Efficacy of Neuroprotection from Curcumin through Heat Shock Protein 70 Induction in Traumatic Brain Injury – Rat Model

BACKGROUND: Traumatic brain injury (TBI) is the most common problem that caused morbidity and mortality in the world. Secondary brain injury is a complex cascade that causes brain cell apoptosis. Curcumin is a natural product that has neuroprotective properties. AIM: This study aimed to investigate the effect of curcumin toward heat shock protein 70 (HSP 70) expression against the expression apoptosis marker (apoptosis-inducing factor [AIF], caspase-3, and TUNEL assay) in brain tissue after TBI. METHODS: Thirty-three Sprague Dawley rats were randomized into three treatment groups, that is, sham-operated controls, closed head trauma (CHT), and CHT with curcumin extract (treatment group). In the treatment group, curcumin was given 500 mg/kg per oral for 7 days, then brain tissues were investigated (marker AIF, caspase-3, TUNEL assay, and HSP 70) through immunohistochemistry. Statistical test using one-way ANOVA test and Tukey honestly significant difference as post hoc test. RESULTS: The mean of positive AIF stained cells in Group A was 5.36 ± 2.11, Group B was 12.82 ± 1.40, and Group C was 3.82 ± 1.40, with a significant difference of AIF expression between Groups C and B (p < 0.05). Mean of positive caspase-3 stained cells in Group A was 5.45 ± 2.30, Group B was 13.82 ± 2.44, and Group C was 3.82 ± 1.54, with a significant difference of caspase-3 expression between Groups C and B (p < 0.05). Mean of positive TUNEL assay stained cells in Group A was 4.82 ± 2.04, Group B was 11.55 ± 1.51, and Group C was 3.55 ± 1.70, with a significant difference between Groups C and B (p < 0.05). Mean of positive HSP 70 stained cells in Group A was 6.82 ± 2.14, Group B was 3.91 ± 2.26, and Group C was 10.27 ± 2.45 with a significant difference of HSP 70 expression distribution within groups (p < 0.05). CONCLUSION: Curcumin may protect brain cells from apoptosis after close head trauma by upregulated HSP 70 expression.

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EFFECT OF KALO JEERA SEEDS AND PAPAYA LEAF SUPPLEMENTATION ON THE PERFORMANCE OF BROILER

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v14i1.28820 ◽

2016 ◽

Vol 14 (1) ◽

pp. 37-42 ◽

Cited By ~ 3

Author(s):

M. G. Sorwar ◽

M. Mostofa ◽

M. N. Hasan ◽

M. Billah ◽

M. T. Rahman

Keyword(s):

Body Weight ◽

Treatment Group ◽

Nigella Sativa ◽

Control Group ◽

Growth Promoter ◽

Final Body Weight ◽

Live Body Weight ◽

Significant Difference ◽

Group A ◽

Group B

This experiment was conducted to determine the effect of papaya leaf (Carica papaya) and kalo jeera (Nigella sativa) seeds powdered supplementation in drinking water as a growth promoter in broiler chickens. A total of 20 Cobb-500 broiler chicks (day-old) were purchased from local hatchery (Nourish Poultry and Hatchery Ltd.) and after seven days of acclimatization chicks were randomly divided into two groups, A (n=10) and B (n=10). The group A was kept as a control and not treated. The group B was supplemented with papaya leaf and kalo jeera powder with feed and water. Weekly observations were recorded for live body weight gain up to 5th weeks and hematological tests were performed at 35th days age of broiler to search for hematological changes between control (A) and treatment (B) groups. The initial body weight of groups A and B on 1st were 41.00±0.56 gm and 41.50±0.35 gm, respectively and after 35th day of experiment final body weight were 1470±57.35 gm and 1720±58.56 gm, respectively and economics of production were analyzed and found that net profit per broiler was Tk. 8.91 and Tk. 20.69, respectively. The treatment group B was recorded statistically significant (at 1% level) increased (17.00%) for live body weight than that of control group A. The hematological parameters total erythrocyte count (TEC), erythrocyte sedimentation rate (ESR) and hemoglobin (Hb) estimation value of treatment group shows significant difference, while hemoglobin estimation does not show significant difference from control group. The results suggest that better growth performance could be achieved in broilers supplemented with papaya leaf and kalo jeera seeds.

