scholarly journals Changes in Soluble Sugars, Sugar Profile, Starch and Proline in Developing Coconut (Cocos nucifera L.) Inflorescences

CORD ◽  
2008 ◽  
Vol 24 (1) ◽  
pp. 7
Author(s):  
Bandupriya H.D.D.

Changes in soluble sugars, sugars profile, starch and proline levels in inflorescence rachillae from individual coconut palms were investigated during inflorescence development with the aim of determining a possible correlation between these characters and morphogenic potential of inflorescence tissues. Rachillae for analysis were collected from unopened inflorescences of -1 to -13 stages (considering the youngest open inflorescence as 0 stage) in decreasing order of maturity (–1 stage is the most mature stage whereas –13 is the most immature stage). Important differences among the maturity stages were observed for total sugars. In very tender inflorescences (-13 and -12), the total sugar content was very low whereas a gradual increase was observed from -11 to -7 stages, with  -7 stage having the highest level. The total sugar content in more mature inflorescences was relatively low, with the exception of -2 stage, which had a high total sugar content. In regard to sugar profiles, sucrose, fructose and glucose were the main soluble sugars present in coconut inflorescence and sucrose was the most abundant sugar in -5 to -9 maturity stages. Total soluble sugars and sucrose in maturity stages from -5 to -9 showed a very similar variation and significantly higher levels of sucrose were observed in -6 to -8 stages. The proline content in the mature stages, -1 to –3, was significantly lower than in the other stages with no significant variation in the stages –4 to –11. The pattern of variation in starch content was similar to that of proline which decreased with increasing maturity of inflorescence. In view of the results obtained, the higher accumulation of sucrose and total sugars in -6, -7 and -8 stages may have some significance in morphogenesis, especially as an energy source. The 10 cm length inflorescence that responds better for callusing falls within this range. Thus total sugar and sucrose content may be possible biochemical markers for assessing the morphogenic potential of inflorescence explants.

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 879A-879
Author(s):  
Teri Hale* ◽  
Richard Hassell ◽  
Tyron Phillips

Taste panel perception and preference of sweetness in three phenotypes (su, se and sh2) of sweet corn harvested at three maturities (early, mature and late) were compared to refractometer measurements and HPLC analysis of fructose, sucrose, and glucose. Panelist rating of sweetness and acceptability significantly correlated with HPLC analysis. These correlations were found for sucrose and total sugars present (for sweetness, r2 = 0.70 and 0.61; acceptability, r2 = 0.64 and 0.55). Sucrose significantly correlated with the total sugars present (r2 = 0.95). The panelists' perception of flavor also correlated significantly with the amount of sucrose present and total sugars (r2 = 0.66 and 0.59, respectively). Sucrose content was significantly different between se, sh2 and su, with sh2 having the highest level. Taste panels indicated this difference but showed not significant differnece between se and sh2 acceptablity. Su was only acceptable to panelists at early maturity. °Brix did not reflect the taste panels scores and HPLC measurements postively. Soluble solids and taste panel scores were negatively correlated in both the panel's perception of sweetness and acceptability (r2 = -0.66 and -0.66, respectively) which indicates that as panel scores decreased °Brix increased. Comparison of soluble solids to HPLC analysis, indicate that °Brix was negatively correlated to sucrose and total sugar content, and that as soluble solids increased, the sucrose or total sugar concentration remained constant or decreased. Soluble solids measurements have been positively correlated with sucrose levels in other crops; but this was not the case with sweet corn.


