scholarly journals Assessment of the Biological Activity and Phenolic Composition of Ethanol Extracts of Pomegranate (Punica granatum L.) Peels

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5916
Author(s):  
Željka Peršurić ◽  
Lara Saftić Martinović ◽  
Mladenka Malenica ◽  
Ivana Gobin ◽  
Sandra Pedisić ◽  
...  
Keyword(s):  
Biological Activity ◽  
Antiproliferative Activity ◽  
Biological Activities ◽  
Punica Granatum ◽  
Mass Spectrometry Analysis ◽  
Total Phenolic ◽  
Pomegranate Peel ◽  
Spectrometry Analysis ◽  
Ethanol Extracts ◽  
Punica Granatum L

Pomegranate (Punica granatum L.) is a rich source of constituents with confirmed strong biological activities. However, pomegranate peel, which encompasses approximately 30–40% of its weight, is treated as a biological waste. The aim of this paper was to evaluate the potential of pomegranate peel extracts and to propose its functional properties that can be used for development of functional products. Eight ethanol extracts of pomegranate peels (PPEs) were characterized by use of direct infusion quadrupole-time of flight (Q-TOF), and afterwards tested on their antioxidant, antibacterial and antiproliferative activities. Mass spectrometry analysis revealed that the most prevalent compounds in pomegranate peels were punicalagin, granatin and their derivatives. Analysed extracts had high total phenolic contents that ranged from 5766.44 to 10599.43 mg GAE/100 g, and strong antioxidant activity (7551.31–7875.42 and 100.25–176.60 μmol TE/100 g for DPPH and FRAP assays, respectively). The results of biological activity assays showed that all PPEs possessed antibacterial activity, and that S. aureus was the most sensitive specie with minimum inhibitory concentration and minimum bactericidal concentrations ranging from 0.8 to 6.4 mg/mL. Additionally, the analysis of antiproliferative activity revealed high potency of PPEs, as the IC50 values ranged from 0.132 mg/mL to 0.396 mg/mL. Multivariate analysis pointed out the most discriminative metabolites for antioxidant or antiproliferative activity. Overall, the pomegranate peel confirmed to be a highly valuable source of bioactive compounds that could be used to improve the food functional characteristics.

scholarly journals Effects of Harvest Time on Phytochemical Constituents and Biological Activities of Panax ginseng Berry Extracts

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3343 ◽  
Author(s):  
Seung-Yeap Song ◽  
Dae-Hun Park ◽  
Seong-Wook Seo ◽  
Kyung-Mok Park ◽  
Chun-Sik Bae ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Biological Activities ◽  
Mass Spectrometry Analysis ◽  
Harvest Time ◽  
Medicinal Products ◽  
Spectrometry Analysis ◽  
Liquid Chromatography Tandem Mass ◽  
Phytochemical Constituents ◽  
Ethanol Extracts ◽  
Berry Extracts

Ginseng (Panax ginseng) has long been used as a traditional medicine for the prevention and treatment of various diseases. Generally, the harvest time and age of ginseng have been regarded as important factors determining the efficacy of ginseng. However, most studies have mainly focused on the root of ginseng, while studies on other parts of ginseng such as its berry have been relatively limited. Thus, the aim of this study iss to determine effects of harvest time on yields, phenolics/ginsenosides contents, and the antioxidant/anti-elastase activities of ethanol extracts of three- and four-year-old ginseng berry. In both three- and fourfour-year-old ginseng berry extracts, antioxidant and anti-elastase activities tended to increase as berries ripen from the first week to the last week of July. Liquid chromatography-tandem mass spectrometry analysis has revealed that contents of ginsenosides except Rg1 tend to be the highest in fourfour-year-old ginseng berries harvested in early July. These results indicate that biological activities and ginsenoside profiles of ginseng berry extracts depend on their age and harvest time in July, suggesting the importance of harvest time in the development of functional foods and medicinal products containing ginseng berry extracts. To the best of our knowledge, this is the first report on the influence of harvest time on the biological activity and ginsenoside contents of ginseng berry extracts.

