Fusarium incarnatum-equiseti complex from China

The Fusarium incarnatum-equiseti species complex (FIESC) is shown to encompass 33 phylogenetic species, across a wide range of habitats/hosts around the world. Here, 77 pathogenic and endophytic FIESC strains collected from China were studied to investigate the phylogenetic relationships within FIESC, based on a polyphasic approach combining morphological characters, multi-locus phylogeny and distribution patterns. The importance of standardised cultural methods to the identification and classification of taxa in the FIESC is highlighted. Morphological features of macroconidia, including the shape, size and septum number, were considered as diagnostic characters within the FIESC. A multi-locus dataset encompassing the 5.8S nuclear ribosomal gene with the two flanking internal transcribed spacers (ITS), translation elongation factor (EF-1α), calmodulin (CAM), partial RNA polymerase largest subunit (RPB1) and partial RNA polymerase second largest subunit (RPB2), was generated to distinguish species within the FIESC. Nine novel species were identified and described. The RPB2 locus is demonstrated to be a primary barcode with high success rate in amplification, and to have the best species delimitation compared to the other four tested loci.

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A new species of Ophiocordyceps (Ophiocordycipitaceae) from Mizoram, India

Phytotaxa ◽

10.11646/phytotaxa.500.1.2 ◽

2021 ◽

Vol 500 (1) ◽

pp. 11-20

Author(s):

ZOHMANGAIHA CHAWNGTHU ◽

JOSIAH MC VABEIKHOKHEI ◽

RITA ZOMUANPUII ◽

SURAJIT DE MANDAL ◽

JOHN ZOTHANZAMA

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Elongation Factor ◽

Morphological Characters ◽

Internal Transcribed Spacers ◽

Paper Wasp ◽

Molecular Phylogenetic Analysis ◽

Translation Elongation ◽

Molecular Phylogenetic ◽

A New Species

Ophiocordyceps mizoramensisis described as a new species from Mizoram by using a combination of morphological and molecular phylogenetic analysis. The molecular phylogenetic analysis include both the subunits (LSU and SSU), the internal transcribed spacers (ITS1 and ITS2) with the 5.8S gene region, along with Tef (Translation elongation factor). The new species Ophiocordyceps mizoramensis, differs from other closely related species based on molecular phylogenetic relationships, genetic distance, and minor morphological characters related to sexual structures. The entomopathogenic Ophiocordyceps mizoramensis infected the host Polistes olivaceus (paper wasp) causing death to the whole colony of the hive.

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Identification and Characterization of Diaporthe ambigua, D. australafricana, D. novem, and D. rudis Causing a Postharvest Fruit Rot in Kiwifruit

Plant Disease ◽

10.1094/pdis-10-16-1535-re ◽

2017 ◽

Vol 101 (8) ◽

pp. 1402-1410 ◽

Cited By ~ 6

Author(s):

Gonzalo A. Díaz ◽

Bernardo A. Latorre ◽

Mauricio Lolas ◽

Enrique Ferrada ◽

Paulina Naranjo ◽

...

Keyword(s):

San Francisco ◽

Plant Pathogens ◽

Elongation Factor ◽

Its Region ◽

Fruit Rot ◽

Lesion Length ◽

Translation Elongation ◽

Wide Range ◽

Postharvest Rot ◽

Diaporthe Ambigua

Diaporthe spp. are important plant pathogens causing wood cankers, blight, dieback, and fruit rot in a wide range of hosts. During surveys conducted during the 2013 and 2014 seasons, a postharvest rot in Hayward kiwifruit (Actinidia deliciosa) was observed in Chile. In order to identify the species of Diaporthe associated with this fruit rot, symptomatic fruit were collected from seven kiwifruit packinghouses located between San Francisco de Mostazal and Curicó (central Chile). Twenty-four isolates of Diaporthe spp. were identified from infected fruit based on morphological and cultural characters and analyses of nucleotides sequences of three loci, including the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2), a partial sequences of the β-tubulin, and translation elongation factor 1-α genes. The Diaporthe spp. identified were Diaporthe ambigua, D. australafricana, D. novem, and D. rudis. Multilocus phylogenetic analysis revealed that Chilean isolates were grouped in separate clades with their correspondent ex-types species. All species of Diaporthe were pathogenic on wounded kiwifruit after 30 days at 0°C under normal and controlled-atmosphere (2% O2 and 5% CO2) storage and they were sensitive to benomyl, pyraclostrobin, and tebuconazole fungicides. D. ambigua isolates were the most virulent based on the lesion length measured in inoculated Hayward and Jintao kiwifruit. These findings confirm D. ambigua, D. australafricana, D. novem, and D. rudis as the causal agents of kiwifruit rot during cold storage in Chile. The specie D. actinidiae, a common of Diaporthe sp. found associated with kiwifruit rot, was not identified in the present study.

