Endogenous Markers for Estimation of Renal Function in Peritoneal Dialysis Patients

ObjectiveThis method comparison study, conducted at the peritoneal dialysis (PD) outpatient clinic of the Department of Renal Medicine, Aarhus University Hospital, Denmark, set out to evaluate the accuracy and reproducibility of methods for estimating glomerular filtration rate (GFR) based on endogenous markers in PD patients.PatientsThe 12 consecutive patients included in the study were examined twice while in a stable condition. All patients finished the study. Inclusion criteria were age 18 years or older, ability to collect 24-hour urine, and urine production greater than 300 mL in 24 hours.Main Outcome MeasuresThe methods for estimating GFR using endogenous markers included the average of urinary clearances of creatinine and urea [U-Cl(crea–urea)] and two equations using the serum concentration of cystatin C [eGFR(CysC)]. The resulting GFR estimates were compared with those obtained using urinary and corrected plasma clearances of51Cr-EDTA [U-Cl(EDTA) and cP-Cl(EDTA)], the corrected plasma clearance being plasma clearance minus dialysate clearance.ResultsCompared with the U-Cl(EDTA), the U-Cl(crea–urea) GFR estimate was 12% higher [95% confidence limits (CL): 3%, 21%]. Although significantly different ( p = 0.01), the latter two methods showed the best agreement. The estimates obtained using the eGFR(CysC) methods were skewed from y = x compared with the estimates obtained using other methods, indicating strong bias, probably because of extrarenal elimination. The cP-Cl(EDTA) estimate was 34% (95% CL: 26%, 42%), higher than the U-Cl(EDTA) estimate ( p < 0.001). The reproducibility (coefficients of variation) differed significantly between methods: cP-Cl(EDTA), 7%; U-Cl(EDTA), 14%; U-Cl(crea–urea), 18%; and both eGFR(CysC) methods, 3%.ConclusionsIn PD patients, GFR may be estimated as U-Cl(crea–urea) when complete urine collection is performed, taking into account an overestimation of approximately 12%. The available equations for eGFR(CysC) seem to be inaccurate; further development and validation is desirable. Omitting the eGFR(CysC) methods, cP-Cl(EDTA) was the most reproducible method and might be useful in certain situations.

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Verification study of free light chains assays on reagent-optimized analysers

Biochemia Medica ◽

10.11613/bm.2019.030709 ◽

2019 ◽

Vol 29 (3) ◽

pp. 579-586

Author(s):

Dragana Šegulja ◽

Danica Matišić ◽

Karmela Barišić ◽

Dunja Rogić

Keyword(s):

Method Comparison ◽

Correlation Coefficients ◽

Light Chains ◽

Free Light Chains ◽

Serum Samples ◽

Three Samples ◽

Coefficients Of Variation ◽

Satisfactory Precision ◽

Haematological Patients ◽

Method Comparison Study

Introduction: Our aim was to compare analytical specifications of two assays (monoclonal vs. polyclonal) for free light chains (FLCs) quantification optimized for two different analytical platforms, nephelometer ProSpec (Siemens, Erlangen, Germany) and turbidimetric analyser Optilite (The Binding Site, Birmingham, UK). Materials and methods: The evaluation included verification of the precision, repeatability and reproducibility, estimation of accuracy and method comparison study with 37 serum samples of haematological patients. Kappa and lambda FLC were measured in each sample by both methods and kappa/lambda ratio was calculated. Results: Results show satisfactory precision of both methods with coefficients of variation for ProSpec of CVwr = 2.20% and CVbr = 3.44%, and for Optilite CVwr = 2.82% and CVbr = 4.15%. Estimated bias for FLC lambda was higher on the ProSpec analyser, but bias for FLC kappa was higher on the Optilite analyser. Correlation coefficients were 0.98; P < 0.001 for FLC kappa and 0.97; P < 0.001 for FLC lambda. Considering normal/pathological FLC ratio moderate agreement within assays was detected (κ = 0.621). When the results were categorized according to criteria for progressive disease, 4/37 (0.10) cases were differently classified. Lambda FLC values by Optilite in three samples with monoclonal FLC lambda were more than twelve times higher than by ProSpec. A 25% difference in FLC ratio was detected in 16/37 (0.43) and 50% difference in 13/37 (0.35) patients. Conclusions: All manufacturers’ precision claims could not be achieved in the verification study. The comparison of results to biological variations data showed that coefficients of variations are acceptable for both assays. The assays should not be used interchangeably in haematological patients.

