CHARACTERISTICS OF MIRNA BINDING SITES IN MRNAS OF MYB GENES OF ARABIDOPSIS, SOYA AND GRAPE

2021 ◽  
Vol 1 (19) ◽  
pp. 205-206
Author(s):  
I.V. Pinskiy
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Glycine Max ◽  
Vitis Vinifera ◽  
Binding Sites ◽  
Myb Transcription Factor ◽  
Transcription Factor Genes ◽  
Myb Genes

The characteristics of various miRNA binding sites in the mRNAs of the MYB transcription factor genes of Arabidopsis thaliana, Glycine max and Vitis vinifera have been established. The most conserved miRNA binding sites were the binding sites of the miR828 family.

scholarly journals A single nucleotide polymorphism in an R2R3 MYB transcription factor gene triggers the male sterility in soybean ms6 (Ames1)

2021 ◽  
Author(s):  
Junping Yu ◽  
Guolong Zhao ◽  
Wei Li ◽  
Ying Zhang ◽  
Peng Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Glycine Max ◽  
Male Sterility ◽  
Bulked Segregant Analysis ◽  
Soybean Protein ◽  
Anther Development ◽  
Hybrid Breeding ◽  
Myb Transcription Factor ◽  
Male Sterile ◽  
R2r3 Myb

Abstract Key message Identification and functional analysis of the male sterile gene MS6 in Glycine max. Abstract Soybean (Glycine max (L.) Merr.) is an important crop providing vegetable oil and protein. The male sterility-based hybrid breeding is a promising method for improving soybean yield to meet the globally growing demand. In this research, we identified a soybean genic male sterile locus, MS6, by combining the bulked segregant analysis sequencing method and the map-based cloning technology. MS6, highly expressed in anther, encodes an R2R3 MYB transcription factor (GmTDF1-1) that is homologous to Tapetal Development and Function 1, a key factor for anther development in Arabidopsis and rice. In male sterile ms6 (Ames1), the mutant allele contains a missense mutation, leading to the 76th leucine substituted by histidine in the DNA binding domain of GmTDF1-1. The expression of soybean MS6 under the control of the AtTDF1 promoter could rescue the male sterility of attdf1 but ms6 could not. Additionally, ms6 overexpression in wild-type Arabidopsis did not affect anther development. These results evidence that GmTDF1-1 is a functional TDF1 homolog and L76H disrupts its function. Notably, GmTDF1-1 shows 92% sequence identity with another soybean protein termed as GmTDF1-2, whose active expression also restored the fertility of attdf1. However, GmTDF1-2 is constitutively expressed at a very low level in soybean, and therefore, not able to compensate for the MS6 deficiency. Analysis of the TDF1-involved anther development regulatory pathway showed that expressions of the genes downstream of TDF1 are significantly suppressed in ms6, unveiling that GmTDF1-1 is a core transcription factor regulating soybean anther development.

Identification and Functional Characterization of R3 MYB Transcription Factor Genes in Soybean

2018 ◽  
Vol 61 (2) ◽  
pp. 85-96 ◽  
Author(s):  
Hongwei Xun ◽  
Zhibing Zhang ◽  
Yunxiao Zhou ◽  
Xueyan Qian ◽  
Yingshan Dong ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Functional Characterization ◽  
Myb Transcription Factor ◽  
Transcription Factor Genes

Developmentally distinct MYB genes encode functionally equivalent proteins in Arabidopsis

Development ◽  
2001 ◽  
Vol 128 (9) ◽  
pp. 1539-1546 ◽  
Author(s):  
M.M. Lee ◽  
J. Schiefelbein
Keyword(s):  
Functional Relationship ◽  
Expression Patterns ◽  
Cell Types ◽  
Myb Transcription Factor ◽  
Regulatory Sequences ◽  
Myb Gene ◽  
Distinct Cell ◽  
Transcription Factor Genes ◽  
Myb Genes ◽  
Multicellular Organisms

The duplication and divergence of developmental control genes is thought to have driven morphological diversification during the evolution of multicellular organisms. To examine the molecular basis of this process, we analyzed the functional relationship between two paralogous MYB transcription factor genes, WEREWOLF (WER) and GLABROUS1 (GL1), in Arabidopsis. The WER and GL1 genes specify distinct cell types and exhibit non-overlapping expression patterns during Arabidopsis development. Nevertheless, reciprocal complementation experiments with a series of gene fusions showed that WER and GL1 encode functionally equivalent proteins, and their unique roles in plant development are entirely due to differences in their cis-regulatory sequences. Similar experiments with a distantly related MYB gene (MYB2) showed that its product cannot functionally substitute for WER or GL1. Furthermore, an analysis of the WER and GL1 proteins shows that conserved sequences correspond to specific functional domains. These results provide new insights into the evolution of the MYB gene family in Arabidopsis, and, more generally, they demonstrate that novel developmental gene function may arise solely by the modification of cis-regulatory sequences.

