scholarly journals PELATIHAN PROSEDUR LABORATORIUM ANALISIS DNA

2018 ◽  
Vol 2 (2) ◽  
pp. 44-48
Author(s):  
Dewi Indriyani Roslim ◽  
Herman Herman ◽  
Roza Elvyra ◽  
Nery Sofiyanti ◽  
Ennie Chahyadi
Keyword(s):  
Polymerase Chain Reaction ◽  
Natural Disasters ◽  
Dna Analysis ◽  
Dna Isolation ◽  
Research Centers ◽  
Chain Reaction ◽  
Paternity Test ◽  
Analysis Laboratory ◽  
Laboratory Procedures ◽  
Polymerase Chain

Currently, analysis based on DNA is one of the techniques for solving the problems such as paternity test, determining the identity of accident victims, natural disasters victims, and authentication of foods ingredients. But not everyone knows about the stages of DNA analysis laboratory procedures. The purpose of this workshop is to provide knowledgments about DNA analysis laboratory procedures. The training was attended by 30 participants from various universities and research centers in Riau Province. Workshop materials include DNA isolation of animal, plant and bacterial, electrophoresis, polymerase chain reaction (PCR), and visualization of DNA bands. Evaluation uses a questionnaire distributed to participants. Questionnaire analysis showed that workshop was very useful especially for beginners who want to be involved in DNA analysis activities in the laboratory.

Evaluation of polymerase chain reaction and DNA isolation protocols for detection of genetically modified soybean

2007 ◽  
Vol 42 (10) ◽  
pp. 1249-1255 ◽  
Author(s):  
Cibele dos Santos Ferrari ◽  
Luciana Lehmkuhl Valente ◽  
Fábio Cristiano Angonesi Brod ◽  
Caroline Tagliari ◽  
Ernani Sebastião Sant'Anna ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Isolation ◽  
Genetically Modified ◽  
Chain Reaction ◽  
Genetically Modified Soybean ◽  
Polymerase Chain

DNA Isolation from Recalcitrant Materials Such as Tree Roots, Bark, and Forest Soil for the Detection of Fungal Pathogens by Polymerase Chain Reaction

1998 ◽  
Vol 262 (1) ◽  
pp. 79-82 ◽  
Author(s):  
Günther Bahnweg ◽  
Steffen Schulze ◽  
Evelyn M. Möller ◽  
Hilkea Rosenbrock ◽  
Christian Langebartels ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Forest Soil ◽  
Fungal Pathogens ◽  
Dna Isolation ◽  
Chain Reaction ◽  
Tree Roots ◽  
Polymerase Chain

scholarly journals Method Comparison of DNA Isolation and Quantification for Fish and Seafood Authenticity Determination

2018 ◽  
Vol 13 (3) ◽  
pp. 115
Author(s):  
Dwiyitno Dwiyitno ◽  
Stefan Hoffman ◽  
Koen Parmentier ◽  
Chris Van Keer
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Dna Isolation ◽  
Blue Mussel ◽  
Pcr Amplification ◽  
Amplification Efficiency ◽  
Chain Reaction ◽  
Seafood Products ◽  
Polymerase Chain ◽  
Commercial Kit

Fish and seafood products has been commonly targeted for fraudulent activities. For that reason, authentication of fish and seafood products is important to protect consumers from fraudulent and adulteration practices, as well as to implement traceability regulation. From the viewpoint of food safety, authenticity is beneficial to protect public from serious food poisoning incidents, such as due to ingestion of toxic species. Since DNA based identification depends on the nucleic acid polymerase chain reaction (PCR), the quantity and quality/purity of DNA will contribute significantly to the species authentication. In the present study, different DNA extraction and purification methods (3 classical methods and one commercial kit) were compared to produce the better isolated DNA for PCR amplification. Additionally, different methods for the estimation of DNA concentration and purity which is essential for PCR amplification efficiency were also evaluated. The result showed that classical DNA extraction methods (based on TNES-Urea) yielded a higher amount of DNA (11.30-323.60 ng/g tissue) in comparison to commercial kit/Wizard Promega (5.70-83.45 ng/g tissue). Based on the purity of DNA extract (A260/280), classical DNA extraction method produced relatively similar on DNA quality to the commercial kit (1.79-2.12). Interestingly, all classical methods produced DNA with A260/280 ratio of more than 2.00 on the blue mussel, in contrast with commercial kit. The commercial kit also produced better quality of DNA compared to the classical methods, showing the higher efficiency in PCR amplification. NanoDrop is promising as cheap, robust and safe UV-spectrophotometer method for DNA quantification, as well as the purity evaluation.Keywords: seafood authenticity, DNA isolation, polymerase chain reaction, NanoDrop, Picogreen

scholarly journals 484. DNA analysis with polymerase chain reaction (PCR) of bacterial infections

