Advanced Approaches to Diagnose and Treat the Chronic Autoimmune Disorders

2016 ◽  
pp. 70-98
Author(s):  
Tomasz Sołtysiński
Keyword(s):  
Immune System ◽  
Imaging Spectroscopy ◽  
Ultrasonic Waves ◽  
Optical Methods ◽  
Label Free ◽  
Experimental Systems ◽  
Cell Measurement ◽  
Living Organisms ◽  
On Chip ◽  
Molecular Components

Pathology and dynamics of particular cells and the molecular components of immune system is still challenging to be traced within living organisms. The techniques of molecular imaging (MI) are promising tools to monitor the immune system at work, to improve or allow personalized diagnostics and treatment, especially of the autoimmune diseases. In this study some possible targets for MI and biosensing are discussed. The personalized medicine, in addition to bioinformatics-based systemic approach, requires extensive research and novel high-throughput technologies like next generation of imaging, biosensing experimental systems based on microfluidics, nanotechnology, femtochemistry, superresolution (STED, STORM, PALM, SOFI, etc.), label-free imaging, spectroscopy (including TCSPC), MRI, multimodal optical methods, accoustic imaging through ultrasonic waves, nuclear medicine methods like SPECT and PET. Moreover, dedicated designs of modular Lab-on-Chip solutions are of high demand to perform multipurpose cell measurement and give a possibility to flexibly interact with sensed objects.

Advanced Approaches to Diagnose and Treat the Chronic Autoimmune Disorders

2017 ◽  
pp. 1741-1770
Author(s):  
Tomasz Sołtysiński
Keyword(s):  
Immune System ◽  
Imaging Spectroscopy ◽  
Ultrasonic Waves ◽  
Optical Methods ◽  
Label Free ◽  
Experimental Systems ◽  
Cell Measurement ◽  
Living Organisms ◽  
On Chip ◽  
Molecular Components

Pathology and dynamics of particular cells and the molecular components of immune system is still challenging to be traced within living organisms. The techniques of molecular imaging (MI) are promising tools to monitor the immune system at work, to improve or allow personalized diagnostics and treatment, especially of the autoimmune diseases. In this study some possible targets for MI and biosensing are discussed. The personalized medicine, in addition to bioinformatics-based systemic approach, requires extensive research and novel high-throughput technologies like next generation of imaging, biosensing experimental systems based on microfluidics, nanotechnology, femtochemistry, superresolution (STED, STORM, PALM, SOFI, etc.), label-free imaging, spectroscopy (including TCSPC), MRI, multimodal optical methods, accoustic imaging through ultrasonic waves, nuclear medicine methods like SPECT and PET. Moreover, dedicated designs of modular Lab-on-Chip solutions are of high demand to perform multipurpose cell measurement and give a possibility to flexibly interact with sensed objects.

scholarly journals Pivoting the Plant Immune System from Dissection to Deployment

Science ◽  
2013 ◽  
Vol 341 (6147) ◽  
pp. 746-751 ◽  
Author(s):  
Jeffery L. Dangl ◽  
Diana M. Horvath ◽  
Brian J. Staskawicz
Keyword(s):  
Immune System ◽  
Conceptual Understanding ◽  
Germ Plasm ◽  
Plant Diseases ◽  
Sequencing Technology ◽  
Plant Immune System ◽  
Rich Diversity ◽  
The World ◽  
The Rich ◽  
Molecular Components

Diverse and rapidly evolving pathogens cause plant diseases and epidemics that threaten crop yield and food security around the world. Research over the last 25 years has led to an increasingly clear conceptual understanding of the molecular components of the plant immune system. Combined with ever-cheaper DNA-sequencing technology and the rich diversity of germ plasm manipulated for over a century by plant breeders, we now have the means to begin development of durable (long-lasting) disease resistance beyond the limits imposed by conventional breeding and in a manner that will replace costly and unsustainable chemical controls.

ИЗМЕНЕНИЯ ИММУНОЛОГИЧЕСКОГО ОТВЕТА В РЕАКЦИИ НСТ С ПЕРИТОНЕАЛЬНЫМИ МАКРОФАГАМИ ПОД ДЕЙСТВИЕМ ШИРОКОПОЛОСНОГО ЭЛЕКТРОМАГНИТНОГО ИЗЛУЧЕНИЯ В ЭКСПЕРИМЕНТЕ

Innova ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 55-57
Author(s):  
Андрей Сергеевич Руцкой ◽  
◽  
Вячеслав Александрович Липатов ◽  
Александр Анатольевич Панов ◽  
Илья Игоревич Шляпцев ◽  
...  
Keyword(s):  
Immune System ◽  
Enzymatic Activity ◽  
Electromagnetic Fields ◽  
Peritoneal Macrophages ◽  
Nitro Blue Tetrazolium ◽  
Blue Tetrazolium ◽  
Living Organisms ◽  
Different Characteristics

The article presents the results of research on the study of the effect of broadband electromagnetic fields on a macroorganism in an experiment. Electromagnetic fields with different characteristics can affect the systems of living organisms in different ways, including the immune system, which is sensitive to electromagnetic influences. Broadband electromagnetic fields have an immunostimulating effect, as evidenced by the increase in the enzymatic activity of peritoneal macrophages in reaction with nitro blue tetrazolium.

