Rapid HPLC of Cyanidin and Delphinidin of an Anthocyanin Complex Exposed to Human Gingival Epithelial Cells

A rapid isocratic HPLC was developed and validated for use in simultaneous analysis of cyanidin and delphinidin extracted from purple cobs of Zea mays L. ceritina Kulesh. (CC), blue petals of Clitoria ternatea L. (CT) and an anthocyanin complex (AC). The method was shown to be rapid, precise and accurate within 5 20 μg/ml (r > 0.997) with limits of detection and quantitation of 0.45 and 1.52 μg/ml for cyanidin and 4.04 and 13.3 μg/ml for delphinidin, respectively. It could quantitatively detect and compare changes in cyanidin and delphinidin from the AC exposed to human gingival epithelium cells.

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Stimulation of Mesenchymal-epithelial Transition Factor (c-Met) Expression in Candida Albicans-infected Human Gingival Epithelial Cells and Rat Gingival Epithelium

International Journal of Oral-Medical Sciences ◽

10.5466/ijoms.10.229 ◽

2012 ◽

Vol 10 (4) ◽

pp. 229-234 ◽

Cited By ~ 1

Author(s):

Jotaro Ohmori ◽

Tonami Ikuta ◽

Noboru Kuboyama ◽

Yoshimitsu Abiko

Keyword(s):

Candida Albicans ◽

Epithelial Cells ◽

Factor I ◽

Gingival Epithelial Cells ◽

Gingival Epithelium ◽

Mesenchymal Epithelial Transition Factor ◽

Human Gingival Epithelial Cells ◽

Mesenchymal Epithelial Transition ◽

Stimulation Of

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The Ultrastructure of Monkey Gingival Epithelium

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010006009x ◽

1967 ◽

Vol 25 ◽

pp. 16-17

Author(s):

C.N. Sun

Keyword(s):

Epithelial Cells ◽

Macaca Nemestrina ◽

Stratum Granulosum ◽

Solution Ph ◽

Gingival Epithelial Cells ◽

Stratum Spinosum ◽

Cell Layers ◽

Gingival Epithelium ◽

The Individual ◽

Different Thickness

The present study demonstrates the ultrastructure of the gingival epithelium of the pig tail monkey (Macaca nemestrina). Specimens were taken from lingual and facial gingival surfaces and fixed in Dalton's chrome osmium solution (pH 7.6) for 1 hr, dehydrated, and then embedded in Epon 812.Tonofibrils are variable in number and structure according to the different region or location of the gingival epithelial cells, the main orientation of which is parallel to the long axis of the cells. The cytoplasm of the basal epithelial cells contains a great number of tonofilaments and numerous mitochondria. The basement membrane is 300 to 400 A thick. In the cells of stratum spinosum, the tonofibrils are densely packed and increased in number (fig. 1 and 3). They seem to take on a somewhat concentric arrangement around the nucleus. The filaments may occur scattered as thin fibrils in the cytoplasm or they may be arranged in bundles of different thickness. The filaments have a diameter about 50 A. In the stratum granulosum, the cells gradually become flatted, the tonofibrils are usually thin, and the individual tonofilaments are clearly distinguishable (fig. 2). The mitochondria and endoplasmic reticulum are seldom seen in these superficial cell layers.

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Cigarette Smoke Stimulates SARS-CoV-2 Internalization by Activating AhR and Increasing ACE2 Expression in Human Gingival Epithelial Cells

International Journal of Molecular Sciences ◽

10.3390/ijms22147669 ◽

2021 ◽

Vol 22 (14) ◽

pp. 7669

Author(s):

Cassio Luiz Coutinho Almeida-da-Silva ◽

Harmony Matshik Dakafay ◽

Kaitlyn Liu ◽

David M. Ojcius

Keyword(s):

