Study on Antheraea Pernyi Silk Fibroin Porous Materials

2011 ◽  
Vol 332-334 ◽  
pp. 1718-1721
Author(s):  
Li Mao ◽  
Xi Long Wu ◽  
Yu Liu ◽  
Shen Zhou Lu
Keyword(s):  
Porous Materials ◽  
Silk Fibroin ◽  
Average Pore Size ◽  
Diffraction Method ◽  
Random Coil ◽  
X Ray Diffraction ◽  
Average Pore ◽  
Antheraea Pernyi ◽  
Thiocyanate Solution ◽  
Α Helix

Antheraea pernyi silk fibroin (ASF) solution was prepared by dissolving Antheraea pernyi silk fiber in lithium thiocyanate solution. The ASF/1,4-butanediol (ASF/BDO) blend porous materials were prepared with freeze-drying method. The structure of ASF was investigated by the X-ray diffraction method. The result showed that the structure of regenerated ASF scaffold was α-helix and random coil conformation. After ASF mixed with 5 wt% BDO, the structure of ASF was changed to β-sheet and the ASF scaffolds became water-insoluble. There were some small pores and fibrous structure in the big pores and the surface of pores was rough with a great many raised particles. The scaffolds with the average pore size of 300-1000μm and the porosity of 82-92% can efficiently be produced in this paper. Due to avoid the use of organic solvents or harsh chemicals, these new ASF based porous materials provide much more excellent biocompatibility and is expected to be applied to tissue regeneration scaffolds.

Preparation of Water-Insoluble Antheraea Pernyi Silk Fibroin Films

2012 ◽  
Vol 569 ◽  
pp. 311-315 ◽  
Author(s):  
Shen Zhou Lu ◽  
Li Mao ◽  
Yu Liu ◽  
Shan Sun ◽  
Gui Jun Li
Keyword(s):  
Silk Fibroin ◽  
Diffraction Method ◽  
Random Coil ◽  
X Ray Diffraction ◽  
Antheraea Pernyi ◽  
Blend Film ◽  
Blend Films ◽  
Thiocyanate Solution ◽  
Α Helix ◽  
Β Sheet

Antheraea pernyi silk fibroin (ASF) solution was prepared by dissolving Antheraea pernyi silk fiber in lithium thiocyanate solution. The ASF/glycol blend films were prepared by casting aqueous solution of ASF mixed with glycol. The structure of blend film was investigated by the X-ray diffraction method and infrared spectroscopy. The result showed that the structure of regenerated ASF film was α-helix and random coil conformation. After mixing with glycol, it resulted in significant increase in β-sheet structure with the improvement of water resistance of the films. This effect was strongly dependent on glycol content in the blend film. When the glycol content was more than 45 wt%, the structure of ASF changed to β-sheet and the film became water-insoluble. The breaking strength and elongation of ASF/glycol blend film were 30 Mpa and 50 %, respectively. In summary, the ASF/glycol blend film provided a great potential as a biological material.

Ultrasonication-Induced Rapid Gelation of Wild Silkworm Silk Fibroins

2013 ◽  
Vol 634-638 ◽  
pp. 1165-1169 ◽  
Author(s):  
Gui Yang Liu ◽  
Si Yong Xiong ◽  
Ren Chuan You ◽  
Ling Shuang Wang ◽  
Ming Zhong Li
Keyword(s):  
Secondary Structure ◽  
Silk Fibroin ◽  
Gelation Time ◽  
Random Coil ◽  
X Ray Diffraction ◽  
Freeze Dried ◽  
Silk Fibroins ◽  
Wild Silkworm ◽  
Α Helix ◽  
Silkworm Silk

Silk fibroin （SF） hydrogels of the wild silkworm species Antheraea pernyi and Antheraea yamamai were obtained from aqueous SF solutions at room temperature. Both A. pernyi and A. yamamai solutions were slow to gelate. Hydrogels of the two species of wild silkworm were obtained rapidly following ultrasonicaton at 400–500 W. The secondary structure of the freeze-dried SF hydrogels was measured by X-ray diffraction and Fourier transform infrared spectroscopy. Ultrasonication did not change the main secondary structure of the hydrogels, but it accelerated the structural transformation of silk fibroin molecules from random coil or α helix to β sheet and reduced the gelation time.

Structure of Antheraea pernyi/Bombyx mori Silk Fibroin Scaffolds

2012 ◽  
Vol 535-537 ◽  
pp. 2321-2325 ◽  
Author(s):  
Xiufang Wu ◽  
Yu Hong Jiao ◽  
Gui Yang Liu ◽  
Shu Qin Yan ◽  
Ming Zhong Li
Keyword(s):  
Pore Structure ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Pore Diameter ◽  
Tissue Engineering Scaffolds ◽  
Average Pore ◽  
Antheraea Pernyi ◽  
Condensed Structure ◽  
Α Helix ◽  
Blend Ratios

As the tissue engineering scaffolds, the pore structure and condensed structure of silk fibroin scaffolds should be adjusted and controlled. In this study, Antheraea pernyi/Bombyx mori (A. p/B. m) silk fibroin blend scaffolds were prepared by freeze-drying. The influence of blend ratios on the pore structure and condensed structure of the scaffolds was investigated. The results showed that the average pore diameter of the blend scaffolds changed from 56 to 326 μm. Due to the difference of properties and the macromolecules aggregation status of two silk fibroin solutions, the pore diameter, content of α-helix and crystallinity of the scaffolds decreased with the increasing of the proportion of B. m silk fibroin. By adjusting the blend ratios, the pore structure and condensed structure of A. p/B. m silk fibroin blend scaffolds could be controlled.

