Chemical compositon, antibacterial and antioxidant activities of Cnidium silaifolium ssp. orientale (Boiss.) Tutin essential oils

The chemical compositions of the essential oils (EOs) obtained by hydrodistillation from different parts of Cnidium silaifolium ssp. orientale (Boiss.) Tutin were analyzed both by GC-FID and GC/MS, simultaneously. One hundred nine compounds representing 90.1% of the total volatiles in the EOs were identified with the main characteristic compounds α-pinene (50.3%) in the root, germacrene D (20.3%) in the fruit, and β-caryophyllene (18.7%) in the aerial parts of C. silaifolium ssp. orientale. The antimicrobial activity against human pathogenic Gram-negative and Gram-positive bacteria was evaluated by the in vitro microdilution method. Antibacterial succeptibility was observed from the root and aerial part EOs against Staphylococcus aureus (0.039 and 0.156 mg/mL, respectively); while the fruit EO was most effective against Bacillus cereus at 0.07 mg/mL. The antioxidant capacities of the EOs were also evaluated by in vitro DPPH• and ABTS•+ scavenging assays, where no significant activity was observed compared to ascorbic acid and Trolox.

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Chemical Compositions and Antimicrobial Activity of the Essential Oils of Piper abbreviatum, P. erecticaule and P. lanatum (Piperaceae)

Natural Product Communications ◽

10.1177/1934578x1400901235 ◽

2014 ◽

Vol 9 (12) ◽

pp. 1934578X1400901 ◽

Cited By ~ 1

Author(s):

Wan Mohd Nuzul Hakimi Wan Salleh ◽

Farediah Ahmad ◽

Khong Heng Yen

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Antimicrobial Agents ◽

Antimicrobial Activities ◽

Dilution Method ◽

Chemical Compositions ◽

Gram Positive Bacteria ◽

Aerial Parts ◽

Ms Analysis ◽

Weak Activity

The study was designed to examine the chemical composition and antimicrobial activities of essential oils extracted from the aerial parts of three Piper species: Piper abbreviatum, P. erecticaule and P. lanatum, all from Malaysia. GC and GC/MS analysis showed qualitative and quantitative differences between these oils. GC and GC-MS analysis of P. abbreviatum, P. erecticaule and P. lanatum oils resulted in the identification of 33, 35 and 39 components, representing 70.5%, 63.4% and 78.2% of the components, respectively. The major components of P. abbreviatum oil were spathulenol (11.2%), ( E)-nerolidol (8.5%) and β-caryophyllene (7.8%), whereas P. erecticaule oil mainly contained β-caryophyllene (5.7%) and spathulenol (5.1%). Borneol (7.5%), β-caryophyllene (6.6%) and α-amorphene (5.6%) were the most abundant components in P. lanatum oil. Antimicrobial activity was carried out using disc diffusion and broth micro-dilution method against nine microorganisms. All of the essential oils displayed weak activity towards Gram-positive bacteria with MIC values in the range 250–500 μg/mL. P. erecticaule oil showed the best activity on Aspergillus niger (MIC 31.3 μg/mL), followed by P. lanatum oil (MIC 62.5 μg/mL). This study demonstrated that the essential oils have potential as antimicrobial agents and may be useful in the pharmaceutical and cosmetics industries.

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Chemical Composition and Antimicrobial Activity of Teucrium Capitatum L. Subsp. Lusitanicum (Schreb.) T. Navarro & Rosua Essential Oil

Journal of Chromatographic Science ◽

10.1093/chromsci/bmaa086 ◽

2020 ◽

Author(s):