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Preview Rapid Strep A Test Method Comparison Study

Case Medical Research ◽

10.31525/ct1-nct03907449 ◽

2019 ◽

Author(s):

Keyword(s):

Method Comparison ◽

Test Method ◽

Comparison Study ◽

Method Comparison Study

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Evaluation of point‐of‐care testing device for anemia detection: A cross‐sectional method comparison study from Thailand

Journal of Clinical Laboratory Analysis ◽

10.1002/jcla.23976 ◽

2021 ◽

Author(s):

Wittawat Chantkran ◽

Pitipat Jamnarnwej ◽

Pipat Sritanabutr ◽

Pasra Arnutti

Keyword(s):

Point Of Care ◽

Method Comparison ◽

Testing Device ◽

Comparison Study ◽

Point Of Care Testing ◽

Cross Sectional ◽

Method Comparison Study

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Development and Validation of 2-Azaspiro [4,5] Decan-3-One (Impurity A) in Gabapentin Determination Method Using qNMR Spectroscopy

Molecules ◽

10.3390/molecules26061656 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1656

Author(s):

Nataliya E. Kuz’mina ◽

Sergey V. Moiseev ◽

Mikhail D. Khorolskiy ◽

Anna I. Lutceva

Keyword(s):

Test Procedure ◽

Active Pharmaceutical Ingredient ◽

Test Methods ◽

Intermediate Precision ◽

Coefficients Of Variation ◽

Validation Parameters ◽

Limit Of Quantitation ◽

Student’S T ◽

Development And Validation ◽

Student’S T Test

The authors developed a 1H qNMR test procedure for identification and quantification of impurity A present in gabapentin active pharmaceutical ingredient (API) and gabapentin products. The validation studies helped to determine the limit of quantitation and assess linearity, accuracy, repeatability, intermediate precision, specificity, and robustness of the procedure. Spike-and-recovery assays were used to calculate standard deviations, coefficients of variation, confidence intervals, bias, Fisher’s F test, and Student’s t-test for assay results. The obtained statistical values satisfy the acceptance criteria for the validation parameters. The authors compared the results of impurity A quantification in gabapentin APIs and capsules by using the 1H qNMR and HPLC test methods.

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Results of Peritoneal Equilibration Test during Treatment with Polyglucose Dialysis Solution

Peritoneal Dialysis International ◽

10.1177/089686080202200310 ◽

2002 ◽

Vol 22 (3) ◽

pp. 357-364 ◽

Cited By ~ 1

Author(s):

Alicja E. Grzegorzewska ◽

Danuta Antczak-Jȩdrzejczak ◽

Magdalena Leander

Keyword(s):