Study on Silk Fibroin Gelation: Effect of Polyalcohol

2011 ◽  
Vol 175-176 ◽  
pp. 137-142 ◽  
Author(s):  
Qiang Zhang ◽  
Ying Dong Cheng ◽  
Yu Liu ◽  
Shu Qin Yan ◽  
Ming Zhong Li
Keyword(s):  
Silk Fibroin ◽  
Three Dimensional ◽  
Random Coil ◽  
X Ray Diffraction ◽  
Polyethylene Glycol 400 ◽  
Bombyx Mori Silk ◽  
Α Helix ◽  
Β Sheet ◽  
Important Form ◽  
Fibroin Solution

The Bombyx mori silk fibroin gel with three dimensional structures is an important form to be developed for tissue engineering materials. In this paper, silk fibroin gels were prepared with adding polyalcohol into silk fibroin solution. The gel structure was analyzed by X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. The results demonstrated that when adding more than 100% of polyalcohol, both of glycerol and polyethylene glycol 400 (PEG400) can accelerate the gelation process markedly. With the increase of the percentage of PEG400 and glycerol, it promoted silk fibroin molecules to cluster rapidly and inhibit silk fibroin molecules transforming from the random coil or α-helix to β-sheet in a ratio of 900% especially. Silk fibroin gels containing 100% of polyalcohol had more uniform morphology and the pores distributed uniformly.

Polymer Embedded Nanocrystalline Titania Sorbent for 99Mo-99mTc Generator

2008 ◽  
Vol 8 (9) ◽  
pp. 4447-4452 ◽  
Author(s):  
Rubel Chakravarty ◽  
Rakesh Shukla ◽  
Shyamla Gandhi ◽  
Ramu Ram ◽  
Ashutosh Dash ◽  
...  
Keyword(s):  
Saline Solution ◽  
Average Pore Size ◽  
Average Crystallite Size ◽  
Activity Level ◽  
X Ray Diffraction ◽  
Average Pore ◽  
X Ray ◽  
Nano Crystalline ◽  
99Mtc Generator

A new sorbent material, polymer embedded nano crystalline titania (Titanium Polymer-TiP) has been developed, from titanium (IV) chloride and isopropyl alcohol, for the adsorption of 99Mo, which is a precursor to 99mTc, a workhorse in radio-pharmaceuticals. The infrared absorption spectra of the TiP showed peaks corresponding to Ti-O groups. X-ray diffraction pattern of the adsorbent corresponded to rutile TiO2. The surface area of this polymer was 30 m2/g with an average pore size of 40 nm. The average crystallite size of TiO2, embedded in polymer, was found to be 5 nm. TEM micrograph of the adsorbent revealed the networkof polymer with dispersed titania phase. Potential of this adsorbent for the preparation of 99Mo-99mTc generator has been explored. 99Mo could be adsorbed on to the adsorbent column containing TiP at pH 1 from which 99mTc could be eluted with normal (0.9%) saline solution with an elution yield of ∼80%. The quality of the 99mTcO−4 obtained was in accordance with the international specifications applicable for radiopharmaceutical use. A process demonstration run was carried out with 1.1 GBq (30 mCi) 99Mo activity level making use of the above adsorbent and consistent results were obtained over a period of one week, which is generally the shelf life of 99Mo-99mTc generator.

Regenerated Antheraea pernyi Silk Fibroin Nanofiber Film

2012 ◽  
Vol 465 ◽  
pp. 160-164 ◽  
Author(s):  
Li Mao ◽  
Yu Liu ◽  
Xi Long Wu ◽  
Shen Zhou Lu
Keyword(s):  
Surface Tension ◽  
Silk Fibroin ◽  
Self Assembly ◽  
Water Environment ◽  
Antheraea Pernyi ◽  
Thiocyanate Solution ◽  
Nanofiber Film ◽  
20 Nm ◽  
Cell Migration And Proliferation