Haïfa Debbabi ◽

Ridha El Mokni ◽

Ibrahim Jlassi ◽

Rajesh K Joshi ◽

Saoussen Hammami

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Properties ◽

Significant Activity ◽

Bacterial Strains ◽

Gram Positive Bacteria ◽

Sesquiterpene Hydrocarbons ◽

Germacrene D

Abstract Chemical composition and antimicrobial activity of Teucrium capitatum L. subsp. lusitanicum essential oil was investigated for the first time in the present study. Qualitative and quantitative analyses of the chemical composition by gas chromatography and mass spectrometry (GC–FID and GC–MS) revealed the presence of 60 compounds representing 97.6% of the whole constituents. The main compounds were germacrene D (47.1%), spathulenol (5.8%), α-selinene (5.3%), germacrene A (2.9%), δ-cadinene (2.8%) and cubenol (2.7%). In vitro, the antimicrobial activity was investigated against five bacterial strains along with the yeast Candida albicans using broth microdilution assay. T. capitatum subsp. lusitanicum essential oil showed significant activity against the gram-positive bacteria Staphylococcus aureus (MIC = MBC = 78 μg mL−1), Bacillus subtilis (MIC = MBC = 156 μg mL−1) and the yeast C. albicans (MIC = MFC = 156 μg mL−1). The great potential of antimicrobial effects is most likely due to the very high percentage of sesquiterpene hydrocarbons particularly to germacrene D, for which the antimicrobial properties have been previously reported.

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The chemical composition, antimicrobial, and antioxidant activities of Pycnocycla spinosa and Pycnocyla flabellifolia essential oils

Zeitschrift für Naturforschung C ◽

10.1515/znc-2016-0038 ◽

2016 ◽

Vol 71 (11-12) ◽

pp. 403-408 ◽

Cited By ~ 2

Author(s):

Mohaddese Mahboubi ◽

Elaheh Mahdizadeh ◽

Rezvan Heidary Tabar

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Inhibition Zone ◽

Chemical Compositions ◽

Caryophyllene Oxide ◽

Aerial Parts ◽

Dilution Assay ◽

Main Components ◽

Broth Dilution

Abstract The purpose of our study was to compare the chemical compositions and antimicrobial and antioxidant activities of Pycnocycla spinosa and Pycnocycla flabellifolia essential oils. cis-Asarone (62.5%) and widdra-2,4(14)-diene (9%) were the main components of P. spinosa aerial part essential oil, while elemicin (60.1%) and caryophyllene oxide (9.8%) were the main components of P. spinosa seed essential oil. α-Phellandrene (25.5%), p-cymene (15.3%), and limonene (13.3%) were found in P. flabellifolia essential oil. The inhibition zone diameters for P. flabellifolia essential oil were significantly higher than for the two other essential oils from P. spinosa (p<0.05). In broth dilution assay (µL/mL), the sensitive microorganism to Pycnocycla sp. (P. spinosa, P. flabellifolia) was Aspergillus niger, followed by Candida albicans. In 2,2-diphenyl-1-picrylhydrazyl (DPPH) system, P. spinosa aerial parts essential oil (IC50=548 µg/mL) had higher antioxidant activity than that of two other essential oils.

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Chemical Composition and Anti-mildew Activities of Essential Oils from Different Parts of Michelia compressa var. formosana

Natural Product Communications ◽

10.1177/1934578x1501000435 ◽

2015 ◽

Vol 10 (4) ◽

pp. 1934578X1501000

Author(s):

Yu-Chang Su ◽

Kuang-Ping Hsu ◽

Eugene I-Chen Wang ◽

Chen-Lung Ho

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Main Constituent ◽

Germacrene D ◽

Leaf Oil ◽

Essential Oil Yield ◽

Different Parts ◽

Type Apparatus

In this study, anti-mildew activities of the essential oils of the leaves, wood, twigs, flowers, and their constituents from Michelia compressa var. formosana were evaluated in vitro against 7 mildew fungi. The main compounds responsible for the anti-mildew activities were isolated and identified. The essential oils from the fresh leaves, wood, twigs, and flowers of M. compressa var. formosana were isolated using hydrodistillation in a Clevenger-type apparatus, and characterized by GC-FID and GC-MS. The essential oil yield from the flowers was the highest, and the oils obtained from the 4 parts of the tree were composed mainly of sesquiterpenoids. The leaf oil consisted primarily of α-cadinol (18.9%) and germacrene D (18.5%); the twig oil's main constituent was α-cadinol (19.1%); the wood oil's major components were α-cadinol (25.7%) and β-eudesmol (20.2%); and the flower oil contained α-cadinol (11.4%). Comparing the anti-mildew activities of the oils suggested that wood oil was the most effective. Further fractionation of the wood oil produced α-cadinol, β-eudesmol, τ-cadinol, and elemol. The 4 compounds exhibit very strong anti-mildew activities, and these in descending order are α-cadinol, elemol, β-eudesmol, and τ-cadinol. For the anti-mildew activities of the wood oil, the active source compounds were determined to be α-cadinol, elemol, β-eudesmol, and τ-cadinol.

