scholarly journals Crocus adamioides (Iridaceae) in Bulgarian flora

2020 ◽  
Vol 80 (1) ◽  
Author(s):  
Tsvetanka Raycheva ◽  
Kiril Stoyanov ◽  
Samir Naimov ◽  
Elena Apostolova-Kuzova
Keyword(s):  
Internal Transcribed Spacer ◽  
Natural Populations ◽  
Type Specimen ◽  
Phylogenetic Position ◽  
Internal Transcribed Spacer Region ◽  
Separate Species ◽  
Bulgarian Population ◽  
First Time ◽  
Bayesian Phylogeny ◽  
Flora Of Bulgaria

Crocus adamioides Kernd. et Pasche, as recently known, was originally treated as C. biflorus Mill. subsp. adamii B. Mathew in the flora of Bulgaria by Mathew (1982) and verified by Rukšāns (2017). The taxon was afterwards described as a separate species (Kerndorff et al. 2012), with the holotype collected in Kırklareli Province, European Turkey. The species was for the first time mapped in two floristic regions of Bulgaria. The diagnostic characters based on the general morphology and leaf anatomy were defined from the natural populations of the species and compared to the type specimen and relevant data from the literature. The phylogenetic position of the species was clarified by sequencing of the internal transcribed spacer region (ITS: ITS1 + 5.SsrDNA + ITS2) and comparison of the obtained sequence with those annotated in NCBI. A phylogenetic tree was built using Bayesian phylogeny. Results have shown highest phylogenetic similarity with C. adamioides from Turkey. The closest relative C. ranjeloviciorum Kernd., Pasche, Harpke et Raca remains in proximity. Our morphological, anatomical and molecular analyses have revealed that the Bulgarian population shows a peculiar combination of characters specific to C. adamioides.

First report of Hericium cirrhatum from Pakistan

Mycotaxon ◽  
2020 ◽  
Vol 135 (4) ◽  
pp. 845-852
Author(s):  
Junaid Khan ◽  
Hassan Sher ◽  
Shah Hussain ◽  
Abdul Nasir Khalid
Keyword(s):  
Dna Sequence ◽  
Internal Transcribed Spacer ◽  
Morphological Characters ◽  
Sequence Analyses ◽  
Internal Transcribed Spacer Region ◽  
First Report ◽  
First Time

Hericium cirrhatum, a widespread but locally rare tooth fungus is reported for the first time from Pakistan. This species is characterized by white to cream semicircular basidiomata (usually arranged in tiers) with a granular to slightly spiny sterile upper surface, a fertile under surface with 10–15 mm long pointed spines, and ellipsoid to subglobose basidiospores. Morphological characters and DNA sequence analyses inferred from the nuclear ribosomal internal transcribed spacer region (nrITS) confirm the identity of the taxon.

A new Crocus L. (Iridaceae) species from SE Turkey, based on morphological and molecular data

Phytotaxa ◽  
2015 ◽  
Vol 239 (3) ◽  
pp. 223 ◽  
Author(s):  
Osman Erol ◽  
Doerte Harpke ◽  
Hasan Yıldırım
Keyword(s):  
Phylogenetic Analyses ◽  
Type Specimen ◽  
Morphological Characters ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
Phylogenetic Position ◽  
Full Description ◽  
New Taxon ◽  
Internal Transcribed Spacer Region ◽  
Se Turkey

Crocus musagecitii is described as a new species. Diagnostic morphological characters, a full description and detailed illustrations are provided on the basis of the type specimen and wild specimens. Morphologically, C. musagecitii is close to Crocus biflorus subsp. pseudonubigena. Crocus musagecitii differs from C. biflorus subsp. pseudonubigena by the lack of stripes or narrow purplish tongue on outside of outer tepals, wider tepals, and homogenously yellow anthers. In order to clarify the phylogenetic position of this species within the Crocus adamii species complex, we sequenced the internal transcribed spacer region (ITS: ITS1 + 5.8SrDNA + ITS2) and 5’ external transcribed spacer (ETS) of the nuclear ribosomal DNA (rDNA). A phylogenetic tree obtained by Bayesian phylogenetic inference is given. Phylogenetic analyses revealed that the new taxon is close to C. munzurensis. Crocus musagecitii differs from its phylogenetically closest relative C. munzurensis by the corm tunics (C. musagecitii: coriaceus; C. munzurensis: membranous), the number of leaves (C. musagecitii: up to 8; C. munzurensis: up to 4) and non-hairy leaf margins.