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EFFECT OF TOCOPHEROL AND SELENIUM ON THE PERFORMANCE OF BUSERELIN FOR ESTRUS INDUCTION IN LATE SEASON ANESTRUS MARES (EQUUS CABALLUS)

Agrobiological Records ◽

10.47278/journal.abr/2020.022 ◽

2021 ◽

Vol 4 ◽

pp. 8-13

Author(s):

Farah Ali ◽

Laeeq Akbar Lodi ◽

Syed Saad Ul Hazzan ◽

Riaz Hussain

Keyword(s):

Treatment Group ◽

Blood Serum ◽

Gnrh Analogue ◽

Progesterone Concentration ◽

Late Season ◽

Significant Difference ◽

Daily Dose ◽

Group A ◽

Group B ◽

Follicular Dynamics

The aim of this study was to determine the effect of tocopherol and selenium on the performance of buserelin (GnRH analogue) for estrus induction in anestrus mares. A total of 18 seasonally anestrus mares were divided into three equal groups (n=6 in each) after ovarian scanning. Mares in group A (treatment group) were treated with tocopherol and selenium intramuscularly along with daily dose of buserelin. Mares which came into heat within ten-day buserelin protocol, further injections of buserelin for those mares were stopped. Mares in group B were treated with buserelin intramuscularly only for ten days. Mares which came into heat within ten-day buserelin protocol, further injections of buserelin were stopped. Blood/serum was collected from each experimental mare kept in different groups (A-C) at days 1, 11, 12, 13 and 14. Onset of estrus signs was 83.3, 33.3 and 0% in group A, B and C, respectively, that was significant (P<0.05) among groups. Follicular dynamics were 20.24±3.00, 14.59±2.24 and 10.35±0.63mm in groups A, B and C, respectively with significant (P˂0.05) difference. Change in serum E2 contents was significantly (P˂0.05) different among groups A, B and C whereas progesterone concentration showed non-significant difference among groups.

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Effect of Different Hemodialysis Methods on Microbiota in Uremic Patients

BioMed Research International ◽

10.1155/2020/6739762 ◽

2020 ◽

Vol 2020 ◽

pp. 1-8

Author(s):

Haidong He ◽

Yan Xie

Keyword(s):