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Assessment of the antibacterial activity of four essential oils and the biobactericide Neco.

International Journal of Phytomedicine ◽

10.5138/09750185.2106 ◽

2017 ◽

Vol 9 (3) ◽

pp. 443 ◽

Cited By ~ 1

Author(s):

Jean-Fabrice Yala ◽

Rolande Mabika Mabika ◽

Brahima Camara ◽

Seydou Tuo ◽

Alain Souza ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oils ◽

Diffusion Method ◽

Melaleuca Quinquenervia ◽

Eucalyptus Citriodora ◽

Cymbopogon Citratus ◽

Gram Positive Bacteria ◽

Lippia Multiflora ◽

Microdilution Method

The purpose of this study was to assess in vitro the activity of four essential oils (Cymbopogon citratus, Eucalyptus citriodora, Lippia multiflora, Melaleuca quinquenervia) and the biobactericide Neco® on Gram-positive bacteria.The aromatogram and antibiogram were assessed by the agar well diffusion method and the Muller Hinton disk-agar diffusion method, respectively. Also, the minimum inhibitory concentration and the minimum bactericidal concentration were determined by the microdilution method in liquid medium.The aromatogram showed that the biobactericide Neco® induced the largest inhibition diameters (34.53 ± 11.82 - 43.92 ± 5.38 mm) of all strains combined, followed by the essential oils of Eucalyptus Citriodora (30.01 ± 3.02 - 41.89 ± 1.77 mm) and Lippia multiflora (20.72 ± 4.72 - 37.61 ± 2.80 mm). However, the essential oils of Melaleuca quinquenervia (19.99 ± 3.93 - 26.20 ± 13.27 mm) and Cymbopogon citratus (13.52 ± 3.59 - 29.08 ± 2.35 mm) had the smallest inhibition diameters. Moreover, the comparison of the activities of the aromatogram and antibiogram revealed generally that activities were higher with essential oils than with antibiotics.At the end of this study, the essential oils of Cymbopogon citratus, Eucalyptus citriodora, Lippia multiflora, Melaleuca quinquenervia and the biobactericide Neco® had an antibacterial activity on Gram+ bacteria.

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In vitro Antiprotozoal Activity and Chemical Composition of Ambrosia tenuifolia and A. scabra Essential Oils

Natural Product Communications ◽

10.1177/1934578x0800300416 ◽

2008 ◽

Vol 3 (4) ◽

pp. 1934578X0800300 ◽

Cited By ~ 2

Author(s):

Valeria P. Sülsen ◽

Silvia I. Cazorla ◽

Fernanda M. Frank ◽

Paola M. R. Di Leo Lira ◽

Claudia A. Anesini ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Inhibitory Concentration ◽