scholarly journals Diversity of Diatrypaceae Species Associated with Dieback of Grapevines in South Africa, with the Description of Eutypa cremea sp. nov.

Plant Disease ◽  
2018 ◽  
Vol 102 (1) ◽  
pp. 220-230 ◽  
Author(s):  
Providence Moyo ◽  
Lizel Mostert ◽  
Christoffel F.J. Spies ◽  
Ulrike Damm ◽  
Francois Halleen
Keyword(s):  
South Africa ◽  
Cross Section ◽  
Internal Transcribed Spacer ◽  
Dominant Species ◽  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
Internal Transcribed Spacer Region ◽  
Canker Pathogen ◽  
Grapevine Canker ◽  
First Time

Recent studies in grape-growing areas including Australia, California, and Spain have revealed an extensive diversity of Diatrypaceae species on grapevines showing dieback symptoms and cankers. However, in South Africa, little is known regarding the diversity of these species in vineyards. The aim of this study was, therefore, to identify and characterize Diatrypaceae species associated with dieback symptoms of grapevine in South Africa. Isolates were collected from dying spurs of grapevines aged 4 to 8 years old, grapevine wood showing wedge-shaped necrosis when cut in cross section as well as from perithecia on dead grapevine wood. The collected isolates were identified based on morphological characters and phylogenetic analyses of the internal transcribed spacer region (ITS) and β-tubulin gene. Seven Diatrypaceae species were identified on grapevine, namely Cryptovalsa ampelina, C. rabenhorstii, Eutypa consobrina, E. lata, E. cremea sp. nov., Eutypella citricola, and E. microtheca. The dying spurs yielded the highest diversity of species when compared with the wedge-shaped necrosis and/or perithecia. C. ampelina was the dominant species in the dying spurs, followed by E. citricola, whereas E. lata was the dominant species isolated from the wedge-shaped necroses and perithecia. These results confirm E. lata as an important grapevine canker pathogen in South Africa, but the frequent association of C. ampelina with spur dieback suggests that this pathogen plays a more prominent role in dieback than previously assumed. In some cases, more than one species were isolated from a single symptom, which suggests that interactions may be occurring leading to decline of grapevines. C. rabenhorstii, E. consobrina, E. citricola, E. microtheca, and E. cremea are reported for the first time on grapevine in South Africa.

Taxonomy, biogeography and phylogenetic position of the North African Ceramius maroccanus-complex (Hymenoptera: Vespidae, Masarinae)

1999 ◽  
Vol 30 (3) ◽  
pp. 323-348
Author(s):  
Volker Mauss
Keyword(s):  
Sister Group ◽  
Species Group ◽  
Phylogenetic Position ◽  
Flight Period ◽  
North African ◽  
Separate Species ◽  
The North ◽  
As Species ◽  
The Status ◽  
First Time

AbstractThe Ceramius maroccanus-complex is endemic to southwestern Morocco. The status of C. maroccanus (Giordani Soika 1957) and C. montanus Gusenleitner 1990 as separate species is confirmed, C. rubripes Gusenleitner 1990 stat. n. and C. gessi sp. n. are recognized as species for the first time. The four species are redescribed/described and illustrated, and their distribution and flight period are analysed. Identification keys to males and females are provided. The C. maroccanus-complex is a monophyletic group within 'Species group 7' of Richards 1962, and is probably the sister group of the C. lusitanicus-complex.

