scholarly journals Screening common bean germplasm for resistance to genetically diverse Sclerotinia sclerotiorum isolates from Argentina

2020 ◽  
Vol 42 ◽  
pp. e42786
Author(s):  
Carla Luciana Abán ◽  
Gisel Maria Taboada ◽  
Norma Beatriz Casalderrey ◽  
Maria Elisa Maggio ◽  
Mario Osvaldo Chocobar ◽  
...  

White mold caused by Sclerotinia sclerotiorum (Lib.) de Bary is a devastating disease that affects the common bean (Phaseolus vulgaris. L) crop worldwide. In Argentina, white mold has been detected in all bean production areas, reaching seed yield and quality losses up to 100% on susceptible common bean cultivars under favorable weather conditions. The aim of this study was to screen the physiological resistance of 20 common bean accessions to five genetically distinct isolates of S. sclerotiorum collected from the main common bean growing area of Argentina, using the greenhouse straw test. The white mold reaction was scored at 7, 14, and 21 days post-inoculation using a 1 (no disease symptoms) to 9 (severely diseased or dead plants) scale and the area under the disease progress curve (AUDPC) was determined. Highly significant differences (p < 0.001) were observed between isolates, accessions and genotype x isolate interaction at the three evaluations dates. All cultivars and lines were susceptible at the end of the assessment, except line A 195 which was resistant to white mold against the five isolates tested and was significantly different from all accessions. This work represents a valuable contribution to regional breeding programmes aimed to obtain cultivars with durable resistance.

2009 ◽  
Vol 89 (4) ◽  
pp. 755-762 ◽  
Author(s):  
H Terán ◽  
S P Singh

White mold (WM) caused by Sclerotinia sclerotiorum (Lib.) de Bary is the most devastating disease of common bean (dry and snap or garden bean) (Phaseolus vulgaris L.) in North America. The use of a reliable screening method (SM) in common bean is crucial to improve physiological resistance to WM. The objective of this study was to compare the efficacy of three SM to identify physiological resistance in dry bean genotypes with different evolutionary origins and levels of resistance. Screening methods tested were: (i) the modified straw test or cut–stem (CSM); (ii) infected bean flower (IFL); and (iii) infected oat seed (IOS). A 195, ICA Bunsi, Othello, and VCW 54 dry bean were tested with the three SM. The experimental design was a split plot in randomized complete blocks with three replications in 2007 and 2008. Two independent inoculations 1 wk apart for each SM were made. The WM reaction was scored at 16, 23, and 33 d post-inoculation (DPI) using a 1 to 9 scale. There were highly significant differences between SM and its interaction with years. The CSM and IFL were the most consistent and highly correlated (r > 0.70, P < 0.01). Interspecific breeding line VCW 54 consistently had the highest WM resistance across years, SM, and evaluation dates, followed by A 195. White mold scores increased with delayed evaluations. Thus, CSM or IFL with disease assessed 33 DPI should be used for identifying common bean genotypes with high levels of physiological resistance to WM.Key words: Common bean, growth habit, race Mesoamerica, race Nueva Granada, Phaseolus vulgaris, Sclerotinia sclerotiorum


2019 ◽  
Vol 43 ◽  
Author(s):  
Rafael Novais de Miranda ◽  
Caroline Marcela da Silva ◽  
Antonio Carlos da Mota Porto ◽  
Welison Andrade Pereira