HortScience ◽  
2015 ◽  
Vol 50 (4) ◽  
pp. 577-581 ◽  
Author(s):  
Engin Ertan ◽  
Esra Erdal ◽  
Gülsüm Alkan ◽  
Burak E. Algül

This trial was initiated in the harvest season of 2010 to determine the effects of traditional and cold storage on the fruit quality properties of chestnuts during the harvest and postharvest periods. Physical and biochemical analyses were conducted on fruit samples collected about once every 2 weeks from the middle of September until the end of December. Specifically, the shell and kernel colors (hue, chroma), water activity (aw), and total sugar (%), total starch (%), total carbohydrate (%), and tannin (ppm) contents were determined. Under traditional and cold storage conditions, the total sugar content of the chestnuts increased whereas the total starch content decreased during the storage period. In addition, the maximum tannin content was measured in fruit that was cold stored for a period of 60 days.


Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2115
Author(s):  
Iwona Morkunas ◽  
Mehmet Zafer Doğu ◽  
Agnieszka Woźniak ◽  
Waldemar Bednarski ◽  
Jacek Kęsy ◽  
...  

The main aim of this study was to investigate metabolic responses of fruits, leaves, and shoots of pistachio trees (Pistacia vera L. cv. Kirmizi) during their development. Electron paramagnetic resonance spectroscopy revealed significant increase in generation of semiquinone radicals in fruits and leaves of pistachio, while the flower cluster thinning application was conducted in relation to the control; especially at the second term of the plant material collection. Moreover, flower abscission caused an increase in the level of phytohormones such as indole-3-acetic acid and abscisic acid in fruits at the first term of fruits sampling. In turn, high-performance liquid chromatography analysis revealed differences both in the profile as well as the contents of soluble sugars detected in pistachio organs. The highest total sugar content was found in fruits of pistachio where the flower cluster thinning application was made early. Total sugar levels were higher also in leaves and shoots of the above-mentioned pistachio plants in relation to the control until the third sampling time. The importance of high levels of fructose in the fruits following the flower abscission was observed. Additionally, analyses of mineral elements in organs showed that copper and phosphorus contents in fruits were higher after the flower abscission in relation to the control. To conclude, our findings signal on contribution of semiquinone radicals, paramagnetic manganese ions, phytohormones, nutrients, and sugars in pistachio organs development on the background of the flower cluster thinning which was applied before fructification.


2021 ◽  
Author(s):  
Rahul Govinda Kolwate ◽  
Ashish Ashok Uikey ◽  
S.R. Shegokar

Background: During present investigation burfi was prepared from different combinations of cow milk khoa and pumpkin pulp [viz., 100:00 (T1), 75:25 (T2), 70:30 (T3), 65:35 (T4) and 60:40 (T5)] and the samples were subjected to sensory evaluation, chemical analysis and cost was computed considering the ingredients and processing costs. The pumpkin pulp (as per treatments) and sugar (30% by weight of khoa) were mixed with khoa at different stage of khoa preparation. Then, the mixture was heated till burfi was obtained. Methods: For preparation of burfi fresh cow milk was obtained from Livestock Instructional Farm, Akola. The milk was standardized to 4.0 per cent fat and 9.0 per cent SNF for the preparation of burfi. The khoa blended with pumpkin (Cucurbita pepo) pulp at different ratios, the product without using pumpkin pulp served as control. The burfi was analyzed for chemical composition (viz., fat protein, total sugars, ash and total solids). The organoleptic attributes of burfi were analyzed in terms of its flavor, body and texture and color and appearance, wherein the total score was out of 100; the judging panel comprised on 5 members. Result: The total sensory scores of burfi obtained were 87.81, 90.46, 95.46, 92.99 and 84.30 for the treatment T1, T2, T3, T4 and T5 respectively. The burfi prepared from various combinations involving use of up to 70 per cent cow milk khoa in blend with pumpkin pulp was found acceptable. The fat content of burfi’s was 19.61, 15.40, 14.38, 13.40 and 12.37 per cent, protein content was 14.89, 12.64, 12.11, 11.61 and 11.04 per cent, total sugar content was 32.78, 36.73, 37.59, 38.45 and 39.31 per cent, ash content was 2.69, 2.55, 2.53, 2.49 and 2.45 per cent, total solids content was 69.94, 67.30, 66.60, 65.94 and 65.17 per cent for the treatment T1, T2, T3, T4 and T5 respectively. The fat, protein, ash and total solids of burfi tended to decrease while total sugar and moisture content tended to increase with an increase in the level of pumpkin pulp used as additive. The per kg cost of production of pumpkin based burfi decreased with increasing level of incorporation of pumpkin pulp in burfi formulation i.e. ₹ 240 (100:00 khoa), ₹ 204.51 (75:25, khoa: pulp), ₹ 199.11 (70:30 khoa: pulp), ₹ 194.33 (65:35 khoa: pulp), ₹ 189.25 (60:40, khoa : pulp) for the treatment T1, T2, T3, T4 and T5 respectively. The most acceptable burfi i.e. T3 was computed to be priced at ₹ 199.11 per kg.


