Microbial spectrum and antibiotic susceptibility of uropathogens causing Urinary tract infection in pregnant women

2011 ◽  
Vol 1 (2) ◽  
pp. 77-80
Author(s):  
Pallavi Jayavanth ◽  
Junainah Abd Hamid ◽  
Mathan Mohan ◽  
Mohamed Rafi ◽  
Rajasegar Anamalley ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Antibiotic Susceptibility ◽  
Antibiotic Resistant ◽  
Isolation And Characterization ◽  
Antimicrobial Surveillance ◽  
Antibiotic Susceptibility Test ◽  
Urine Specimens

Urinary tract infection (UTI) is an intermittent cause of sickness in the pregnant women. A study was conducted to identify the microbial gamut and antibiotic vulnerability patterns of bacteria isolated from pregnant women with suspected urinary tract infection. In total, 75 urine specimens were screened for bacteriuria over a period of four months. Aseptically collected urine specimens were subjected to standard isolation and characterization procedures. Antibiotic susceptibility test was performed according to CLSI (2007). Five types of bacteria were isolated namely Escherichia coli 32(55%), Staphylococcus aureus 12 (21%), Enterococcus Sp 7 (12%), Klebseilla pneumoniae 4 (7%) and Pseudomonas aeruginosa 3 (5%). All bacterial isolates exhibited efficacy to all the antibiotics namely fosfomycin (50mg), ciprofloxacin (10mg), trimethoprim & sulfamethoxazole (25 mg), ampicillin (10 mg), nitrofurantoin (300 mg) and cefazolin (30mg) except amoxycillin. Ampicillin and ciprofloxacin exhibited illustrious efficacy at low concentration. Regular antimicrobial surveillance and reinforcement of appropriate antibiotic regimen in pregnant women with UTI will restrain the re‐emergence of antibiotic resistant uropathogens.

scholarly journals Prevalence of Asymptomatic Bacteriuria during Pregnancy at a Tertiary Care Hospital of Province No. 2, Nepal

2019 ◽  
Vol 6 ◽  
pp. 32-38
Author(s):  
Khushbu Yadav ◽  
Satyam Prakash
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Antibiotic Susceptibility ◽  
Tertiary Care ◽  
Asymptomatic Bacteriuria ◽  
Diffusion Method ◽  
Care Hospital ◽  
Antibiotic Susceptibility Test

Objectives: The objective of this study was to determine the prevalence of asymptomatic bacteriuria in pregnant women, identify the causative agent responsible for urinary tract infection (UTI) and its antibiotic susceptibility. Methods: The mid-stream urine sample was streaked on the MacConkey agar (MA) and Blood agar (BA) medium by the semi-quantitative culture technique. Identification of significant isolates was done by standard microbiological techniques. Antibiotic susceptibility test of the isolated organisms was done by modified Kirby Bauer disc diffusion method. Results: The prevalence rate of asymptomatic urinary tract infection (AUTI) among pregnant women was found to be 42%. The highest number of UTI cases found during pregnancy was in between age 21-25 years (52.22%), in second gravida (51.59%), during 3rd trimester of pregnancy (49.68%) and in winter with 52.22%. E. coli was principal organism to cause AUTI (35.48%) during pregnancy. Amikacin, imipenem and nalidixic acid were effective towards Gram negative bacilli whereas vancomycin, tetracycline and amoxyclav were effective towards Gram positive cocci. Conclusion: All pregnant women visited for antenatal checkups should be advised for the culture and sensitivity test of their urine specimens which will reduce the maternal and child health complications. Different screening test and awareness programme should be conducted at regular interval of time for prevention of AUTI during pregnancy.

scholarly journals 1472. Non-Carbapenems for Treating Community-Associated Urinary Tract Infection Caused by Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Children

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S537-S537
Author(s):  
Hyun Joo Jung ◽  
Shin Young Lee
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Susceptibility ◽  
University Hospital ◽  
E Coli ◽  
Extended Spectrum ◽  
Antibiotic Susceptibility Test ◽  
Higher Sensitivity