ABSTRACT The Straw Test is an assay developed to evaluate the resistance of common bean to white mold, in which the plant stems are inoculated and the symptoms of the disease are monitored. It is plausible to admit that investigating gene expression in pathogen-infected tissues may be strategically interesting. However, obtaining a quality RNA is a basic requirement for this purpose. Therefore, the objective of this study was to evaluate adjustments in protocols of commercial kits in the expectation of improving the quality of RNA obtained from bean stems. For this, plants of two lines were inoculated and the stems pathogen-infected were collected 72 hours after. For RNA extraction, two commercial reagents were used following the manufacturer’s recommendations and then following adaptations in these protocols. In particular, the proposed modifications relate to volumes of supernatant recovered in purification steps, additional step of chloroform purification and extended time for nucleic acids precipitation. The obtained RNA was analyzed by spectrophotometer, electrophoresis and bioanalyzer, then converted into cDNA and subsequently submitted to PCR. From the obtained data, it was observed that the adaptations made in the protocols contributed to better results and that, when the indicative values of RNA quality are guaranteed, the subsequent reactions are more pure, precise and representative.


2021 ◽  
Vol 48 (6) ◽  
pp. 729-739
Author(s):  
María Fernanda Villarreal-Delgado ◽  
Fannie Isela Parra-Cota ◽  
Luis Alberto Cira-Chávez ◽  
María Isabel Estrada-Alvarado ◽  
Sergio de los Santos-Villalobos

2013 ◽  
Vol 35 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Luana da Silva Botelho ◽  
Willian Luis Antonio Zancan ◽  
José da Cruz Machado ◽  
Ellen Noly Barrocas

The fungus Sclerotinia sclerotiorum, causal agent of white mold disease on several economically important crops, such as bean, soybean, and cotton, is commonly disseminated through seeds and can cause high losses on their quality and in productivity of these species. The aim of this study was assessing the effects of different initial inoculums potentials of this fungus on common bean seeds using two different strains of the fungus and two genotypes of common beans (Pérola and Ouro Negro) artificially inoculated. Seeds were sown on soil and the cultivation was performed under controlled environmental conditions favorable to development of the white mold disease. Variables assessed were: germination; seed health; emergence speed index; initial and final seedling number; and dry weight of aerial parts and roots. It was found that with the gradual increase in inoculum potential in the seeds also occurred gradual reduction in the values of: germination; emergence speed index; seedling stand; and length and dry mass of aerial parts and roots. These results show the importance of initial inoculum potential of S. sclerotiorum present in common bean seeds, as much in disseminating the pathogen as on direct damages caused in field by reducing productive potential of the emerged plants.


Irriga ◽  
2010 ◽  
Vol 15 (2) ◽  
pp. 159-172 ◽  
Author(s):  
Fábio Venegas ◽  
João Carlos Cury Saad