Regenerated Antheraea Pernyi silk fibroin (ASF) solution was prepared by dissolving Antheraea Pernyi (A. pernyi) silk fiber in lithium thiocyanate solution. Atomic force microscopy (AFM) examination showed that there were many short nanofibers in regenerated ASF solution, which were made up of 10-20 nm nanospheres. In this paper, we discussed the formation mechanism of these nanofibers. The results of the surface tension showed that ASF had surface active and can significantly decline the surface tension of water from 73 mN/m to 51 mN/m. The hydrophobic side chains of ASF molecular would try to break away from water environment and thereby undergo self-assembly into nanospheres. And then, these nanospheres arrange into a short nanofiber. Then, the ASF nanofiber film was prepared by casting regenerated ASF solution blended with glycol. The structure of ASF was β-sheet and the film was water-insoluble. This blend film not only had excellent mechanical properties, but also can be biodegradated by enzyme in vitro. All of this indicated that this new regenerated ASF nanofiber film not only provided excellent biocompatibility, but also had large surface as extracellular matrix (ECM) to cell adhesion. The film made of nanofiber was similar to ECM on the nanoscale, which promoted cell migration and proliferation. In summary, it provided a great potential as a biological material.

EDC-Crosslinked Electrospun Silk-Fibroin Fiber Mats

2011 ◽  
Vol 175-176 ◽  
pp. 170-175 ◽  
Author(s):  
Rong Liu ◽  
Feng Zhang ◽  
Bao Qi Zuo ◽  
Huan Xiang Zhang
Keyword(s):  
Silk Fibroin ◽  
Rupture Strength ◽  
Mechanical Test ◽  
Crosslinking Agent ◽  
Water Soluble ◽  
Random Coil ◽  
Crosslinking Reaction ◽  
Elongation Ratio ◽  
Silk Fibroin Fiber ◽  
Α Helix

Electrospun Silk-Fibroin (SF) mats were fabricated by electrospinning with regenerated Bombyx mori silk-fibroin/formic acid solutions. After spinning, the water soluble and mechanical properties of pure fibroin nanofibers were poor. So electrospun SF mats were crosslinked with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), a low cell cytotoxicity crosslinking agent, and N-hydroxysuccinimide (NHS), which can increase the reaction rate. The scanning electron microscope images indicated that the diameter of fibers increased with crosslinking reaction. When EDC/NHS reached to 7.5wt.%, the diameter of fibers achieved the maximum. The mechanical test showed that tensile strength enhanced after crosslinking with EDC/ NHS. While EDC/NHS reached to 7.5wt %, the rupture strength reached to (38.31±5.30) Mpa, and the breaking elongation ratio reached to (182.00±31.27) %. FTIR results showed the the proportion of β-sheet increased while random coil and α-helix decreased after treatment.

scholarly journals Fabrication of Porous Bone Scaffolds Using Alginate and Bioactive Glass

2019 ◽  
Vol 10 (1) ◽  
pp. 15 ◽  
Author(s):  
Jonathan Hatton ◽  
Graham Roy Davis ◽  
Abdel-Hamid I. Mourad ◽  
Nizamudeen Cherupurakal ◽  
Robert G. Hill ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Pore Size ◽  
Composite Scaffold ◽  
Average Pore Size ◽  
Amorphous Structure ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
Average Pore ◽  
X Ray ◽  
Novel Material

Porous composite scaffold using an alginate and bioactive glass ICIE16M was synthesized by a simple freeze-drying technique. The scaffold was characterized using compression testing, Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), X-ray diffraction (XRD), X-ray microtomography (XMT) and scanning electron microscopy (SEM). The bioactivity of the scaffold was evaluated by its ability to form apatite on its surface in simulated body fluid (SBF). The data collected showed evidence that the novel material produced had an appropriate pore size for osteoconduction, with an average pore size of 110 µm and maximum pore size of 309 µm. Statistical analysis confirmed that the glass filler significantly (P < 0.05) increased the collapse yield of the scaffolds compared with pure alginate scaffolds. The ICIE16M glass had an amorphous structure, favorable for bioactivity.

Preparation of Antheraea pernyi Silk Fibroin/Chitosan Blend Scaffolds

2011 ◽  
Vol 140 ◽  
pp. 12-16
Author(s):  
Ting Zhao ◽  
Ceng Zhang ◽  
Qiang Tang ◽  
Shu Qin Yan ◽  
Ming Zhong Li
Keyword(s):  
Cell Adhesion ◽  
Silk Fibroin ◽  
Freeze Drying ◽  
Pore Diameter ◽  
Freezing Temperature ◽  
Process Conditions ◽  
Average Pore ◽  
Antheraea Pernyi ◽  
Preparation Conditions ◽  
The Relationship

In this work, Antheraea pernyi silk fibroin (ASF) /chitosan (CS) blend scaffolds were prepared by freeze-drying. The relationship between preparation conditions and morphological structures of blend scaffolds was also studied. The results indicated that the porosity and pore diameter of the ASF /CS scaffolds decreased with increasing of the proportion of chitosan and decreasing of the freezing temperature. By changing the process conditions, the average pore diameter and porosity of ASF/CS scaffolds could be adjusted in the range of 134 - 527 μm and 71 - 91% respectively. Compared with the pure ASF or CS materials, the ASF / CS (60/40) blend materials were more favor of cell adhesion.

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