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Spegazzinia camelliae sp. nov. (Didymosphaeriaceae, Pleosprales), a new endophytic fungus from northern Thailand

Phytotaxa ◽

10.11646/phytotaxa.483.2.4 ◽

2021 ◽

Vol 483 (2) ◽

pp. 117-128

Author(s):

NAKARIN SUWANNARACH ◽

JATURONG KUMLA ◽

SAISAMORN LUMYONG

Keyword(s):

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Small Subunit ◽

Internal Transcribed Spacers ◽

Nuclear Ribosomal Dna ◽

Full Description ◽

Translation Elongation ◽

Spine Length ◽

Elongation Factor 1 Alpha

A new endophytic ascomycete, described herein as Spegazzinia camelliae, was isolated from leaves of Camellia sinensis var. assamica collected from Nan Province, Thailand. This species is characterized by basauxic conidiophores and dark brown to blackish brown α and β conidia. It can be distinguished from previously described Spegazzinia species by the spine length of the α conidia and the size of the β conidia. Multi-gene phylogenetic analyses of the small subunit (SSU), large subunit (LSU) and internal transcribed spacers (ITS) of the nuclear ribosomal DNA (rDNA) and the translation elongation factor 1-alpha (tef1) genes also support S. camelliae is a distinct new species within Spegazzinia. A full description, color photographs, illustrations and a phylogenetic tree showing the position of S. camelliae are provided.

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Morphological characters and phylogenetic analysis reveal a new species of Phellinus with hooked hymenial setae from Vietnam

Phytotaxa ◽

10.11646/phytotaxa.356.1.8 ◽

2018 ◽

Vol 356 (1) ◽

pp. 91 ◽

Cited By ~ 1

Author(s):

LIN ZHU ◽

XING JI ◽

JING SI ◽

BAO-KAI CUI

Keyword(s):

Rna Polymerase Ii ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characters ◽

Molecular Data ◽

Translation Elongation ◽

The Status ◽

Large Subunit Rdna ◽

Elongation Factor 1 ◽

A New Species

Phellinus vietnamensis sp. nov. is described from Vietnam based on morphological characters and molecular data. Morphologically, it is characterized by perennial, pileate basidiomata, a dimitic hyphal system, hooked hymenial setae, and colorless, broadly subglobose to ovoid, thick-walled basidiospores 5.5–6 × 4.8–5.2 μm. Phylogenetically, the status of Phellinus vietnamensis is strongly supported based on sequences of the nuclear internal transcribed spacer (ITS) regions, the translation elongation factor 1-α gene (EF1-α) nuclear large subunit rDNA (nrLSU) and the second largest subunits of RNA polymerase II (RPB2).

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Isolation, Molecular Identification and Mycotoxin Profile of Fusarium Species Isolated from Maize Kernels in Iran

Toxins ◽

10.3390/toxins11050297 ◽

2019 ◽

Vol 11 (5) ◽

pp. 297 ◽

Cited By ~ 4

Author(s):

Maryam Fallahi ◽

Hossein Saremi ◽

Mohammad Javan-Nikkhah ◽

Stefania Somma ◽

Miriam Haidukowski ◽

...

