scholarly journals Influence of culture medium pH on the production of CGTase by Bacillus firmus Strain No. 37 - doi: 10.4025/actascitechnol.v35i3.15882

2013 ◽  
Vol 35 (3) ◽  
Author(s):  
Jéssica Bravin Carmello ◽  
Gisella Maria Zanin ◽  
José Eduardo Olivo
Keyword(s):  
Culture Medium ◽  
Bacillus Firmus ◽  
Medium Ph

scholarly journals Glycoprotein metabolism in normal and β-mannosidase-deficient cultured goat skin fibroblasts

1986 ◽  
Vol 234 (1) ◽  
pp. 175-183 ◽  
Author(s):  
L W Hancock ◽  
M Z Jones ◽  
G Dawson
Keyword(s):  
Culture Medium ◽  
Human Fibroblasts ◽  
Skin Fibroblasts ◽  
Medium Ph ◽  
Cytosolic Ph ◽  
Catabolic Pathways ◽  
No Conversion ◽  
Lysosomal Ph ◽  
Cultured Skin ◽  
Oligosaccharide Structures

Cultured skin fibroblasts established from goats affected with beta-mannosidosis, an inherited neurovisceral storage disorder, showed an absence of lysosomal beta-mannosidase activity and the corresponding accumulation of a trisaccharide (TS) with the structure Man beta (1→4)GlcNAc beta (1→4)GlcNAc (0.4 mumol/g) and lesser amounts (0.15 mumol/g) of a Man beta (1→4)GlcNAc disaccharide (DS). By using purified storage TS isolated from fibroblasts metabolically labelled with [3H]GlcN, no conversion of TS into DS could be demonstrated in homogenates of affected cells at either lysosomal pH (4.4) or cytosolic pH (6.1), or in the culture medium (pH 7.0) of affected cells. Both TS and DS were secreted into the culture medium by affected fibroblasts. When affected fibroblasts were treated with tunicamycin before labelling with [3H]GlcN, the accumulation of both labelled TS and DS was completely inhibited. Treatment of both affected and normal goat fibroblasts with swainsonine resulted in the inhibition of lysosomal alpha-mannosidase activity and in the accumulation of the same labelled oligosaccharides in both. The major storage pentasaccharide from both normal and affected swainsonine-treated fibroblasts was sensitive to digestion with alpha-mannosidase and endo-beta-N-acetylhexosaminidase D, suggesting a branched mannose structure and a chitobiose core. In the absence of evidence for the existence of unusual N-linked glycoprotein-associated chitotriose oligosaccharide structures in affected goat fibroblasts, it must be concluded that degradative pathways for N-linked oligosaccharides are similar in both normal and affected goat fibroblasts, and that these pathways differ from catabolic pathways in human fibroblasts.

Regeneration des Cortex bei Stentor coeruleus / Regeneration of the Cortex in Stentor coeruleus

1989 ◽  
Vol 44 (9-10) ◽  
pp. 876-882
Author(s):  
Viktor Schwartz
Keyword(s):  
Culture Medium ◽  
Medium Ph ◽  
Stripe Pattern ◽  
Line System ◽  
Polar Order ◽  
Visible Part ◽  
Opposite Position ◽  
Stentor Coeruleus ◽  
Pass Through ◽  
Thin Plasma

Treatment of Stentor with very acid culture medium (pH 2.8) results in delamination of the cortex from the endoplasm . This procures the possibility to strip off the cortex with all its acid resistant organelles at minimum loss of endoplasm. Acid sensitive organelles react with dissolution or more or less damage. The endoplast may survive giving rise to a thin plasma membrane, which may be (even several times) shed and renewed. 10.5% of the decorticated cells regenerated a complete cortex. The ball-shaped and apparently apolar endoplast may pass through an ameboid stage. The visible part of the regeneration process begins with the appearance of a chaotic line system , which in regaining the polar order turns out to be the pigment free interstripe spaces. The ubiquitous appearing of foot anlagen underline the apolar situation. Nevertheless, the problem of endoplasmic preservation of polarity requires further, presently running, experiments. Obvious polarity begins to restore with the development of first membranelies and - approximately in opposite position with these - the definitive foot develops with the holdfast organelle. The ordering of stripe pattern starts from the foot and proceeds to the cells apex. With respect to Tartar’s contradicting result (death o fall decorticated cells) the new experiments do not refute Tartars conclusions, since the technique is different from his only micro-surgical method. This will be discussed in a following paper in context with the results of some other experiments. The here described total regeneration of the cortex in Stentor is not consummated as cytotactic differentiation depending on preformed structure.

