A comparative study of qualitative immunochemical screening assays for the combined measurement of T-2/HT-2 in cereals and cereal-based products

2011 ◽  
Vol 4 (4) ◽  
pp. 385-394 ◽  
Author(s):  
J. Meneely ◽  
F. Ricci ◽  
S. Vesco ◽  
M. Abouzied ◽  
M. Sulyok ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Low Cost ◽  
Ease Of Use ◽  
The Other ◽  
Accuracy And Precision ◽  
Cost Implications ◽  
Electrochemical Array ◽  
The Cost ◽  
Preparation Techniques ◽  
Speed Of Analysis

Many different immunochemical platforms exist for the screening of naturally occurring contaminants in food from the low cost enzyme linked immunosorbent assays (ELISA) to the expensive instruments such as optical biosensors based on the phenomenon of surface plasmon resonance (SPR). The primary aim of this study was to evaluate and compare a number of these platforms to assess their accuracy and precision when applied to naturally contaminated samples containing HT-2/T-2 mycotoxins. Other important factors considered were the speed of analysis, ease of use (sample preparation techniques and use of the equipment) and ultimately the cost implications. The three screening procedures compared included an SPR biosensor assay, a commercially available ELISA and an enzymelinked immunomagnetic electrochemical array (ELIME array). The qualitative data for all methods demonstrated very good overall agreements with each other, however on comparison with mass spectrometry confirmatory results, the ELISA and SPR assay performed slightly better than the ELIME array, exhibiting an overall agreement of 95.8% compared to 91.7%. Currently, SPR is more costly than the other two platforms and can only be used in the laboratory whereas in theory both the ELISA and ELIME array are portable and can be used in the field, but ultimately this is dependent on the sample preparation techniques employed. Sample preparative techniques varied for all methods evaluated, the ELISA was the most simple to perform followed by that of the SPR method. The ELIME array involved an additional clean-up step thereby increasing both the time and cost of analysis. Therefore in the current format, field use would not be an option for the ELIME array. In relation to speed of analysis, the ELISA outperformed the other methods.

scholarly journals Terrain changes from images acquired on opportunistic flights by SfM photogrammetry

2017 ◽  
Vol 11 (2) ◽  
pp. 827-840 ◽  
Author(s):  
Luc Girod ◽  
Christopher Nuth ◽  
Andreas Kääb ◽  
Bernd Etzelmüller ◽  
Jack Kohler
Keyword(s):  
Low Cost ◽  
Rapid Change ◽  
Satellite System ◽  
Light Transport ◽  
Area Of Interest ◽  
Accuracy And Precision ◽  
Global Navigation Satellite ◽  
The Cost ◽  
Gnss Data

Abstract. Acquiring data to analyse change in topography is often a costly endeavour requiring either extensive, potentially risky, fieldwork and/or expensive equipment or commercial data. Bringing the cost down while keeping the precision and accuracy has been a focus in geoscience in recent years. Structure from motion (SfM) photogrammetric techniques are emerging as powerful tools for surveying, with modern algorithm and large computing power allowing for the production of accurate and detailed data from low-cost, informal surveys. The high spatial and temporal resolution permits the monitoring of geomorphological features undergoing relatively rapid change, such as glaciers, moraines, or landslides. We present a method that takes advantage of light-transport flights conducting other missions to opportunistically collect imagery for geomorphological analysis. We test and validate an approach in which we attach a consumer-grade camera and a simple code-based Global Navigation Satellite System (GNSS) receiver to a helicopter to collect data when the flight path covers an area of interest. Our method is based and builds upon Welty et al. (2013), showing the ability to link GNSS data to images without a complex physical or electronic link, even with imprecise camera clocks and irregular time lapses. As a proof of concept, we conducted two test surveys, in September 2014 and 2015, over the glacier Midtre Lovénbreen and its forefield, in northwestern Svalbard. We were able to derive elevation change estimates comparable to in situ mass balance stake measurements. The accuracy and precision of our DEMs allow detection and analysis of a number of processes in the proglacial area, including the presence of thermokarst and the evolution of water channels.

scholarly journals An IoT Smart Broiler Farming Model for Low Income Farmers

2018 ◽  
Vol 6 (3) ◽  
pp. 95
Author(s):  
Hazael Phiri ◽  
Douglas Kunda ◽  
Jackson Phiri
Keyword(s):  
Low Income ◽  
Cost Model ◽  
Low Cost ◽  
Ease Of Use ◽  
Climate Control ◽  
Proposed Model ◽  
Zigbee Protocol ◽  
Gsm Network ◽  
The Cost ◽  
Broiler House

