Enhanced Lipid Production of Chlorella sp. HS2 Using Serial Optimization and Heat Shock

There are numerous strains of Chlorella with a corresponding variety of metabolic pathways. A strain we previously isolated from wastewater in northern Sweden can grow heterotrophically as well as autotrophically in light and has higher lipid contents under heterotrophic growth conditions. The aims of the present study were to characterize metabolic changes associated with the higher lipid contents in order to enhance our understanding of lipid production in microalgae and potentially identify new compounds with utility in sustainable development. Inter alia, the amino acids glutamine and lysine were 7-fold more abundant under heterotrophic conditions, the key metabolic intermediate alpha-ketoglutarate was more abundant under heterotrophic conditions with glucose, and maltose was more abundant under heterotrophic conditions with glycerol than under autotrophic conditions. The metabolite 3-hydroxy-butyric acid, the direct precursor of the biodegradable plastic PHB (poly-3-hydroxy-butyric acid), was also more abundant under heterotrophic conditions. Our metabolomic analysis has provided new insights into the alga’s lipid production pathways and identified metabolites with potential use in sustainable development, such as the production of renewable, biodegradable plastics, cosmetics, and nutraceuticals, with reduced pollution and improvements in both ecological and human health.

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Cultivation of Chlorella sp. with livestock waste compost for lipid production

Bioresource Technology ◽

10.1016/j.biortech.2016.09.094 ◽

2017 ◽

Vol 223 ◽

pp. 296-300 ◽

Cited By ~ 31

Author(s):

L.-D. Zhu ◽

Z.-H. Li ◽

D.-B. Guo ◽

F. Huang ◽

Y. Nugroho ◽

...

Keyword(s):

Lipid Production ◽

Livestock Waste ◽

Chlorella Sp

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Microalgae Cultivation in Different pH, Temperature and Media for Lipid Production

International Journal of Life Sciences ◽

10.3126/ijls.v8i2.10227 ◽

2014 ◽

Vol 8 (2) ◽

pp. 13-17 ◽

Cited By ~ 1

Author(s):

Dakshayini Jayaramareddy ◽

Ravikumar Krishnappa ◽

Girisha Sirangala Thimmappa

Keyword(s):

Lipid Production ◽

Life Sciences ◽

Nutrient Supply ◽

Polar Lipids ◽

Microalgae Cultivation ◽

Microalgal Biomass ◽

Chlorella Sp ◽

Nonpolar Lipids

Lipids produced by microalgal biomass can be grouped into nonpolar lipids and polar lipids, which can be easily converted into biofuels. Microalgal samples were collected from three different ponds of Bangalore and cultured in the laboratory to find the effect of different pH, temperature and media on the production of biomass and lipids. Among these, pH-9, temperature -25°C and Beneck’s media was most suitable for production of biomass (35.80 g/L) and lipids from the isolated microalgae Chlorella sp. compare to Chladospora sp. (13.33 g/L). Chlorella sp. Showed 0.32 (OD) at pH-9, 0.43 (OD) at temperature-25°C and 2.94 (OD) in Beneck’s media. Our result revealed that nutrient supply along with measured variables affects the production of biomass and lipids in different microalgae.DOI: http://dx.doi.org/10.3126/ijls.v8i2.10227 International Journal of Life Sciences Vol.8(2): 2014; 13-17

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Statistical Approach of Nutrient Optimization for Microalgae Cultivation

E3S Web of Conferences ◽

10.1051/e3sconf/202014103009 ◽

2020 ◽

Vol 141 ◽

pp. 03009

Author(s):

Pichayatorn Bunkaew ◽

Sasithorn Kongruang

Keyword(s):

Light Intensity ◽

Cobalt Chloride ◽

Lipid Production ◽

Biodiesel Production ◽

Mixotrophic Cultivation ◽

Microalgae Cultivation ◽

Cultivation Time ◽

Chlorella Sp ◽

Chloride Hexahydrate ◽

Cobalt Chloride Hexahydrate

The Plackett-Burman Design (PBD) was applied to study fresh water microalgae cultivation using Chlorella sp. TISTR 8411 to select the influential nutrient factors for biomass and lipid production. The PBD for 13 trials from 11 nutrient factors with 3 levels was studied in the mixotrophic cultivation at 28 0C under 16:8 light and dark photoperiods over 7 days of cultivation time. Two influential factors were chosen as glucose and cobalt chloride hexahydrate to further design via Box-Behnken Design (BBD) in order to optimize the cultivation of this microalgae for biodiesel production. The 17 trials of 3 factors and 3 levels of BBD experimental design technique were applied with varying factors of glucose (20-40 g/L), cobalt chloride hexahydrate (0.01-0.04 mg/L) and light intensity (4,500-7,500 Lux) under 16:8 light and dark photoperiods over 7 days of cultivation time at 28 0C. Result showed that Chlorella sp. TISTR 8411 cultivation yield 0.52 g/L biomass and 0.31 g/L lipid production resulting in approximately 60% of lipid production when cultivated in 20.05 g/L glucose, 0.04 mg/L CoCl26H2O under light intensity of 4,614 Lux with the supplementation of 4.38 g/L NaHCO3 coupled with 1 g/L of both NaNO3 and KH2PO4. Under statically mixotrophic cultivation, result indicated that Chlorella sp. TISTR 8411 had potential to produce high lipid content for biodiesel application and biomass production for nutraceutical application. Further experiment with the longer cultivation period up to 2 weeks would implement not only for monitoring the growth kinetics but also evaluating the suitable type of fatty acid production.