Peritoneal Dialysis ◽

Dwell Time ◽

Control Period ◽

University Hospital ◽

Control Group ◽

Peritoneal Equilibration Test ◽

Dialysis Unit ◽

Dialysis Solution ◽

Glucose Ratio ◽

Peritoneal Permeability

Background Results of peritoneal equilibration test (PET) suggest prolonged effect of polyglucose dialysis solution (PG-DS) on peritoneal permeability. Objectives An evaluation of dialysate-to-plasma ratio (D/P) of urea, D/P creatinine, and D/D0 glucose (ratio of dialysate glucose at designated dwell time to dialysate glucose at 0 dwell time), and mass transfer area coefficients (KBD) of these solutes in PET before introduction, during administration, and after discontinuation of PG-DS in patients treated with continuous ambulatory peritoneal dialysis (CAPD). Design Single-center prospective study with PG-DS; retrospective selection of the control group. Setting Peritoneal dialysis unit in a university hospital. Patients Fourteen patients (11 males; age 45.1 ± 8.5 years) treated with CAPD for 17.5 ± 9.9 months. 7.5% PG-DS was used for the overnight exchange. After discontinuation of the PG-DS, standard dialysis solutions, as previously used, were reintroduced. The control group was selected to match both CAPD duration and peritoneal permeability of the patients in the PG-DS group at the start of the study. Methods Standard PET was carried out at 1.6 ± 0.8 months before the introduction of PG-DS (study period I, n = 14), after 1.2 ± 0.6 months’ use of PG-DS (study period II, n = 14), after 4.4 ± 0.8 months’ use of PG-DS (study period III, n = 11), after 8.8 ± 2.2 months’ use of PG-DS (study period IV, n = 9), and at 2.0 ± 0.6 months after PG-DS discontinuation (study period V, n = 11). Patients in the control group underwent PET at similar time intervals (control periods I – V). Results In the PG-DS group, a tendency toward increased peritoneal permeability for urea and creatinine was shown during the consecutive study periods. D/D0 glucose was significantly higher only in the PET performed during use of PG-DS (periods II – IV) compared to results obtained in period I. In the control group, both D/P and KBD of both urea and creatinine remained unchanged, but KBD glucose was higher in the first 2 hours of the PET in control period V compared to respective values in control period III. Conclusion Changes in peritoneal permeability are observed in CAPD patients treated with PG-DS. These changes may be at least partially related to the administration of polyglucose.

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Optimization of PV array inclination in India using ANN estimator: Method comparison study

Sadhana ◽

10.1007/s12046-015-0386-2 ◽

2015 ◽

Vol 40 (5) ◽

pp. 1457-1472 ◽

Cited By ~ 6

Author(s):

T V DIXIT ◽

ANAMIKA YADAV ◽

S GUPTA

Keyword(s):

Method Comparison ◽

Comparison Study ◽

Pv Array ◽

Method Comparison Study

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Comparison of Outcome of one Versus two Drains Insertion for Seroma Formation Following Modified Radical Mastectomy in Breast Carcinoma

Annals of PIMS-Shaheed Zulfiqar Ali Bhutto Medical University ◽

10.48036/apims.v17i1.499 ◽

2021 ◽

Vol 17 (1) ◽

pp. 52-55

Author(s):

Shiraz Shaikh ◽

Ambreen Munir ◽

Shahnawaz Abro ◽

Shahida Khatoon ◽

Zameer Hussain Laghari ◽

...

Keyword(s):

Breast Carcinoma ◽

General Surgery ◽

Stable Condition ◽

Radical Mastectomy ◽

Seroma Formation ◽

University Hospital ◽

P Value ◽

Modified Radical Mastectomy ◽

Group A ◽

Group B

Objective: Comparative outcome of one versus two drains insertion for in the term of seroma formation following modified radical mastectomy in breast carcinoma. Methodology: This Prospective Interventional trial was conducted at Department of General Surgery, Liaquat University Hospital Hyderabad from February 2018 to January 2019. Females with breast carcinoma admitted for modified radical mastectomy were included. Patients were divided into two groups. Groups I underwent one drain placement and group II underwent two drains placement. All patients were observed to measure and record the volume of the fluid. Patients were discharged from Hospital in stable condition and after removal of drains, and followed up weekly for one month. Data was recorded on self-made proforma and analyzed by using SPSS-20. Results: Total of 80 patients were selected, 38 in group A and 42 in group B. Mean age of patients of group A was 49.08 ± 9.89 years and group B was 51.40 ± 13.59 years. , Excised Mass weight was lesser in group A as compared to group B. Mean volume of drain discharge was significantly higher in Group B 323.43 ± 158.88 ml, while it was in group A 230.29± 200.98, findings were statistically significant 0.013. Seroma formation was statistically insignificant among both groups as 8(21.1%) in group A and 10(23.8%) in group B, p-value 0.768. Conclusion: One-drain and two-drain insertion are equally effective to reduce the seroma formation after modified radical mastectomy; however, one drain insertion leads to more patient compliance and comfort with probably less morbidity and cost.

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Association of B-Type Natriuretic Peptide Level with Residual Kidney Function in Incident Peritoneal Dialysis Patients

Peritoneal Dialysis International ◽

10.3747/pdi.2017.00241 ◽

2019 ◽

Vol 39 (2) ◽

pp. 147-154 ◽

Cited By ~ 1

Author(s):

Yasuhiro Kawai ◽

Shigeru Tanaka ◽

Hisako Yoshida ◽

Masatoshi Hara ◽

Hiroaki Tsujikawa ◽

...