<p class="Abstract"><span lang="EN-US">The coming of Internet of things (IoT) brings opportunities for the deploying of wireless sensor networks. One area of deployment is smart poultry farming to improve the quality and security of chicken varieties that include broilers. The quality of broilers produced is dependent on the environment in which the broilers are kept. In addition, the revenue of the farmer is guaranteed if theft of stock is prevented. The current methods farmers use are labour intensive and time consuming as they are manual. Leveraging the features of IoT and sensors can help to monitor the environment and ensure adverse conditions are reported for farmers to take action before they harm the livestock. Incorporating intruder detection when monitoring conditions in the environment can also prevent stock theft and that can increase the income obtained by farmers. For such a system to be widely adopted by low income farmers, the cost should be low compared commercially available climate control systems that are meant for commercial farmers. The system should also provide ease of use for less technically skilled farmers, reduce the time taken by farmers to take action in controlling theft and conditions in the environment and be accessible from any location other than the broiler house. In this paper, we propose a low-cost model that can be used to monitor conditions in the environment of a broiler house and send the values to the farmer in real-time. The proposed model is based on open source microcontrollers, ZigBee protocol, GSM network, mobile applications and cloud computing. </span></p>

scholarly journals ASSESSING THE ACCURACY AND PRECISION OF IMPERFECT POINT CLOUDS FOR 3D INDOOR MAPPING AND MODELING

2018 ◽  
Vol IV-4/W6 ◽  
pp. 3-10
Author(s):  
J. Chen ◽  
O. E. Mora ◽  
K. C. Clarke
Keyword(s):  
Low Cost ◽  
Random Noise ◽  
Three Dimensional ◽  
Laser Scanner ◽  
Point Clouds ◽  
Accuracy And Precision ◽  
Wide Range ◽  
Sharp Edges ◽  
The Cost ◽  
Indoor Spaces

<p><strong>Abstract.</strong> In recent years, growing public interest in three-dimensional technology has led to the emergence of affordable platforms that can capture 3D scenes for use in a wide range of consumer applications. These platforms are often widely available, inexpensive, and can potentially find dual use in taking measurements of indoor spaces for creating indoor maps. Their affordability, however, usually comes at the cost of reduced accuracy and precision, which becomes more apparent when these instruments are pushed to their limits to scan an entire room. The point cloud measurements they produce often exhibit systematic drift and random noise that can make performing comparisons with accurate data difficult, akin to trying to compare a fuzzy trapezoid to a perfect square with sharp edges. This paper outlines a process for assessing the accuracy and precision of these imperfect point clouds in the context of indoor mapping by integrating techniques such as the extended Gaussian image, iterative closest point registration, and histogram thresholding. A case study is provided at the end to demonstrate use of this process for evaluating the performance of the Scanse Sweep 3D, an ultra-low cost panoramic laser scanner.</p>

scholarly journals Ultra Low-cost DNA Miniprep Spin-Columns for Rapid Filter-Trapped Proteomics Sample Preparation

2021 ◽  
Author(s):  
Campbell Mousseau ◽  
Camille Pierre ◽  
Matthew Champion
Keyword(s):  
Sample Preparation ◽  
Proteomic Analysis ◽  
Low Cost ◽  
Dna Purification ◽  
Ionic Interactions ◽  
Spectrometric Analysis ◽  
Label Free ◽  
Proteolytic Digestion ◽  
Spin Filters ◽  
The Cost

Complete proteolytic digestion in the preparation of proteins for bottom-up proteomic analysis is substantially improved by the use of detergents for complete denaturation. This however is incompatible with many proteases, and highly detrimental to LC-MS/MS data collection. Recently, filter-based methods such as FASP (Filter-Aided Sample Prep) have seen wide use due to their ability to remove detergents and other harmful reagents prior to digestion and mass spectrometric analysis. Unfortunately, these techniques can be variable and time consuming. Suspension trapping (S-Trapping) is a newer method that utilizes a depth-filter to trap flocculated proteins, and has proven to be a faster approach for proteomic analysis. Sample preparation by these methods requires careful control of protein concentrations in order to flocculate the sample for collection, and the cost of commercial solutions can be high. We hypothesized that protein suspensions also retain on silica-based filters due to ionic interactions mediated by the presence of sodium (Na+), SO42- and PO43-. As such, we sought to investigate if very low-cost DNA purification spin-filters, so called ‘minipreps’ could efficiently and reproducibly trap proteins for digest and LC-MS/MS analysis. Using model proteins and whole-cell lysates we compared digestion efficiencies, capacities, recovery and identification rates from samples prepared using DNA-minipreps and FASP-based protocols. Samples were analyzed using nano uHPLC MS-MS/MS and Label-Free-Quantitative (LFQ) proteomics. DNA-filters show low variability, excellent recovery, sensitivity, and proteome depth from a commercially obtainable device which costs < $0.25 (US) per sample.

