scholarly journals Dietary flavonoid fisetin targets caspase-3-deficient human breast cancer MCF-7 cells by induction of caspase-7-associated apoptosis and inhibition of autophagy

2011 ◽  
Author(s):  
Shu-Jun Chiu
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Caspase 3 ◽  
Human Breast ◽  
Caspase 7 ◽  
Dietary Flavonoid ◽  
Mcf 7

scholarly journals Proapoptotic and Antiproliferative Effects ofThymus caramanicuson Human Breast Cancer Cell Line (MCF-7) and Its Interaction with Anticancer Drug Vincristine

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Saeed Esmaeili-Mahani ◽  
Farzaneh Falahi ◽  
Mohammad Mehdi Yaghoobi
Keyword(s):  
Breast Cancer ◽  
Cyclin D1 ◽  
Cancer Cells ◽  
Anticancer Drug ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Caspase 3 ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Mcf 7

Thymus caramanicus Jalasis one of the species of thymus that grows in the wild in different regions of Iran. Traditionally, leaves of this plant are used in the treatment of diabetes, arthritis, and cancerous situation. Therefore, the present study was designed to investigate the selective cytotoxic and antiproliferative properties ofThymus caramanicusextract (TCE). MCF-7 human breast cancer cells were used in this study. Cytotoxicity of the extract was determined using MTT and neutral red assays. Biochemical markers of apoptosis (caspase 3, Bax, and Bcl-2) and cell proliferation (cyclin D1) were evaluated by immunoblotting. Vincristine was used as anticancer control drug in extract combination therapy. The data showed that incubation of cells with TCE (200 and 250 μg/mL) significantly increased cell damage, activated caspase 3 and Bax/Bcl2 ratio. In addition, cyclin D1 was significantly decreased in TCE-treated cells. Furthermore, concomitant treatment of cells with extract and anticancer drug produced a significant cytotoxic effect as compared to extract or drugs alone. In conclusion, thymus extract has a potential proapoptotic/antiproliferative property against human breast cancer cells and its combination with chemotherapeutic agent vincristine may induce cell death effectively and be a potent modality to treat this type of cancer.

scholarly journals Corn Silk (Zea mays L.) Induced Apoptosis in Human Breast Cancer (MCF-7) Cells via the ROS-Mediated Mitochondrial Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Mai M. Al-Oqail ◽  
Ebtesam S. Al-Sheddi ◽  
Nida N. Farshori ◽  
Shaza M. Al-Massarani ◽  
Eman A. Al-Turki ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Caspase 3 ◽  
Mitochondrial Pathway ◽  
Ros Production ◽  
Human Breast ◽  
Caspase 9 ◽  
Corn Silk ◽  
Induced Apoptosis ◽  
Mcf 7

Cancer has been recognized as one of the life-threating diseases. Breast cancer is a leading cause of mortality among women. In spite of current developments in the therapy and diagnosis of cancer, the survival rate is still less. Recently, plant-derived natural products gain attention as anticancer agents due to the nontoxic nature. Therefore, the aim of present study was to investigate the anticancer capacity of corn silk extract (CSE) on human breast cancer (MCF-7) and normal human mesenchymal (hMSC-TERT4) cells. Following 24 h treatment to corn silk extract, the cytotoxicity was assessed by MTT, NRU, and morphological assays. The oxidative stress markers (GSH and LPO), ROS production, MMP change, and expression of apoptotic marker genes (p53, Bax, Bcl-2, caspase-3, and caspase-9) were also studied in MCF-7 cells treated at 250 to 1000 μg/ml of CSE for 24 h. Our results showed that CSE decreased the cell viability and increased the apoptosis in a dose-dependent manner. The level of LPO and ROS production was found significantly higher; however, GSH and MMP level was observed lower in CSE-treated MCF-7 cells. The real-time PCR data showed a significant upregulation in p53, Bax, caspase-3, and caspase-9 and downregulation in the mRNA expression of Bcl-2 genes in MCF-7 cells exposed to CSE. Collectively, the data from this study stated that corn silk extract induced apoptosis via the ROS-mediated mitochondrial pathway in MCF-7 cells.