1992 ◽  
Vol 48 (8) ◽  
pp. 1567
Author(s):  
Takashi Arai ◽  
Hideaki Yamamoto ◽  
Satoshi Kobayashi ◽  
Akira Yamaguchi ◽  
Katsumi Ikei
Keyword(s):  
Polymerase Chain Reaction ◽  
Bacterial Infections ◽  
Dna Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Циклостойкие миниатюрные термоэлектрические модули

2019 ◽  
Vol 53 (5) ◽  
pp. 604
Author(s):  
М.П. Волков ◽  
И.А. Драбкин ◽  
Л.Б. Ершова ◽  
А.А. Назаренко
Keyword(s):  
Polymerase Chain Reaction ◽  
Test Data ◽  
Medical Equipment ◽  
Dna Analysis ◽  
Heat Fluxes ◽  
Chain Reaction ◽  
Periodic Heating ◽  
Thermoelectric Modules ◽  
Heating And Cooling ◽  
Polymerase Chain

AbstractIn the paper the test data on new cycle-resistant thermoelectric modules are presented and discussed. These modules can be applied in medical equipment for polymerase chain reaction (PCR) to carry out DNA analysis with the help of rapid periodic heating and cooling of biological probes. However, high density of heat fluxes and, as a result, significant mechanical stresses in miniature thermoelectric modules involve special requirements to their reliability. The company RMT Ltd. has developed a technology for the production of highly reliable miniature thermoelectric modules that allowed them to withstand more than 500 thousand heating-cooling cycles (from 20 to 100°C) with a rate of 20°C/s and more.

A novel buffer system, AnyDirect, can improve polymerase chain reaction from whole blood without DNA isolation

2007 ◽  
Vol 380 (1-2) ◽  
pp. 112-117 ◽  
Author(s):  
Young Geun Yang ◽  
Jong Yeol Kim ◽  
Young-Han Song ◽  
Doo-Sik Kim
Keyword(s):  
Polymerase Chain Reaction ◽  
Whole Blood ◽  
Dna Isolation ◽  
Buffer System ◽  
Chain Reaction ◽  
Polymerase Chain

Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Kemal Kazan ◽  
John M. Manners ◽  
Don F. Cameron
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Interspecific Cross ◽  
Parental Origin ◽  
Chain Reaction ◽  
Stylosanthes Hamata ◽  
Polymerase Chain

The inheritance of random amplified polymorphic DNA (RAPD) markers generated via the polymerase chain reaction amplification of genomic DNA sequences in an F2 family of an interspecific cross between Stylosanthes hamata and S. scabra was investigated. An initial comparison between the parental species, S. hamata cv. Verano and S. scabra cv. Fitzroy, demonstrated that 34% of detected RAPD bands were polymorphic. Of 90 primers tested, 35 showed relatively simple and reliably scorable polymorphisms and were used for segregation analysis. Sixty F2 individuals were scored for the segregation of 73 RAPD markers and 55 of these markers fit a 3:1 ratio. Segregation of eight other RAPD markers deviated significantly from a 3:1 ratio. There was no bias in the inheritance of RAPD markers regarding parental origin of the segregating RAPD markers. Linkage analysis revealed 10 linkage groups containing a total of 44 RAPD loci. Another 10 RAPD markers (7 of maternal origin) that were polymorphic between the parents did not segregate in the F2 population. One of the maternally inherited RAPD bands hybridized to chloroplast DNA. Analysis of RAPD loci by DNA hybridization indicated that mainly repeated sequences were amplified. These data indicate that RAPDs are useful genetic markers in Stylosanthes spp. and they may be suitable for genetic mapping.Key words: genetic mapping, molecular markers, polymerase chain reaction, Stylosanthes hamata, Stylosanthes scabra.

Random amplified polymorphic DNA analysis in Hordeum species

Genome ◽  
1993 ◽  
Vol 36 (6) ◽  
pp. 1029-1031 ◽  
Author(s):  
Juan Manuel González ◽  
Esther Ferrer
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Fragment Pattern ◽  
Polymerase Chain ◽  
Hordeum Species

Random amplified polymorphic DNA analysis was performed by applying a set of 13 arbitrary 10-mer primers to 19 Hordeum species and subspecies. High levels of variation in fragment pattern were observed both within and among species with most of the primers used. Genetic similarities between accessions and species were calculated from the fragment patterns. The resulting phenograms confirmed previous relationships among the Hordeum species.Key words: random amplified polymorphic DNA, polymerase chain reaction, polymorphism, Hordeum.

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