 O presente estudo objetivou determinar a severidade da doença mofo branco (Sclerotinia sclerotiorum (Lib.) de Bary) e os componentes de produção do feijoeiro (Phaseolus vulgaris L.), var. Pérola, submetido a aplicação do fungicida procimidone (Sialex 500), via fungigação (pivô central) e pulverizador automotriz (Uniport). O estudo foi realizado sob condições de produção comercial em campo, no município de Primavera do Leste - MT.  O experimento constou de 5 tratamentos (com 4 repetições de 4 ha cada), sendo 4 deles envolvendo duas aplicações de procimidone (1,2 kg ha -1 cada aplicação), aos 42 dias e aos 52 dias após o plantio (DAP), e um deles sem aplicação deste produto, com delineamento experimental de blocos casualizados.  Foram testadas as lâminas de água de 5,5 e 11,0  mm na aplicação via pivô central, proporcionando assim volume de calda de 55.000 e 110.000 L ha -1, respectivamente, e os volumes de 120 e 200 L ha -1 de calda no pulverizador automotriz. O critério adotado em todas as avaliações da severidade da doença foi o da porcentagem da área foliar lesionada utilizando escala diagramática de severidade da doença mofo branco. Os  valores foram usados para calcular a área abaixo da curva de progresso de doença (AACPD). Foram analisados também, o número dos apotécios do fungo durante o ciclo da cultura e o peso dos escleródios residuais do patógeno na colheita. Nesta ocasião foram avaliados os seguintes componentes da produção da cultura: número de plantas por parcela (stand final), número de vagens por planta, número de grãos por vagem, peso médio de 200 grãos e produtividade de grãos. Os valores da AACPD, apotécios aos 42, 49 e 56 DAP, escleródios em 2 kg de solo e  os parâmetros de produtividade da cultura foram submetidos à análise de variância e ao teste de comparação de médias de Tukey no nível de 5 % de significância, utilizando o programa computacional STAT. Nas condições experimentais em que se desenvolveu o trabalho, não foram constatadas diferenças significativas entre as diferentes técnicas de aplicação estudadas em relação ao parâmetros de produtividade, porém, melhores resultados de controle da doença mofo branco, menor número de apotécios por m2 aos 49 e 56 DAP e menor peso dos escleródios residuais na colheita foram obtidos com a aplicação do fungicida via pivô central, independentemente do volume de calda utilizado.   UNITERMOS: Phaseolus vulgaris L., Sclerotinia sclerotiorum (Lib.) de Bary, fungigação.             VENEGAS, F; SAAD, J. C. C.  WHITE MOLD CONTROL USING FUNGIGATION AND YIELD BEAN IN BRAZILIAN SAVANNAH CONDITIONS.     2 ABSTRACT              The aim of this research was to evaluate the white mold severity (Sclerotinia sclerotiorum (Lib.) of Bary), bean production components and yield (Phaseolus vulgaris L.), variety Perola, according to the application of procimidone fungicide (Sialex 500), through fungigation (center pivot) and automotive sprayer (Uniport). The study was carried under field production commercial conditions, in Primavera do Leste - MT - Brazil. The experiment consisted of 5 treatments (with 4 repetitions of 4 ha each), all with two procimidone applications (1.2 kg ha-1 each application, same as, 0.6 kg a.i. per hectare) to the 42 and 52 days after seeding. The water depths of 5.5 and 11.0 mm were tested in the application through central pivot (this had your checked uniformity), providing volumes of 55.000 and 110.000 L ha-1, respectively,  and the volumes of 120 and 200 L ha-1 in the automotive sprayer. The severity of disease was evaluated by the percentage of the area affected by plant damage using diagramatic grade scale of white mold severity, as described by Azevedo (1998). The values were used to calculate the area under the disease progress curve (AUDPC). They were also analyzed, the number of the fungus apothecia during the crop cycle and the residual sclerotias weight in harvest. On this occasion, it was also evaluated the crop yield parameters: number of plants per plot (final stand), pods per plant, grains per pod, medium weight of 200 grains and productivity of grains. The AUDPC values, apothecia to 42, 49 and 56 days after seeding, sclerotias in 2 soil kg and the crop productivity parameters were submitted to the variance analysis and Tukey Test at 0.05 of probability. This test was also applied in the comparison among the different fungicide application methods, independent of spray volumes in each one. The statistical processing was accomplished by STAT program. The results showed that weren't differences among application techniques studied in relation to productivity parameters, however,  best white mold control, smaller apothecia number to 49 and 56 days after seeding and smaller weight of residual sclerotias in the harvest were obtained with the fungigation, independently of the spray volume used.   KEYWORDS: Phaseolus vulgaris L., Sclerotinia sclerotiorum (Lib.) de Bary, fungigation. 


2007 ◽  
Vol 8 ◽  
pp. 20-29 ◽  
Author(s):  
Hem R. Regmi

For the third consecutive year, drought conditions have been severely affecting agricultural production in Nepal and in some cases turning traditionally surplus production areas into deficit ones. In addition to the heavily drought affected Mid- and Far-Western Regions, flood exacerbated by hailstones and crop diseases in various VDCs caused extensive crop loss. Drought particularly affected the traditionally food surplus areas of the Eastern Terai, particularly Siraha, Saptari and Dhanusha which reported a decline in paddy production of about 30%. A total of 109,855 hectares of paddy land (7.09%) remained fallow in 2006/07 as farmers were unable to plant due to drought. In addition, yields were lower by almost 6%. Paddy crop production consequently declined by 12.5 % compared to last year. The total food grain shortfall for the year 2006/07 has been calculated at 187,748 Mt assuming favorable weather conditions for the winter crop wheat and barley. The Journal of AGRICLUTURE AND ENVIRONMENT Vol. 8, 2007, pp. 20-29


