Research on Extraction of Proanthocyanidin from Peanut Skin by Aspergillus niger Fermentation

2011 ◽  
Vol 236-238 ◽  
pp. 2499-2504 ◽  
Author(s):  
Chu Shu Zhang ◽  
Qing Li Yang ◽  
Ke Jia ◽  
Zhi Qiang Liu ◽  
Li Na Yu ◽  
...  

The cell wall of peanut skin was destroyed by cellulase enzymolysis from peanut skin fermentation, which was beneficial to extract proanthocyanidin. The extraction conditions of proanthocyanidin from fermented peanut skin were studied according to orthogonal experiment based on single factor experiments. And the optimum conditions were obtained as follows: extraction time 40min, extraction temperature 55°C, Concentrations of ethanol 45%, solid-liquid ratio 1:15. The extraction rate was 16.17 %.

2019 ◽  
Vol 11 (11) ◽  
pp. 1607-1611
Author(s):  
Lifeng Cheng ◽  
Shengwen Duan ◽  
Xiangyuan Feng ◽  
Ke Zheng ◽  
Qi Yang ◽  
...  

Pectin was extracted from Ramie [Boehmeria nivea (L.) Gaud] through ammonium oxalate extraction method. The influences of single factors (ammonium oxalate concentration, extraction time, solid–liquid ratio, and reaction temperature) on the yield of ramie pectin were studied. Orthogonal experiment was then performed to optimize the combination of the given conditions using pectin yield as the measuring indicator. Finally, the partial physicochemical properties of ramie pectin extracted in the optimal conditions were analyzed. Results showed that the optimal conditions for single-element experiment were 3.5 h (extraction time), 1:25 (solid–liquid ratio), 0.85% (concentration of ammonium oxalate) and 95 °C (extraction temperature), and that the optimal combination conditions for orthogonal experiment was 4 h, 1:30, 0.95% and 90 °C. The ramie pectin yield extracted in optimal conditions reached 15.81%, and its protein content, galacturonic acid content, and esterification degree were 0.659 mg/g, 49.92%, and 53.11%, respectively. Therefore, the proposed method was successfully applied to obtain ramie pectin and may provide scientific basis for improving the multi-utilization value of ramie.


2021 ◽  
Vol 251 ◽  
pp. 02052
Author(s):  
Feng Xuehua ◽  
Song Zurong ◽  
Tao Ali ◽  
Gong Panpan

Using pitaya as raw material, this paper used ethanol subsiding method to study the extraction technology of pitaya polysaccharide by single factor test and orthogonal test. Through orthogonal test, we have found the optimum extraction conditions of pitaya polysaccharide as follows: extraction temperature 60℃, solid-liquid ratio 1:30, extraction time 3h. Under the optimal extraction conditions, the yield of pitaya polysaccharide was 1.13%.


2013 ◽  
Vol 781-784 ◽  
pp. 1194-1198
Author(s):  
Yan Ge ◽  
Rong Kai Ma ◽  
Li Sheng Wang ◽  
Xuan Hai Li ◽  
Xuan Luo

Single factor experiments and orthogonal experiments were successively carried out to optimize the water extraction technology of flavonoids in Thlaspi arvense Linn. Results showed that the influential order of factors was extraction times >solid-liquid ratio >extraction time. The optimum conditions were adding 16 times amount of water, extracting 3 times and every time with 4 hours. Under these conditions, the yield of flavonoids from Thlaspi arvense Linn was 1.17%.


2011 ◽  
Vol 366 ◽  
pp. 421-424 ◽  
Author(s):  
Jiang Nan Zeng ◽  
Bo Quan Jiang ◽  
Zheng Qiang Xiao ◽  
Shu Huan Li

The extraction of collagen from fish scales with papain under ultrasonic pretreatment was conducted in this paper. The effects of the ultrasonic pretreatment time, the ratio of papain to fish scales, the extraction temperature and the extraction time on the extraction rate of collagen were investigated by single factor experiment and orthogonal experiment, respectively. The results showed that the optimum conditions for collagen extraction from fish scales were: ultrasonic pretreatment time 4min, ratio of papain to fish scales 4%, temperature 60°C and extraction time 5h. Under the optimum conditions the extraction rate of collagen reached 90.7%.


2013 ◽  
Vol 634-638 ◽  
pp. 1016-1021
Author(s):  
Li Sheng Wang ◽  
Wei Wang ◽  
Yan Ge ◽  
Jiangtao Tang

Single factor experiments and orthogonal experiments were successively carried out to optimize the water extraction technology of flavonoids in Emilia sonchifolia (Linn.) DC. The results showed that the influential order of factors was extraction times >solid-liquid ratio >extraction time. The optimum conditions were 15:1 of solid-liquid ratio, 2h of the extraction time and 3 of the extraction times. Under these conditions, the extraction yield of flavonoids from Emilia sonchifolia (Linn.) DC was 3.35%.