Keyword(s):

Fungal Pathogens ◽

Fusarium Species ◽

Animal Health ◽

Elongation Factor ◽

Ear Rot ◽

Translation Elongation ◽

Wide Range ◽

Severe Reduction ◽

Maize Kernels ◽

High Possibility

Fusarium species are among the most important fungal pathogens of maize, where they cause severe reduction of yield and accumulation of a wide range of harmful mycotoxins in the kernels. In order to identify the Fusarium species and their mycotoxin profiles associated to maize ear rot and kernel contamination in Iran, a wide sampling was carried out from field in ten major maize-producing provinces in Iran, during 2015 and 2016. From 182 samples of maize kernels, 551 strains were isolated and identified as belonging to Fusarium genus. Among the 234 representative strains identified at species level by translation elongation factor (EF-1α) sequences, the main Fusarium species were F. verticillioides and F. proliferatum, together representing 90% of the Iranian Fusarium population, and, to a lesser extent, F. incarnatum equiseti species complex (FIESC), F. thapsinum and F. redolens. Fumonisin (FBs) production by F. verticillioides and F. proliferatum representative strains was analysed, showing that all strains produced FB1. None of F. verticillioides strains produced FB2 nor FB3, while both FB2 and FB3 were produced only by F. proliferatum. Total mean of FBs production by F. verticillioides was higher than F. proliferatum. The occurrence of different Fusarium species on Iranian maize is reason of great concern because of the toxigenic risk associated to these species. Moreover, the diversity of the species identified increases the toxigenic risk associated to Fusarium contaminated maize kernels, because of the high possibility that a multi-toxin contamination can occur with harmful consequences on human and animal health.

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A new species of Heimioporus (Boletaceae) from southern China

Phytotaxa ◽

10.11646/phytotaxa.415.4.2 ◽

2019 ◽

Vol 415 (4) ◽

pp. 179-188

Author(s):

XIANG-NYU CHEN ◽

MING ZHANG ◽

TAI-HUI LI ◽

NIAN-KAI ZENG

Keyword(s):

New Species ◽

Southern China ◽

Phylogenetic Analyses ◽

Elongation Factor ◽

Morphological Characters ◽

Molecular Data ◽

Translation Elongation ◽

Distinct Member ◽

Elongation Factor 1 ◽

A New Species

Heimioporus sinensis, collected from tropical and subtropical areas of China, is introduced as a new species based on both morphological characters and molecular data. The species is characterized by the purplish red to deep magenta pileus, the reticulated stipe, the irregularly reticulate to reticulate-alveolate basidiospores 11.5–13.5 × 8–9.5 μm, and a trichodermal to intricately trichodermal pileipellis. Phylogenetic analyses based on the nuc 28S rDNA D1-D2 domains (28S) and the translation elongation factor 1-α gene (tef1-α) showed that H. sinensis is a distinct member of the genus Heimioporus in the subfamily Xerocomoideae.

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Multi-gene phylogeny and taxonomy of Amauroderma s. lat. (Ganodermataceae)

Persoonia - Molecular Phylogeny and Evolution of Fungi ◽

10.3767/persoonia.2020.44.08 ◽

2020 ◽

Vol 44 (1) ◽

pp. 206-239 ◽

Cited By ~ 3

Author(s):

Y.-F. Sun ◽

D.H. Costa-Rezende ◽

J.-H. Xing ◽

J.-L. Zhou ◽

B. Zhang ◽

...

Keyword(s):

Rna Polymerase ◽

Rna Polymerase Ii ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Examination ◽

Translation Elongation ◽

Multiple Loci ◽

Phylogenetic Studies ◽

Molecular Phylogenetic

Amauroderma s.lat. has been defined mainly by the morphological features of non-truncate and double-walled basidiospores with a distinctly ornamented endospore wall. In this work, taxonomic and phylogenetic studies on species of Amauroderma s.lat. are carried out by morphological examination together with ultrastructural observations, and molecular phylogenetic analyses of multiple loci including the internal transcribed spacer regions (ITS), the large subunit of nuclear ribosomal RNA gene (nLSU), the largest subunit of RNA polymerase II (RPB1) and the second largest subunit of RNA polymerase II (RPB2), the translation elongation factor 1-α gene (TEF) and the β-tubulin gene (TUB). The results demonstrate that species of Ganodermataceae formed ten clades. Species previously placed in Amauroderma s.lat. are divided into four clades: Amauroderma s.str., Foraminispora, Furtadoa and a new genus Sanguinoderma. The classification of Amauroderma s. lat. is thus revised, six new species are described and illustrated, and eight new combinations are proposed. SEM micrographs of basidiospores of Foraminispora and Sanguinoderma are provided, and the importance of SEM in delimitation of taxa in this study is briefly discussed. Keys to species of Amauroderma s.str., Foraminispora, Furtadoa, and Sanguinoderma are also provided.

