scholarly journals A Guide to Sexing Salamanders of The Central Appalachians, USA

Author(s):  
Lacy E Rucker ◽  
Donald J Brown ◽  
Carl D Jacobsen ◽  
Kevin R Messenger ◽  
Erik R Wild ◽  
...  

Documenting the sex of individuals encountered during wildlife research and monitoring activities is important for understanding and tracking changes in populations. However, sexing salamanders can be particularly difficult because secondary sex characters are often subtle or only visible during the breeding season, and guidance on species-specific sex determination is lacking from most field guides. The purpose of this guide is to provide a reference to assist biologists in the Central Appalachian region with identifying sex of live adult salamanders. In the main text we provide summary tables and figures to serve as concise references in the field. In Text S1 (Supplemental Material) we provide individual species accounts that contain concise yet comprehensive information for each species based on the published literature, as well as many images depicting sexually dimorphic characters. Our focal region encompasses partial or entire distributions for 56 species of salamanders in five families (Ambystomidae, Cryptobranchidae, Plethodontidae, Proteidae, and Salamandridae). We identified seven morphological characters that are strongly sexually dimorphic and useful for sexing live, non-anesthetized, adult salamanders in the field, with males of individual species exhibiting one to five of the characters. We identified >20 additional characters that are weakly sexually dimorphic, difficult to distinguish in the field, or species-specific. Our guide serves as a synthesis of sexually dimorphic characters available for salamanders in Central Appalachia, and we anticipate it will have broad value for researchers, monitoring programs, and salamander enthusiasts in eastern and central North America.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Arangasamy Yazhini ◽  
Narayanaswamy Srinivasan ◽  
Sankaran Sandhya

AbstractAfrotheria is a clade of African-origin species with striking dissimilarities in appearance and habitat. In this study, we compared whole proteome sequences of six Afrotherian species to obtain a broad viewpoint of their underlying molecular make-up, to recognize potentially unique proteomic signatures. We find that 62% of the proteomes studied here, predominantly involved in metabolism, are orthologous, while the number of homologous proteins between individual species is as high as 99.5%. Further, we find that among Afrotheria, L. africana has several orphan proteins with 112 proteins showing < 30% sequence identity with their homologues. Rigorous sequence searches and complementary approaches were employed to annotate 156 uncharacterized protein sequences and 28 species-specific proteins. For 122 proteins we predicted potential functional roles, 43 of which we associated with protein- and nucleic-acid binding roles. Further, we analysed domain content and variations in their combinations within Afrotheria and identified 141 unique functional domain architectures, highlighting proteins with potential for specialized functions. Finally, we discuss the potential relevance of highly represented protein families such as MAGE-B2, olfactory receptor and ribosomal proteins in L. africana and E. edwardii, respectively. Taken together, our study reports the first comparative study of the Afrotherian proteomes and highlights salient molecular features.


Zootaxa ◽  
2007 ◽  
Vol 1627 (1) ◽  
pp. 23-39 ◽  
Author(s):  
NATAN M. MACIEL ◽  
REUBER A. BRANDÃO ◽  
LEANDRO A. CAMPOS ◽  
ANTONIO SEBBEN

A new toad, Rhinella cerradensis, is described, including its tadpole and the advertisement call. The new species occupies Cerrado habitats in the Brazilian states of Piauí, Bahia, Goiás, Minas Gerais, and Distrito Federal. The species is characterized by its large size; absence of tibial glands; well developed cranial crests; short hands; sexually dimorphic coloration; and by the absence of a spiracle tube of the tadpole. The new species is included in the Rhinella marina group by the presence of a jagged suture formed by the articulation between the pterygoid medial ramus and the parasphenoid alae, as well as other shared morphological features. Morphological characters and statistical analyses inferred by morphometric feature suggest the existence of two subgroups of species within R. marina group. However, taxonomic rearrangements are not made here and await phylogenetic analysis.