Ectopic expression of a R2R3 MYB transcription factor of dove tree (Davidia involucrata) aggravates seed abortion in Arabidopsis thaliana

2020 ◽  
Vol 47 (5) ◽  
pp. 454
Author(s):  
Jian Li ◽  
Tian Chen ◽  
Fengzhen Huang ◽  
Penghui Dai ◽  
Fuxiang Cao ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Transgenic Plants ◽  
Ectopic Expression ◽  
Myb Transcription Factor ◽  
Seed Abortion ◽  
Transgenic Seeds ◽  
Genes Encoding ◽  
Colour Changes ◽  
R2r3 Myb

Serious seed abortion of dove tree (Davidia involucrate Baill.) is one of the critical factors leading to the low fecundity of this species. Seed abortion is a complicated process and various factors have been verified to synergistically determine the fate of seeds. To reveal the mechanism of seed abortion in D. involucrata, we performed transcriptome analysis in normal and abortive seeds of D. involucrata. According to the transcriptome data, we noticed that most of the genes encoding a MYB transcription factor were predominantly expressed in abortive seeds. Among these, a gene named DiMYB1 was selected and its function was validated in this study. Overexpression of DiMYB1 resulted in obviously reduced viability of transgenic seeds and seedlings, and caused a significantly higher seed abortion rate. The vegetative growth of transgenic plants was hindered, resulting in an earlier flowering time. In addition, colour changes occurred in transgenic plants. Some transgenic sprouts, stems and pods appeared purple instead of green in colour. Our finding demonstrated that DiMYB1 participates in multiple plant developmental processes, especially in seed development in Arabidopsis thaliana (L.) Heynh., which indicated the similar role of this gene in D. involucrata.

MYB 56 Encoding a R2R3 MYB Transcription Factor Regulates Seed Size in Arabidopsis thaliana

2013 ◽  
Vol 55 (11) ◽  
pp. 1166-1178 ◽  
Author(s):  
Yanjie Zhang ◽  
Wanqi Liang ◽  
Jianxin Shi ◽  
Jie Xu ◽  
Dabing Zhang
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Seed Size ◽  
Myb Transcription Factor ◽  
R2r3 Myb

scholarly journals A R2R3-MYB Transcription Factor, VvMYBC2L2, Functions as a Transcriptional Repressor of Anthocyanin Biosynthesis in Grapevine (Vitis vinifera L.)

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 92 ◽  
Author(s):  
Ziguo Zhu ◽  
Guirong Li ◽  
Li Liu ◽  
Qingtian Zhang ◽  
Zhen Han ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Vitis Vinifera ◽  
Anthocyanin Biosynthesis ◽  
Transcriptional Repressor ◽  
Negative Regulator ◽  
Myb Transcription Factor ◽  
Vitis Vinifera L ◽  
Flavonoid Pathway ◽  
Leucoanthocyanidin Reductase ◽  
R2r3 Myb

In grapevine, the MYB transcription factors play an important role in the flavonoid pathway. Here, a R2R3-MYB transcription factor, VvMYBC2L2, isolated from Vitis vinifera cultivar Yatomi Rose, may be involved in anthocyanin biosynthesis as a transcriptional repressor. VvMYBC2L2 was shown to be a nuclear protein. The gene was shown to be strongly expressed in root, flower and seed tissue, but weakly expressed during the fruit development in grapevine. Overexpressing the VvMYBC2L2 gene in tobacco resulted in a very marked decrease in petal anthocyanin concentration. Expression analysis of flavonoid biosynthesis structural genes revealed that chalcone synthase (CHS), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LAR) and UDP glucose flavonoid 3-O-glucosyl transferase (UFGT) were strongly down-regulated in the VvMYBC2L2-overexpressed tobacco. In addition, transcription of the regulatory genes AN1a and AN1b was completely suppressed in transgenic plants. These results suggested that VvMYBC2L2 plays a role as a negative regulator of anthocyanin biosynthesis.

Sign in / Sign up

Export Citation Format

Share Document