sea ◽  
Anthropogenic Activities ◽  
Fish Fauna ◽  
Amplicon Sequencing ◽  
Chemical Parameters ◽  
16S Rrna Amplicon Sequencing ◽  
Physico Chemical ◽  
Indicator Group ◽  
Tropical Areas

Seasonal sampling of the seawater column and sediment in Adriatic coastal areas affected by various anthropogenic activities, primarily aquaculture, was conducted during 2017. In total, 32 samples from two sites (central and southern Adriatic) were analysed by 16S rRNA amplicon sequencing. This approach was selected to test the possibilities of using metabarcoding in studying marine cyanobacteria, exploring their ecology and potential as an indicator group in anthropologically stressed coastal environments. Additionally, physicochemical water column parameters, sediment granulometry and composition were assessed. Water column revealed a seasonal variation of amplicon sequencing variants (ASVs) closely related to Cyanobium PCC-6307, Prochlorococcus MIT9313 and Synechococcus CC9902, as well as seasonal grouping of physico-chemical parameters in PCA analysis. Sediment analysis uncovered greater community richness of 13 cyanobacterial genera and two uncultured groups. The most abundant in sandy gravels and gravelly sand type of sediments were ASVs closely related to Pleurocapsa PCC-7319 and Xenococcus PCC-7305. Furthermore, identified cyanobacterial ASVs predominantly displayed similarity to isolates from tropical areas (e.g. Neolyngbya, Chroococcidiopsis, Trichodesmium, etc.), which could indicate the tropicalization process already ongoing in the fish fauna of the Adriatic Sea.

scholarly journals Cyclical Patterns Affect Microbial Dynamics in the Water Basin of a Nuclear Research Reactor

2021 ◽  
Vol 12 ◽  
Author(s):  
Valérie Van Eesbeeck ◽  
Ruben Props ◽  
Mohamed Mysara ◽  
Pauline C. M. Petit ◽  
Corinne Rivasseau ◽  
...  
Keyword(s):  
Research Reactor ◽  
Nuclear Research ◽  
Amplicon Sequencing ◽  
Cell Number ◽  
Dead Cell ◽  
Chemical Parameters ◽  
Water Basin ◽  
16S Rrna Amplicon Sequencing ◽  
Physico Chemical ◽  
Nuclear Research Reactor

The BR2 nuclear research reactor in Mol, Belgium, runs in successive phases of operation (cycles) and shutdown, whereby a water basin surrounding the reactor vessel undergoes periodic changes in physico-chemical parameters such as flow rate, temperature, and radiation. The aim of this study was to explore the microbial community in this unique environment and to investigate its long-term dynamics using a 16S rRNA amplicon sequencing approach. Results from two sampling campaigns spanning several months showed a clear shift in community profiles: cycles were mostly dominated by two Operational Taxonomic Units (OTUs) assigned to unclassified Gammaproteobacterium and Pelomonas, whereas shutdowns were dominated by an OTU assigned to Methylobacterium. Although 1 year apart, both campaigns showed similar results, indicating that the system remained stable over this 2-year period. The community shifts were linked with changes in physico-chemical parameters by Non-metric Multidimensional Scaling (NMDS) and correlation analyses. In addition, radiation was hypothesized to cause a decrease in cell number, whereas temperature had the opposite effect. Chemoautotrophic use of H2 and dead cell recycling are proposed to be used as a strategies for nutrient retrieval in this extremely oligotrophic environment.

scholarly journals Prokaryotic Communities in the Thalassohaline Tuz Lake, Deep Zone, and Kayacik, Kaldirim and Yavsan Salterns (Turkey) Assessed by 16S rRNA Amplicon Sequencing

2021 ◽  
Vol 9 (7) ◽  
pp. 1525
Author(s):  
Can Akpolat ◽  
Ana Beatriz Fernández ◽  
Pinar Caglayan ◽  
Baris Calli ◽  
Meral Birbir ◽  
...  
Keyword(s):  
16S Rrna ◽  
Salt Lake ◽  
Amplicon Sequencing ◽  
Deep Zone ◽  
Biotechnological Potential ◽  
16S Rrna Amplicon Sequencing ◽  
Physico Chemical ◽  
Potential Applications ◽  
A Minor ◽  
Tuz Lake

