A Study on Bacterioplankton Community Composition in a Nature Reservoir

2013 ◽  
Vol 361-363 ◽  
pp. 747-750
Author(s):  
Bing Bing Liu ◽  
Hua Yong Zhang ◽  
Song Bo Cui
Keyword(s):  
Community Composition ◽  
Clustering Analysis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Small Scale ◽  
Bacterioplankton Community ◽  
Gradient Gel Electrophoresis ◽  
Polymerase Chain ◽  
The Relationship ◽  
Total Species ◽  
Plankton Communities

The relationship between bacterioplankton community composition and environmental factors in a constructed small-scale reservoir was studied. Polymerase chain reaction (PCR) - denaturing gradient gel electrophoresis (DGGE) fingerprinting was used to detect plankton communities. Species composition of the sites was heterogeneous; 60 % of the total species were restricted to single samples and only 2 bands were found in all investigated samples. Clustering analysis illustrated a strong correlation between samples taken from same sites. Canonical correspondence analysis (CCA) results showed that bacterioplankton community composition was primarily correlated with NO3--N and CODCr. Bacterioplankton community composition could reflect the environmental conditions to some extent.

scholarly journals Macrophyte Species Drive the Variation of Bacterioplankton Community Composition in a Shallow Freshwater Lake

2011 ◽  
Vol 78 (1) ◽  
pp. 177-184 ◽  
Author(s):  
Jin Zeng ◽  
Yuanqi Bian ◽  
Peng Xing ◽  
Qinglong L. Wu
Keyword(s):  
Community Composition ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Single Species ◽  
Freshwater Lake ◽  
Clone Libraries ◽  
Bacterioplankton Community ◽  
Macrophyte Species ◽  
Gradient Gel Electrophoresis ◽  
Microcosm Studies ◽  
Species Specific

ABSTRACTMacrophytes play an important role in structuring aquatic ecosystems. In this study, we explored whether macrophyte species are involved in determining the bacterioplankton community composition (BCC) in shallow freshwater lakes. The BCC in field areas dominated by different macrophyte species in Taihu Lake, a large, shallow freshwater lake, was investigated over a 1-year period. Subsequently, microcosm experiments were conducted to determine if single species of different types of macrophytes in an isolated environment would alter the BCC. Denaturing gradient gel electrophoresis (DGGE), followed by cloning and sequence analysis of selected samples, was employed to analyze the BCC. The DGGE results of the field investigations indicated that the BCC changed significantly from season to season and that the presence of different macrophyte species resulted in lower BCC similarities in the summer and fall. LIBSHUFF analysis of selected clone libraries from the summer demonstrated different BCCs in the water column surrounding different macrophytes. Relative to the field observations, the microcosm studies indicated that the BCC differed more pronouncedly when associated with different species of macrophytes, which was also supported by LIBSHUFF analysis of the selected clone libraries. Overall, this study suggested that macrophyte species might be an important factor in determining the composition of bacterial communities in this shallow freshwater lake and that the species-specific influence of macrophytes on BCC is variable with the season and distance.

scholarly journals Investigations on the Effects of Dietary Essential Oils and Different Husbandry Conditions on the Gut Ecology in Piglets after Weaning

2009 ◽  
Vol 2009 ◽  
pp. 1-9 ◽  
Author(s):  
P. Janczyk ◽  
R. Pieper ◽  
V. Urubschurov ◽  
K. R. Wendler ◽  
W. B. Souffrant
Keyword(s):  
Essential Oils ◽  
Dry Matter ◽  
Distal Part ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Control Diet ◽  
Short Chain Fatty Acids ◽  
Growth Promoters ◽  
Gradient Gel Electrophoresis ◽  
Antibiotic Growth Promoters ◽  
Polymerase Chain