Keyword(s):

Peritoneal Dialysis ◽

Kidney Function ◽

Natriuretic Peptide ◽

Subgroup Analysis ◽

Proportional Hazards Model ◽

Urine Volume ◽

Cox Proportional Hazards Model ◽

University Hospital ◽

Residual Kidney Function ◽

Increased Risk

Background Residual kidney function (RKF) is an important factor influencing both technique and patient survival in peritoneal dialysis (PD) patients. B-type natriuretic peptide (BNP) is considered a marker of cardio-renal syndrome. The relationship between BNP and RKF in PD patients remains unclear. Methods We conducted a prospective study of 89 patients who had started and continued PD for 6 months or more in Kyushu University Hospital between June 2006 and September 2015. Participants were divided into low BNP (≤ 102.1 ng/L) and high BNP (> 102.1 ng/L) groups according to median plasma BNP level at PD initiation. The primary outcome was RKF loss, defined as 24-hour urine volume less than 100 mL. We estimated the association between BNP and RKF loss using a Kaplan-Meier method and Cox proportional hazards model and compared the rate of RKF decline between the 2 groups. To evaluate the consistency of the association, we performed subgroup analysis stratified by baseline characteristics. Results During the median follow-up of 30 months, 30 patients lost RKF. Participants in the high BNP group had a 5.87-fold increased risk for RKF loss compared with the low BNP group after adjustment for clinical and cardiac parameters. A high plasma BNP level was more clearly associated with RKF loss in younger participants compared with older participants in subgroup analysis. Conclusions B-type natriuretic peptide may be a useful risk marker for RKF loss in PD patients. The clinical importance of plasma BNP level as a marker of RKF loss might be affected by age.

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Exercise Capacity and Association with Quality of Life in Peritoneal Dialysis Patients

Peritoneal Dialysis International ◽

10.3747/pdi.2018.00075 ◽

2019 ◽

Vol 39 (1) ◽

pp. 66-73 ◽

Cited By ~ 8

Author(s):

Kiyotaka Uchiyama ◽

Naoki Washida ◽

Kaori Muraoka ◽

Kohkichi Morimoto ◽

Takahiro Kasai ◽

...

Keyword(s):

Quality Of Life ◽

Peritoneal Dialysis ◽

Exercise Capacity ◽

Exercise Tolerance ◽

Short Form ◽

Handgrip Strength ◽

Quadriceps Strength ◽

University Hospital ◽

Skeletal Mass

Background Muscle wasting, common and progressive in uremic patients, is associated with a high probability for morbidity, lower health-related quality of life (HRQOL), and mortality. However, exercise tolerance in peritoneal dialysis (PD) patients has not been fully elucidated. The aim of this study was to evaluate exercise capacity, its determinants, and its association with HRQOL in PD patients. Methods Outpatients treated with PD at Keio University Hospital from December 2016 to March 2018 were included in this single-center cross-sectional observational study. Exercise capacity was assessed by incremental shuttle walking test (ISWT) and handgrip and quadriceps strength. In addition to evaluation of PD-related parameters, HRQOL was assessed by the Kidney Disease Quality of Life-Short Form questionnaire. Results Among the 50 recruited PD outpatients, age and PD vintage were 63.8 ± 9.6 and 3.8 ± 2.8 years, respectively. Physical examination revealed ISWT of 312.0 ± 138.2 m, handgrip strength of 27.5 ± 6.9 kg, and quadriceps strength of 23.3 ± 10.0 kg. Multivariate analysis showed that younger age and male sex were significantly associated with higher ISWT and handgrip and quadriceps strength. Skeletal mass index (SMI) remained a significant predictor of handgrip and quadriceps strength. Moreover, only ISWT was strongly correlated with higher HRQOL scores, including physical, mental, and kidney-specific domains, even after adjustment for age and sex. Conclusions Exercise tolerance in PD patients was partially determined by age, sex, and SMI. Moreover, this is the first study to demonstrate the strong relationship between aerobic capacity and HRQOL in PD patients.

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