Phytotaxa ◽  
2021 ◽  
Vol 502 (2) ◽  
pp. 191-207
Author(s):  
SHIVANI KASHYAP ◽  
CHANDAN KUMAR SAHU ◽  
ROHIT KUMAR VERMA ◽  
LAL BABU CHAUDHARY

Due to large size and enormous morphological plasticity, the taxonomy of the genus Astragalus is very complex and challenging. The identification and grouping of species chiefly based on macromorphological characters become sometimes difficult in the genus. In the present study, the micromorphology of the seeds of 30 species belonging to 14 sections of Astragalus from India has been examined applying scanning electron microscopy (SEM) along with light microscopy (LM) to evaluate their role in identification and classification. Attention was paid to colour, shape, size and surface of seeds. The overall size of the seeds ranges from 1.5–3.2 × 0.8–2.2 mm. The shape of the seeds is cordiform, deltoid, mitiform, orbicular, ovoid and reniform. The colour of seeds varies from brown to blackish-brown to black. Papillose, reticulate, ribbed, rugulate and stellate patterns were observed on the seed coat surface (spermoderm) among different species. The study reveals that the seed coat ornamentations have evolved differently among species and do not support the subgeneric and sectional divisions of the genus. However, they add an additional feature to the individual species, which may help in identification in combination with other macro-morphological features.


2010 ◽  
Vol 3 (1) ◽  
pp. 63-96 ◽  
Author(s):  
Rodrigo Pereira ◽  
Satoko Narita ◽  
Daisuke Kageyama ◽  
Finn Kjellberg

AbstractArthropods are sexually dimorphic. An arthropod individual usually differentiates into a male or a female. With very low frequencies, however, individuals with both male and female morphological characters have repeatedly been found in natural and laboratory populations of arthropods. Gynandromorphs (i.e., sexual mosaics) are genetically chimeric individuals consisting of male and female tissues. On the other hand, intersexes are genetically uniform (i.e., complete male, complete female or intermediate in every tissue) but all or some parts of their tissues have either a sexual phenotype opposite to their genetic sex or an intermediate sexual phenotype. Possible developmental processes (e.g., double fertilization of a binucleate egg, loss of a sex chromosome or upregulation/downregulation of sex-determining genes) and causal factors (e.g., mutations, genetic incompatibilities, temperatures or endosymbionts) for the generation of gynandromorphs and intersexes are reviewed and discussed.


2016 ◽  
Vol 371 (1694) ◽  
pp. 20150269 ◽  
Author(s):  
Santiago Soliveres ◽  
Peter Manning ◽  
Daniel Prati ◽  
Martin M. Gossner ◽  
Fabian Alt ◽  
...  

Species diversity promotes the delivery of multiple ecosystem functions (multifunctionality). However, the relative functional importance of rare and common species in driving the biodiversity–multifunctionality relationship remains unknown. We studied the relationship between the diversity of rare and common species (according to their local abundances and across nine different trophic groups), and multifunctionality indices derived from 14 ecosystem functions on 150 grasslands across a land-use intensity (LUI) gradient. The diversity of above- and below-ground rare species had opposite effects, with rare above-ground species being associated with high levels of multifunctionality, probably because their effects on different functions did not trade off against each other. Conversely, common species were only related to average, not high, levels of multifunctionality, and their functional effects declined with LUI. Apart from the community-level effects of diversity, we found significant positive associations between the abundance of individual species and multifunctionality in 6% of the species tested. Species-specific functional effects were best predicted by their response to LUI: species that declined in abundance with land use intensification were those associated with higher levels of multifunctionality. Our results highlight the importance of rare species for ecosystem multifunctionality and help guiding future conservation priorities.


Author(s):  
Huaijiang He ◽  
Chunyu Zhang ◽  
Fengguo Du ◽  
Xiuhai Zhao ◽  
Song Yang ◽  
...  