Essential oils (EO) are being considered as possible alternatives to in-feed antibiotic growth promoters in pig nutrition. The effects of an EO mixture consisting of limonene, eugenol and pinene (10.0, 2.0, and 4.8 mg/kg diet, resp.) on gut physiology and ecology were studied in piglets. The experiment was conducted at low (commercial farm) and high hygienic conditions (experimental farm), to elucidate interactions between EO supplementation and husbandry methods. Piglets were weaned at 28 days of age, when they were offered either a control diet (C) or C with EO. Four piglets were sacrificed in each group on day 29, 30, 33 and 39. Digesta from the third distal part of the small intestine and from the colon were sampled and analysed for pH, dry matter, lactic acid, short chain fatty acids and ammonia concentrations. Enterobacteria, enterococci, lactobacilli and yeast counts were obtained by plating. Genomic DNA was extracted from digesta and polymerase chain reaction—denaturing gradient gel electrophoresis was performed. Individual microbial communities were identified at each farm. Age affected the intestinal parameters. No effects of the EO with exception for a significant reduction in colon bacterial diversity at 39 days of age could be recorded at experimental farm.

The assessment of the coke wastewater treatment efficacy in rotating biological contractor

2015 ◽  
Vol 73 (5) ◽  
pp. 1202-1210 ◽  
Author(s):  
G. Cema ◽  
S. Żabczyński ◽  
A. Ziembińska-Buczyńska
Keyword(s):  
Biological Treatment ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Rotating Biological Contactor ◽  
Coke Wastewater ◽  
Promising Tool ◽  
Start Up ◽  
Gradient Gel Electrophoresis ◽  
Real Wastewater ◽  
Polymerase Chain ◽  
Anammox Process

Coke wastewater is known to be relatively difficult for biological treatment. Nonetheless, biofilm-based systems seem to be promising tool for such treatment. That is why a rotating biological contactor (RBC) system focused on the Anammox process was used in this study. The experiment was divided into two parts with synthetic and then real wastewater. It was proven that it is possible to treat coke wastewater with RBC but such a procedure requires a very long start-up period for the nitritation (190 days), as well as for the Anammox process, where stable nitrogen removal over 70% was achieved after 400 days of experiment. Interestingly, it was possible at a relatively low (20.2 ± 2.2 °C) temperature. The polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE) based monitoring of the bacterial community showed that its biodiversity decreased when the real wastewater was treated and it was composed mainly of GC-rich genotypes, probably because of the modeling influence of this wastewater and the genotypes specialization.

Genetic diversity of picoeukaryotes in eight lakes differing in trophic status

2011 ◽  
Vol 57 (2) ◽  
pp. 115-126 ◽  
Author(s):  
Biying Zhao ◽  
Meijun Chen ◽  
Ying Sun ◽  
Jiaxin Yang ◽  
Feizhou Chen
Keyword(s):  
Genetic Diversity ◽  
Community Composition ◽  
Trophic Status ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Oligotrophic Lake ◽  
Rrna Genes ◽  
Multivariate Statistical ◽  
Mesotrophic Lake ◽  
Gradient Gel Electrophoresis ◽  
Lake Xuanwu

The genetic diversity of picoeukaryotes (0.2–5.0 µm) was investigated in 8 lakes differing in trophic status in Nanjing, China. Denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing of 18S rRNA genes were applied to analyze the picoeukaryotic communities. DGGE analysis showed that among the 8 lakes, the diversity of picoeukaryotes was greatest in the mesotrophic Lake Nan (24 bands) and least in the oligotrophic Lake Qian (12 bands). Cluster analysis of DGGE profiles revealed that the 8 lakes were grouped into 2 distinct clusters. Cluster 1 contained lakes Mochou, Zixia, Huashen, Nan, Pipa, and Qian, while cluster 2 contained lakes Xuanwu and Baijia. Clone libraries were constructed from the mesotrophic Lake Xuanwu and the oligotrophic Lake Zixia, and the 2 libraries were compared using the program LIBSHUFF. This analysis indicated that the picoeukaryotic community composition differed significantly between the 2 lakes (p = 0.001). A total of 25 operational taxonomic units were detected; 18 (62 clones) were related to known eukaryotic groups, while 7 (30 clones) were not affiliated with any known eukaryotic group. Alveolates and stramenopiles were the dominant groups in Lake Xuanwu, while alveolates and chlorophyta predominated in Lake Zixia. Multivariate statistical analysis indicated that the differences in the picoeukaryotic community composition of the 8 lakes might be related to trophic status and top-down regulation by metazooplankton.

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