scholarly journals Extraction of tannins from Punica granatum peel for pharmacological activities

2020 ◽  
Vol 8 (1) ◽  
pp. 144-147
Author(s):  
Sayed Muhammad Amin Sadat ◽  
Anisa Omari
Keyword(s):  
Biological Activities ◽  
Punica Granatum ◽  
Health Food ◽  
Fruit Peel ◽  
Pomegranate Peel ◽  
Pharmacological Activities ◽  
High Antioxidant Activity ◽  
Anti Inflammatory ◽  
Pomegranate Peel Extract ◽  
Punica Granatum L

Punica granatum L. (Pomegranate) is utilized in the treatment of parasitic contamination, loose bowels and ulcers. Extracts of the various parts of P. granum is reported to have anti-inflammatory, antioxidant and anticarcinogenic activities. Tannins are a group of polyphenolic compounds that are widely present in plant regions and possess various biological activities including antimicrobial, anti-parasitic, anti-viral, antioxidant, anti-inflammatory, immunomodulatory activities. This study focused on extraction of tannins from Punica granatum peel which can be further analyzed for its pharmacological activities. The results of this study revealed the abundance of tannins in the pomegranate peel extract. Tannins from pomegranate peel might contribute to the high antioxidant activity of this fruit peel and it is a valuable natural antioxidant source applicable in the health food industry.

scholarly journals Diversity of Monomers in Nonribosomal Peptides: towards the Prediction of Origin and Biological Activity

2010 ◽  
Vol 192 (19) ◽  
pp. 5143-5150 ◽  
Author(s):  
Ségolène Caboche ◽  
Valérie Leclère ◽  
Maude Pupin ◽  
Gregory Kucherov ◽  
Philippe Jacques
Keyword(s):  
Biological Activity ◽  
Culture Supernatant ◽  
Biological Activities ◽  
Mass Spectrometry Analysis ◽  
Nonribosomal Peptides ◽  
Amino Acid Residues ◽  
Antitumor Activities ◽  
Significant Similarity ◽  
Spectrometry Analysis ◽  
First Time

ABSTRACT Nonribosomal peptides (NRPs) are molecules produced by microorganisms that have a broad spectrum of biological activities and pharmaceutical applications (e.g., antibiotic, immunomodulating, and antitumor activities). One particularity of the NRPs is the biodiversity of their monomers, extending far beyond the 20 proteogenic amino acid residues. Norine, a comprehensive database of NRPs, allowed us to review for the first time the main characteristics of the NRPs and especially their monomer biodiversity. Our analysis highlighted a significant similarity relationship between NRPs synthesized by bacteria and those isolated from metazoa, especially from sponges, supporting the hypothesis that some NRPs isolated from sponges are actually synthesized by symbiotic bacteria rather than by the sponges themselves. A comparison of peptide monomeric compositions as a function of biological activity showed that some monomers are specific to a class of activities. An analysis of the monomer compositions of peptide products predicted from genomic information (metagenomics and high-throughput genome sequencing) or of new peptides detected by mass spectrometry analysis applied to a culture supernatant can provide indications of the origin of a peptide and/or its biological activity.

scholarly journals Determination of Punicalagins Content, Metal Chelating, and Antioxidant Properties of Edible Pomegranate (Punica granatum L) Peels and Seeds Grown in Morocco

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Talal Sabraoui ◽  
Taleb Khider ◽  
Boubker Nasser ◽  
Rabiaa Eddoha ◽  
Abderrahman Moujahid ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Punica Granatum ◽  
Total Phenolic ◽  
Natural Food ◽  
Pomegranate Peel ◽  
Metal Chelating ◽  
Mediterranean Countries ◽  
Punica Granatum L