Abstract Background Childhood urinary tract infection (UTI) may cause increased major morbidity and long-term clinical consequences. Extended-spectrum β-lactamase (ESBL) is produced by the members of the Enterobacteriaceae family, which are the primary infectious agents that cause UTI in children. Isolation of ESBL-producing Enterobacteriaceae (ESBL-E) typically occurred in healthcare facilities; however, the incidence of community-associated (CA) UTIs due to ESBL-E has increased worldwide. It has led to an increase in the use of carbapenems. In this study, we determine the characteristics of community-onset UTIs caused by ESBL-E in children to suggest non-carbapenem options for the treatment of childhood UTIs due to ESBL-E in order to preserve carbapenems. Methods A total of 2,157 isolates of ESBL-E were collected from children below 18 years old who were clinically certified UTI or urosepsis between January 2008 and August 2018 at tertiary university hospital in Korea. Their electronic medical records were retrospectively reviewed. Long-term healthcare facility stay within the preceding month and isolates recovered more than 72 hours after hospitalization were the criteria of healthcare-associated (HA) infection. Results The most common isolates were E. coli 1815 (84.2%) followed by K. pneumoniae 342 (15.8%). CA infection was detected in 1,513 of the 2157 ESBL-E (70.1%). The prevalence of CA ESBL-E infection increased significantly from 68 cases in 2008 to 325 cased in 2017. Antibiotic susceptibility test showed highest sensitivity to ertapenem, meropenem, and amikacin (>90%) followed by cefoxitin (82%), and piperacillin–tazobactam (TZP) (80.5%). CA E. coli showed higher sensitivity to amikacin and TZP compared with HA E coli. CA K. pneumoniae showed much higher sensitivity to TZP compared with HA K. pneumoniae. Of total ESBL-E, the antimicrobial resistance rate to aminoglycoside such as amikacin and gentamicin showed full sensitivity during the study period; furthermore, a rate of resistance to TZP has been decreasing over the years. Conclusion Identifying antibiotic susceptibility patterns of ESBL-E is a useful guide for treatment strategy of UTI. This study showed that there are non-carbapenem options for the treatment of CA ESBL UTI in children. Disclosures All authors: No reported disclosures.

Rapid genotypic antibiotic susceptibility test using CRISPR-Cas12a for urinary tract infection

The Analyst ◽  
2020 ◽  
Vol 145 (15) ◽  
pp. 5226-5231
Author(s):  
Juhong Chen ◽  
Fuguo Jiang ◽  
Chao-Wei Huang ◽  
Liwei Lin
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Susceptibility ◽  
Good Accuracy ◽  
Susceptibility Test ◽  
New Method ◽  
Antibiotic Susceptibility Test

A new method based on the technique of CRISPR-Cas12a is developed to accomplish a bacterial antibiotic susceptibility test (AST) within one hour with good accuracy.

scholarly journals Incidence of Nosocomial Urinary Tract Infections and Antibiotic Susceptibility Pattern of Uropathogenic Bacterial Isolates of Patients from Selected Hospitals in Nsukka, Nigeria

2021 ◽  
pp. 7-16
Author(s):  
C. O. Aleke ◽  
K. O. Ugwu ◽  
O. N. Akoma ◽  
I. M. Ezeonu
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urinary Tract Infections ◽  
Antibiotic Susceptibility ◽  
Bacterial Isolates ◽  
Susceptibility Pattern ◽  
Antibiotic Susceptibility Pattern ◽  
Tract Infections ◽  
Urine Specimens

Urinary Tract Infection (UTI) is one of the significant healthcare and hygienic-based diseases ravaging millions of people, especially in third world countries with poor hospital settings. This cross-sectional and laboratory investigation was done to determine the incidence of nosocomial urinary tract infections (NUTIs) and antibiotic susceptibility pattern of the uropathogenic bacterial isolates from hospital patients in Nsukka, Southeastern Nigeria. Urine specimens of fifty patients were collected and analyzed between May and June 2018. Kirby-Bauer disk diffusion method was used for detection of antibiotic susceptibility pattern for all isolates. Bacteriuria was observed in urine specimens of 31 (62%) out of the 50 participants, with the specimens of 13 yielding significant growth at 42% rate of nosocomial urinary tract infections (NUTIs). The most predominant bacterial isolate of urinary tract infection was Staphylococcus spp. (31%); followed by Escherichia, Klebsiella and Proteus with (23%) each. Isolates were mostly susceptible to Ciprofloxacin (87.1%), while other isolates were mainly resistant to Zinnacef (937%). Pertinent recommendations were made with respect to treatment, multidrug resistance mitigation and aseptic and optimal health services delivery practices.

scholarly journals Isolation and Characterization of Antibiotic-Resistant Bacteria from Urinary Tract Infection on Diabetic Mellitus Patients

2020 ◽  
Vol 12 (3) ◽  
pp. 391-396
Author(s):  
M. Kumar ◽  
L. Prince
Keyword(s):  
Diabetes Mellitus ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Resistant Bacteria ◽  
Klebsiella Aerogenes ◽  
Diabetic Mellitus ◽  
Antibiotic Resistant ◽  
Isolation And Characterization

Diabetes mellitus is a chronic disease-which occurs when the pancreas does not produce enough insulin, or when the body cannot effectively use the insulin it produces. This leads to an increased concentration of glucose in the blood (hyperglycemia). Diabetes mellitus is one of the most challenging health problems in India. The present study was investigated for isolation and characterization of antibiotic-resistant bacteria from urinary tract infection on diabetic mellitus patients. The 118 diabetic urine samples were collected and UTI bacteria using HiChrome UTI Agar was isolated. The diabetic UTI isolates were confirmed as Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus saprophyticus, Proteus mirabilis and Klebsiella aerogenes based on morphological and biochemical characteristics. Our study identified that almost all the bacteria were highly sensitive to Cefoperazone/Sulbactam (75/30 mcg), Gentamicin (10 mcg), Meropenem (30 mcg), Piperacillin/Tazobactam (100/10 mcg) and Nitrofurantoin (100 mcg) antibiotics. Interestingly Klebsiella aerogenes alone was found to be more resistant to the entire antibiotics used in this study. The antibiotic resistant Klebsiella aerogenes is one of the biggest treats to human health, antibiotic resistance occurs naturally, but misuse of antibiotics in humans is accelerating the process.

