Antimicrobial Activity of Trifoliate Orange (Poncirus trifoliate) Seed Extracts on Gram-Negative Food-borne Pathogens

ABSTRACT Food-borne pathogens are responsible for most cases of food poisoning in developed countries and are often associated with poultry products, including chicken. Little is known about the role of β-defensins in the chicken digestive tract and their efficacy. In this study, the expression of chicken β-defensin gallinacin-6 (Gal-6) and its antimicrobial activity against food-borne pathogens were investigated. Reverse transcription-PCR analysis showed high expression of Gal-6 mRNA in the esophagus and crop, moderate expression in the glandular stomach, and low expression throughout the intestinal tract. Putative transcription factor binding sites for nuclear factor kappa beta, activator protein 1, and nuclear factor interleukin-6 were found in the Gal-6 gene upstream region, which suggests a possible inducible nature of the Gal-6 gene. In colony-counting assays, strong bactericidal and fungicidal activity was observed, including bactericidal activity against food-borne pathogens Campylobacter jejuni, Salmonella enterica serovar Typhimurium, Clostridium perfringens, and Escherichia coli. Treatment with 16 μg/ml synthetic Gal-6 resulted in a 3 log unit reduction in Clostridium perfringens survival within 60 min, indicating fast killing kinetics. Transmission electron microscopy examination of synthetic-Gal-6-treated Clostridium perfringens cells showed dose-dependent changes in morphology after 30 min, including intracellular granulation, cytoplasm retraction, irregular septum formation in dividing cells, and cell lysis. The high expression in the proximal digestive tract and broad antimicrobial activity suggest that chicken β-defensin gallinacin-6 plays an important role in chicken innate host defense.

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Antimicrobial activity of essential oil from Indonesian medicinal plants against food-borne pathogens

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/207/1/012036 ◽

2018 ◽

Vol 207 ◽

pp. 012036 ◽

Cited By ~ 1

Author(s):

T Budiati ◽

W Suryaningsih ◽

S. Umaroh ◽

B. Poerwanto ◽

A Bakri ◽

...

Keyword(s):

Antimicrobial Activity ◽

Medicinal Plants ◽

Essential Oil ◽

Food Borne Pathogens ◽

Food Borne

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Antimicrobial activity of thyme essential oil nanoemulsions on spoilage bacteria of fish and food-borne pathogens

Food Bioscience ◽

10.1016/j.fbio.2020.100635 ◽

2020 ◽

Vol 36 ◽

pp. 100635 ◽

Cited By ~ 6

Author(s):

Yesim Ozogul ◽

Esmeray Kuley Boğa ◽

Ismail Akyol ◽

Mustafa Durmus ◽

Yılmaz Ucar ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Food Borne Pathogens ◽

Thyme Essential Oil ◽

Food Borne ◽

Spoilage Bacteria

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Antimicrobial activity of select edible plants from Odisha, India against food-borne pathogens

LWT ◽

10.1016/j.lwt.2019.06.013 ◽

2019 ◽

Vol 113 ◽

pp. 108246 ◽

Cited By ~ 5

Author(s):

Sujogya Kumar Panda ◽

Yugal Kishore Mohanta ◽

Laxmipriya Padhi ◽

Walter Luyten

Keyword(s):

Antimicrobial Activity ◽

Edible Plants ◽

Food Borne Pathogens ◽

Food Borne

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In vitro antimicrobial activity of Muntingia calabura fruit extracts against food borne pathogens

Pharmacognosy Journal ◽

10.1016/j.phcgj.2013.05.005 ◽

2013 ◽

Vol 5 (3) ◽

pp. 135-136 ◽

Cited By ~ 1

Author(s):

G. Sibi ◽

Kalpana Kaushik ◽

Kalegowda Dhananjaya ◽

Kodagahally R. Ravikumar ◽

Honnaiah Mallesha

Keyword(s):

Antimicrobial Activity ◽

In Vitro Antimicrobial Activity ◽

Fruit Extracts ◽

Food Borne Pathogens ◽

Food Borne ◽

Muntingia Calabura

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Comparison of antibacterial effect of seven 1-monoglycerides on food-borne pathogens or spoilage bacteria

Acta Veterinaria Brno ◽

10.2754/avb201180010029 ◽

2011 ◽

Vol 80 (1) ◽

pp. 29-39 ◽

Cited By ~ 10

Author(s):

Leona Buňková ◽

František Buňka ◽

Rahula Janiš ◽

Jiří Krejčí ◽

Iva Doležálková ◽

...

Keyword(s):

Fatty Acids ◽

Growth Rate ◽

Specific Growth ◽

Lag Time ◽

Gram Negative Bacteria ◽

Inhibitory Effects ◽

Gram Negative ◽

Food Borne Pathogens ◽

Food Borne

The aim of this study was to compare under the same conditionsin vitrothe inhibitory effects of seven 1-monoglycerides (MAG) containing fatty acids with a medium chain on ten strains of food-borne pathogens or spoilage gram-positive and gram-negative bacteria (Bacillus cereus, Bacillus subtilis, Enterococcus faecalis, Micrococcus luteus, Staphylococcus aureus, Citrobacter freundii, Escherichia coli, Proteus mirabilis, Salmonella entericaser.Enteritidis andPseudomonas aeruginosa) and on their growth indicatos. The inhibitory effect of MAGs (monocaprylin, monocaprin, monolaurin, monomyristin, monopalmitin, MAG of undecanoic and 10-undecenoic acids) at a concentration of 25 – 1500 mg·l-1was observed. Growth of bacteria in the presence of MAG was studied by means of optical density of bacteria for 24 h. The data were modelled through a Gompertz equation and the lag-time, the maximum specific growth rate and the maximal value reached were calculated. MAGs inhibited mainly the growth of gram-positive bacteria, which was shown by the extended lag-time, decrease in specific growth rate and decrease in cell density. Inhibitory effects of tested MAGs could be ranked from point of view of the minimum inhibitory concentration: MAG-C12:0 > MAG-C11:0 > MAG-C10:0 > MAG-C14:0 > MAG-C11:1 > MAG-C8:0 > MAG-C16:0.In vitro, no significant inhibitory effects of 1-monoglycerides, with the exception of the highest concentrations applied, on the growth of gram-negative bacteria were detected. The main contribution of this study is to compare the effects of several MAG containing fatty acids with a medium chain under the same conditions on the growth indicators of bacteria.

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