Prokaryotic communities and physico-chemical characteristics of 30 brine samples from the thalassohaline Tuz Lake (Salt Lake), Deep Zone, Kayacik, Kaldirim, and Yavsan salterns (Turkey) were analyzed using 16S rRNA amplicon sequencing and standard methods, respectively. Archaea (98.41% of reads) was found to dominate in these habitats in contrast to the domain Bacteria (1.38% of reads). Representatives of the phylum Euryarchaeota were detected as the most predominant, while 59.48% and 1.32% of reads, respectively, were assigned to 18 archaeal genera, 19 bacterial genera, 10 archaeal genera, and one bacterial genus that were determined to be present, with more than 1% sequences in the samples. They were the archaeal genera Haloquadratum, Haloarcula, Halorhabdus, Natronomonas, Halosimplex, Halomicrobium, Halorubrum, Halonotius, Halolamina, Halobacterium, and Salinibacter within the domain Bacteria. The genera Haloquadratum and Halorhabdus were found in all sampling sites. While Haloquadratum, Haloarcula, and Halorhabdus were the most abundant genera, two uncultured Tuz Lake Halobacteria (TLHs) 1 and 2 were detected in high abundance, and an additional uncultured haloarchaeal TLH-3 was found as a minor abundant uncultured taxon. Their future isolation in pure culture would permit us to expand our knowledge on hypersaline thalassohaline habitats, as well as their ecological role and biomedical and biotechnological potential applications.

scholarly journals The character of the Bódva by the composition of the fishassociations

2007 ◽  
pp. 37-44
Author(s):  
Lajos Juhász
Keyword(s):  
Fish Species ◽  
Research Program ◽  
Food Source ◽  
Fish Fauna ◽  
Chemical Characteristics ◽  
Chemical Parameters ◽  
Protected Species ◽  
Physico Chemical ◽  
Hilly Region ◽  
Barbatula Barbatula

The Bódva is one of our rivers that flow outside the Hungarian borders and arrive to the country across the frontier.The Bódva is approximately 110 kilometres long, its upper course is mountaneous- like in its springfield in Slovakia and it reaches the Sajó by flowing among the lower hilly region. The river wall is ravine-like in more places, the river itself is devious, and the water basin is rather variable as in the underlay muddy segments and heavily shingly beds can also be found.By its fish fauna and physico-chemical characteristics, the Bódva is such aliving water that-after Borne and others (Nowicki, Thienemann) river zone system- can be regarded as an almost uniform typical Barbel zone.According to our research program (2001-2005) and literature resources we estimated the fish fauna of the Bódva on both the Slovakian and Hungarian course. From some four decades to the wide ecofaunistic examinations of the near past we surveyed the quondam and present fish species of the Bódva River. According to our survey some 40 species compose the recent fish fauna of this river.In the fish fauna of the Bódva those fish species that presently own a tight ecospectrum, sensitive to the changes of the environment thus so called indicator species, can also be found just like the less sensitive, invasive, aggressively reproducing species. By examining the changes in the fishfauna, the complex of those biogen and abiogen processes that affect the river can be indicated well. It can be stated that the fishfauna of the Bódva is rather rich with a countrywide measure also, and maintain a lot of rare, regressive protected and worth while protection species.In the last period of the construction, the racial and quantitive composition of the fishfauna of the upper course of the Bódva has changed significantly. Certain species (in the Hungarian course) has vanished almost totally or at least their number declined not ably. Among the vanishing species there are not only a few substantial, protected species of the fauna (e.g. Barbus pelononnesius petényi, Leucaspius delineatus, Zingel sterber, Barbatula barbatula), but the number of the once mass species also decreased appreciably (e.g. Rhodeus sericeus, Alburnoides bipunctatus, Cobitis elongatoides). With the above mentioned changes the number of other animals also decreased in the course that we examined (e.g. mussels, crabs). We examine the cause of these changes in the pollution of the river and according to that in the decrease of the food source and the negative changes of the physico-chemical parameters of the water.

scholarly journals Distribution and Diversity of Macrophytes in Relation to Some Physico-Chemical Factors in the Ketar River, Ziway Catchment, Ethiopia

2021 ◽  
Author(s):  
Yadesa Chibsa ◽  
Seyoum Mengistou ◽  
Demeke Kifle
Keyword(s):  
Relative Frequency ◽  
Nutrient Loading ◽  
Anthropogenic Activities ◽  
Pistia Stratiotes ◽  
Chemical Parameters ◽  
Physico Chemical ◽  
Significant Difference ◽  
Chemical Factors ◽  
Floating Macrophytes ◽  
Favorable Conditions