2021 ◽  
Vol 267 ◽  
pp. 02010
Author(s):  
Rui Wang ◽  
Xueqin Zhang ◽  
Qian Zhou

Moringa leaves were used as raw materials, ethanol and ammonium sulfate were used to construct a aqueous two-phase system to extract the flavonoids from Moringa leaves. The solid-liquid ratio, ethanol content, ultrasonic extraction time, and ultrasonic extraction temperature were selected as single factors, and the extraction process of flavonoids was optimized through single-factor experiments and orthogonal experiments. The optimal process was: 1:110 of solid-liquid ratio, 45 % of ethanol concentration, 15 minutes of ultrasonic extraction time, 70 °C of ultrasonic extraction temperature. Under this condition, the extraction rate of Moringa leaf flavonoids reached 8.37 %.


2014 ◽  
Vol 955-959 ◽  
pp. 2880-2884
Author(s):  
Xiu Hua Tang ◽  
Feng Zhen Zhang

In this paper, the ultrasonic extraction method of Oligomeric Proantho Cyanidin with chestnut shells as raw materials is studied. Using single factor method we study the effects of different extraction time, extraction reagent, extracting agent concentration and ultrasonic power on the extraction rate of Oligomeric Proantho Cyanidin. After analyzing these various factors, we make the orthogonal experiment with the factors including extraction time, extraction agent concentration, extraction temperature, solid-liquid ration, and get the optimal conditions. The experimental results show that the optimum extraction conditions are as follow: time of 30 min, extracting agent of 40% (v %) ethanol aqueous solution, extraction temperature of 40 °C, solid-liquid ratio of 1: 25 g/mL and ultrasonic power of 100 w; under these conditions the extraction rate of Oligomeric Proantho Cyanidin is 3.418%.


2013 ◽  
Vol 781-784 ◽  
pp. 1886-1890
Author(s):  
Fei Long Sun ◽  
Zhang Peng Li ◽  
Ting Li ◽  
Yong Fan ◽  
Yan Chu ◽  
...  

Natural pigments have raised a growing interest due to their extensive range of colors, innocuous and beneficial health effects. In this paper, the extraction process and stability of strawberry red pigment were studied. An orthogonal test [L16(4)4] was applied to get the best extraction conditions. Results showed that the optimum conditions were that the extraction temperature was 65C, the solid-liquid ratio was 1:5 (g/mL), the ratio of 1.5mol/L hydrochloride to 97.5% ethanol was 3:17 (mL/mL), and the extraction time was 4.5 hours. The effects of heat treatment, oxidant, sugar and preservatives on the stability of strawberry red pigment were also discussed. The stability of strawberry red pigment was found to be affected by high temperature, hydrogen peroxide, and potassium sorbate. This study is expected to provide a basis for further research, development, and utilization of strawberry red pigment.


2013 ◽  
Vol 634-638 ◽  
pp. 1552-1556 ◽  
Author(s):  
Yu Qiong Wu ◽  
Wei Dong Man

Commercial crude tea saponin as raw material, ultrasonic assisted extraction technology of tea saponin was investigated. Five influence factors of ethanol concentration, extraction temperature, extraction time, solid-liquid ratio, ultrasonic power on the extraction of tea saponin were studied in detail. Orthogonal experiment through the single factor experiment design, the experimental results show that the optimum operating conditions for the ethanol concentration of 80%, solid-liquid ratio 1: 40, extraction temperature 40°C, ultrasonic power of 300W, ultrasonic extraction time 45 minutes. Under the optimum conditions obtained tea saponin extract with ethanol- acetone precipitation method is further purified, tea saponin content increased from 52.1% to 93.6%. The result indicated that the purity of the tea saponin could be effectively improved through the ultrasonic assisted- precipitation combined method.


2011 ◽  
Vol 291-294 ◽  
pp. 2586-2590
Author(s):  
Hui Wang ◽  
Ling Ma ◽  
Wei Ma

In this study, the soakage extraction method and the ultrasonic extraction method were used to extract active ingredients from Artemisia annua, Artemisia sieversiana, Artemisia mongolica,Artemisia scoparia, Artemisia lavandulaefolia and Artemisia japonica. Using the orthogonal experiment design, we studied the effects of four factors: extraction time, solid-liquid ratio, fineness and extraction times, on extracting active ingredients from Artemisia annua. We also optimized the extraction conditions of the ultrasonic wave method. The results showed that 95% ethanol solution was the best solvent for extracting components in these six species of Artemisia. The best extraction method was ultrasonic extraction method. The optimum extraction conditions of ultrasonic extraction were 1:20 of solid-liquid ratio, one time of extraction, 40 of degree of fineness and 40 minutes of extraction time.


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