Comparison of ferulic acid content in Radix Angelicae Sinensis, Danggui-Buxue-Tang and Danggui-Sini-Tang

Danggui Buxue Tang (DBT), a Chinese herbal decoction used to treat ailments in women, contains Radix Astragali (Huangqi; RA) and Radix Angelicae Sinensis (Danggui; RAS). When DBT was applied onto cultured MG-63 cells, an increase of cell proliferation and differentiation of MG-63 cell were revealed: both of these effects were significantly higher in DBT than RA or RAS extract. To search for the biological markers that are specifically regulated by DBT, DNA microarray was used to reveal the gene expression profiling of DBT in MG-63 cells as compared to that of RA- or RAS-treated cells. Amongst 883 DBT-regulated genes, 403 of them are specifically regulated by DBT treatment, including CCL-2, CCL-7, CCL-8, and galectin-9. The signaling cascade of this DBT-regulated gene expression was also elucidated in cultured MG-63 cells. The current results reveal the potential usage of this herbal decoction in treating osteoporosis and suggest the uniqueness of Chinese herbal decoction that requires a well-defined formulation. The DBT-regulated genes in the culture could serve as biological responsive markers for quality assurance of the herbal preparation.

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The Chinese Herbal Decoction Danggui Buxue Tang Inhibits Angiogenesis in a Rat Model of Liver Fibrosis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/284963 ◽

2012 ◽

Vol 2012 ◽

pp. 1-11 ◽

Cited By ~ 16

Author(s):

Jing Lv ◽

Zhimin Zhao ◽

Yuan Chen ◽

Qinglan Wang ◽

Yanyan Tao ◽

...

Keyword(s):

Liver Fibrosis ◽

Rat Model ◽

Sod Activity ◽

Danggui Buxue Tang ◽

Radix Angelicae Sinensis ◽

Chinese Herbal ◽

Gene And Protein Expression ◽

Herbal Decoction ◽

Angiogenic Effect ◽

Angelicae Sinensis

In this study, we investigated the anti-angiogenic effect of the Chinese herbal decoction Danggui Buxue Tang (DBT;Radix AstragaliandRadix Angelicae sinensisin 5 : 1 ratio) in a rat model of liver fibrosis, in order to elucidate its mechanisms of action against liver fibrosis. Liver fibrosis was induced with CCl4and high-fat food for 6 weeks, and the rats were treated with oral doses of DBT (6 g raw herbs/kg/d) and N-Acetyl-L-cysteine (NAC; 0.1 g/kg/d). The results showed that both DBT and NAC attenuated liver fibrosis and neo-angiogenesis. Furthermore, DBT and NAC improved SOD activity but decreased MDA content and 8-OH-dG in fibrotic livers, with DBT being more effective than NAC. DBT decreased the expression of VEGF, Ang1 and TGF-β1 and their signaling mediators, whereas NAC had no effect on VEGF and VEGFR2 expression. Both DBT and NAC reduced HIF-1αgene and protein expression in fibrotic livers, with DBT being more effective. These data clearly demonstrate that the anti-fibrotic properties of DBT are related to its ability to inhibit angiogenesis and its anti-angiogenic mechanisms are associated with improving oxidative stress, regulating the expression and signaling of angiogenic factors, and especially modulating HIF-1αin fibrotic livers.

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Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model

Journal of Immunology Research ◽

10.1155/2017/9786972 ◽

2017 ◽

Vol 2017 ◽

pp. 1-12 ◽

Cited By ~ 9

Author(s):

Jingwei Zhou ◽

Xue Li ◽

Peiying Deng ◽

Yi Wei ◽

Juan Liu ◽

...

Keyword(s):

