Cyanidin 3-O-glucoside Chloride Attenuates Streptococcus suis-Induced Inflammation by Inhibiting MAPK and NF-κB Signaling Pathways in Murine Macrophage J774 Cells

2019 ◽  
Author(s):  
Xue Shen ◽  
Gen Li ◽  
Xuming Deng ◽  
Jianfeng Wang ◽  
Peng Zhang
Keyword(s):  
Signaling Pathways ◽  
Streptococcus Suis ◽  
Murine Macrophage ◽  
J774 Cells

Regulation of intracellular pH in J774 murine macrophage cells: H+ extrusion processes

1995 ◽  
Vol 268 (1) ◽  
pp. C210-C217 ◽  
Author(s):  
L. C. McKinney ◽  
A. Moran
Keyword(s):  
Intracellular Ph ◽  
Initial Rate ◽  
Adherent Cells ◽  
Murine Macrophage ◽  
Bafilomycin A1 ◽  
Macrophage Cell ◽  
Recovery From Acidification ◽  
J774 Cells ◽  
Acid Load ◽  
Rate Of Recovery

Mechanisms of intracellular pH (pHi) regulation were characterized in the murine macrophage cell line J774.1, using 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein to measure pHi. Under nominally HCO3(-)-free conditions, resting pHi of nonadherent J774.1 cells was 7.53 +/- 0.02 (n = 86), and of adherent cells was 7.59 +/- 0.02 (n = 97). In the presence of HCO3-/CO2, pHi values were reduced to 7.41 +/- 0.02 (n = 12) and 7.40 +/- 0.01 (n = 28), respectively. Amiloride, an inhibitor of Na+/H+ exchange, did not affect resting pHi. Inhibitors of a vacuolar type H(+)-ATPase [bafilomycin A1, N-ethylmaleimide (NEM), 7-chloro-4-nitrobenz-2-oxa-1,3-diazide (NBD), and p-chloromercuriphenylsulfonic acid (pCMBS)] reduced pHi by at least 0.2 pH units. Inhibitors of other classes of H(+)-ATPases (oligomycin, azide, vanadate, and ouabain) were without effect. Inhibition of H+ efflux, measured by the change in extracellular pH of a weakly buffered cell suspension, followed the same pharmacological profile, indicating that the reduction of pHi was due to inhibition of H+ extrusion. Mechanisms of recovery from an imposed intracellular acid load were also investigated. In NaCl-Hanks' solution, pHi recovered exponentially to normal within 2 min. The initial rate of recovery was inhibited > 90% by amiloride or by replacement of extracellular Na+ concentration by N-methyl-glucamine. Inhibitors of the vacuolar H(+)-ATPase also inhibited recovery. NEM and NBD nonspecifically inhibited all recovery. Bafilomycin A1 and pCMBS did not inhibit the initial amiloride-sensitive portion of recovery, but they did inhibit a late component of recovery when pHi was above 7.0. We conclude that the Na+/H+ exchanger is primarily responsible for recovery from an acid load but does not regulate resting pHi. Conversely, a vacuolar H(+)-ATPase regulates the resting pHi of J774 cells but contributes little to recovery from acidification.

Induction of NG-monomethyl-L-arginine uptake: a mechanism for differential inhibition of NO synthases?

1995 ◽  
Vol 269 (3) ◽  
pp. C750-C756 ◽  
Author(s):  
R. G. Bogle ◽  
R. J. MacAllister ◽  
G. S. Whitley ◽  
P. Vallance
Keyword(s):  
Culture Medium ◽  
Vascular Endothelial Cell ◽  
Cell Types ◽  
No Synthase ◽  
Vascular Endothelial ◽  
Murine Macrophage ◽  
Temperature Dependent ◽  
Arginine Uptake ◽  
J774 Cells ◽  
Fold Excess

The properties, selectivity, and regulation of NG-monomethyl-L-arginine (L-NMMA) uptake were examined in a human cultured vascular endothelial cell line SGHEC-7 and murine macrophage J774 cells. In both cell types the uptake of L-[14C]NMMA was time and temperature dependent. In endothelial cells L-[14C]NMMA uptake occurred via a single saturable carrier-mediated system with an apparent Kt of 77 +/- 2 microM. In murine macrophage cells a saturable component with an apparent Kt of 51 +/- 6 microM and a nonsaturable component of L-NMMA uptake were identified. In both cell types uptake of L-[14C]NMMA (10 microM) was significantly inhibited in the presence of 100-fold excess of L-NMMA, asymmetric NG,NG-dimethyl-L-arginine (ADMA), symmetric NG,NG-dimethyl-L-arginine (SDMA), L-canavanine, L-arginine, and to a lesser extent D-arginine. Uptake of L-[14C]NMMA was inhibited weakly (approximately 30%) by NG-nitro-L-arginine, NG-nitro-L-arginine methyl ester, aminoguanidine, and L-citrulline. Incubation of macrophage J774 cells with lipopolysaccharide (LPS; 1 or 10 micrograms/ml) resulted in the induction of nitric oxide (NO) synthase activity determined by the accumulation of nitrite in the culture medium. In these cells an enhanced uptake of L-NMMA uptake was observed which was prevented by pretreatment with cycloheximide (1 microM) but not dexamethasone (1 microM).(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Virulence properties and integron-associated antibiotic resistance of Klebsiella mobilis strains isolated from clinical specimens