Pomegranate (Punica granatum L) is widely cultivated in the Mediterranean countries especially in Morocco. Pomegranate peel and seed contain considerable amounts of phenolic compounds with antioxidant activity. The aim of the present study was to phytochemically characterize the pomegranate peels and seeds obtained from three Moroccan provinces, using UHPLC-DAD. In addition, total phenolic content (TPC), total flavonoid contents (TFC), and metal chelating of pomegranate peel were also evaluated. The results showed that pomegranate peel possesses the highest phenolic (TPC: 224.39 mg GAE/g dw) and flavonoid (TFC: 62.64 mg rutin/g dw) contents. Punicalagin-β and punicalagin-α, are the abundant compounds found in peel: 216.36±9.94 mg/g, 154.94±5.21 mg/g, respectively. Pomegranate peels showed significantly (p<0.05) high antioxidant activity 1-diphenyl-2-picrylhydrazyl (DPPH) EC50: 42.71±0.04 μg/mL, 2.2′-Azino-bis(3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) EC50: 62.15±0.01 μg/mL), and chelating activity (FRAP 1.85±0.00 mg ascorbic acid equivalents/100 g, Fe2+: 2.52±0.01 μmol EDTA equivalents/g dw) compared to seeds. A positive correlation between antioxidant activity and total phenolic was found. According to achieved results, high antioxidant capacity of pomegranate extracts, especially peel, shed light to further use as natural food preservatives. Pomegranate peel could be used for the fortification of food with fiber by introducing it in dietary, as well as in health applications due to its higher antioxidant capacity.

scholarly journals Processing Factors Affecting the Phytochemical and Nutritional Properties of Pomegranate (Punica granatum L.) Peel Waste: A Review

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4690
Author(s):  
Tandokazi Pamela Magangana ◽  
Nokwanda Pearl Makunga ◽  
Olaniyi Amos Fawole ◽  
Umezuruike Linus Opara
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Punica Granatum ◽  
Liquid Extraction ◽  
Total Phenolic ◽  
Pomegranate Peel ◽  
Nutritional Properties ◽  
Pressurised Liquid Extraction ◽  
Punica Granatum L ◽  
Processing Factors

Pomegranate peel has substantial amounts of phenolic compounds, such as hydrolysable tannins (punicalin, punicalagin, ellagic acid, and gallic acid), flavonoids (anthocyanins and catechins), and nutrients, which are responsible for its biological activity. However, during processing, the level of peel compounds can be significantly altered depending on the peel processing technique used, for example, ranging from 38.6 to 50.3 mg/g for punicalagins. This review focuses on the influence of postharvest processing factors on the pharmacological, phytochemical, and nutritional properties of pomegranate (Punica granatum L.) peel. Various peel drying strategies (sun drying, microwave drying, vacuum drying, and oven drying) and different extraction protocols (solvent, super-critical fluid, ultrasound-assisted, microwave-assisted, and pressurized liquid extractions) that are used to recover phytochemical compounds of the pomegranate peel are described. A total phenolic content of 40.8 mg gallic acid equivalent (GAE)/g DM was recorded when sun drying was used, but the recovery of the total phenolic content was higher at 264.3 mg TAE/g when pressurised liquid extraction was performed. However, pressurised liquid extraction is costly due to the high initial investment costs and the limited possibility of carrying out selective extractions of organic compounds from complex peel samples. The effects of these methods on the phytochemical profiles of pomegranate peel extracts are also influenced by the cultivar and conditions used, making it difficult to determine best practice. For example, oven drying at 60 °C resulted in higher levels of punicalin of 888.04 mg CE/kg DM compared to those obtained 40 °C of 768.11 mg CE/kg DM for the Wonderful cultivar. Processes that are easy to set up, cost-effective, and do not compromise the quality and safety aspects of the peel are, thus, more desirable. From the literature survey, we identified a lack of studies testing pretreatment protocols that may result in a lower loss of the valuable biological compounds of pomegranate peels to allow for full exploitation of their health-promoting properties in potentially new value-added products.

scholarly journals Chemical Composition and Biological Activities of Seeds from Four Vitis vinifera Tunisian Varieties

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Thouraya Majoul ◽  
Faten Bouabdallah ◽  
Majdi Hammami ◽  
Hajer Satouri ◽  
Olfa Tabbene ◽  
...  
Keyword(s):  
Biological Activities ◽  
Sinapic Acid ◽  
Acid Methyl Ester ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Good Growth ◽  
Spectrometry Analysis ◽  
Beneficial Effects

