Comparative Assessment of RAPD and ISSR Markers to Study Genetic Polymorphism in Colletotrichum gloeosorioides Isolates of Mango

Objective: Total investigation of the macro-and micromorphological features of Citrus maxima (Burm.) Merrill. In addition, DNA fingerprinting in this study was to estimate genetic polymorphism and relationships with another citrus viz: Citrus paradise and Citrus sinensis based on RAPD and ISSR markers.Methods: For the establishment of macro-and micromorphological investigation and genetic estimation, this study presents a total investigation of the macro-and micromorphological features of the roots, stems, leaves, flowers, fruits and seeds of the plants through microscopical investigation of the prepared entire, transverse sections and powdered forms of different organs under study. Furthermore, the DNA of the three plants was extracted from leaf samples and two types of DNA markers RAPD and ISSR were assayed.Results: Macro-and micromorphological features of different organs were investigated. On the other hand Genetic polymorphism and relationships among Citrus maxima, Citrus paradisi and Citrus sinensis was revealed. The highest genetic similarity was between Citrus maxima and Citrus paradisi while the lowest similarity was between Citrus paradisi and Citrus sinensis as revealed by RAPD, ISSR and combined data.Conclusion: For the present study, macro and micro-morphological characters, as well as, DNA fingerprinting can be considered as the identifying parameters for authentication of Citrus maxima (Burm.) Merrill.

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Comparative assessment of genetic diversity among Indian bamboo genotypes using RAPD and ISSR markers

Molecular Biology Reports ◽

10.1007/s11033-015-3867-9 ◽

2015 ◽

Vol 42 (8) ◽

pp. 1265-1273 ◽

Cited By ~ 12

Author(s):

Parth Desai ◽

Bhavesh Gajera ◽

Mounil Mankad ◽

Shikha Shah ◽

Armi Patel ◽

...

Keyword(s):

Genetic Diversity ◽

Issr Markers ◽

Comparative Assessment ◽

Rapd And Issr

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Genetic polymorphism evaluation of spring triticale (×Triticosecale wittmack) samples with use of RAPD and ISSR markers

Russian Journal of Genetics Applied Research ◽

10.1134/s207905971206010x ◽

2012 ◽

Vol 2 (6) ◽

pp. 508-512 ◽

Cited By ~ 1

Author(s):

O. A. Orlovskaya ◽

L. V. Koren ◽

L. V. Khotyleva

Keyword(s):

Genetic Polymorphism ◽

Issr Markers ◽

Spring Triticale ◽

Triticosecale Wittmack ◽

Rapd And Issr

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Correction to: Efficient in vitro plant regeneration from leaf-derived callus and genetic fidelity assessment of an endemic medicinal plant Ranunculus wallichianus Wight & Arnn by using RAPD and ISSR markers

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-021-02153-9 ◽

2021 ◽

Author(s):

P. Srinivasan ◽

H. David Raja ◽

R. Tamilvanan

Keyword(s):

Plant Regeneration ◽

Medicinal Plant ◽

Genetic Fidelity ◽

Issr Markers ◽

In Vitro Plant Regeneration ◽

Fidelity Assessment ◽

In Vitro Plant ◽

Endemic Medicinal Plant ◽

Rapd And Issr

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Collection and characterization of banana gene pools (Musa spp.) in Manipur (N.E. India) using PCR–RFLP and RAPD and ISSR markers

Revista Brasileira de Botânica ◽

10.1007/s40415-021-00722-y ◽

2021 ◽

Author(s):

Warepam Amuchou Singh ◽

Nandeibam Samarjit Singh ◽

Elangbam Julia Devi ◽

Pratap Jyoti Handique ◽

Huidrom Sunitibala Devi

Keyword(s):

Issr Markers ◽

Gene Pools ◽

Musa Spp ◽

Pcr Rflp ◽

Rapd And Issr

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Species-diagnostic and species-specific DNA sequences evenly distributed throughout pine and spruce chromosomes

Genome ◽

10.1139/g10-065 ◽

2010 ◽

Vol 53 (10) ◽

pp. 769-777 ◽

Cited By ~ 1

Author(s):

Melanie Mehes-Smith ◽

Paul Michael ◽

Kabwe Nkongolo

Keyword(s):

Dna Sequences ◽

Random Amplified Polymorphic Dna ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Pinus Monticola ◽

The Family ◽

Species Specific ◽

Rapd And Issr ◽

Specific Sequences

Genome organization in the family Pinaceae is complex and largely unknown. The main purpose of the present study was to develop and physically map species-diagnostic and species-specific molecular markers in pine and spruce. Five RAPD (random amplified polymorphic DNA) and one ISSR (inter-simple sequence repeat) species-diagnostic or species-specific markers for Picea mariana , Picea rubens , Pinus strobus , or Pinus monticola were identified, cloned, and sequenced. In situ hybridization of these sequences to spruce and pine chromosomes showed the sequences to be present in high copy number and evenly distributed throughout the genome. The analysis of centromeric and telomeric regions revealed the absence of significant clustering of species-diagnostic and species-specific sequences in all the chromosomes of the four species studied. Both RAPD and ISSR markers showed similar patterns.

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Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-019-0011-6 ◽

2019 ◽

Vol 17 (1) ◽

Cited By ~ 1

Author(s):

Marwa Hamouda

Keyword(s):

Genetic Diversity ◽

Electrophoretic Pattern ◽

Issr Markers ◽

Silybum Marianum ◽

Inter Simple Sequence Repeats ◽

Sds Page ◽

Dna Pattern ◽

Pharmaceutical Properties ◽

High Level ◽

Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

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