Septoplasty as a day-case procedure – a two centre study

1996 ◽  
Vol 110 (2) ◽  
pp. 129-131 ◽  
Author(s):  
R. Benson-Mitchell ◽  
G. Kenyon ◽  
D. Gatland
Keyword(s):  
General Hospital ◽  
Complication Rate ◽  
Selection Criteria ◽  
District General Hospital ◽  
The Other ◽  
Day Case ◽  
Septal Surgery ◽  
Cost Implications ◽  
The Cost ◽  
Strict Selection

AbstractDay-stay surgery is an integral part of Otolaryngology. Many procedures which have traditionally been thought to require overnight stay can be carried out on a day care basis. We report our experience of treating 163 patients admitted for septal surgery as a day-case procedure. The paper summarizes the experience of two centres. One of these is a London Teaching Hospital, where surgery was performed through a dedicated day-case unit, and the other is a District General Hospital where patients were admitted to a day-case unit but had their surgery on a routine in-patient list.As the result of this study we conclude that day-stay septoplasty is associated with a low complication rate and is a safe and acceptable procedure provided that strict selection criteria are followed. The cost implications are discussed.

scholarly journals A Low-Cost, Accurate, and High-Precision Fluid Dispensing System for Microscale Application

2016 ◽  
Vol 22 (2) ◽  
pp. 144-152 ◽  
Author(s):  
Champak Das ◽  
Guochun Wang ◽  
Chien Nguyen
Keyword(s):  
Low Cost ◽  
Flow Characteristics ◽  
Positive Displacement ◽  
Real Time Measurement ◽  
Fluid Dispensing ◽  
Accuracy And Precision ◽  
Displacement Pump ◽  
The Individual ◽  
Similar Accuracy ◽  
The Cost

We present here the development of a low-cost, accurate, and precise fluid dispensing system. It can be used with peristaltic or any other pump to improve the flow characteristics. The dispensing system has a range of 1 to 100 µL with accuracy of ~99.5% and standard deviation at ~150 nL over the entire range. The system developed does not depend on the accuracy or precision of the driving pump; therefore, any positive displacement pump can be used to get similar accuracy and precision, which gives an opportunity to reduce the cost of the system. The dispensing system does not require periodic calibration and can also be miniaturized for microfluidic application. Although primarily designed for aqueous liquid, it can be extended for different nonconductive liquids as well with modifications. The unit is further used for near real-time measurement of lactate from microdialysate. The individual components can easily be made disposable or sterilized for use in biomedical applications.

scholarly journals HYDRA: Introducing a Low-Cost Framework for STEM Education Using Open Tools

Electronics ◽  
2021 ◽  
Vol 10 (24) ◽  
pp. 3056
Author(s):  
Georgios Tsalmpouris ◽  
George Tsinarakis ◽  
Nikolaos Gertsakis ◽  
Savvas A. Chatzichristofis ◽  
Lefteris Doitsidis
Keyword(s):  
Stem Education ◽  
Low Cost ◽  
Low Complexity ◽  
Ease Of Use ◽  
Modular System ◽  
Sensors And Actuators ◽  
Embedded Devices ◽  
Self Confidence ◽  
Educational Framework ◽  
The Cost

STEM education is of paramount importance, especially in the lower levels of education, and it has been proven beneficial for students in many ways. Although there are various tools available, there are significant drawbacks mainly related to the cost and the ease of use. In this study, we introduce a new low-cost educational framework oriented toward elementary and secondary educational needs. The proposed system exploits open tools and low-cost devices. The system’s core is based on the popular Arduino microcontroller, a low-cost device supported by a large community. The overall system was designed and developed, providing an expandable, modular system of low complexity suitable for students with no or low prior knowledge in related subjects, among others, to programming, embedded devices, sensors and actuators, as well as robotics. Our scope was to provide a system with a small learning curve. Practically, this makes it possible in a short amount of time for the students to perform appealing yet straightforward tasks which will boost their self-confidence and creativity, improve their technical skills and simultaneously provide a system with several capabilities usable in different kinds of projects. The introduced system was tested through a preliminary study using flow theory in a team of 68 students of the three last grades in an elementary school in Greece.