Influence of Culture Medium pH on Growth, Aggregation, and Biofilm Formation of Xylella fastidiosa

2008 ◽  
Vol 57 (2) ◽  
pp. 127-132 ◽  
Author(s):  
Nelson Arno Wulff ◽  
Anelise Galdino Mariano ◽  
Patrice Gaurivaud ◽  
Leonardo Cesar de Almeida Souza ◽  
Andrea Cassia Diez Virgílio ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Culture Medium ◽  
Xylella Fastidiosa ◽  
Medium Ph

The Influence of pH and Lichen Metabolites (Vulpinic Acid and (+) Usnic Acid) on the Growth of the Lichen Photobiont Trebouxia 1rregularis.

1998 ◽  
Vol 30 (06) ◽  
pp. 577-582 ◽  
Author(s):  
M. Bačkor ◽  
J. Hudá ◽  
M. Repčák ◽  
W. Ziegler§ ◽  
M. Bačkorová
Keyword(s):  
Biomass Production ◽  
Culture Medium ◽  
Usnic Acid ◽  
Concentration Effect ◽  
Medium Ph ◽  
Ph Values ◽  
Lichen Metabolites ◽  
Influence Of Ph ◽  
Quantitative Response

AbstractThe quantitative response to two lichen metabolites, vulpinic acid and (+) usnic acid, on biomass production by the photobiontTrebouxia irregularis, isolated from the lichenCladina mitis, was determined experimentally. The presence of usnic acid resulted in inhibition of photobiont growth, and the effect depended on the pH of the culture medium. The concentration effect of usnic acid was observed. The application of vulpinic acid almost completely inhibited the growth ofT.irregularisand no significant differences were found among samples at different medium pH values.

Flocculating Activity Optimization of Lactobacillus paracasei subsp.paracasei L1 in Corn Liquid

2013 ◽  
Vol 850-851 ◽  
pp. 1172-1175
Author(s):  
Xiao Na Li ◽  
Xin Hua Li ◽  
Li Li Zhang ◽  
Jing Mu
Keyword(s):  
Culture Medium ◽  
Corn Starch ◽  
Culture Conditions ◽  
Lactobacillus Paracasei ◽  
Analysis Method ◽  
Medium Ph ◽  
Inoculum Concentration ◽  
Flocculating Activity ◽  
Initial Ph ◽  
Surface Analysis Method

In order to improve the production process of corn starch and accelerate the separation of corn starch, flocculating activity of lactobacillus paracasei subsp.paracasei L1 isolated from natural fermentation of sweet potato acid liquor in corn liquid was optimized. The main influencing factors were determined by Plackett-Burman experimental design and the flocculating activity was optimized by response surface analysis method. The optimum culture conditions was: inoculum concentration 18%, lactose addition 0.2%, corn steeping time 0h, culture temperature 30°C, initial pH of the culture medium pH 6.5, culture time 36h, yeast extract addition 1%,concentration of corn thick liquid 1:3(w/v).The theoretical value of the flocculating activity was 0.1906 mg/ml and the verified value was 0.1854 mg/ml.

Combination effect of diurnal exposure to sucrose and nocturnal exposure to lactose on enamel demineralization

2021 ◽  
Author(s):  
Juliana Campos Vieira ◽  
Jaime Aparecido Cury ◽  
Antonio Pedro Ricomini Filho
Keyword(s):  
Culture Medium ◽  
Sucrose Solution ◽  
Surface Hardness ◽  
Extracellular Polysaccharides ◽  
Medium Ph ◽  
Lactose Fermentation ◽  
The Media ◽  
Bovine Enamel ◽  
One Way Anova ◽  
Chemical Indicator

We have hypothesized that the association between human milk and caries in breastfeeding children could be explained by the combination of a diurnal cariogenic diet with the nocturnal lactose fermentation, conditions simulated in this experimental study. Cariogenic biofilm was formed on bovine enamel slabs, which were exposed 8x/day for 3 min to a 10% sucrose solution, simulating a highly cariogenic diurnal diet, or 50 mM NaCl solution (control). Simulating the nocturnal retention of milk in mouth, biofilms were transferred to culture medium containing 0.7% lactose for 2 h, or only to culture medium (control). Four groups were designed (n=12): Ctrl, no exposure to diurnal sucrose or nocturnal lactose; Lac, only nocturnal exposure to lactose (2 h); Suc, only diurnal exposure to sucrose (8x/day); and Suc→Lac, diurnal exposure to sucrose (8x/day) followed by nocturnal exposure to lactose (2 h). The medium was changed 3x/day, at the beginning of the day, and after diurnal and nocturnal exposures. Calcium in the medium was determined as chemical indicator of partial demineralizations occurred during the diurnal and the nocturnal treatments; the medium pH was also determined. After 96 h of growth, biofilms were harvested to evaluate CFU, biomass, and extracellular polysaccharides, soluble and insoluble. The percentage of enamel surface hardness loss (%SHL) was evaluated as cumulative demineralization. Data were analyzed by one-way ANOVA, Tukey’s test (α=5%). Highest %SHL (p<0.05) was found for Suc→Lac (40.6%) group when compared to Suc (32.1%), Lac (7.7%), and Ctrl (3.8%). Calcium released during the diurnal and nocturnal treatments were respectively: Suc→Lac=Suc>Lac=Ctrl and Suc→Lac=Lac>Suc=Ctrl (p<0.05). Regarding Ctr group, calcium released from nocturnal lactose fermentation by Suc→Lac group was 4-fold greater than that provoked by Lac group. The findings were supported by the pH of the media. The data suggest that the biofilm formed under diurnal exposure to sucrose enhances the cariogenicity of nocturnal exposure to lactose.

