Biological Activity of Ethanol Extracts from Amelanchier asiatica Fruits

The six Dioscorea species, D. brevipetiolata, D. bulbifera, D. depauperata (Dd), D. glabra (Dg), D. pyrifolia and D. hamiltonii were analyzed for phytochemicals, toxicity in PBMCs, and biological activity in two cancer cell lines by MTT and comet assays, and pesticide efficiency. Via GC-MS, lidocaine was found to be the predominant compound in two of the studied species. To confirm the systematics, lidocaine was also found in lower amounts in 11 species. The MTT assay showed no toxicity in all six of the studied species. The comet assay showed the key result that the ethanol extracts of Dd and Dg violently broke DNA into pieces. Biological activity of these two species’ extracts showed toxicity on HepG2 and no effects on HCT-116. The water extracts of Dd and Dg, applied to Brassica chinensis showed high efficiency as a bioprotectant. In summary, lidocaine seems to be the predominant identifying compound of the genus Dioscorea in Thailand, which is useful in systematics. At least the two species, Dd and Dg, may be used for human hepatocyte cancer treatment and as an alternative pesticide for economically important vegetables. Dioscorea species containing lidocaine or extracted lidocaine have promise for natural product creation.

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Biological Activity Study on Anti-oxidant, Whitening, and Anti-inflammatory Effects of Astragalus membranaceus Ethanol Extracts and Bioconversion Extracts

Asian Journal of Beauty and Cosmetology ◽

10.20402/ajbc.2017.0167 ◽

2017 ◽

Vol 15 (4) ◽

pp. 489-499 ◽

Cited By ~ 2

Author(s):

Hye-Kyung Bae ◽

Seon-Hee You

Keyword(s):

Biological Activity ◽

Astragalus Membranaceus ◽

Anti Oxidant ◽

Anti Inflammatory ◽

Ethanol Extracts

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Diversity of the Chemical Profile and Biological Activity of Capsicum annuum L. Extracts in Relation to Their Lipophilicity

Molecules ◽

10.3390/molecules26175215 ◽

2021 ◽

Vol 26 (17) ◽

pp. 5215

Author(s):

Barbara Chilczuk ◽

Beata Marciniak ◽

Renata Kontek ◽

Małgorzata Materska

Keyword(s):

Biological Activity ◽

Chemical Composition ◽

Antiradical Activity ◽

Total Content ◽

Sweet Pepper ◽

Hot Pepper ◽

Dpph Radical ◽

Chemical Profile ◽

Ethanol Extracts ◽

Unique Chemical

Ethanol extracts of two types of pepper (sweet and hot) were separated into fractions with increasing lipophilicity. After drying the extracts and fractions, their chemical composition, anti-radical activity in the DPPH radical system, and cytotoxic activity against PC-3 and HTC-116 cells were determined. A detailed qualitative analysis of the fractions was performed with the LC-QTOF-MS method. It was found that the chemical composition of pepper fractions did not always reflect their biological activity. The highest antiradical activity was detected in the fraction eluted with 40% methanol from sweet pepper. The highest total content of phenolic compounds was found in an analogous fraction from hot pepper, and this fraction showed the strongest cytotoxic effect on the PC-3 tumour line. The LC-MS analysis identified 53 compounds, six of which were present only in sweet pepper and four only in hot pepper. The unique chemical composition of the extracts was found to modulate their biological activity, which can only be verified experimentally.

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A comparative study of supercritical fluid and ethanol extracts of cannabis inflorescences: Chemical profile and biological activity

The Journal of Supercritical Fluids ◽

10.1016/j.supflu.2021.105385 ◽

2022 ◽

Vol 179 ◽

pp. 105385

Author(s):

Santiago Fernández ◽

Tatiana Carreras ◽

Rossina Castro ◽

Karen Perelmuter ◽

Victoria Giorgi ◽

...