Plant Disease ◽  
2011 ◽  
Vol 95 (11) ◽  
pp. 1370-1377 ◽  
Author(s):  
Lindsey Otto-Hanson ◽  
James R. Steadman ◽  
Rebecca Higgins ◽  
Kent M. Eskridge

There is no complete resistance to Sclerotinia sclerotiorum, cause of white mold in dry bean (Phaseolus vulgaris). Variable resistance expression is one problem in screening for improved white mold resistance. With no previous information in the literature, pathogen variation in multisite screening nurseries was evaluated as one cause of diverse resistance expression. In all, 10 isolates of S. sclerotiorum used in greenhouse screening and 146 isolates collected from nine white mold field screening nurseries in major bean production areas in the United States were compared using mycelial compatibility groupings (MCGs) and an aggressiveness test. These 10 greenhouse screening isolates formed six MCGs. Among 156 field and greenhouse isolates, 64 MCGs were identified and 36 of those were each composed of a single unique isolate. Significant differences in isolate aggressiveness were found between some isolates in different MCGs but the isolates within an MCG did not differ in aggressiveness. High isolate variation found within and between field locations could influence the disease phenotype of putative white mold resistant germplasm. We next compared genotype and phenotype of isolates from screening nurseries and those from producer fields. Variability found in and among screening locations did reflect variability found in the four producer fields sampled. White mold resistance screening can be improved by knowledge of isolate genotypic and phenotypic characteristics.


Crop Science ◽  
2020 ◽  
Vol 60 (4) ◽  
pp. 1986-1996
Author(s):  
Sonali Chauhan ◽  
Shabnam Katoch ◽  
S.K. Sharma ◽  
P.N. Sharma ◽  
J.C. Rana ◽  
...  

2020 ◽  
Vol 41 (6) ◽  
pp. 2597-2612
Author(s):  
Douglas Casaroto Peitl ◽  
◽  
Ciro Hideki Sumida ◽  
Ricardo Marcelo Gonçalves ◽  
Sérgio Florentino Pascholati ◽  
...  

The antagonistic activity of 25 saprobe fungi from semiarid areas of Northeast Brazil was evaluated against Sclerotinia sclerotiorum (Lib.) de Bary (Helotiales: Sclerotiniaceae). Four fungi [Myrothecium sp. Tode (Hypocreales: Stachybotryaceae) isolate 2, Volutella minima Höhn. (Hypocreales: Nectriaceae), Phialomyces macrosporus P.C. Misra & P.H.B. Talbot (Pezizomycotina) and Dictyosporium tetraseriale Goh, Yanna & K.D. Hyde (Pleosporales: Dictyosporiaceae)] were selected and further tested their ability to inhibit mycelial growth, sclerotia formation and ascospore germination of S. sclerotiorum and to control white mold on soybean plants. V. minima and P. macrosporus filtrates at 50% effectively suppressed mycelial growth and Myrothecium sp. isolate 2 completely suppressed sclerotia formation and inhibited ascospore germination by over 95%, the same result as commercial fungicide fluazinam. Soybean plants pre-treated with Myrothecium sp. isolate 2, P. macrosporus, and V. minima and inoculated with S. sclerotiorum showed a reduction of 55.8%, 79.7%, and 83.2% of area under disease progress curve (AUDPC) of white mold, respectively, in relation to water. Collectively, these results underline the antagonistic activity of V. minima, P. macrosporus, and Myrothecium sp. isolate 2 against S. sclerotiorum and their potential as biocontrol agents of soybean white mold.


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