Thousands of polyphenolic compounds have been identified in various plants. Recently, a number of studies showed that beneficial effects of grapes are related to the presence of polyphenols, with multiple biological activities. The aim of this study was to compare the polyphenol profiles as well as biological activities of the seeds of four Tunisia cultivated grape cultivars, syrat, merlot, cabernet sauvignon and carignan. The total phenolic content of seed extracts varied between varieties and seemed to be correlated to the scavenging activity. Preliminary antibacterial results showed a good growth inhibitory activity of syrat cultivar against Staphylococcus epidermidis. To our knowledge, this is the first of such study being performed on this bacterium, known as a leading cause of Tunisian hospital-acquired infections. Gas Chromatography Mass Spectrometry analysis identified 20 polyphenol components, flavonoids being the most abundant in all extracts, followed by phenolic acids, resveratrol, tyrosol, and syringaldehyde. We also noticed a deficiency of three phenolic acid compounds (salicylic acid methyl ester, ferulic acid, Sinapic acid) as well as a flavonoid one (myricetin) in merlot cultivar, which could be responsible for its low antioxidant activity. These results will help in the selective exploitation of the seeds obtained from winemaking wastes, as well as in further pharmacological and/or in vivo investigations.

scholarly journals Enrichment of Commercially-Prepared Juice With Pomegranate (Punica granatum L.) Peel Extract as a Source of Antioxidants

2014 ◽  
Vol 3 (6) ◽  
pp. 179 ◽  
Author(s):  
Zilmar Meireles Pimenta Barros ◽  
Jocelem Mastrodi Salgado ◽  
Priscilla Siqueira Melo ◽  
Fuvia de Oliveira Biazotto
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Value Added ◽  
Punica Granatum ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Pomegranate Peel ◽  
Physical And Chemical ◽  
Punica Granatum L

<p>Ready-to eat foods meet the demands of a modern lifestyle and the number of people seeking food that is convenient and safe is increasing. The extracts of peels from four different fruits were tested as potential value-added foods to offer to consumers. Physical and chemical analyses of the peel extracts were conducted to measure total phenolic compounds, tannins, phytic acid and antioxidant activity using the 1’-1’Diphenyl-2’picrylhydrazyl, and 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid methods. The result of screening the antioxidant activity showed that the pomegranate peel had higher activity than the other peels (p&lt;0.05). In addition, flavonoids and vitamin C were measured in the pomegranate peel, and low amounts of these components were found. The pomegranate peel had a high amount of phenolic compounds and high levels of antioxidants, and this peel was used to enrich a commercially-available juice. Furthermore, the sensory evaluation showed no difference between the control and enriched juice. The product was well accepted and feasible from a technological standpoint. Because the waste is rich in bioactive compounds, value is added to the final product, as these antioxidant compounds are known to protect health and improve the quality of life of the consumers.</p>

scholarly journals Identification and Quantification of Coumarins by UHPLC-MS in Arabidopsis Thaliana Natural Populations

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1804
Author(s):  
Izabela Perkowska ◽  
Joanna Siwinska ◽  
Alexandre Olry ◽  
Jérémy Grosjean ◽  
Alain Hehn ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
High Performance ◽  
Biological Activities ◽  
Natural Populations ◽  
Biologically Active ◽  
Stress Factors ◽  
Mass Spectrometry Analysis ◽  
Engineering Research ◽  
Spectrometry Analysis ◽  
Model Plant Arabidopsis Thaliana

