Preparation and Characterization of Spidroin and PLLA Blended Nanofiber Mats

2011 ◽  
Vol 175-176 ◽  
pp. 127-131 ◽  
Author(s):  
Min Zhang ◽  
Ye Mei Zhang ◽  
Wei Wu ◽  
Ai Li Zhang ◽  
Zhi Juan Pan
Keyword(s):  
Spider Silk ◽  
Molecular Conformation ◽  
Random Coil ◽  
Composite Fibers ◽  
Fiber Mats ◽  
Initial Modulus ◽  
Plla Fiber ◽  
Α Helix ◽  
Nanofiber Mats

Due to the exceptional biocompatibility of spider silk and poly-L-lactic acid (PLLA), electrospun PLLA or spidroin fiber mat is one of the best biomaterials. The diameter of electropun PLLA fiber decreased and mechanical property of such fiber mats was improved once some spidroin was added into PLLA/Hexaflorisopropanol (HFIP) solution. The influence of electrospinning voltage and distance on the shape and mechanical properties of spidroin/PLLA composite fibers was investigated as well as the molecular conformation and crystalline structure of the electrospun fibers. The results revealed that the diameter of spidroin/PLLA fiber was non-uniform, which varied from 300nm to 1000nm. The content of random coil or α-helix structure was about 45%, and spidroin presented a crystal structure separated with PLLA in their composite fibers. With the raise of voltage, the proportion of nano-sized fibers in an electrospun spidroin/PLLA fiber mat increased and tended to be unchanged. The breaking strength of the mat was improved following the increase of voltage. Meanwhile, the ratio of nano-sized fibers and initial modulus of the spidroin/PLLA fiber mat underwent the convex change against the electrospinning distance between the needle and collector.

Influence of Post-Treatment with Methanol Vapor on the Properties of SF/P(LLA-CL) Nanofibrous Scaffolds

2011 ◽  
Vol 236-238 ◽  
pp. 2221-2224
Author(s):  
Kui Hua Zhang ◽  
Xiu Mei Mo
Keyword(s):  
Electrospun Nanofibers ◽  
Random Coil ◽  
Methanol Vapor ◽  
Nanofibrous Scaffolds ◽  
Nanofibrous Structure ◽  
Α Helix ◽  
Β Sheet ◽  
Ideal Method ◽  
C Nmr

In order to improve water-resistant ability silk fibroin (SF) and SF/P(LLA-CL) blended nanofibrous scaffolds for tissue engineering applications, methanol vapor were used to treat electrospun nanofibers. SEM indicated SF and SF/ P(LLA-CL) scaffolds maintained nanofibrous structure after treated with methanol vapor and possessed good water-resistant ability. Characterization of 13C NMR clarified methanol vapor induced SF conformation from random coil or α- helix to β-sheet. Moreover, treated SF/ P (LLA-CL) nanofibrous scaffolds still kept good mechanical properties. Methanol vapor could be ideal method to treat SF and SF/ P(LLA-CL) nanofibrous scaffolds for biomedical applications.

scholarly journals Fabrication and Characterization of Lignin/Dendrimer Electrospun Blended Fiber Mats

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 518
Author(s):  
Somaye Akbari ◽  
Addie Bahi ◽  
Ali Farahani ◽  
Abbas S. Milani ◽  
Frank Ko
Keyword(s):  
Thermal Characteristics ◽  
Dendritic Polymers ◽  
Amino Groups ◽  
Fiber Mats ◽  
Potential Applications ◽  
Solution Electrospinning ◽  
Nanofiber Mats ◽  
Softwood Kraft Lignin ◽  
First Time

Blending lignin as the second most abundant polymer in Nature with nanostructured compounds such as dendritic polymers can not only add value to lignin, but also increase its application in various fields. In this study, softwood Kraft lignin/polyamidoamine dendritic polymer (PAMAM) blends were fabricated by the solution electrospinning to produce bead-free nanofiber mats for the first time. The mats were characterized through scanning electron microscopy, Fourier transform infrared (FTIR) spectroscopy, zeta potential, and thermogravimetry analyses. The chemical intermolecular interactions between the lignin functional groups and abundant amino groups in the PAMAM were verified by FTIR and viscosity measurements. These interactions proved to enhance the mechanical and thermal characteristics of the lignin/PAMAM mats, suggesting their potential applications e.g. in membranes, filtration, controlled release drug delivery, among others.