Epithelial Cells ◽

Oral Cavity ◽

Cigarette Smoke ◽

Large Body ◽

Receptor Expression ◽

Host Cells ◽

Dependent Manner ◽

Gingival Epithelial Cells ◽

Human Gingival Epithelial Cells ◽

Oral Cells

A large body of evidence shows the harmful effects of cigarette smoke to oral and systemic health. More recently, a link between smoking and susceptibility to coronavirus disease 2019 (COVID-19) was proposed. COVID-19 is due to infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which uses the receptor ACE2 and the protease TMPRSS2 for entry into host cells, thereby infecting cells of the respiratory tract and the oral cavity. Here, we examined the effects of cigarette smoke on the expression of SARS-CoV-2 receptors and infection in human gingival epithelial cells (GECs). We found that cigarette smoke condensates (CSC) upregulated ACE2 and TMPRSS2 expression in GECs, and that CSC activated aryl hydrocarbon receptor (AhR) signaling in the oral cells. ACE2 was known to mediate SARS-CoV-2 internalization, and we demonstrate that CSC treatment potentiated the internalization of SARS-CoV-2 pseudovirus in GECs in an AhR-dependent manner. AhR depletion using small interference RNA decreased SARS-CoV-2 pseudovirus internalization in CSC-treated GECs compared with control GECs. Our study reveals that cigarette smoke upregulates SARS-CoV-2 receptor expression and infection in oral cells. Understanding the mechanisms involved in SARS-CoV-2 infection in cells of the oral cavity may suggest therapeutic interventions for preventing viral infection and transmission.

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Pro-inflammatory cytokine responses in human gingival epithelial cells after stimulation with cell wall extract of Aggregatibacter actinomycetemcomitans subtypes

Anaerobe ◽

10.1016/j.anaerobe.2017.08.001 ◽

2017 ◽

Vol 48 ◽

pp. 103-109 ◽

Cited By ~ 5

Author(s):

Nuntiya Pahumunto ◽

Pareena Chotjumlong ◽

Anupong Makeudom ◽

Suttichai Krisanaprakornkit ◽

Gunnar Dahlen ◽

...

Keyword(s):

Cell Wall ◽

Epithelial Cells ◽

Inflammatory Cytokine ◽

Aggregatibacter Actinomycetemcomitans ◽

Cytokine Responses ◽

Gingival Epithelial Cells ◽

Cell Wall Extract ◽

Human Gingival Epithelial Cells

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Identification of Porphyromonas gingivalis Genes Specifically Expressed in Human Gingival Epithelial Cells by Using Differential Display Reverse Transcription-PCR

Infection and Immunity ◽

10.1128/iai.72.7.3752-3758.2004 ◽

2004 ◽

Vol 72 (7) ◽

pp. 3752-3758 ◽

Cited By ~ 34

Author(s):

Yoonsuk Park ◽

Özlem Yilmaz ◽

Il-Young Jung ◽

Richard J. Lamont

Keyword(s):

Epithelial Cells ◽

Porphyromonas Gingivalis ◽

Abc Transporter ◽

Differential Display ◽

Molecular Mechanisms ◽

Gene Products ◽

Reverse Transcription Pcr ◽

Gingival Epithelial Cells ◽

Insertional Inactivation ◽

Human Gingival Epithelial Cells

ABSTRACT Porphyromonas gingivalis, one of the causative agents of adult periodontitis, can invade and survive within host epithelial cells. The molecular mechanisms by which P. gingivalis induces uptake and adapts to an intracellular environment are not fully understood. In this study, we have investigated the genetic responses of P. gingivalis internalized within human gingival epithelial cells (GECs) in order to identify factors involved in invasion and survival. We compared the differential display of arbitrarily PCR-amplified gene transcripts in P. gingivalis recovered from GECs with the display of transcripts in P. gingivalis control cultures. Over 20 potential differentially expressed transcripts were identified. Among these, pepO, encoding an endopeptidase, and genes encoding an ATP-binding cassette (ABC) transporter and a cation-transporting ATPase were upregulated in GECs. To investigate the functionality of these gene products, mutants were generated by insertional inactivation. Compared to the parental strain, mutants of each gene showed a significant reduction in their invasion capabilities. In addition, GEC cytoskeletal responses to the mutants were distinct from those induced by the parent. In contrast, adhesion of the mutant strains to GECs was not affected by lack of expression of the gene products. These results suggest that PepO, a cation-transporting ATPase, and an ABC transporter are required for the intracellular lifestyle of P. gingivalis.

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