scholarly journals Volumetric registration of magnetic nanoparticles for optimization of quantitative immunochromatographic assays for detection of small molecules

2018 ◽  
Vol 185 ◽  
pp. 10006 ◽  
Author(s):  
Natalia V. Guteneva ◽  
Sergey L. Znoyko ◽  
Alexey V. Orlov ◽  
Maxim P. Nikitin ◽  
Petr I. Nikitin
Keyword(s):  
Magnetic Nanoparticles ◽  
Small Molecules ◽  
Optical Methods ◽  
Magnetic Method ◽  
Label Free ◽  
Spectral Correlation ◽  
Entire Volume ◽  
Specificity And Sensitivity ◽  
Particle Quantification

Precise quantitative and highly sensitive detection of small molecules (haptens) is highly demanded in medicine, food quality control, in vitro diagnostics, criminalistics, environmental monitoring, etc. In the present work, the magnetic method of particle quantification and the optical methods of spectral correlation and spectral phase interferometry complement each other for optimization of a quantitative assay for measuring concentrations of small molecules. The assay employs magnetic nanoparticles as labels in rapid immunochromatographic format. The approach was demonstrated with fluorescein as a model molecule. The interferometric label-free biosensors were employed for selection of optimal reagents that produced high specificity and sensitivity. The method of magnetic particle quantification counted the magnetic labels over the entire volume of the immunochromatographic membrane to provide their distribution along the test strip. Such distribution was used for optimization of such parameters as concentrations of the used reagents and of antibody immobilized on the labels, amount of the labels and conjugates of haptens with protein carriers to realize the advanced quantitative immunochromatographic assay.

scholarly journals Acute On-Chip HIV Detection Through Label-Free Electrical Sensing of Viral Nano-Lysate

Small ◽  
2013 ◽  
Vol 9 (15) ◽  
pp. 2553-2563 ◽  
Author(s):  
Hadi Shafiee ◽  
Muntasir Jahangir ◽  
Fatih Inci ◽  
ShuQi Wang ◽  
Remington B. M. Willenbrecht ◽  
...  
Keyword(s):  
Label Free ◽  
Electrical Sensing ◽  
On Chip

Establishment of screening system toward discovery of kinase inhibitors using label-free on-chip phosphorylation assays

Biosystems ◽  
2009 ◽  
Vol 97 (3) ◽  
pp. 179-185 ◽  
Author(s):  
Kazuki Inamori ◽  
Motoki Kyo ◽  
Kazuki Matsukawa ◽  
Yusuke Inoue ◽  
Tatsuhiko Sonoda ◽  
...  
Keyword(s):  
Kinase Inhibitors ◽  
Screening System ◽  
Label Free ◽  
On Chip

scholarly journals Plasmonic nanoparticles-decorated diatomite biosilica: extending the horizon of on-chip chromatography and label-free biosensing

2017 ◽  
Vol 10 (11) ◽  
pp. 1473-1484 ◽  
Author(s):  
Xianming Kong ◽  
Erwen Li ◽  
Kenny Squire ◽  
Ye Liu ◽  
Bo Wu ◽  
...  
Keyword(s):  
Plasmonic Nanoparticles ◽  
Label Free ◽  
On Chip

scholarly journals Dynamic Colonization of Microbes and Their Functions after Fecal Microbiota Transplantation for Inflammatory Bowel Disease

mBio ◽  
2021 ◽  
Author(s):  
Nathaniel D. Chu ◽  
Jessica W. Crothers ◽  
Le T. T. Nguyen ◽  
Sean M. Kearney ◽  
Mark B. Smith ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Immune System ◽  
Bowel Disease ◽  
Healthy Donor ◽  
Fecal Microbiota Transplantation ◽  
Fecal Microbiota ◽  
Complex Mixtures ◽  
Sick Patient ◽  
Inflammatory Bowel ◽  
Living Organisms

Fecal microbiota transplantation (FMT)—transferring fecal microbes from a healthy donor to a sick patient—has shown promise for gut diseases such as inflammatory bowel disease. However, unlike pharmaceuticals, fecal transplants are complex mixtures of living organisms, which must then interact with the microbes and immune system of the recipient.

scholarly journals Methods and applications of label-free cell-based systems

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Joachim Wiest
Keyword(s):  
Environmental Monitoring ◽  
Morphological Changes ◽  
Free Cell ◽  
Living Cells ◽  
Label Free ◽  
Controlled System ◽  
Chip Technology ◽  
Cell Monitoring ◽  
A Cell ◽  
On Chip

Label-free monitoring of living cells is used in various applications such as drug development, toxicology, regenerative medicine or environmental monitoring. The most prominent methods for monitoring the extracellular acidification, oxygen consumption, electrophysiological activity and morphological changes of living cells are described. Furthermore, the intelligent mobile lab (IMOLA) – a computer controlled system integrating cell monitoring and automated cell cultivation – is described as an example of a cell-based system for microphysiometry. Results from experiments in the field of environmental monitoring using algae are presented. An outlook toward the development of an organ-on-chip technology is given.

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