Stem Cells ◽

Herbal Formula ◽

Radix Astragali ◽

Hematopoietic Stem ◽

Danggui Buxue Tang ◽

Chinese Herbal Formula ◽

Radix Angelicae Sinensis ◽

Chinese Herbal ◽

Immune Mediated ◽

Angelicae Sinensis

A derivative formula, DGBX, which is composed of three herbs (Radix astragali,Radix Angelicae sinensis, andCoptis chinensisFranch), is derived from a famous Chinese herbal formula, Danggui Buxue Tang (DBT) (Radix astragaliandRadix Angelicae sinensis). We aimed to investigate the effects of DGBX on the regulation of the balance between proliferation and apoptosis of hematopoietic stem cells (HSCs) due to the aberrant immune response in a mouse model of aplastic anemia (AA). Cyclosporine (CsA), an immunosuppressor, was used as the positive control. Our results indicated that DGBX could downregulate the production of IFNγin bone marrow cells by interfering with the binding between SLAM and SAP and the expressions of Fyn and T-bet. This herbal formula can also inhibit the activation of Fas-mediated apoptosis, interferon regulatory factor-1-induced JAK/Stat, and eukaryotic initiation factor 2 signaling pathways and thereby induce proliferation and attenuate apoptosis of HSCs. In conclusion, DGBX can relieve the immune-mediated destruction of HSCs, repair hematopoietic failure, and recover the hematopoietic function of HSCs in hematogenesis. Therefore, DGBX can be used in traditional medicine against AA as a complementary and alternative immunosuppressive therapeutic formula.

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The expression of erythropoietin triggered by Danggui Buxue Tang, a Chinese herbal decoction prepared from Radix Astragali and Radix Angelicae Sinensis, is mediated by the hypoxia-inducible factor in cultured HEK293T cells

Journal of Ethnopharmacology ◽

10.1016/j.jep.2010.08.029 ◽

2010 ◽

Vol 132 (1) ◽

pp. 259-267 ◽

Cited By ~ 42

Author(s):

Ken Y.Z. Zheng ◽

Roy C.Y. Choi ◽

Heidi Q.H. Xie ◽

Anna W.H. Cheung ◽

Ava J.Y. Guo ◽

...

Keyword(s):

Hypoxia Inducible Factor ◽

Radix Astragali ◽

Danggui Buxue Tang ◽

Radix Angelicae Sinensis ◽

Chinese Herbal ◽

Herbal Decoction ◽

Angelicae Sinensis

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A Chinese Herbal Decoction, Danggui Buxue Tang, Prepared from Radix Astragali and Radix Angelicae Sinensis Stimulates the Immune Responses

Planta Medica ◽

10.1055/s-2006-947186 ◽

2006 ◽

Vol 72 (13) ◽

pp. 1227-1231 ◽

Cited By ~ 52

Author(s):

Qiu Gao ◽

Jerry Cheung ◽

Jun Li ◽

Glanice Chu ◽

Ran Duan ◽

...

Keyword(s):

Immune Responses ◽

Radix Astragali ◽

Danggui Buxue Tang ◽

Radix Angelicae Sinensis ◽

Chinese Herbal ◽

Herbal Decoction ◽

Angelicae Sinensis

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EFFECTS OF “HOE HOA TAN II” REMEDY IN THE TREATMENT OF INTERNAL HAEMORROHIDS GRADE I, II AND III

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2015.2.13 ◽

2015 ◽

pp. 80-85

Author(s):

Van Anh Nguyen ◽

Van Nhan Le ◽

Nguyen Nhu Phuong Phan

Keyword(s):

Side Effects ◽

Key Words ◽

Patient Group ◽

Controlled Study ◽

Therapeutic Effects ◽

Radix Angelicae Sinensis ◽

Randomised Controlled Study ◽

Citrus Deliciosa ◽

Randomised Controlled ◽

Angelicae Sinensis

Objectives: To investigate and evaluate the therapeutic effects of Hoe hoa tan II in the treatment of internal haemorroids grade I, II and III. Ingredients of the remedy include: Hoe Hoa (Styphonolonium japonicum), Chi xac (Fructus citri Aurantii), Hau phac (Magnolia offinalis), Tran bi (Citrus deliciosa Tenore), Thuong thuat (Atractylodes lancea), O mai (Armeniaca vulgaris Lamk), Cam thao (Clycyrrhiza uralensis), Duong quy (Radix Angelicae Sinensis). Subjects and methods: Randomised controlled study (RCT) has been conducted on 60 patients which were divided into two groups, i.e. the first 30 patient group were treated with Hoe hoa tan II remedy 20 g, and the second 30 patient group were treated with Daflon 500 mg in the course of 14 days. Results: Study showed that Hoe hoa tan II has helped improve symptoms of internal haemorroids grade I, II and III such as bleeding, anal exudation, pain, reducing the size of the haemorroid tissues. The remedy has been shown to have the most significant effect on relieving constipation which is typical in haemorroids. The study also revealed no unwanted effects caused by this formula. Conclusion: Hoe hoa tan II can be therapeutically used to treat internal haemorroids grade I, II and III without causing any serious side effects. Key words: Hoe hoa tan II, internal haemorroids grade I, II, III.

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