2021 ◽  
Vol 8 (2) ◽  
pp. 283-288
Author(s):  
Tasneem Rangwala ◽  
Angurbala Bafna ◽  
Nagesh Vyas ◽  
Rohan Gupta

Fertilisers have become an important factor used by farmers to increase yield and improve product quality. Earth’s crust carries a large amount of elemental silicon. However, silicon is not considered an essential element for plant growth therefore is not included in fertilisers. Silicon has shown to enhance the growth and productivity of various crops. The present study aimed to explore the potential of soluble silica in improving the biochemical parameters of banana (Grand naine variety). The field experiment was conducted at Ropni Vasaad village, Burhanpur District of Madhya Pradesh from August 2017 to September 2018. Silica was supplied as potassium silicate in the liquid form under the trade name AgriboosterTM. Doses were administered at the interval of one month starting from planting the tissue culture explants till harvesting the final crop. Eight treatments were designed which included three different concentration of soluble silica applied alone and with combination with compound fertilisers. Control was without any treatment. A significant increase in fresh and dry weight was observed with all the treatments. All the combinations of soluble silica resulted in significant increase in starch and total sugar content. The protein content showed significant increase with treatments consisting of soluble silica and compound fertiliser. Cellulase and amylase activity declined on treatment with soluble silica. The present study reveals that if soluble silica is either applied alone or with compound fertiliser, it can enhance the biochemical parameters and can indirectly delay ripening of banana by altering activity of cellulase and amylase.


2005 ◽  
Vol 15 (3) ◽  
pp. 668-672 ◽  
Author(s):  
Teri A. Hale ◽  
Richard L. Hassell ◽  
Tyron Phillips

The refractometer has been proposed as a rapid, inexpensive technique for determining sugar levels in fresh sweet corn (Zea mays). High performance liquid chromatography (HPLC) analysis of sugars in three phenotypes (su, se, and sh2) of sweet corn harvested at three maturities indicated that sucrose content was highly correlated with the total sugars (R = 0.95). Sucrose and total sugar concentration were significantly different among all phenotypes. Soluble solids concentration (SSC) was high in su and se compared to the lower SSC of sh2. Early, mature, and late harvested samples differed in sucrose and total sugar content. Sugar concentration varied within phenotypes at each maturity level. Sh2 indicated no difference in sucrose and total sugars at early and mature harvests, but increased at late harvest. In contrast, sucrose and total sugar content decreased between early and mature harvests, then increased to highest levels at late harvest in se and su phenotypes. Overall, phenotype SSC increased significantly from early to late harvests, probably due to increased water-soluble polysaccharides in the su and se cultivars. Unlike other crops, a negative relationship was found in sweet corn between SSC and sucrose or total sugars, with an overall correlation of –0.51. This relationship was most affected by maturity, especially mature and late harvested sweet corn. Among phenotypes, sucrose, total sugar, and SSC were poorly correlated. Our results indicate that a refractometer should not be used to estimate total sugars or sucrose of sweet corn.