Coumarins are phytochemicals occurring in the plant kingdom, which biosynthesis is induced under various stress factors. They belong to the wide class of specialized metabolites well known for their beneficial properties. Due to their high and wide biological activities, coumarins are important not only for the survival of plants in changing environmental conditions, but are of great importance in the pharmaceutical industry and are an active source for drug development. The identification of coumarins from natural sources has been reported for different plant species including a model plant Arabidopsis thaliana. In our previous work, we demonstrated a presence of naturally occurring intraspecies variation in the concentrations of scopoletin and its glycoside, scopolin, the major coumarins accumulating in Arabidopsis roots. Here, we expanded this work by examining a larger group of 28 Arabidopsis natural populations (called accessions) and by extracting and analysing coumarins from two different types of tissues–roots and leaves. In the current work, by quantifying the coumarin content in plant extracts with ultra-high-performance liquid chromatography coupled with a mass spectrometry analysis (UHPLC-MS), we detected a significant natural variation in the content of simple coumarins like scopoletin, umbelliferone and esculetin together with their glycosides: scopolin, skimmin and esculin, respectively. Increasing our knowledge of coumarin accumulation in Arabidopsis natural populations, might be beneficial for the future discovery of physiological mechanisms of action of various alleles involved in their biosynthesis. A better understanding of biosynthetic pathways of biologically active compounds is the prerequisite step in undertaking a metabolic engineering research.

Optimization of subcritical water extraction of phenolic antioxidants from pomegranate (Punica granatum L.) peel by response surface methodology

2017 ◽  
Vol 9 (32) ◽  
pp. 4647-4656 ◽  
Author(s):  
Linlin Yan ◽  
Yungang Cao ◽  
Guangyao Zheng
Keyword(s):  
Response Surface Methodology ◽  
Subcritical Water ◽  
Punica Granatum ◽  
Water Extraction ◽  
Phenolic Antioxidants ◽  
Subcritical Water Extraction ◽  
Extraction Technology ◽  
Pomegranate Peel ◽  
Efficient Extraction ◽  
Punica Granatum L

Subcritical water extraction (SWE), a ‘green’ and efficient extraction technology, was applied to extract phenolic antioxidants from pomegranate peel in this study.

Punica granatum peel extracts mediated the green synthesis of gold nanoparticles and their detailed in vivo biological activities

2021 ◽  
Vol 10 (1) ◽  
pp. 882-892
Author(s):  
Sami Bawazeer ◽  
Abdur Rauf ◽  
Taufiq Nawaz ◽  
Anees Ahmed Khalil ◽  
Muhammad Sameem Javed ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Muscle Relaxant ◽  
Biological Activities ◽  
Punica Granatum ◽  
Environmental Safety ◽  
Surface Plasma Resonance ◽  
Pomegranate Peel ◽  
Synthesis Of Nanoparticles ◽  
Vis Spectroscopy

Abstract Requirements for developing new methodologies to biosynthesize nanoparticles are increasing day by day. The typical chemical synthesis of nanoparticles has raised concerns regarding environmental safety and adverse impact on human health. Therefore, there is an urgent need to develop green synthesized nanoparticles that are considered to be safe, ecofriendly, and cost-effective as compared to chemical approaches. Hence, in this study, we synthesized and characterized pomegranate peel extract-based gold nanoparticles (PP-AuNPs) through UV-visible spectroscopy, FT-IR, and AFM microscopy. Furthermore, the biological activities like analgesic, muscle relaxant, and sedative properties of synthesized PP-AuNPs were also determined. The change of color to dark ruby indicates the formation of AuNPs. The surface plasma resonance (SPR) peak in the absorption spectra was shown at 525 nm by using (UV-Vis) spectroscopy. A single distinctive peak implied the shape of nanoparticles to be spherical. AFM images revealed that the biosynthesized nanoparticles were spherical in shape. Furthermore, the images confirm the uniform distribution of PP-AuNPs with particle sizes ranging from 4 to 16 nm. Different classes of phytochemicals were preliminarily identified in extracts. The analgesic effect of extracts (70.04%) and PP-AuNPs (81.98%) demonstrated a significant (p < 0.001) percent reduction in writhing at a dose of 100 and 15 mg·kg−1, respectively. A mild muscle relaxant effect was noted against both the tested samples while a significant sedative effect was observed for both samples; however, PP-AuNPs weres more sedative compared to the extract. Pomegranate peel extracts and synthesized PP-AuNPs were found to possess significant analgesic, muscle relaxant, and sedative properties.

Sign in / Sign up

Export Citation Format

Share Document