Antimalarial Activity ◽

Caryophyllene Oxide ◽

Antiprotozoal Activity ◽

Aerial Parts ◽

Germacrene D ◽

First Time

The in vitro antimalarial, trypanocidal and leishmanicidal activities and the chemical composition of Ambrosia tenuifolia and A. scabra essential oils were investigated. The oils, obtained by hydrodistillation of the aerial parts, showed significant antimalarial activity against the chloroquine sensitive (F32) and the chloroquine resistant (W2) strains of Plasmodium falciparum with 50% inhibitory concentration (IC50) values ranged from 2.8 to 7 μg/mL. Against Trypanosoma cruzi epimastigotes, only A. tenuifolia exerted slight activity (IC50 = 59.7 μg/mL). None of the essential oils showed toxicity towards Leishmania mexicana. Cytotoxicity for murine T-lymphocytes was compared with the parasiticidal activity by using the selectivity index (SI) ratio. The chemical composition of A. tenuifolia and A. scabra essential oils was analyzed by GC-FID-MS. Thirty-eight and twenty-four compounds were identified in A. tenuifolia and A. scabra, respectively. The major constituents of A. tenuifolia oil were germacrene D (22.9%) and bicyclogermacrene (14.2%), while the oil of A. scabra contained limonene (22.0%), caryophyllene oxide (13.8%) and ( E)-caryophyllene (13.5%). This is the first time that the antiprotozoal activity of both oils and the chemical composition of A. scabra essential oil have been reported.

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Chemical Compositions and Antimicrobial Activity of the Essential Oils of Hornstedtia havilandii (Zingiberaceae)

Natural Product Communications ◽

10.1177/1934578x1400900133 ◽

2014 ◽

Vol 9 (1) ◽

pp. 1934578X1400900 ◽

Cited By ~ 4

Author(s):

Siti Ernieyanti Hashim ◽

Hasnah Mohd Sirat ◽

Khong Heng Yen

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Chemical Compositions ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Germacrene D ◽

Weak Activity ◽

Main Components

The chemical compositions and antimicrobial activity of the fresh rhizome and flower oils of Hornstedtia havilandii were studied. The components present were analyzed using GC and GC-MS. A total of forty-eight constituents were successfully identified from the flower and rhizome oils, representing 93.4% and 89.6%, respectively. The most abundant components of the flower oil were β-pinene, (19.5%), β-elemene, (10.0%), β-cubebene, (6.2%), α-pinene, (5.6%), γ-cadinene, (4.3%) and germacrene D, (3.3%), while α-copaene, (10.2%), β-selinene, (8.4%), β-elemene, (7.0%), γ-cadinene, (6.9%), β-cubebene, (5.3%) and germacrene D, (5.3%) were found as the main components of the rhizome oil. The essential oils were tested for antimicrobial activity against Gram-positive bacteria, Gram-negative bacteria and yeasts. Both oils showed activity against the Gram-positive bacterium, Staphylococcus aureus, with moderate MIC values of 112.5 μg/mL, however, for Gram-negative bacteria, the flower oil showed weak antimicrobial activity with a MIC value of 225 μg/mL. Only very weak activity was shown against the yeasts Candida albicans and C. glabrata, with MIC values of 900–1800 μg/mL.

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Chemical Composition of the Essential Oils of Some Central Asian Nepeta Species (Lamiaceae) by GLC-MS

Natural Product Communications ◽

10.1177/1934578x1601101229 ◽

2016 ◽

Vol 11 (12) ◽

pp. 1934578X1601101

Author(s):

Nilufar Z. Mamadalieva ◽

Farukh S. Sharopov ◽

Prabodh Satyal ◽

Shahnoz S. Azimova ◽

Michael Wink

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Aerial Parts ◽

Central Asian ◽

Germacrene D

The chemical composition of the essential oils obtained from aerial parts of Nepeta alatavica Lipsky, N. nuda L., and N. olgae Regel (Lamiaceae), growing wild in Central Asia (Tajikistan and Uzbekistan), were analyzed by GLC and GLC-MS. The main constituents of the essential oil from N. nuda were 1,8-cineole (24.6%), 4a-α,7-β,7a-α-nepetalactone (21.0%), germacrene D (13.5%), and β-caryophyllene (12.7%), whereas those of N. alatavica were thymol (48.5%), verbenone (7.7%), and carvacrol (7.5%). In the essential oil of N. olgae the most abundant compounds were acetylcyclohexene (31.5%), 4-tridecyne (13.2%), 2-methyl cyclopentanone (6.8%) and 1,8-cineole (6.0%). The potential antioxidant activity of these essential oils was studied by using DPPH, ABTS and FRAP assays. The essential oil of N. alatavica showed the highest in vitro free radical scavenging activity, probably due to its high thymol and carvacrol content.