1970 ◽  
Vol 46 (4) ◽  
pp. 443-446 ◽  
Author(s):  
MM Rahman ◽  
MM Rahman ◽  
N Absar ◽  
MA Ahsan

Two good qualities of mango cultivars namely Fazli and Khirshapat, harvested at the full mature stage i.e. begining of ripening, have been stored at -5°C, 4°C and 25±2°C for 10 days and the changes in the content of amylase, invertase, β -galactosidase, starch, total sugar, reducing sugar and non-reducing sugar of the pulp were analyzed after 2-days regular interval of time. The activities of all the enzymes examined in the study were found to be higher in mango-pulp at 25°C as compared to those in 4°C or -5°C. The activities of amylase and invertase decreased after 8-days of storage while that of β -galactosidase increased with the increasing days of storage mango pulp. Total sugar and non-reducing sugar content of mango were increased gradually while starch content was decreased sharply with the increasing days of storage of mature mango. Changes in carbohydrate during storage of mango was found to be associated with the content of amylase, invertase and β -galactosidase. At -5°C the enzymes as well as carbohydrate content of storage mango pulp changed slightly upto 4- days and thereafter the enzyme became fully dormant. Key Words: Ripe mango; Carbohydrate; Amylase; Invertase; β -galactosidase; Temperatures DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9588 BJSIR 2011; 46(4): 443-446


2007 ◽  
Vol 87 (2) ◽  
pp. 327-332 ◽  
Author(s):  
Metka Hudina ◽  
Mateja Colarič ◽  
Franci Štampar

Primary metabolites (sugars and organic acids) in leaves and fruits of the pear cultivars Conference, Concorde and Williams were investigated during the growing season in Slovenia. Leaves were sampled 15 times from Apr. 25 to Oct. 22. Fruits were sampled seven times from Jun. 14 to Aug. 23. The contents of sugars (glucose, fructose, sucrose, and sorbitol) and organic acids (malic, citric, shikimic, and fumaric) in leaves and fruit were determined by high performance liquid chromatography (HPLC). Similar dynamic patterns were found in the glucose, fructose, sucrose and sorbitol in leaves and fruits of the genetically related cultivars Conference and Concorde. Leaf sugar was low at the beginning of the growing season when the leaves were not completely developed. Generally when sucrose increased in leaves it decreased in fruits. At the end of June the total sugar content in leaves reached its peak then rapidly decreased. At the same time, total sugar in fruits increased. From the beginning of August, total sugars in fruits increased regardless of the sugar content in leaves and likely due to decomposition of starch. After harvest, the contents of individual sugars (glucose, fructose, sucrose, and sorbitol) in the leaves decreased until the beginning of October when, just prior to leaf drop, they increased in all cultivars. Key words: Pyrus communis, sugars, organic acids


1989 ◽  
Vol 69 (3) ◽  
pp. 967-977 ◽  
Author(s):  
F. J. BIGRAS ◽  
J. A. RIOUX ◽  
H. P. THERRIEN ◽  
R. PAQUIN

Three-year-old plants of Juniperus chinensis ’Pfitzerana Aurea’ grown in containers were fertilized until August, September or October. Frost tolerance, water, mineral, total sugar, and starch content of shoots and roots were followed during the fall. Fall fertilization increased N and K content in the shoots and roots and accelerated shoot spring growth. Contents of P, Ca, Mg, Fe and Mn in the shoots and roots were not modified by the late fertilization regimes in the fall. Hardening of mature root and shoot was not affected by the prolongation of fertilization. Young roots did not harden. Water content of shoots decreased during hardening. The variability of root water content was too important to allow a detection of any reduction. At the end of fall, starch was almost completely hydrolyzed in shoots while it remained in appreciable quantity in roots. Total sugars increased more in shoots than in roots during the fall. The prolongation of fertilization had no influence on the total sugar or starch accumulation in shoots. Prolongation of the fertilization until October increased the starch and sugar content in roots.Key words: Juniperus chinensis ’Pfitzerana Aurea’, cold hardiness, growth, fertilization, water content, sugars, starch


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