Understory plants are important components of forest ecosystem productivity and diversity. Compared to biomass models of overstory canopy trees, few are available for understory saplings and shrubs and therefore their roles in estimation of forest carbon pools are often ignored. In this study, we harvested 24 understory species including 4 saplings, 9 tree-like shrubs and 11 typical shrubs in coniferous and broadleaved mixed forest in northeastern China and developed the best fit allometric equations of above- and below-ground and total biomass by species-specific or multispecies using morphological measurements of basal diameter, height and crown area as independent variables. The result showed that single basal diameter, height or crown area had good explanatory power for both species-specific and multispecies (p&lt;0.001). The best-fit models included only basal diameter in sapling and tree-like shrubs, and combinations of crown area, height, and basal diameter in typical shrubs. The logarithmic model was most desired among the 4 model forms of linear, quadratic, multiple linear and logarithmic, for species-specific and multispecies. The models we developed should help the estimation of forest ecosystem carbon stocks, especially for belowground component, and provide tools for quantification of individual species biomass of understory plants.


2015 ◽  
Vol 24 (1) ◽  
pp. 34-40 ◽  
Author(s):  
B. P. Heyojoo ◽  
S. Nandy

This study aims to estimate above-ground phytomass and carbon of TROF ecosystem in part of Bijnor district in Uttar Pradesh state of India using IRS P6 LISS-IV satellite image by geo-spatial approach coupled with field sampling. Chacko’s formula was referred to compute number of samples in each TROF types and the sample plot size in each stratum was adopted from Vegetation Carbon Project (VCP) under National Carbon Project (NCP). With the help of field data consisting mainly the height and girth information, volume of each individual tree per plot was obtained using site and tree species-specific standard volumetric equations. The phytomass was calculated by multiplying volume with Biomass Expansion Factor (BEF) then with regional specific gravity of the individual species and summed up in each plot to get total phytomass per plot. The total phytomass per plot was reported to be maximum 544.00 t/ha for linear TROF followed by 121.89 t/ha for block TROF. The carbon from phytomass was obtained by multiplying the total phytomass by a conversion factor that represents the average carbon content in phytomass. Spectral modeling for phytomass with different bands and indices were established and the best fit curve (R2 = 0.552) with red band was applied to generate phytomass and carbon distribution map of the study area.Banko Janakari, Vol. 24, No. 1, pp. 34-40


2021 ◽  
Vol 4 ◽  
Author(s):  
Christina Wiebe ◽  
Petra Nowak ◽  
Hendrik Schubert

Assessing the biodiversity of an ecosystem plays a major role in ecosystem management. However, proper determination on species-level is often tricky when morphological features are scarce and especially rare species require huge sampling efforts to be detected in the aquatic realm. As an alternative to conventional methods, environmental samples can be examined via the eDNA method, allowing for large-scale integration as well as taxa resolution independent from expression of morphological characters. However, to apply this technique genetic markers that are specific to a species or at least a genus are required. Such markers until now have been successfully developed only for a few well studied taxonomic groups like, e.g., fishes and amphibians, but are still missing for others, especially plants and algae (e.g. Bista et al. 2017). This project focusses on the development of species-specific markers for the macrophytic green algae Tolypella canadensis (Characeae, Charophyta), a rare alga preferring deep water and known so far mainly from remote places. Tolypella canadensis is a circumpolar species and prefers oligotrophic lakes, where it grows in depths up to 13 m (Langangen 2002; Romanov and Kopyrina 2016). In addition, proper determination of Tolypella-species is a field of a few specialists, further complicating monitoring or even detection of this rare species. The design of the species-specific primers was based on reference nucleotide sequences of the chloroplast genes rbcL, psbC and atpB and of the ribosomal internal transcribed spacer regions ITS1 and ITS2, obtained from GenBank (Perez et al. 2017). To determine the specificity of the newly designed primers, DNA isolates obtained from T. canadensis specimens collected from the Torneträsk (Sweden, 2018) and other charophyte species were prepared in different proportions. The sensitivity of the primers was experimentally assayed by using serial dilutions of T. canadensis DNA. Additionally, a mock test comprised of a sample with the DNA of several charophyte species was conducted and finally, the markers were tested on environmental samples from the Torneträsk. Tolypella canadensis-specific primers of the ITS2 region yielded positive PCR amplifications of one single band when T. canadensis was present in a sample. Cross-amplification was not found during the mock test; other charophyte species did not yield positive amplification. The eDNA samples from the Torneträsk validated the performance of the ITS2 marker. The T. canadensis-specific marker designed in this project was proven to be sensitive and accurate. It could be recommended as a useful tool to detect the presence of T. canadensis DNA, even at low concentration and in complex samples containing other charophyte species.