Escherichia Coli ◽

Treatment Group ◽

Enterococcus Faecalis ◽

Lactobacillus Acidophilus ◽

Intestinal Flora ◽

Significant Difference ◽

Group A ◽

Uremic Patients ◽

Group B ◽

Esrd Patients

Background. To investigate the effect of hemodialysis on microbiota in uremic patients. Objective. To investigate the effect of hemodialysis on microbiota in uremic patients. Methods. This study included 85 adult patients who have received hemodialysis since August 2014, and the treatment plan has not changed for more than 12 months. These patients were divided into hemodialysis group (group A), hemodialysis+hemodialysis filtration group (group B), and hemodialysis+hemodialysis filtration+blood perfusion group (group C). Twenty-four adult ESRD patients (CK group) were enrolled. Serum biochemical indexes were measured, glomerular filtration rate (EGFR) was estimated, dialysis adequacy (kt/V) was calculated, and fresh feces were collected. At the same time, the feces of 30 health workers were selected as the control. 16S rRNA sequence was used to determine the intestinal flora of all fecal specimens. First of all, we analyzed the difference of the whole flora distribution between dialysis and nondialysis ESRD patients; then, we selected the most representative content of bifidobacteria, Lactobacillus acidophilus, Escherichia coli, and Enterococcus faecalis to analyze the influence of different blood purification methods on the intestinal flora. Results. (1) The level of C-reactive protein (CRP) in dialysis patients was lower than that in nondialysis ESRD patients, and CRP in group C was lower than that in groups A and B. There was no significant difference in kt/V between group A, group B, and group C. There was no significant difference in EGFR between the four groups. (2) The species diversity of ESRD patients without dialysis (CK group) was significantly lower than that of ESRD patients with dialysis; there was no significant difference between group A and group B; the species diversity of group C was significantly higher than that of group A and group B. (3) Compared with the control group, the levels of bifidobacteria and Lactobacillus acidophilus in ESRD patients were significantly lower, while the levels of Escherichia coli and Enterococcus faecalis were significantly higher. (4) The levels of bifidobacteria and Lactobacillus acidophilus in hemodialysis patients were significantly higher than those in nonblood purification treatment group, and the levels of Escherichia coli and Enterococcus faecalis were significantly lower than those in nonblood purification treatment group. (5) The level of Lactobacillus acidophilus in group C was significantly higher than that in groups A and B, and the level of Escherichia coli was significantly lower than that in groups A and B. Conclusion. ESRD patients have microbiota disorder. Hemodialysis can improve microbiota disorder in uremic patients. Compared with ordinary hemodialysis, combined hemoperfusion dialysis can further improve microbiota disorder.

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Relationship between Dizziness and the Core Vestibular Projection Injury in Patients with Mild Traumatic Brain Injury

Diagnostics ◽

10.3390/diagnostics11112070 ◽

2021 ◽

Vol 11 (11) ◽

pp. 2070

Author(s):

Sung-Ho Jang ◽

Chang-Hoon Bae ◽

Jae-Woon Kim ◽

Hyeok-Gyu Kwon

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

Mild Traumatic Brain Injury ◽

Injury Severity ◽

Diffusion Tensor ◽

Control Group ◽

Item Score ◽

Mild Tbi ◽

Group A ◽

Group B

Some studies have reported that a core vestibular projection (CVP) injury is associated with dizziness following a brain injury using diffusion tensor tractography (DTT). On the other hand, there has been no DTT study on dizziness caused by a CVP injury in patients with mild traumatic brain injury (TBI). In this study, DTT was used to examine the relationship between dizziness and CVP injury in patients with mild TBI. Forty-three patients with mild TBI and twenty-nine normal subjects were recruited. The patients were classified into two groups based on the dizziness score: group A, patients with a dizziness score less than 2 on the sub-item score for dizziness in the Rivermead Post-concussion Symptoms Questionnaire; group B, patients with a dizziness score above 2. The tract volume (TV) in group B was significantly lower than group A and the control group (p < 0.05). By contrast, the TV in group A was similar to the control group (p > 0.05). Regarding the correlation, the dizziness score of all patients showed a strong negative correlation with the TV of the CVP (r = −0.711, p < 0.05). DTT revealed the CVP injury in patients with dizziness after mild TBI. In addition, the severity of dizziness of these patients was closely related to the injury severity of the CVP.

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HUBUNGAN OKSIDA NITRAT DAN NILAI HISTOPATOLOGIS PADA ENDOTOKSEMIA

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v20i3.467 ◽

2016 ◽

Vol 20 (3) ◽

pp. 201

Author(s):

Sotianingsih Sotianingsih ◽

Suharyo Suharyo ◽

Lisyani S ◽

Guntur HA

Keyword(s):