Access Grid

2008 ◽  
pp. 256-282
Author(s):  
Richard Caladine
Keyword(s):  
Distance Education ◽  
Teaching And Learning ◽  
Instant Messaging ◽  
Low Cost ◽  
Ease Of Use ◽  
The Other ◽  
Video Communications ◽  
Video Chat ◽  
E Learning ◽  
Access Grid

Videoconference has been used for interactions between students and teachers in distance education for many years. The newer technology, video chat, a computerbased technology that has evolved from instant messaging, appears to be displacing videoconference due to its low cost and ease of use. Access Grid is a similar technology in that like the other two it is based on two-way audio and video communications. Since its inception in the mid-1990s, the Access Grid has been used by researchers and it is not surprising that usage levels in teaching and learning are increasing as many researchers are teachers and see its potential to bring media-rich interactions to e-learning.

scholarly journals Recent Techniques in Nutrient Analysis for Food Composition Database

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4567
Author(s):  
Mohd Fairulnizal Md Noh ◽  
Rathi Devi-Nair Gunasegavan ◽  
Norhayati Mustafa Khalid ◽  
Vimala Balasubramaniam ◽  
Suraiami Mustar ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Method Development ◽  
Low Cost ◽  
Food Composition ◽  
Nutritional Composition ◽  
Ease Of Use ◽  
Health Food ◽  
Nutrient Analysis ◽  
Nutrition Labelling ◽  
Wide Range

Food composition database (FCD) provides the nutritional composition of foods. Reliable and up-to date FCD is important in many aspects of nutrition, dietetics, health, food science, biodiversity, plant breeding, food industry, trade and food regulation. FCD has been used extensively in nutrition labelling, nutritional analysis, research, regulation, national food and nutrition policy. The choice of method for the analysis of samples for FCD often depends on detection capability, along with ease of use, speed of analysis and low cost. Sample preparation is the most critical stage in analytical method development. Samples can be prepared using numerous techniques; however it should be applicable for a wide range of analytes and sample matrices. There are quite a number of significant improvements on sample preparation techniques in various food matrices for specific analytes highlighted in the literatures. Improvements on the technology used for the analysis of samples by specific instrumentation could provide an alternative to the analyst to choose for their laboratory requirement. This review provides the reader with an overview of recent techniques that can be used for sample preparation and instrumentation for food analysis which can provide wide options to the analysts in providing data to their FCD.

scholarly journals Design of a Low Cost Switching Board Enabling a Reconfigurable Remote Experiment

2019 ◽  
Vol 15 (12) ◽  
pp. 32
Author(s):  
Abderrahmane Adda Benattia ◽  
Mohamed Moussa ◽  
Abdelhalim Benachenhou ◽  
Abdelhamid Mebrouka
Keyword(s):  
Low Cost ◽  
Ease Of Use ◽  
Practical Work ◽  
Circuit Board ◽  
Raspberry Pi ◽  
Measurement Instruments ◽  
Hardware Platform ◽  
Hardware Configuration ◽  
Spi Bus ◽  
The Cost

<p class="0abstract">Most of currently remote laboratories implementations include interactive experimentation. In this case, students use real devices and equipment to perform real experiments, which need some flexibility of interaction with the hardware platform. The hardware platform is composed of a Raspberry Pi as a lab server, a switching board (SB), a practical work circuit board and some measurement instruments. The SB is used to make configuration of experimentation by establishing connection between the practical work circuit and measurement instruments. During the experimentation process, students change the setup using a web page. In the background, the hardware configuration is realized using SB, which is controlled by the lab server. The purpose of this work is to develop a new SB in order to provide more possibilities, interaction flexibility with the hardware platform, ease of use, improve performance in response time and finally reduce the cost of the hardware. The SB is based on switches instead of relays. This board can be plugged directly on a Raspberry Pi to facilitate the assembly. It extends the “SPI” bus in order to control some electronic components such as digital potentiometers. Its use is illustrated with a circuit with multiple combinations.</p>

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