scholarly journals POLA BAKTERI DAN TES KEPEKAAN ANTIBIOTIKA WANITA HAMIL DENGAN BAKTERIURIA ASIMTOMATIS

2018 ◽  
Vol 12 (3) ◽  
pp. 103
Author(s):  
L. P. Kalalo ◽  
Aryati . ◽  
B. Subagjo
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Clavulanic Acid ◽  
Low Birthweight ◽  
Asymptomatic Bacteriuria ◽  
Antibiotic Sensitivity ◽  
Antibiotic Susceptibility Test ◽  
Amoxicillin Clavulanic Acid

Asymptomatic bacteriuria is defined as bacteriuria consisting of one type of bacteria ³ 105 CFU/mL in clean-voided midstreamculture of a person without any symptoms of urinary tract infection. Untreated asymptomatic bacteriuria is dangerous for pregnancybecause complications such as pyelonephritis, maternal sepsis, premature birth, and premature rupture of the membrane, low birthweight baby, hypertension and or preeclampsia, anaemia in pregnancy, amnionitis and post delivery endometriosis can arise. Someasymptomatic cases could threaten the pregnant woman and her foetus without prior pyelonephritis symptoms. The purpose of this studywas to determine the pattern of bacteria in asymptomatic bacteriuria pregnant women and their antibiotic sensitivity. This study hasbeen carried out between October 2003 and April 2004 on 376 pregnant women without symptoms of urinary tract infection. Screeningwas performed using urine culture, followed by antibiotic susceptibility test. The study showed that the prevalence of asymptomaticbacteriuria among pregnant women in RSU Dr. Soetomo was 9.85% (37 out of 376 pregnant women without urinary tract infection).Thirteen bacterial types were found in those 37 positive samples. The most common bacteria found was Escherichia coli (37.83%). Thisbacterium was sensitive to ceftriaxone (85.71%), nitrofurantoin (64.28%) and amoxicillin-clavulanic acid (57.14%). Based on the datagained in this study, it is concluded that the prevalence of pregnancy cases with asymptomatic bacteriuria in the Dr. Soetomo Hospitalis significantly high (9.85%). The most recommended drugs are ceftriaxone, amoxicillin-clavulanic acid and nitrofurantoin.

Risk Factors in Diabetes and Pregnant Women with Urinary Tract Infections Compared to Younger Aged Female.

2018 ◽  
Vol 1 (3) ◽  
pp. 26-38
Author(s):  
Abdulghani Mohamed Alsamarai ◽  
Shler Ali Khorshed
Keyword(s):  
Risk Factors ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Female Student ◽  
Mean Value ◽  
Infected Female ◽  
Significant Difference ◽  
Tract Infections

Background: Urinary tract infection is common with health impact in women and characterised by failure to treatment and recurrent episodes. Aim: This study was conducted to determine the risk factors for the development of urinary tract infection in diabetic and pregnant women in comparison to student female. Materials and methods: A prospective cross-sectional study conducted during the period from 1st of June 2015 to the end of January 2016. The population included in the study are 563 women, of them 425 were outpatients, and 138 were inpatients. Their age range between 18 and 80 years, with a mean age of 33.59±15.29 years. Urine samples collected and cultured on blood agar and MacConkey agar by spread plate technique. Bacterial colonies with different morphology were selected, purified and identified according to their biochemical characteristics using conventional standard methods. Results: In diabetic women, there were no significant difference in mean age and BMI values between culture positive and culture negative groups. However, pus cell mean scale was significantly higher [P=0.000] in women with urinary tract infection [1.76±1.25] than in those with negative culture [0.69±1.00]. In pregnant women, BMI mean value was significantly [P=0.013] lower in pregnant women with UTI [26.14] as compared to those without infection [26.99]. Pus cell scale mean value was significantly [P=0.000] higher in pregnant women with UTI [1.55] than women with negative UTI [0.85]. While there was no significant difference in mean age between UTI positive and negative pregnant women. In female student, there was a significant difference between UTI infected and non-infected in mean age [P=0.041] and pus cell scale [P=0.000]. However, BMI was not significantly different between infected and non-infected female student. Other risk factors association are variables in the 3 groups when analysed using X2, while AUC and OR show different trends of association between risk factors and UTI. Conclusion: BMI, pus cell scale, child number, delivery method, operation history and hospital setting were significantly associated with culture positivity in the 3 studied groups as determined by AUC. While OR confirmed association with pus sale scale in the 3 groups.

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