Abstract Distribution and diversity of macrophytes in relation to some physico-chemical factors in the Ketar River were studied from December, 2017 to November, 2018. Physico-chemical parameters and macrophytes were collected from three stations along the river for eight months. Onsite measurements and laboratory work of physico-chemical was analyzed as recommended by APHA [31]. Macrophytes were collected manually using belt transect method. Except for pH and surface water temperatures, all the physico-chemical parameters measured showed no significant difference spatially. During the study period, sixteen macrophyte species belonged to fourteen families were identified. Among the identified macrophyte, 11 of them were emergent, while 3 were rooted with floating leaves and 2 free-floating. Free-floating macrophytes were shared the highest abundance followed by emergent. This research observed that the site (site 3) that was exposed to minimal human impact was rich in diversity and abundance of macrophytes. All the sites were dominated by emergent macrophytes that attained the highest relative frequency followed by rooted emergent species. Azolla nilotica and Pistia stratiotes were shared the highest abundance and were the dominant macrophyte with the relative frequency of 7.24% and density of 40.91%, and 7.93% and 26.54%, respectively. Under a favorable environment, nutrient loading from nearby creates more favorable conditions for the infestation of the invasive species (A. nilotica and P. stratiotes) to flourish and out-compete the other species of macrophyte. Therefore, anthropogenic activities that enhance nutrient addition to the River should be regulated.

scholarly journals Assessment of Physico-Chemical Parameters of Soil of Muthannan Kulam Wetland, Coimbatore, Tamil Nadu, India

2014 ◽  
Vol 2 (3) ◽  
pp. 302-304 ◽  
Author(s):  
K Manimegalai ◽  
S Sukanya
Keyword(s):  
Electrical Conductivity ◽  
Physicochemical Parameters ◽  
Tamil Nadu ◽  
Anthropogenic Activities ◽  
Reliable Index ◽  
Chemical Parameters ◽  
Bottom Soil ◽  
Physico Chemical ◽  
Nitrogen Phosphorus

Soil serves as a more reliable index for productivity than water qualities. The productivity of any pond depends largely on the quality of bottom soil that is “store house of nutrients.” The present paper deals with the study of physicochemical parameters like organic carbon, pH, Electrical conductivity, Nitrogen, Phosphorus, Potassium of soil from Muthannan Kulam,Coimbatore (March 2013- May 2013). Analysis for the above parameters indicated contamination of the soil quality due to some anthropogenic activities. DOI: http://dx.doi.org/10.3126/ijasbt.v2i3.10769Int J Appl Sci Biotechnol, Vol. 2(3): 302-304  

Distribution of phytoplankton community in Kotor Bay (south-eastern Adriatic Sea)

2012 ◽  
Vol 7 (3) ◽  
pp. 470-486 ◽  
Author(s):  
Dragana Drakulović ◽  
Branka Pestorić ◽  
Mirko Cvijan ◽  
Slađana Krivokapić ◽  
Nenad Vuksanović
Keyword(s):  
Phytoplankton Community ◽  
Adriatic Sea ◽  
Sampling Period ◽  
Single Species ◽  
Chemical Parameters ◽  
The South ◽  
Phytoplankton Assemblages ◽  
South Eastern ◽  
Physico Chemical ◽  
Shallow Coastal Area

AbstractThe goal of this paper was to explain variability of phytoplankton in a shallow coastal area in relation to physico-chemical parameters. Temporal variability and composition of phytoplankton were investigated in the Kotor Bay, a small bay located in the south-eastern part of the Adriatic Sea. Samplings were performed weekly from February 2008 to January 2009 at one station in the inner part of the Kotor Bay, at five depths (0 m, 2 m, 5 m, 10 m, 15 m). Phosphates, nitrites and nitrates ranged from values under the level of detection to the maximum values of 1.54, 1.53 and 23.91 µmol l−1, respectively. The phytoplankton biomass — represented by chlorophyll a concentration — ranged from 0.12 to 6.78 mg m−3, reaching a maximum in summer. Diatoms were present throughout the whole sampling period, reaching the highest abundance in March (3.42×105 cells l−1at surface). The peak of dinoflagellates in July (2.2×106 cells l−1 at surface) was due to a single species, Prorocentrum micans. The toxic dinoflagellate Dinophysis fortii occurred at a concentration of 2140 cells l−1 in May. The present results of phytoplankton assemblages and distribution provide valuable information for this part of the south-eastern Adriatic Sea where data is currently absent.