Apigenin, a dietary flavonoid, induces apoptosis, DNA damage, and oxidative stress in human breast cancer MCF-7 and MDA MB-231 cells

2018 ◽  
Vol 391 (5) ◽  
pp. 537-550 ◽  
Author(s):  
Ivana Vrhovac Madunić ◽  
Josip Madunić ◽  
Maja Antunović ◽  
Mladen Paradžik ◽  
Vera Garaj-Vrhovac ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Dna Damage ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Dietary Flavonoid ◽  
And Oxidative Stress ◽  
Mcf 7

scholarly journals Downregulation of KDR expression induces apoptosis in breast cancer cells

2014 ◽  
Vol 19 (4) ◽  
Author(s):  
Xiao Zhang ◽  
Yin-Lin Ge ◽  
Shu-Ping Zhang ◽  
Ping Yan ◽  
Run-Hua Tian
Keyword(s):  
Breast Cancer ◽  
Cytochrome C ◽  
Membrane Permeability ◽  
Human Breast Cancer ◽  
Mitochondrial Membrane ◽  
Caspase 3 ◽  
Human Breast ◽  
Cytochrome C Release ◽  
Mitochondrial Membrane Permeability ◽  
Mcf 7

AbstractAngiogenesis plays a crucial role in the growth, invasion and metastasis of breast cancer. Vascular endothelial growth factors (VEGFs) and their receptors (VEGFRs) are the key regulators of tumor angiogenesis. VEGFR-2, known as the kinase insert domain receptor (KDR), is a key receptor involved in malignant angiogenesis. We previously showed that knocking down KDR with short interference RNA (KDR-siRNA) markedly decreased KDR expression and suppressed tumor growth in a xenograft model. However, the mechanisms underlying the anti-cancer effects of KDR-siRNA are not clearly understood. This study aimed to elucidate the molecular mechanisms that induce apoptosis in human breast cancer MCF-7 cells after transfection with KDR-siRNA. We studied the effects of KDR-siRNA on proliferation, apoptosis, antiapoptotic and pro-apoptotic proteins, mitochondrial membrane permeability, cytochrome c release and caspase-3 activity. The results indicated that KDR-siRNA treatment significantly inhibited the proliferation and induced the apoptosis of MCF-7 cells, reduced the levels of the anti-apoptotic proteins, Bcl-2 and Bcl-xl, and increased the level of the pro-apoptotic protein Bax, resulting in a decreased Bcl-2/Bax ratio. KDR-siRNA also enhanced the mitochondrial membrane permeability, induced cytochrome c release from the mitochondria, upregulated apoptotic protease-activating factor-1 (Apaf-1), cleaved caspase-3, and increased caspase-3 activity in MCF-7 cells. Furthermore, KDR-siRNA-induced apoptosis in MCF-7 cells was blocked by the caspase inhibitor Z-VAD-FMK, suggesting a role of caspase activation in the induction of apoptosis. These results indicate that the Bcl-2 family proteins and caspase-related mitochondrial pathways are primarily involved in KDR-siRNAinduced apoptosis in MCF-7 cells and that KDR might be a potential therapeutic target for human breast cancer treatments.

scholarly journals Cytotoxic and Apoptotic Effects of Govaniadine Isolated fromCorydalis govanianaWall. Roots on Human Breast Cancer (MCF-7) Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Nivethika Sivakumaran ◽  
Sameera R. Samarakoon ◽  
Achyut Adhikari ◽  
Meran K. Ediriweera ◽  
Kamani H. Tennekoon ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Morphological Changes ◽  
Flow Cytometric Analysis ◽  
Pcr Analysis ◽  
Human Breast ◽  
Potent Inducer ◽  
Caspase 7 ◽  
Apoptotic Effects ◽  
Mcf 7

Current breast cancer therapies have limitations in terms of increased drug resistance resulting in short-term efficacy, thus demanding the discovery of new therapeutic agents. In this study, cytotoxic activity and apoptotic effects of govaniadine isolated fromCorydalis govanianaWall. roots were determined on human breast cancer (MCF-7) cells. The SRB assay result revealed that govaniadine led to dose- and time-dependent cytotoxic effect in MCF-7 cells along with less cytotoxicity against MCF-10A cells. Govaniadine-induced apoptosis was also accompanied by upregulation ofBax,p53, andSurvivinmRNA expression as assessed by real time PCR analysis. Flow cytometric analysis with Annexin V and PI staining indicated that govaniadine is a potent inducer of apoptosis in MCF-7 cell lines. Distinctive morphological changes contributed to apoptosis and DNA laddering were observed in govaniadine-treated MCF-7 cells. Caspase-7 was significantly activated in treated MCF-7 cells. Govaniadine-treated MCF-7 cells also showed enhanced levels of intracellular reactive oxygen species (ROS) and glutathione S-transferase (GST) and decreased levels of glutathione (GSH). The results indicate that govaniadine has potent and selective cytotoxic effects against MCF-7 cells and the potential to induce caspase 7 dependent apoptosis in MCF-7 cells by activation of pathways that lead to oxidative stress.

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