Nitric Oxide ◽

Treatment Group ◽

Control Group ◽

Significant Difference ◽

Group A ◽

The Mean ◽

Group B ◽

Termination Time ◽

Control Group Group

Lipopolysaccharide (LPS) is an endotoxin from the outer membrane of Gram-negative bacteria, which has an important role in the occurrence of sepsis. Exposure to LPS will stimulate increase of nitric oxide (NO). Nitric oxide is a bioregulator of apoptosis and has some sepsis prognostic role of apoptosis regulators within the gastrointestinal cells. The objective of the study is to know if endotoxemia induces an increase in NO levels and histopathology scores as well as the existing relationship between them. This study is an observational intervention. The subjects were 48 male mice Balb/C, divided into 2 groups. The samples consisted of 24 tail as control group (group A) and 24 as treatment group (group B). The A group as well as the B group is divided into 4 subgroups according to the time of termination. The levels of NO were examined by Griess method. Histopathology score was examined by HE and read as a score of 0–5. There was a statistically significant difference between the mean NO in the treatment group with the control group at the termination of the group of 12h (p=0.009), 24h (p=0.015), 36h (p=0.014), 48h (p=0.002) and the whole group (p=0.0001), as well as between the mean histopathology score at the termination time of 12 h (p=0.0001), 24h (p=0.0001), 36h (p=0.0001), 48h (p=0465) and the whole group (p=0.0001). Increase in NO and histopathology scores in all groups of mice (r=0.527) showed a statistically significant correlation. NO levels and histopathology scores are increased during endotoxemia and thus have a significant correlation.

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A clinical practice evaluating the more appropriate airway humidification methods applied on patients with severe traumatic brain injury

10.21203/rs.3.rs-16815/v1 ◽

2020 ◽

Author(s):

xiaohong yin ◽

Banyou MA ◽

Lei YANG ◽

Hua SUN ◽

Zhilei YUN ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

Severe Traumatic Brain Injury ◽

Secondary Infection ◽

Formation Rate ◽

Nursing Workload ◽

Airway Humidification ◽

Group A ◽

Group B ◽

Heat And Moisture

Abstract Airway humidification is an essential treatment for severe traumatic brain injury (STBI) patients after tracheotomy. To date, there was no relevant quantitative study evaluating these humidification ways and providing the appropriate method for the long-term nursing of these patients. In this study, 150 patients whom received tracheotomy treatment in our hospital from January 2016 to November 2018 were recruited into this study. Subjects were divided into three groups according to the humidification way that they received. The groups were oxygen spraying group (group A), Heat and moisture exchanger (HME) group (group B) and the heating and humidification group (group C). Phlegm viscosity, humidification effect, phlegm formation rate, daily sputum inhalation times, airway spasm, secondary lung infection, daily nursing times, nurses' internal satisfaction were evaluated. The C method is superior to both A and B methods in most aspects. A method trends to happening with insufficient or excessive humidification. Phlegm scab formation is significantly less in group C. The B and C methods had equal humidification effects and need similar daily sputum inhalation nursing. Airway spasm was frequent happened in group A than that in groups B and C. Secondary infection happened in all groups, the number of infected patients showed a decreasing trend and the infection degree showed no difference in the first 7 days between group C and group A. And the severity but not the infection ratio significantly decreased in Group C on the 30 th day. Method A significantly reduced nursing workload, but gained the worst humidification effects. Collectively, C method is more suitable for the airway nursing of patients with severe traumatic brain injury.

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Selective hypothermia: an experimental study on traumatic brain injury in rats

Arquivos de Neuro-Psiquiatria ◽

10.1590/s0004-282x2008000300021 ◽

2008 ◽

Vol 66 (2b) ◽

pp. 391-396 ◽

Cited By ~ 1

Author(s):

Amylcar Edemilson Dvilevicius ◽

Mirto Nelso Prandini

Keyword(s):