scholarly journals Fish Diversity and their Limnological Status of River Yamuna at Kalpi, U.P., India

2016 ◽  
Vol 19 ◽  
pp. 57-62
Author(s):  
Manoj Kumar Shukla ◽  
Pushpendra Kumar Khare ◽  
Sadhana Chaurasia ◽  
Ravindra Singh
Keyword(s):  
Fish Fauna ◽  
Uttar Pradesh ◽  
Quality Analysis ◽  
Fish Diversity ◽  
Chemical Parameters ◽  
Yamuna River ◽  
Urban Centers ◽  
Physico Chemical ◽  
Survival And Growth ◽  
River Yamuna

We explore fish diversity and limnological status in the Yamuna River in Kalpi district, Uttar Pradesh, India. Several industrial and urban centers sit on the banks of the Yamuna in Uttar Pradesh. We selected four sampling stations on the Yamuna for purposes of fish fauna and water quality analysis over the course of a calendar year (October 2013 – September 2014). The samples were analyzed for selected physico-chemical analysis, and we recorded the types of fishes as well. We observed that most physico-chemical parameters were in a suitable range for the survival and growth of fish fauna. We recorded 29 species of fishes, belonging to 21 genera of 10 families.HYDRO Nepal JournalJournal of Water, Energy and EnvironmentIssue: 19Page: 57-62

scholarly journals Assessment of Some Physico-Chemical Parameters of Lagos Lagoon, Southwestern Nigeria

2019 ◽  
pp. 09-11
Author(s):  
Peter Olaoye Oyeleke ◽  
Samuel Olatunde Popoola ◽  
Olushola Ayoola Abiodun
Keyword(s):  
Dissolved Oxygen ◽  
Point Source ◽  
Anthropogenic Activities ◽  
Health And Safety ◽  
Chemical Parameters ◽  
Southwestern Nigeria ◽  
Mean Values ◽  
Physico Chemical ◽  
Significant Difference ◽  
Lagos Lagoon

The pollution status of Lagos Lagoon was investigated to determine some physical and chemical parameters, in order to further understand its water quality. Five locations were selected according to the anthropogenic activities taking place in the areas. The measured parameters are water temperature, salinity, conductivity, pH, dissolved oxygen (DO) and alkalinity. The mean values ranged from 28.50-30.15°C for temperature, 7.86-8.50 for pH, ND-10.60‰ for salinity, 0.18-15.20µS/cm for electrical conductivity, 12.00-14.00 mg/l for alkalinity and 1.60-6.40 mg/l for dissolved oxygen. The statistical analysis of the measured physicochemical parameters revealed non-significant difference (p>0.05) across all stations in the physico-chemical parameters. Generally, the values obtained suggest the influence of anthropogenic activities especially the depletion of DO in most of the stations. Therefore, point source and non-point source pollution into the Lagos Lagoon should be controlled for the health and safety of the aquatic ecosystems. Generally, all the measured parameters were either below or within the permissible limits obtainable in the marine environment.

Seasonal variation in water quality and fish diversity of Rampur Ghol, a wetland in Chitwan, Central Nepal

2013 ◽  
Vol 3 (1) ◽  
pp. 9-17
Author(s):  
Bam Bahadur Oli ◽  
Dilip Kumar Jha ◽  
Prakash Chandra Aryal ◽  
Madhav Kumar Shrestha ◽  
Dharma Raj Dangol ◽  
...  
Keyword(s):  
Water Quality ◽  
Seasonal Variation ◽  
Fish Fauna ◽  
Seasonal Fluctuation ◽  
Significant Decline ◽  
Fish Diversity ◽  
Diversity Analysis ◽  
Chemical Parameters ◽  
Central Nepal ◽  
Physico Chemical

The present study was carried out on Rampur Ghol in three seasons viz., monsoon, post-monsoon and pre-monsoon from September, 2012 to April, 2013 to assess the seasonal variation in physico-chemical parameters of water and fish diversity. The analysis of various physico-chemical parameters showed significant seasonal fluctuation in the water quality. However, the Ghol water was found to be suitable for fish fauna. Similarly, fish diversity analysis showed that Rampur Ghol is rich in fish fauna with 22 species belonging to 13 families and five orders. The study showed significant decline of fish species as well as their abundance from past studies.

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