Kamalia indica, a new genus and species belonging to Diaporthales (Ascomycota, Fungi) described based on morphology and molecular phylogeny

Phytotaxa ◽  
2019 ◽  
Vol 425 (4) ◽  
pp. 233-243
Author(s):  
SHIWALI RANA ◽  
SANJAY KUMAR SINGH
Keyword(s):  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
New Genus ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Type Specimen ◽  
Morphological Description ◽  
Fungal Culture ◽  
Internal Transcribed Spacer Region

A fungal taxa isolated from leaf spots of Mallotus philippensis from Kangra district of North-Western Himalayan region of India is established as a new genus based on morphological characters of asexual-morphs, cultural characteristics and phylogenetic analyses of the partial nuclear ribosomal 28S large subunit (LSU) and internal transcribed spacer (ITS) rDNA sequence data. The generic placement of the genus has been determined based on DNA sequences from authenticated isolates. The present taxon has turned out to be distinct, showing nearly 90% identity with other known genera in Diaporthales based on nrDNA internal transcribed spacer region. The morphological description is provided for the new taxa and compared with the similar taxa belonging to the order Diaporthales. The culture was found to show heavy sporulation in all kind of media. The type specimen and ex type culture have been deposited in the Ajrekar Mycological Herbarium (AMH) and National Fungal Culture Collection of India (NFCCI-WDCM 932), respectively.

The European species of Chalarus Walker, 1834 revisited (Diptera: Pipunculidae)

Zootaxa ◽  
2008 ◽  
Vol 1936 (1) ◽  
pp. 1-39 ◽  
Author(s):  
CHRISTIAN KEHLMAIER ◽  
THORSTEN ASSMANN
Keyword(s):  
Cytochrome Oxidase ◽  
Internal Transcribed Spacer ◽  
Dna Barcodes ◽  
Cytochrome Oxidase Subunit ◽  
Internal Transcribed Spacer Region ◽  
Male And Female ◽  
European Species ◽  
Males And Females ◽  
Second Internal Transcribed Spacer ◽  
First Time

The European representatives of the pipunculid genus Chalarus are taxonomically revised. Chalarus absconditus Kehlmaier spec. nov., C. immanis Kehlmaier spec. nov., C. marki Kehlmaier spec. nov. and C. saxonicus Kehlmaier spec. nov. are described as new to science. Males of C. pughi Coe, 1966, C. clarus Jervis, 1992 and C. longicaudis Jervis, 1992 are characterized for the first time, as is the female for C. griseus Coe, 1966. A lectotype is designated for Chalarus velutinus Macquart, 1834 and the name is proposed for synonymy with C. spurius (Fallén, 1816). Chalarus parmenteri Coe, 1966 and C. perplexus Jervis, 1992 are proposed as junior synonyms of C. holosericeus (Meigen, 1824). The assignment of males and females is established through DNA barcodes, using fragments of the mitochondrial coding gene cytochrome oxidase subunit I (coxI) and nuclear Second Internal Transcribed Spacer region (ITS2). An identification key to the European representatives of the genus is provided, including figures of male and female terminalia for most species.

scholarly journals Phylogenetic Analysis of Cercospora and Mycosphaerella Based on the Internal Transcribed Spacer Region of Ribosomal DNA

2001 ◽  
Vol 91 (7) ◽  
pp. 648-658 ◽  
Author(s):  
Stephen B. Goodwin ◽  
Larry D. Dunkle ◽  
Victoria L. Zismann
Keyword(s):  
Phylogenetic Analysis ◽  
Internal Transcribed Spacer ◽  
Large Scale ◽  
Plant Pathogens ◽  
Sequence Data ◽  
Gray Leaf Spot ◽  
Its Sequences ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
Separate Species