Traumatic Brain Injury ◽

Experimental Study ◽

Brain Injury ◽

Control Group ◽

Histopathological Analysis ◽

Group A ◽

Cortical Impact ◽

The Difference ◽

Group B ◽

The Right

OBJECTIVE: To evaluate the efficiency of selective hypothermia in the treatment of the traumatic brain injury in rats. METHOD: After the trauma produced for the model of cortical impact, a small craniectomy in the right frontoparietal region was carried through; after the procedure the animals had been divided in two groups of 15 each. Group A, without treatment with hypothermia (control group) and group B, treated with selective hypothermia for a period to 5 to 6 hours. After this time all the animals were sacrificed, their brains had been removed and histopathological analysis was carried through. RESULTS: Comparison between both groups was done using the counting of neurons injured for field. Counting in the control group n=15 had an average of 70.80 neurons injured for field against an average of 21.33 neurons injured for field in group B (submitted to the treatment with hypothermia), with n=15 also. The difference was statiscally significant. CONCLUSION: Based in the quantification of the neurons injured for field, the effectiveness of the treatment with selective hypothermia was demonstrated.

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EFFECTS OF TULSI LEAF EXTRACT ON BODY WEIGHT GAIN IN BROILER PRODUCTION

Bangladesh Journal of Veterinary Medicine ◽

10.3329/bjvm.v14i1.28818 ◽

2016 ◽

Vol 14 (1) ◽

pp. 21-25 ◽

Cited By ~ 2

Author(s):

M. N. Hasan ◽

M. Mostofa ◽

M. G. Sorwar ◽

M. T. Hasan ◽

K. Das ◽

...

Keyword(s):

Body Weight ◽

Weight Gain ◽

Treatment Group ◽

Leaf Extract ◽

Body Weight Gain ◽

Control Group ◽

Live Body Weight ◽

Significant Difference ◽

Group A ◽

Group B

The objective of this research was to evaluate the effect of tulsi leaf (Ocimum sanctum) extract supplementation in drinking water as a growth promoter in broiler chickens. A total of 40 Cobb-500 broiler chicks (day-old) were purchased from local hatchery (Nourish Poultry and Hatchery Ltd.). After seven days of acclimatization chicks were randomly divided into two groups, A (n=20) and B (n=20). The group A was kept as a control and not treated. The group B was supplemented with tulsi leaf extract with feed and water. Weekly observations were recorded for live body weight gain up to 5th weeks and hematological tests were performed at 35th days age of broiler to search for hematological changes between control (A) and treatment (B) groups. The initial body weight of groups A and B on 1st day of this experiment were 41.00±1.78 gm and 41.50±2.35 gm, respectively and after 35th day of experiment final body weight were 1640± 74.83 gm and 1920± 33.91 gm, respectively and economics of production were analyzed and found that net profit per broiler was Tk. 21.46 and Tk. 32.29, respectively. The treatment group B was recorded statistically significant (at 1% level) increased (16.97%) for live body weight than that of control group A. The hematological parameters total erythrocyte count (TEC), erythrocyte sedimentation rate (ESR), packed cell volume (PCV) and hemoglobin (Hb) estimation value of treatment group shows significant difference, while hemoglobin (Hb) estimation does not show significant difference from control group. The results suggest that better growth performance could be achieved in broilers supplemented with tulsi leaf extract.

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Efficacy as a growth promoter of Tulsi leaves extract in broiler production with carcass characteristics and hematological profile

World Journal of Advanced Research and Reviews ◽

10.30574/wjarr.2021.11.3.0452 ◽

2021 ◽

Vol 11 (3) ◽

pp. 272-279

Author(s):

Md Tahidul Islam ◽

Md Ashraf Zaman Faruk ◽

Md Sazzad Hossain ◽

Dolan Das

Keyword(s):

