Real-Time Monitoring of Anthocyanidin-Zeolite Complex Exposed to Skin Cells

2013 ◽  
Vol 339 ◽  
pp. 742-747
Author(s):  
Aroonsri Priprem ◽  
Supatra Porasuphatana ◽  
Sakolthep Srirak ◽  
Wiboon Pantu ◽  
Teerasak Damrongrungrueng ◽  
...  
Keyword(s):  
Real Time ◽  
Langmuir Adsorption Isotherm ◽  
Cell Analysis ◽  
Zeolite A ◽  
Mesoporous Zeolite ◽  
Clitoria Ternatea ◽  
Langmuir Adsorption ◽  
Skin Cells ◽  
Household Products ◽  
Human Epidermoid Carcinoma

Effect of an anthocyanidin-zeolite complex (A-Z) was investigated by real-time cell analysis in cultured human epidermoid carcinoma keratinocytes (A431) and human forehead fibroblasts (HFF). A mesoporous zeolite, a potential adsorbent applied in household products, was complexed with an anthocyanidin, extracted from blue flowers ofClitoria ternateaLinn. The A-Z was linearly correlated within a range of 0.05 0.3 mg/ml of the anthocyanidin, analyzed by HPLC, in accordance to Langmuir adsorption isotherm (r= 0.992,p> 0.05). In comparison to zeolite or anthocyanidin alone, A431 and HFF showed significantly cell survival with improved IC50upon exposure to A-Z (p< 0.05). The adsorbed anthocyanidin onto the zeolite reduced the toxic potentials of each.

scholarly journals Transformation of Glass Fiber Waste into Mesoporous Zeolite-Like Nanomaterials with Efficient Adsorption of Methylene Blue

2021 ◽  
Vol 13 (11) ◽  
pp. 6207
Author(s):  
Cheng-Kuo Tsai ◽  
Jao-Jia Horng
Keyword(s):  
Methylene Blue ◽  
Glass Fiber ◽  
Environmental Concern ◽  
Cost Effective ◽  
Environmentally Benign ◽  
Mesoporous Zeolite ◽  
Trace Metal Ions ◽  
Langmuir Adsorption ◽  
Ceramic Ring ◽  
Cost Effective Method

Recycling and reusing glass fiber waste (GFW) has become an environmental concern, as the means of disposal are becoming limited as GFW production increases. Therefore, this study developed a novel, cost-effective method to turn GFW into a mesoporous zeolite-like nanomaterial (MZN) that could serve as an environmentally benign adsorbent and efficient remover of methylene blue (MB) from solutions. Using the Taguchi optimizing approach to hydrothermal alkaline activation, we produced analcime with interconnected nanopores of about 11.7 nm. This MZN had a surface area of 166 m2 g−1 and was negatively charged with functional groups that could adsorb MB ranging from pH 2 to 10 and all with excellent capacity at pH 6.0 of the maximum Langmuir adsorption capacity of 132 mg g−1. Moreover, the MZN adsorbed MB exothermically, and the reaction is reversible according to its thermodynamic parameters. In sum, this study indicated that MZN recycled from glass fiber waste is a novel, environmentally friendly means to adsorb cation methylene blue (MB), thus opening a gateway to the design and fabrication of ceramic-zeolite and tourmaline-ceramic balls and ceramic ring-filter media products. In addition, it has environmental applications such as removing cation dyes and trace metal ions from aqueous solutions and recycling water.

scholarly journals Diffusion of calcium ions in retinal rods. A theoretical calculation.

1981 ◽  
Vol 77 (4) ◽  
pp. 475-487 ◽  
Author(s):  
S McLaughlin ◽  
J Brown
Keyword(s):  
Diffusion Coefficient ◽  
Adsorption Isotherm ◽  
Calcium Ions ◽  
Outer Segment ◽  
Effective Diffusion ◽  
Langmuir Adsorption Isotherm ◽  
Diffuse Double Layer ◽  
Langmuir Adsorption ◽  
Retinal Rods ◽  
Disk Membrane

The Fick diffusion equation is combined with the Langmuir adsorption isotherm and the relevant equations from the Gouy-Chapman theory of the electrical diffuse double layer to demonstrate that the effective diffusion coefficient of calcium ions, both in the cytoplasm of the rod outer segment and within the aqueous space bounded by the disk membrane, should be reduced by a factor of 10-100 because these ions adsorb to phospholipids present in the disk membrane.

A further note on the measurement of the affinity of a dye (Azure A) for histological substrates

1965 ◽  
Vol s3-106 (76) ◽  
pp. 299-306
Author(s):  
D. J. GOLDSTEIN
Keyword(s):  
Adsorption Isotherm ◽  
Goblet Cell ◽  
Binding Sites ◽  
Langmuir Isotherm ◽  
Langmuir Adsorption Isotherm ◽  
Intestinal Epithelial ◽  
Langmuir Adsorption ◽  
Azure A ◽  
First Approximation ◽  
The Ideal

If two histological sections, of thickness nµ and 2nµ, and having the same apparent intensity of staining, are at equilibrium with dyebaths of concentration B1 and B2 respectively, the affinity of the histological substrate for the dye is, under denned conditions, given by the expression ΔF° = - RT In I/B, where B is either (i) B2 if the thinner section is stained to saturation, or (ii) B1 if B1 = 3B2. The affinity tends to be greater when measured in a weaker dyebath, as implied in method (ii). Evidence is presented suggesting that the uptake of the basic dye Azure A by pancreatic basal chromidial substance, goblet cell mucin and intestinal epithelial cytoplasm follows a Langmuir adsorption isotherm to a first approximation, in that, at low dyebath concentrations, the uptake of dye is proportional to the concentration of dyebath with which the substrate is in equilibrium, while in strong dyebaths the uptake approaches a plateau. A deviation from the ideal Langmuir isotherm in dyebaths of moderate concentration, observed in epithelial cytoplasm and to a lesser extent in pancreatic basal chromidial substance, may have been due to interaction between neighbouring dye-binding sites, or to the presence in a single area of sites with different affinities for the dye.

THE SORPTION OF ACIDS BY WOOL

1949 ◽  
Vol 27b (12) ◽  
pp. 879-889 ◽  
Author(s):  
R. Donovan ◽  
P. Larose
Keyword(s):  
Hydrochloric Acid ◽  
Adsorption Isotherm ◽  
Sulphuric Acid ◽  
Sodium Sulphate ◽  
Langmuir Adsorption Isotherm ◽  
Langmuir Equation ◽  
Recent Application ◽  
Satisfactory Explanation ◽  
Isotherm Equation ◽  
Langmuir Adsorption

The amount of acid sorbed by wool from solutions of sulphuric acid of four different strengths (namely, 0.0505, 0.0339, 0.0182, and 0.0101 molar) and containing sodium sulphate in amounts varying up to 0.16 molar has been determined. It has been found that the presence of the salt has little effect on the quantity of acid sorbed within those limits. The results are analyzed in the light of the theory of Gilbert and Rideal but this theory fails to give a satisfactory explanation of the results obtained. It is possible, however, to explain the results of the authors' experiments on the basis of the recent application of the Donnan equilibrium by Peters and Speakman. The Langmuir adsorption isotherm equation has been applied to data on the absorption of hydrochloric acid and of sulphuric acid by wool. The data appear to fit the Langmuir equation and give, for the maximum combining capacity, values that agree well with those estimated in other ways.

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