Changes in culture medium pH by cell suspension cultures of Dioscorea deltoidea

1984 ◽  
Vol 35 (3) ◽  
pp. 207-212 ◽  
Author(s):  
R.G. Butenko ◽  
A.Kh. Lipsky ◽  
N.D. Chernyak ◽  
H.C. Arya
Keyword(s):  
Cell Suspension ◽  
Culture Medium ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Medium Ph ◽  
Dioscorea Deltoidea

scholarly journals PROMOCIÓN DEL CRECIMIENTO DE Baccharis macrantha (ASTERACEAE) CON BACTERIAS SOLUBILIZADORAS DE FOSFATOS ASOCIADAS A SU RIZOSFERA

2015 ◽  
Vol 20 (3) ◽  
pp. 121-131
Author(s):  
Luz Marina LIZARAZO FORERO ◽  
Elsa Giovanna ÁVILA MARTÍNEZ ◽  
Francisco CORTÉS PÉREZ
Keyword(s):  
Culture Medium ◽  
Heterotrophic Bacteria ◽  
Growth Promotion ◽  
Dry Weight ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Firmus ◽  
Trypticase Soya Agar ◽  
Phosphate Solubilizing ◽  
Solubilizing Capacity

<p>El objetivo de esta investigación fue aislar y caracterizar bacterias solubilizadoras de fosfatos (BSF) asociadas a la rizosfera de <em>Baccharis macrantha </em>y <em>Viburnum triphyllum,</em> y evaluar su capacidad para solubilizar fosfatos en condiciones <em>in vitro</em>. Además se determinó el efecto de la inoculaciónde las cepas de BSF más eficientes sobre el crecimiento de <em>B. macrantha</em>. Las muestras de suelo rizosférico de <em>B. macrantha </em>y <em>V. triphyllum </em>fueron colectadas en los meses de mayo-período de lluvia y septiembre-período seco del 2012. Para la cuantificación de bacterias heterótrofas cultivables y BSF se empleó el método de recuento en placa en los medios Agar Tripticasa de Soya y Pikovskaya (PVK) respectivamente. La capacidad de solubilización de fosfatos de las cepas aisladas se estimó a partir del diámetro de los halos formados alrededor de las colonias en el medio de cultivo PVK después de 7 días de incubación a 28 °C. Los ensayos de inoculación en <em>B. macrantha </em>se realizaron con las BSF más eficientes<em>. </em>La inoculación de las BSF <em>B. firmus y P. fluorescens</em> de forma individual y como inoculante combinado mostro un efecto benéfico, incrementando significativamente el porcentaje de germinación de semillas, la altura de la plántula, la longitud de la raíz y el peso seco de <em>B. macrantha</em>. La inoculación de BSF podría ser considerada una estrategia para mejorar el crecimiento y establecimiento de <em>B. macrantha</em> en pastizales abandonados.</p><p><strong>Growth Promotion of <em>Baccharis macrantha </em>(Asteraceae) by Phosphate Solubilizing Rhizosphere Bacteria</strong>     </p><p>The objectives of this research was to isolate and characterize phosphate solubilizing bacteria (BSF) associated to the rhizosphere of <em>Baccharis macrantha</em> and <em>Viburnum triphyllum</em>, and to assess their ability to solubilize phosphate under conditions in vitro. Furthermore to determine the effect of inoculation of the strains BSF more efficient on the growth of <em>B. macrantha</em>. Rhizosphere soil samples of <em>B. macrantha</em> and <em>V. triphyllum </em>were collected in the months of May-rainy season and September-period dry the 2012. Trypticase Soya Agar and Pikovskaya (PVK) were used for quantification of culturable heterotrophic bacteria and BSF, respectively. The phosphate solubilizing capacity of the isolated strains was estimated from the diameter of the halo around the colonies formed in the culture medium PVK after 7 days incubation at 28 °C. Inoculation assays were performed with more efficient BSF in <em>B. macrantha. </em>Inoculation of BSF <em>Bacillus firmus</em> and <em>Pseudomona fluorescens </em>individually and as inoculant combined showed a beneficial effect, significantly increasing the percentage of seed germination, seedling height, root length and dry weight of <em>B . macrantha</em>. Inoculation the BSF could be considered a strategy to improve the growth and development of <em>B. macrantha</em> in abandoned pastures</p>

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