A facile method to prepare CdS/polymer nanocomposite fibers for the photodegradation of methylene blue under sunlight

2017 ◽  
Vol 37 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Xinjian Cheng ◽  
Yiru Jia ◽  
Jin Qiang ◽  
Lei Wen ◽  
Chenlong Zhang ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Polymer Composite ◽  
High Efficiency ◽  
Cadmium Acetate ◽  
Polymer Nanocomposite ◽  
Composite Fiber ◽  
Composite Fibers ◽  
Fiber Mats ◽  
Visible Spectra ◽  
Nanofiber Mats

Abstract Nanosized CdS/polymer composite fiber mats were fabricated via a facile electrospinning processing, and the as-prepared composite fiber mats showed excellent photodegradation ability to methylene blue (MB). Polymers bearing N and S atoms with affinity to Cd2+ ions were first synthesized and then used to prepare CdS/polymer composite fibers. In this work, coaxial electrospinning was employed to achieve the preparation of the desired composite fibers using special polymers as core and cadmium acetate [Cd(CH3COO)2] as shell. Then, fiber mats were immersed in thioacetamide (TAA) solution for several minutes, and CdS/polymer composite mats were obtained eventually. The photodegradation catalytic ability was tested using MB as a model dye under sunlight. The photocatalytic efficiency of the CdS/polymer composite fiber mat was recorded and studied by UV-visible spectra, and it showed high efficiency. Nanofiber mats will find real applications in the treatment of wastewater.

scholarly journals Permeability of Electrospun Superhydrophobic Nanofiber Mats

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Sarfaraz U. Patel ◽  
Gabriel M. Manzo ◽  
Shagufta U. Patel ◽  
Prashant S. Kulkarni ◽  
George G. Chase
Keyword(s):  
Fiber Diameter ◽  
Contact Angle Hysteresis ◽  
Contact Angles ◽  
Diameter Distribution ◽  
Fibrous Media ◽  
Water Contact ◽  
Fiber Mats ◽  
Nonwoven Fiber ◽  
Nanofiber Mats

This paper discusses the fabrication and characterization of electrospun nanofiber mats made up of poly(4-methyl-1-pentene) polymer. The polymer was electrospun in different weight concentrations. The mats were characterized by their basis weight, fiber diameter distribution, contact angles, contact angle hysteresis, and air permeability. All of the electrospun nonwoven fiber mats had water contact angles greater than 150 degrees making them superhydrophobic. The permeabilities of the mats were empirically fitted to the mat basis weight by a linear relation. The experimentally measured air permeabilities were significantly larger than the permeabilities predicted by the Kuwabara model for fibrous media.

scholarly journals Imaging of Oral SCC Cells by Raman Micro-Spectroscopy Technique

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3640
Author(s):  
Hidetaka Kinoshita ◽  
Norio Miyoshi ◽  
Toshiyuki Ogasawara
Keyword(s):  
Nucleic Acids ◽  
Molecular Conformation ◽  
Molecular Structures ◽  
Random Coil ◽  
Malignant Tissue ◽  
Spectroscopy Technique ◽  
Cell Level ◽  
Frozen Tissue ◽  
Α Helix ◽  
Β Sheet

We used Raman micro-spectroscopy technique to analyze the molecular changes associated with oral squamous cell carcinoma (SCC) cells in the form of frozen tissue. Previously, Raman micro-spectroscopy technique on human tissue was mainly based on spectral analysis, but we worked on imaging of molecular structure. In this study, we evaluated the distribution of four components at the cell level (about 10 μm) to describe the changes in protein and molecular structures of protein belonging to malignant tissue. We analyzed ten oral SCC samples of five patients without special pretreatments of the use of formaldehyde. We obtained cell level images of the oral SCC cells at various components (peak at 935 cm−1: proline and valine, 1004 cm−1: phenylalanine, 1223 cm−1: nucleic acids, and 1650 cm−1: amide I). These mapping images of SCC cells showed the distribution of nucleic acids in the nuclear areas; meanwhile, proline and valine, phenylalanine, and amide I were detected in the cytoplasm areas of the SCC cells. Furthermore, the peak of amide I in the cancer area shifts to the higher wavenumber side, which indicates the α-helix component may decrease in its relative amounts of protein in the β-sheet or random coil conformation. Imaging of SCC cells with Raman micro-spectroscopy technique indicated that such a new observation of cancer cells is useful for analyzing the detailed distribution of various molecular conformation within SCC cells.

Effect of Different Post-Treatment on the Morphology, Structure and Property of Electrospun SF Nanofiber Mats

2012 ◽  
Vol 557-559 ◽  
pp. 607-610
Author(s):  
Tao Liu ◽  
Feng Zhang ◽  
Bao Qi Zuo
Keyword(s):  
Water Resistance ◽  
Post Treatment ◽  
Treatment System ◽  
Random Coil ◽  
Structure And Property ◽  
Before And After ◽  
Α Helix ◽  
Nanofiber Mats ◽  
Β Sheet ◽  
Conformation Transition

In this paper, we employed Methanol, 75% Ethanol, 100%Ethanol, and Isopropanol to treat electrospun SF nanofiber mats, and studied the morphology, structure and property of SF nanofiber before and after treatment by means of SEM, and FTIR. While the as-spun SF nanofiber mats consisted of α-helix and random coil, post-treatment of the SF nanofiber resulted in significant increase in β-sheet component and improvement of water resistance of the SF nanofiber mats. This effect was not obvious affected when different post-treatment system was used, but methanol and 75% was more effective in inducing conformation transition from α-helix and random coil to β-sheet.