Body Weight ◽

Weight Gain ◽

Treatment Group ◽

Body Weight Gain ◽

Control Group ◽

Growth Promoter ◽

Live Body Weight ◽

Significant Difference ◽

Group A ◽

Group B

This experiment was conducted to investigate the efficacy of tulsi leaves (Ocimum sanctum) extract supplementation in drinking water as a growth promoter in broiler chickens. A total No. of 30 day old Cobb-500 broiler chicks was purchased from local hatchery and after seven days of acclimatization chicks were randomly divided into two groups A and B in equal number. The group A was kept as a control and not treated. The group B was supplemented with tulsi leaves extract with feed and water. Weekly observations were recorded for live body weight gain up to 30 days and hematological tests were performed at 30th day’s age of broiler to evaluate the hematological changes between two groups. The initial body weight of groups A and B on 1st day of this experiment were 42.80±10.31 gm and 42.68±6.90 gm respectively and after 30th days of experiment final body weight were 1530± 33.07 gm and 1600± 44.80 gm respectively; the net body weight gain from 7th day were 1488±41.12 gm and 1551±39.29 gm respectively and economics of production was analyzed and found that net profit per broiler was Tk. 24.51 and Tk. 32.50 respectively. The treatment group B was reported statistically significant (at 1% level) increased 16.43% for live body weight than the control group A. The hematological parameters TEC, ESR and PCV value of treatment group showed significant difference, while Hemoglobin (Hb) estimation did not show significant difference from control group. Therefore, the findings of the study results suggest that supplemented with tulsi leaves extract with feed and water can improve the growth performance of broiler.

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Protective Effects of Propolis Extract in a Rat Model of Traumatic Brain Injury via Hsp70 Induction

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2019.736 ◽

2019 ◽

Vol 7 (17) ◽

pp. 2763-2766 ◽

Cited By ~ 1

Author(s):

Steven Tandean ◽

Iskandar Japardi ◽

Michael Lumintang Loe ◽

Wibi Riawan ◽

Julius July

Keyword(s):

Traumatic Brain Injury ◽

Brain Injury ◽

Caspase 3 ◽

Brain Cell ◽

Hsp70 Expression ◽

Propolis Extract ◽

Significant Difference ◽

Group 3 ◽

Group 2 ◽

Group 1

BACKGROUND: Traumatic brain injury (TBI) is one of the major global health problems. Secondary brain injury is a complex inflammation cascades process that causes brain cell apoptosis. Propolis is a natural product that has neuroprotective property. AIM: This study aimed to investigate the effect of propolis toward Hsp70 expression with apoptosis marker in brain tissue after TBI. METHODS: Thirty-three Sprague Dawley rats were randomised into three treatments group, i.e. sham-operated controls, closed head injury (CHI), and CHI with propolis extract (treatment group). In the treatment group, propolis was given 200 mg/kg per oral for 7 days then harvested brain tissues after sacrificed by cervical dislocation at day 8. We investigated Hsp70, Caspase 3, apoptosis-inducing factor (AIF), and TUNEL assay expression using immunohistochemistry staining. Statistical test using one-way ANOVA test and Tukey HSD as post hoc test. RESULTS: Mean of positive Hsp70 stained cells in group 1 was 6.82 ± 2.14, group 2 was 3.91 ± 2.26, and group 3 was 9.64 ± 3.53 with a significant difference of Hsp70 expression distribution within groups (p = 0.0001). Mean of positive caspase 3 stained cells in group 1 was 5.45 ± 2.30, group 2 was 13.82 ± 2.44, and group 3 was 7.03 ± 1.54 with a significant difference of caspase3 expression distribution within groups (p=0.0001). Mean of positive AIF stained cells in group 1 was 5.36 ± 2.11, group 2 was 12.82 ± 1.40, and group 3 was 8.09 ± 1.81 with a significant difference of AIF expression distribution within groups (p = 0.0001). Mean of positive TUNEL assay stained cells in group 1 was 4.82 ± 2.04, group 2 was 11.55 ± 1.51, and group 3 was 7.64 ± 1.96 with a significant difference of TUNEL test expression distribution within groups (p = 0.0001). CONCLUSION: Propolis may protect brain cell from apoptosis after injury by maintaining Hsp70 expression in addition to antioxidant and anti-inflammatory.

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