2011 ◽  
Vol 60 (3) ◽  
pp. 281-288 ◽  
Author(s):  
Ryszard Koczura ◽  
Joanna Mokracka ◽  
Sylwia Krzymińska ◽  
Adam Kaznowski
Keyword(s):  
Antibiotic Resistance ◽  
Epithelial Cells ◽  
Cytotoxic Activity ◽  
Dna Fragmentation ◽  
Murine Macrophage ◽  
Gene Encoding ◽  
Clinical Specimens ◽  
Class 1 ◽  
J774 Cells ◽  
Virulence Properties

This study examined Klebsiella mobilis isolates cultured from clinical specimens for virulence-associated properties and antibiotic resistance. The strains produced a number of siderophores, including enterobactin, aerobactin and yersiniabactin. All isolates were able to adhere to and invade epithelial cells. They had cytotoxic activity, which caused destruction of human laryngeal epithelial HEp-2 cells and evoked lysis of murine macrophage J774 cells. Analyses of HEp-2 and J774 cellular morphology and DNA fragmentation in the cells showed features typical of cells undergoing apoptosis. Some K. mobilis strains harboured class 1 integrons carrying the aadA1 gene encoding an aminoglycoside adenyltransferase.

scholarly journals l-arginine transport is increased in macrophages generating nitric oxide

1992 ◽  
Vol 284 (1) ◽  
pp. 15-18 ◽  
Author(s):  
R G Bogle ◽  
A R Baydoun ◽  
J D Pearson ◽  
S Moncada ◽  
G E Mann
Keyword(s):  
Nitric Oxide ◽  
Time Dependent ◽  
Murine Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Arginine Transport ◽  
Nitrite Production ◽  
J774 Cells ◽  
Elevated Rate ◽  
Stimulation Of

Transport of L-arginine and nitrite production were examined in the murine macrophage cell line J774. Bacterial lipopolysaccharide (LPS) induced a dose- and time-dependent stimulation of nitrite production, which was further increased in the presence of interferon-gamma. Nitrite synthesis was absolutely dependent on extracellular L-arginine and inhibited in the presence of L-lysine or L-ornithine. In unactivated J774 cells L-arginine transport was saturable, with an apparent Km of 0.14 +/- 0.04 mM and Vmax. of 15 +/- 2 nmol/h per 10(6) cells. LPS (1 microgram/ml) induced a time-dependent stimulation of L-arginine transport, and after 24 h the Vmax. increased to 34 +/- 2 nmol/h per 10(6) cells. These findings indicate that activation of J774 cells with LPS produces an increase in both L-arginine transport and nitrite synthesis. The elevated rate of L-arginine transport in activated J774 cells may provide a mechanism for sustained substrate supply during enhanced utilization of L-arginine for the generation of NO.

scholarly journals Proteomics analysis of important molecules in serum from meningitic piglets caused by Streptococcus suis serotype 2

2020 ◽  
Vol 14 (05) ◽  
pp. 502-510
Author(s):  
Hexiang Jiang ◽  
Jianan Liu ◽  
Tong Wu ◽  
Mengmeng Liu ◽  
Qiang Sun ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Comparative Analysis ◽  
Signaling Pathways ◽  
Streptococcus Suis ◽  
Signal Pathways ◽  
Serum Samples ◽  
Serum Proteomics ◽  
C And N ◽  
Suis Serotype ◽  
Streptococcus Suis Serotype 2

Introduction: Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen that causes meningitis in China. This study’s aim was comparative analysis of serum proteomics from meningitis and non-meningitis piglets. Methodology: SS2 meningitis and non-meningitis piglet models were established. The serum samples were collected and analyzed by label-free LC-MS/MS proteomics technology. Differentially expressed proteins (DEPs) from serum were screened out by comparing the meningitis group and non-meningitis group to the healthy group (M/C; N/C), respectively. And then, globally and comparative analysis of DEPs in “M/C” and “N/C” in serum were performed using bioinformatics method. Finally, we comparatively analyzed the serum and cerebrospinal fluid proteomics in piglets that lived with meningitis. Results: We obtained 316 and 191 DEPs from “M/C” and “N/C” which classification visualizations were established. 157 DEPs were common in both groups and 159 DEPs were unique to the “M/C”. These DEPs and the signaling pathways which they participated in were visualized. Moreover, some DEPs which participated in multiple pathways were discovered and the interaction between 159 DEPs was also mapped. 39 common DEPs were also screened out in serum and cerebrospinal fluid during meningitis, and signaling pathways associated with these DEPs were further visualized. Conclusions: DEPs in “M/C” and “N/C” were comparatively analyzed and the similarities and differences of these DEPS which were involved in signal pathways were summarized. Moreover, several important molecules were screened out.

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