Keyword(s):

Biological Activity ◽

Comparative Study ◽

Supercritical Fluid ◽

Chemical Profile ◽

Ethanol Extracts

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Ruta graveolens Extracts and Metabolites against Spodoptera frugiperda

Natural Product Communications ◽

10.1177/1934578x1501001137 ◽

2015 ◽

Vol 10 (11) ◽

pp. 1934578X1501001

Author(s):

Benjamín A. Ayil-Gutiérrez ◽

Jesús M. Villegas-Mendoza ◽

Zuridai Santes-Hernández ◽

Alma D. Paz-González ◽

Maribel Mireles-Martínez ◽

...

Keyword(s):

Biological Activity ◽

Ethyl Acetate ◽

High Mortality ◽

Spodoptera Frugiperda ◽

Leaf Tissue ◽

Ruta Graveolens ◽

Tissue Extracts ◽

Metabolite Quantification ◽

Ethanol Extracts

The biological activity of Ruta graveolens leaf tissue extracts obtained with different solvents (ethyl acetate, ethanol, and water) and metabolites (psoralen, 2-undecanone and rutin) against Spodoptera frugiperda was evaluated. Metabolites levels in extracts were quantified by HPLC and GC. Ethyl acetate and ethanol extracts showed 94% and 78% mortality, respectively. Additionally, psoralen metabolite showed a high mortality as cypermethrin. Metabolite quantification in extracts shows the presence of 2-undecanone (87.9 μmoles mg−1 DW), psoralen (3.6 μmoles mg−1 DW) and rutin (0.001 μmoles mg−1 DW). We suggest that these concentrations of 2-undecanone and psoralen in R. graveolens leaf tissue extracts could be responsible for S. frugiperda mortality.

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Assessment of the Biological Activity and Phenolic Composition of Ethanol Extracts of Pomegranate (Punica granatum L.) Peels

Molecules ◽

10.3390/molecules25245916 ◽

2020 ◽

Vol 25 (24) ◽

pp. 5916

Author(s):

Željka Peršurić ◽

Lara Saftić Martinović ◽

Mladenka Malenica ◽

Ivana Gobin ◽

Sandra Pedisić ◽

...

Keyword(s):

Biological Activity ◽

Antiproliferative Activity ◽

Biological Activities ◽

Punica Granatum ◽

Mass Spectrometry Analysis ◽

Total Phenolic ◽

Pomegranate Peel ◽

Spectrometry Analysis ◽

Ethanol Extracts ◽

Punica Granatum L

Pomegranate (Punica granatum L.) is a rich source of constituents with confirmed strong biological activities. However, pomegranate peel, which encompasses approximately 30–40% of its weight, is treated as a biological waste. The aim of this paper was to evaluate the potential of pomegranate peel extracts and to propose its functional properties that can be used for development of functional products. Eight ethanol extracts of pomegranate peels (PPEs) were characterized by use of direct infusion quadrupole-time of flight (Q-TOF), and afterwards tested on their antioxidant, antibacterial and antiproliferative activities. Mass spectrometry analysis revealed that the most prevalent compounds in pomegranate peels were punicalagin, granatin and their derivatives. Analysed extracts had high total phenolic contents that ranged from 5766.44 to 10599.43 mg GAE/100 g, and strong antioxidant activity (7551.31–7875.42 and 100.25–176.60 μmol TE/100 g for DPPH and FRAP assays, respectively). The results of biological activity assays showed that all PPEs possessed antibacterial activity, and that S. aureus was the most sensitive specie with minimum inhibitory concentration and minimum bactericidal concentrations ranging from 0.8 to 6.4 mg/mL. Additionally, the analysis of antiproliferative activity revealed high potency of PPEs, as the IC50 values ranged from 0.132 mg/mL to 0.396 mg/mL. Multivariate analysis pointed out the most discriminative metabolites for antioxidant or antiproliferative activity. Overall, the pomegranate peel confirmed to be a highly valuable source of bioactive compounds that could be used to improve the food functional characteristics.

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