Interference Microscopy for Cellular Studies

Author(s):  
Alexey R. Brazhe ◽  
Nadezda A. Brazhe ◽  
Alexey N. Pavlov ◽  
Georgy V. Maksimov

This chapter describes the application of interference microscopy and double-wavelet analysis to noninvasive study of cell structure and function. We present different techniques of phase and interference microscopy and discuss how variations in the intrinsic optical properties of a cell can be related to the intracellular processes. Particular emphasis is given to the newly developed phase modulation laser interference microscope. We show how this setup, combined with wavelet analysis of the obtained data series, can be applied to live cell imaging to investigate the rhythmic intracellular processes and their mutual interactions. We hope that the discussion will contribute to the understanding and learning of new methods for non-invasive investigation of intracellular processes.

Author(s):  
Renato Paro ◽  
Ueli Grossniklaus ◽  
Raffaella Santoro ◽  
Anton Wutz

AbstractThe identity of cells in an organism is largely defined by their specific transcriptional profile. During cell division, these profiles need to be faithfully inherited to the daughter cells to ensure the maintenance of cell structure and function in a cell lineage. Here, you will learn how two groups of chromatin regulators, the Polycomb group (PcG) and the Trithorax group (TrxG), act in an antagonistic manner to maintain differential gene expression states. Members of the PcG cooperate in large multiprotein complexes to modify histones with repressive marks, resulting in condensed chromatin domains. Conversely, the TrxG proteins counteract the repressed domains by opening nucleosomal structures and establishing activating histone modifications. PcG and TrxG proteins are evolutionary highly conserved and control diverse processes, such as the identity of stem cells in mammalian development to the process of vernalization in plants.


Pancreas ◽  
2004 ◽  
Vol 29 (3) ◽  
pp. e77-e83 ◽  
Author(s):  
Sigrid A. Rajasekaran ◽  
Jegan Gopal ◽  
Cromwell Espineda ◽  
Sergey Ryazantsev ◽  
Eveline E. Schneeberger ◽  
...  

Author(s):  
Nugroho Budhiwaluyo ◽  
Rayandra Asyhar ◽  
Bambang Hariyadi

  This research aims to produce a final product in the form of a performance-assessment instrument on Cell Structure and Function experiment. The development model is ADDIE. Based on expert's judgment, the instrument was valid and can be tested in the field. Field-test results shown that the product performs high validity and reliability value on measuring student performance on Cell Structure and Function experiment. Therefore, it is concluded that this performance-assessment instrument theoretically and practically has a good quality for measuring student performance in both process and product performance on Cell Structure and Function experiment. Keywords: Development, Performance-Assessment Instrument, Cell Structure and Function Experiment 


Gene Therapy ◽  
2021 ◽  
Author(s):  
Shagana Visuvanathan ◽  
Adam N. Baker ◽  
Pamela S. Lagali ◽  
Stuart G. Coupland ◽  
Garfield Miller ◽  
...  

Author(s):  
Supriya Mahajan ◽  
Alexander Jacob ◽  
Anju Kelkar ◽  
Anthony Chang ◽  
Daniel Mcskimming ◽  
...  

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