Most of the 3,000 named species in the genus Cercospora have no known sexual stage, although a Mycosphaerella teleomorph has been identified for a few. Mycosphaerella is an extremely large and important genus of plant pathogens, with more than 1,800 named species and at least 43 associated anamorph genera. The goal of this research was to perform a large-scale phylogenetic analysis to test hypotheses about the past evolutionary history of Cercospora and Mycosphaerella. Based on the phylogenetic analysis of internal transcribed spacer (ITS) sequence data (ITS1, 5.8S rRNA gene, ITS2), the genus Mycosphaerella is monophyletic. In contrast, many anamorph genera within Mycosphaerella were polyphyletic and were not useful for grouping species. One exception was Cercospora, which formed a highly supported monophyletic group. Most Cercospora species from cereal crops formed a subgroup within the main Cercospora cluster. Only species within the Cercospora cluster produced the toxin cercosporin, suggesting that the ability to produce this compound had a single evolutionary origin. Intraspecific variation for 25 taxa in the Mycosphaerella clade averaged 1.7 nucleotides (nts) in the ITS region. Thus, isolates with ITS sequences that differ by two or more nucleotides may be distinct species. ITS sequences of groups I and II of the gray leaf spot pathogen Cercospora zeae-maydis differed by 7 nts and clearly represent different species. There were 6.5 nt differences on average between the ITS sequences of the sorghum pathogen Cercospora sorghi and the maize pathogen Cercospora sorghi var. maydis, indicating that the latter is a separate species and not simply a variety of Cercospora sorghi. The large monophyletic Mycosphaerella cluster contained a number of anamorph genera with no known teleomorph associations. Therefore, the number of anamorph genera related to Mycosphaerella may be much larger than suspected previously.

Dwindling status of Epimedium Elatum (Morren & Decne) and its geographical distribution in Kashmir Himalaya, India

2018 ◽  
pp. 47-52
Keyword(s):  
Medicinal Plant ◽  
Biological Activities ◽  
Conservation Status ◽  
Natural Populations ◽  
Culture Techniques ◽  
Kashmir Himalayas ◽  
Near Future ◽  
First Time ◽  
Tissue Culture Techniques

Epimedium elatum (Morren & Decne) of family Berberidaceace is a rare perennial medicinal plant, endemic to high altitude forests of Northwestern Himalayas in India. Ethnobotanically, it has been used as an ingredient for treatment of bone-joint disorders, impotence and kidney disorders in Kashmir Himalayas. Phytochemically, it is rich in Epimedin ABC and Icariin; all of these have been demonstrated to possess remarkable biological activities like PDE-5 inhibition (treatment of erectile dysfunction), anticancer, antiosteoporosis antioxidant and antiviral properties. The present investigation reports its traditional usage, comprehensive distribution and conservation status from twenty ecogeographical regions in Kashmir Himalayas, India. The species was reported from Gurez valley for the first time. Numerous threats like excessive grazing, deforestration, habitat fragmentation, tourism encroachment, landslides and excessive exploitation have decreased its natural populations in most of the surveyed habitats. Consequently, its existence may become threatened in near future if timely conservation steps are not taken immediately by concerned stakeholders involved in medicinal plant research. Moreover, use of plant tissue culture techniques is recommended for development of its in vitro propagation protocols. Therefore, introduction of this medicinal plant in botanical gardens, protected sites and development of monitoring programmes are needed for its immediate conservation in Northwestern Himalayas, India.

Hemiflagellochloris (Chlorophyceae, Chlorophyta), a new for Russian flora genus of green algae

2014 ◽  
Vol 48 ◽  
pp. 104-113
Author(s):  
A. D. Temraleeva ◽  
E. V. Mincheva ◽  
Yu. S. Bukin ◽  
M. V. Eltsov ◽  
V. A. Demkin ◽  
...  
Keyword(s):  
Green Algae ◽  
Molecular Analysis ◽  
18S Rdna ◽  
Phylogenetic Position ◽  
Rdna Gene ◽  
Algal Strain ◽  
The North ◽  
Volgograd Region ◽  
First Time ◽  
18S Rdna Gene

The genus Hemiflagellochloris Watanabe et al. was found in Russia for the first time. The algal strain was isolated from a buried solonetz in the zone of dry steppes (the north-end of Ergeni Hills, Volgograd Region). The morphology and ecology of this isolate are described. Its phylogenetic position within Chlorophyceae is determined by molecular analysis of 18S rDNA gene.

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