2020 ◽  
Vol 47 (4) ◽  
pp. 338
Author(s):  
Bracy W. Heinlein ◽  
Rachael E. Urbanek ◽  
Colleen Olfenbuttel ◽  
Casey G. Dukes

Abstract ContextCamera traps paired with baits and scented lures can be used to monitor mesocarnivore populations, but not all attractants are equally effective. Several studies have investigated the efficacy of different attractants on the success of luring mesocarnivores to camera traps; fewer studies have examined the effect of human scent at camera traps. AimsWe sought to determine the effects of human scent, four attractants and the interaction between attractants and human scent in luring mesocarnivores to camera traps. Methods We compared the success of synthetic fermented egg (SFE), fatty acid scent (FAS) tablets, castor oil, and sardines against a control of no attractant in luring mesocarnivores to camera traps. We deployed each attractant and the control with either no regard to masking human scent or attempting to restrict human scent for a total of 10 treatments, and replicated treatments eight to nine times in two different phases. We investigated whether: (1) any attractants increased the probability of capturing a mesocarnivore at a camera trap; (2) not masking human scent affected the probability of capturing a mesocarnivore at a camera trap; and (3) any attractants increased the probability of repeat detections at a given camera trap. We also analysed the behaviour (i.e. speed and distance to attractant) of each mesocarnivore in relation to the attractants. Key resultsSardines improved capture success compared with the control treatments, whereas SFE, castor oil, and FAS tablets had no effect when all mesocarnivores were included in the analyses. Masking human scent did not affect detection rates in the multispecies analyses. Individually, the detection of some species depended on the interactions between masking (or not masking) human scent and some attractants. ConclusionsSardines were the most effective as a broad-based attractant for mesocarnivores. Mesocarnivores approached traps baited with sardines at slower rates, which allows for a higher success of capturing an image of the animal. ImplicationsHuman scent may not need to be masked when deploying camera traps for multispecies mesocarnivore studies, but researchers should be aware that individual species respond differently to attractants and may have higher capture success with species-specific attractants.


2011 ◽  
Vol 92 (5) ◽  
pp. 1043-1055 ◽  
Author(s):  
Katerina Sevastou ◽  
Paulo Henrique Costa Corgosinho ◽  
Pedro Martínez Arbizu

A new species of the genus Dahmsopottekina is described from the Mediterranean Sea. Dahmsopottekina guilvardi sp. nov. was collected from abyssal habitats at a depth range of 2340–2850 m. Like its congeners, the new species has a vermiform habitus, a highly transformed P1 in both sexes and a plough-like rostrum in the female. Dahmsopottekina guilvardi sp. nov. can be distinguished from its congeneric species by the combination of a fused basis and endopodite in P1 of both sexes and the absence of an endopodite in P2–P4 of the female. Dahmsopottekina guilvardi sp. nov. is the second record of a harpacticoid species after its congener D. peruana in which the basis and endopodite of a leg other than the P5, namely the P1, are fused. Furthermore, the new species is the only one among Dahmsopottekina species with a 1-segmented P1 exopodite in the male. Similar to its congeners, D. guilvardi sp. nov. is strongly sexually dimorphic. This is evident through the morphology of most of the cephalic appendages and the reduction of P2–P6 in the female. The results of the present study support the observation that Dahmsopottekina species are sparsely distributed and highly endemic. Nevertheless, our results do not agree with the statement of considerably larger females as the length variability between females is greater than between the two sexes. Despite the morphological characters of the species commensurate with a burrowing mode of life, its presence in sediment traps suggests that D. guilvardi sp. nov. is an active ‘swimmer’.


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