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Anacyclus pyrethrum var. pyrethrum (L.) and Anacyclus pyrethrum var. depressus (Ball) Maire: Correlation between Total Phenolic and Flavonoid Contents with Antioxidant and Antimicrobial Activities of Chemically Characterized Extracts

Plants ◽

10.3390/plants10010149 ◽

2021 ◽

Vol 10 (1) ◽

pp. 149

Author(s):

Fatima Zahra Jawhari ◽

Abdelfattah E. L. Moussaoui ◽

Mohammed Bourhia ◽

Hamada Imtara ◽

Hamza Saghrouchni ◽

...

Keyword(s):

Antioxidant Activities ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Total Phenolic ◽

Chemical Compositions ◽

Antioxidant Power ◽

Phenolic And Flavonoid ◽

Antioxidant And Antimicrobial Activities ◽

Different Parts

In this work, two varieties of Anacyclus pyrethrum (L.) including Anacyclus pyrethrum var. pyrethrum (L.) and Anacyclus pyrethrum var. depressus (Ball) Maire were evaluated for their mineral and chemical compositions, total phenolic and flavonoid contents, and antimicrobial and antioxidant activities using hydroalcoholic extracts from their different parts (leaves, capitula, roots, and seeds). The phytochemical and mineral compositions were carried out using standard methods. The antioxidant activity was determined using the DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azino-bis 3-ethylbenzothiazolin-6-sulfonic acid), and FRAP (ferric reducing antioxidant power) tests. The antimicrobial activity was assayed using the agar diffusion, minimum inhibitory concentration, and minimum bactericidal concentration methods. The results of the chemical analysis showed that both varieties contained interesting mineral and chemical compositions with potentially active compounds; among them, N-isobutyl-2,4-heptadiene-6-monoynamide and cinnamic acid were detected in the Anacyclus pyrethrum var. pyrethrum (L.) only while thiadiazolo [5,4-d] pyrimidin-7-amine and N-isobutyl-2,4-undecadiene-8,10-diynamide compounds were limited to the Anacyclus pyrethrum var. depressus (Ball) Maire. In vitro antioxidant and antimicrobial activities of the two varieties demonstrated that the different parts had prominent antioxidant and antimicrobial properties. The principal component analysis (PCA) showed great similarity in the activity of the leaves, capitula, and seeds of both plants and a high difference in roots. Anacyclus pyrethrum var. pyrethrum roots were characterized by a high content in phenols and flavonoids and better antibacterial activities compared to Anacyclus pyrethrum var. depressus (Ball) Maire roots, which were characterized by better antioxidant activities. From this study, it can be concluded that the two varieties of Anacyclus pyrethrum (L.) showed promising mineral and chemical compositions with antioxidant and antimicrobial properties.

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Constituents, Antibacterial and Antioxidant Activities of Essential Oils from Trachelospermum jasminoides Flowers

Natural Product Communications ◽

10.1177/1934578x1400901234 ◽

2014 ◽

Vol 9 (12) ◽

pp. 1934578X1400901 ◽

Cited By ~ 3

Author(s):

Acharavadee Pansanit ◽

Patcharee Pripdeevech

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antioxidant Activities ◽

Antibacterial Activities ◽

Chemical Compositions ◽

Oil Composition ◽

Chiang Mai ◽

Gram Positive Bacteria ◽

Linalool Oxide ◽

Total Oil

The present work reports the chemical compositions, antibacterial and antioxidant activities of essential oils from T. jasminoides flowers collected from two different geographical areas, Chiang Rai and Chiang Mai, Thailand. The essential oil of T. jasminoides from the Chiang Rai area had 99 compounds representing 97.9% of the total oil composition, with E-nerolidol and α-phellandrene as the major constituents. In contrast, the essential oil of T. jasminoides collected from the Chiang Mai area contained 93 components representing 94.8% of the total oil, with trans-linalool oxide and citronellol as the major compounds. Flower oils of T. jasminoides exhibited greater antibacterial activities against Gram-negative bacteria than Gram-positive bacteria. Both oils displayed antioxidant activities.

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