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Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization inIris. lacteavar.chinensisRoots under Cadmium, Lead, and Salt Stress Conditions

The Scientific World JOURNAL ◽

10.1155/2014/532713 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Chun-Sun Gu ◽

Liang-qin Liu ◽

Chen Xu ◽

Yan-hai Zhao ◽

Xu-dong Zhu ◽

...

Keyword(s):

Gene Expression ◽

Salt Stress ◽

Real Time ◽

Reference Genes ◽

Elongation Factor ◽

Stress Conditions ◽

Translation Initiation Factor ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Wide Range

Quantitative real time PCR (RT-qPCR) has emerged as an accurate and sensitive method to measure the gene expression. However, obtaining reliable result depends on the selection of reference genes which normalize differences among samples. In this study, we assessed the expression stability of seven reference genes, namely, ubiquitin-protein ligase UBC9 (UBC), tubulin alpha-5 (TUBLIN), eukaryotic translation initiation factor (EIF-5A), translation elongation factor EF1A (EF1α), translation elongation factor EF1B (EF1b), actin11 (ACTIN), and histone H3 (HIS), inIris. lacteavar.chinensis(I. lacteavar.chinensis) root when the plants were subjected to cadmium (Cd), lead (Pb), and salt stress conditions. All seven reference genes showed a relatively wide range of threshold cycles (Ct) values in different samples. GeNorm and NormFinder algorithms were used to assess the suitable reference genes. The results from the two software units showed thatEIF-5AandUBCwere the most stable reference genes across all of the tested samples, whileTUBLINwas unsuitable as internal controls.I. lacteavar.chinensisis tolerant to Cd, Pb, and salt. Our results will benefit future research on gene expression in response to the three abiotic stresses.

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Characterization of Botryosphaeriaceae Species as Causal Agents of Trunk Diseases on Grapevines

Plant Disease ◽

10.1094/pdis-03-15-0286-re ◽

2015 ◽

Vol 99 (12) ◽

pp. 1678-1688 ◽

Cited By ~ 16

Author(s):

Antonia Carlucci ◽

Francesca Cibelli ◽

Francesco Lops ◽

Maria Luisa Raimondo

Keyword(s):

Elongation Factor ◽

Phylogenetic Study ◽

Translation Elongation ◽

Neofusicoccum Parvum ◽

Grapevine Trunk Diseases ◽

General Decline ◽

Wide Range ◽

Trunk Diseases ◽

Pathogenicity Tests

Botryosphaeriaceae spp. have a cosmopolitan distribution and a wide range of plant hosts. Over the last 15 years, worldwide, 21 species of this family have been associated with grapevine trunk diseases that cause cankers and dieback on grapevines. Here, we surveyed vineyards of Vitis vinifera ‘Lambrusco’, ‘Sangiovese’, and ‘Montepulciano’ in three areas of the Foggia province (Cerignola, Foggia, and San Severo) in southern Italy. Wood samples from grapevines showing general decline, dieback, cankers, and wood and foliar discoloration yielded 344 fungal isolates identified as Botryosphaeriaceae spp. A phylogenetic study combining internal transcribed spacer and translation elongation factor 1-α sequences of 60 representative isolates identified nine botryosphaeriaceous species: Botryosphaeria dothidea, Diplodia corticola, D. mutila, D. seriata, Dothiorella iberica, Do. sarmentorum, Lasiodiplodia citricola, L. theobromae, and Neofusicoccum parvum. Pathogenicity tests confirmed that all nine species cause canker and dieback of grapevines. However, this is the first report of L. citricola as causal agent of wood cankers and dieback of grapevine. To date, including L. citricola, there are 25 botryosphaeriaceous species associated with V. vinifera worldwide, of which 12 have been reported for grapevines in Italy.

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