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Bioremediation of Pyropia-processing wastewater coupled with lipid production using Chlorella sp.

Bioresource Technology ◽

10.1016/j.biortech.2020.124428 ◽

2021 ◽

Vol 321 ◽

pp. 124428

Author(s):

Shiyan Zheng ◽

Shanyi Chen ◽

Shangyun Zou ◽

Yiwen Yan ◽

Guang Gao ◽

...

Keyword(s):

Lipid Production ◽

Chlorella Sp

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Magnesium aminoclay enhances lipid production of mixotrophic Chlorella sp. KR-1 while reducing bacterial populations

Bioresource Technology ◽

10.1016/j.biortech.2016.08.034 ◽

2016 ◽

Vol 219 ◽

pp. 608-613 ◽

Cited By ~ 27

Author(s):

Bohwa Kim ◽

Ramasamy Praveenkumar ◽

Jiye Lee ◽

Bora Nam ◽

Dong-Myung Kim ◽

...

Keyword(s):

Lipid Production ◽

Bacterial Populations ◽

Chlorella Sp

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Characterization Chlorophytas microalgae with potential in the production of lipids for biofuels

CT&F - Ciencia Tecnología y Futuro ◽

10.29047/01225383.210 ◽

2012 ◽

Vol 5 (1) ◽

pp. 93-102 ◽

Cited By ~ 2

Author(s):

Denis-Lorena Jaimes-Duarte ◽

Wilder Soler-Mendoza ◽

Josman Velasco-Mendoza ◽

Yaneth Muñoz-Peñaloza ◽

Néstor-Andrés Urbina-Suárez

Keyword(s):

Biomass Production ◽

Lipid Content ◽

Lipid Production ◽

Biomass Concentration ◽

Production Yield ◽

Selective Media ◽

Media Control ◽

Chlorella Sp ◽

Nutritional Conditions ◽

Potential Applications

This work is part of a megaproject that seeks to isolate microalgae of the Chlorophyta division native to Norte de Santander and identify their potential applications such as lipid production to be used as biofuel. Here we present the isolation of 11 microalgae strains from the Chlorophyta division found in two different wastewater environments. The collected strains were cultivated in selective media and purified through serial dilutions, depletion culture, and application of penicillin and gentamicin. Biomass production was evaluated and two strains were selected: CHL1 (Chlorella sp.) and DES1 (Desmodesmus sp.). The strains were cultivated on wastewater and PCG media (control), and their biomass concentration and lipid content were measured. Both strains reached similar biomass concentrations compared to their respective controls (CHL1 PCG 1.5 mg/L ± 0.035 mg/L, CHL1 AR 1.68 mg/L ± 0.036, DES1 PCG 1.66 mg/L ± 0.007, DES1 AR 2 mg/L ± 0.03) and their lipid content was slightly higher compared to their controls. The results show that the isolated and evaluated strains may have potential to be lipid producers, since their environmental and nutritional conditions have not been modified yet and adaptation may improve the production yield of lipids.

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Synthesis of neutral lipids in chlorella sp. under different light and carbonate conditions

CT&F - Ciencia Tecnología y Futuro ◽

10.29047/01225383.228 ◽

2011 ◽

Vol 4 (4) ◽

pp. 47-57 ◽

Cited By ~ 15

Author(s):

Jazmín-Vanessa Pérez-Pazos ◽

Pablo Fernández-Izquierdo

Keyword(s):

Neutral Lipids ◽

Lipid Production ◽

Red Light ◽

Lipid Synthesis ◽

Basal Medium ◽

Chlorella Sp ◽

High Lipid ◽

The Right ◽

Two Stages ◽

Physical And Chemical

Lipids are biomolecules of great scientific and biotechnological interest due to their extensive applications. Microalgae are potential biological systems used in the synthesis of lipids, particularly Chlorella sp., which is characterized by its high lipid content and for having the right profile for the obtainment of biofuel. Lipid production in microalgae is influenced by several physical and chemical factors. Any modification thereof can cause a stress response represented by changes in synthesized lipid composition, varying from one species to another. This paper evaluates the effect of different light wavelengths, photoperiods and calcium carbonate (CaCO3) supply in lipid synthesis in Chlorella sp. In order to do so, the microalgae was grown in Bold's Basal Medium (BBM) at 20ºC with constant aeration and subject to low blue (470 nm) and red (700 nm) light wavelengths, 0,5 g.L-1 and 1,5 g.L-1 concentrations of CaCO3 and 6-hour light, 18-hour darkness (6:18) and 18-hour light, 6-hour darkness (18:6) photoperiods. The results indicate a higher growth rate for microalgae under red light, 0,5 g.L-1 of CaCO3 and a photoperiod of 6:18. On the other hand, lipid production is higher under blue light, 1,5 g.L-1 of CaCO3 and an18:6 photoperiod. Analysis by gas chromatography indicate that the fatty acids in the samples are oleic, linoleic and palmitoleic, which are of recognized importance in the biodiesel industry. This suggests that neutral lipid synthesis can be optimized in two stages: first, by promoting growth and subsequently, by inducing lipid production.

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