13C CP/MAS NMR Spectroscopic Characterization of Native Silk Fibers

2011 ◽  
Vol 175-176 ◽  
pp. 328-332 ◽  
Author(s):  
Wei Zhang ◽  
Jian Xin He ◽  
Yan Wang
Keyword(s):  
13C Nmr Spectroscopy ◽  
Spectroscopic Characterization ◽  
Random Coil ◽  
Silk Fibers ◽  
Significant Difference ◽  
Sheet Structure ◽  
Α Helix ◽  
Cp Mas Nmr ◽  
Β Sheet

Differences in secondary structure among Bombyx mori (B. mori) silk and two wild silks of Antheraea yamamai (A. yamamai) and Antheraea pernyi (A. pernyi) were investigated by CP/MAS 13C NMR Spectroscopy. The β-sheet structure was primary in three silk, and B. mori silk had the highest β-sheet structure. Although amino acid compositions are very similar for two wild silk, their secondary structures had significant difference. A. yamamai silk contained more α-helix structure, whereas more β-turn and random coil structures formed in A. pernyi silk. B. mori silk was mainly composed of anti-parallel β-sheet structure, however, the parallel β-sheet structure was advantage in the two wild silks, and A. yamamai silk contained more anti-parallel β-sheet conformation than A. pernyi silk.

Conformational Transition of Regenerated Spider Silk in Water

2013 ◽  
Vol 796 ◽  
pp. 107-111
Author(s):  
Ye Mei Zhang ◽  
Zhi Juan Pan
Keyword(s):  
Mechanical Properties ◽  
High Performance ◽  
Spider Silk ◽  
Conformational Transition ◽  
Ph Value ◽  
Random Coil ◽  
Spider Dragline Silk ◽  
Turn Structure ◽  
Α Helix ◽  
Spider Silks

Spider silks have excellent mechanical properties, which can even compare with some high-performance synthetic materials. Although as reported, the impressive mechanical properties are closely related to the primary amino acid sequence, the conformation that molecular chains form is also an important determinant. In this paper, effects of solvent, pH value, temperature, centrifugation and concentrating on the secondary structure of regenerated Ornithoctonus huwenna spider dragline silk protein aqueous solution were investigated by circular dichroism. Spidroin solutions prepared from different LiBr solutions had a distinct combination of secondary structures. The increasing temperature and concentrating can promote the formation of β-sheet structure. While centrifugation was opposite, which elevate the content of β-turn structure. Circular dichroic spectra quantitatively verified an increased α-helix structure content but a decrease of random coil and β-turn structure content with the increasing of pH value.

scholarly journals Fabrication and Characterization of Lignin/Dendrimer Electrospun Blended Fiber Mats

2020 ◽  
Author(s):  
Somaye Akbari ◽  
Addie Bahi ◽  
Ali Farahani ◽  
Abbas Milani ◽  
Frank Ko
Keyword(s):  
Thermal Characteristics ◽  
Dendritic Polymers ◽  
Amino Groups ◽  
Fiber Mats ◽  
Electrospinning Method ◽  
Potential Applications ◽  
Solution Electrospinning ◽  
Nanofiber Mats ◽  
Softwood Kraft Lignin

Blending lignin as the second most abundant polymer in nature with nanostructured compounds such as dendritic polymers will not only add value to lignin, but also increase its application in various fields. In this study, softwood Kraft lignin/polyamidoamine dendritic polymer (PAMAM) blends were fabricated by solution electrospinning method to produce bead-free nanofiber mats. The mats were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR), zeta potential, and thermogravimetry analysis (TGA). The chemical intermolecular interactions between lignin functional groups and abundant amino groups in PAMAM were investigated by FTIR and viscosity measurement. These interactions enhanced the mechanical and thermal characteristics of lignin/PAMAM mats, providing further potential applications at industry level.

Preparation and Characterization of Wool Keratin/PVA Blended Films

2011 ◽  
Vol 175-176 ◽  
pp. 132-136 ◽  
Author(s):  
Ming Xia Gou ◽  
Xu Hong Yang
Keyword(s):  
Aqueous Solution ◽  
Random Coil ◽  
Ftir Analysis ◽  
Microscopy Investigation ◽  
Electron Microscopy Investigation ◽  
Scanning Electron Microscopy Investigation ◽  
Α Helix ◽  
Β Sheet ◽  
Wool Keratin

The method of extracting protein from wool was studied for the purpose of reusing the waste wool. The aqueous solution of wool keratin was prepared with Sodium Shlfide as reductive agent. In this paper, PVA was used to mix with keratin in different proportions. Both solutions were cast to obtain blended films. Scanning electron microscopy investigation showed that the surface of blended films was rough and uneven and the surface of the pure keratin film had small peridiole. FTIR analysis indicated that the secondary structure of the keratin was influenced by the blending ratios. Compared with wool fiber, the keratin film cast from aqueous solution showed a decrease in the amount of α-helix structure, while β-sheet and random coil conformations increased. When the keratin solution and PVA solution were blended in the ratios of 40:60, the film was flexible and rigid, and had good mechanical properties. This study encourages the further investigation of the applications of wool keratin films in the biomedical field, which could provide a new way to reuse various waste feathers.

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