Regenerated Antheraea pernyi Silk Fibroin Nanofiber Film

2012 ◽  
Vol 465 ◽  
pp. 160-164 ◽  
Author(s):  
Li Mao ◽  
Yu Liu ◽  
Xi Long Wu ◽  
Shen Zhou Lu
Keyword(s):  
Surface Tension ◽  
Silk Fibroin ◽  
Self Assembly ◽  
Water Environment ◽  
Antheraea Pernyi ◽  
Thiocyanate Solution ◽  
Nanofiber Film ◽  
20 Nm ◽  
Cell Migration And Proliferation

Regenerated Antheraea Pernyi silk fibroin (ASF) solution was prepared by dissolving Antheraea Pernyi (A. pernyi) silk fiber in lithium thiocyanate solution. Atomic force microscopy (AFM) examination showed that there were many short nanofibers in regenerated ASF solution, which were made up of 10-20 nm nanospheres. In this paper, we discussed the formation mechanism of these nanofibers. The results of the surface tension showed that ASF had surface active and can significantly decline the surface tension of water from 73 mN/m to 51 mN/m. The hydrophobic side chains of ASF molecular would try to break away from water environment and thereby undergo self-assembly into nanospheres. And then, these nanospheres arrange into a short nanofiber. Then, the ASF nanofiber film was prepared by casting regenerated ASF solution blended with glycol. The structure of ASF was β-sheet and the film was water-insoluble. This blend film not only had excellent mechanical properties, but also can be biodegradated by enzyme in vitro. All of this indicated that this new regenerated ASF nanofiber film not only provided excellent biocompatibility, but also had large surface as extracellular matrix (ECM) to cell adhesion. The film made of nanofiber was similar to ECM on the nanoscale, which promoted cell migration and proliferation. In summary, it provided a great potential as a biological material.

scholarly journals In Vitro Assembly of the T=13 Procapsid of Bacteriophage T5 with Its Scaffolding Domain

2010 ◽  
Vol 84 (18) ◽  
pp. 9350-9358 ◽  
Author(s):  
Alexis Huet ◽  
James F. Conway ◽  
Lucienne Letellier ◽  
Pascale Boulanger
Keyword(s):  
Self Assembly ◽  
Scaffolding Protein ◽  
Accessory Proteins ◽  
Conformational Diversity ◽  
In Vitro Assembly ◽  
20 Nm ◽  
Head Protein ◽  
Bacteriophage T5 ◽  
Icosahedral Capsid

ABSTRACT The Siphoviridae coliphage T5 differs from other members of this family by the size of its genome (121 kbp) and by its large icosahedral capsid (90 nm), which is organized with T=13 geometry. T5 does not encode a separate scaffolding protein, but its head protein, pb8, contains a 159-residue aminoterminal scaffolding domain (Δ domain) that is the mature capsid. We have deciphered the early events of T5 shell assembly starting from purified pb8 with its Δ domain (pb8p). The self assembly of pb8p is regulated by salt conditions and leads to structures with distinct morphologies. Expanded tubes are formed in the presence of NaCl, whereas Ca2+ promotes the association of pb8p into contracted tubes and procapsids. Procapsids display an angular organization and 20-nm-long internal radial structures identified as the Δ domain. The T5 head maturation protease pb11 specifically cleaves the Δ domain of contracted and expanded tubes. Ca2+ is not required for proteolytic activity but for the organization of the Δ domain. Taken together, these data indicate that pb8p carries all of the information in its primary sequence to assemble in vitro without the requirement of the portal and accessory proteins. Furthermore, Ca2+ plays a key role in introducing the conformational diversity that permits the formation of a stable procapsid. Phage T5 is the first example of a viral capsid consisting of quasi-equivalent hexamers and pentamers whose assembly can be carried out in vitro, starting from the major head protein with its scaffolding domain, and whose endpoint is an icosahedral T=13 particle.

Controllable transition of silk fibroin nanostructures: An insight into in vitro silk self-assembly process

2013 ◽  
Vol 9 (8) ◽  
pp. 7806-7813 ◽  
Author(s):  
S. Bai ◽  
S. Liu ◽  
C. Zhang ◽  
W. Xu ◽  
Q. Lu ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Self Assembly ◽  
Assembly Process ◽  
Insight Into

Study on Antheraea Pernyi Silk Fibroin Porous Materials

2011 ◽  
Vol 332-334 ◽  
pp. 1718-1721
Author(s):  
Li Mao ◽  
Xi Long Wu ◽  
Yu Liu ◽  
Shen Zhou Lu
Keyword(s):  
Porous Materials ◽  
Silk Fibroin ◽  
Average Pore Size ◽  
Diffraction Method ◽  
Random Coil ◽  
X Ray Diffraction ◽  
Average Pore ◽  
Antheraea Pernyi ◽  
Thiocyanate Solution ◽  
Α Helix

Antheraea pernyi silk fibroin (ASF) solution was prepared by dissolving Antheraea pernyi silk fiber in lithium thiocyanate solution. The ASF/1,4-butanediol (ASF/BDO) blend porous materials were prepared with freeze-drying method. The structure of ASF was investigated by the X-ray diffraction method. The result showed that the structure of regenerated ASF scaffold was α-helix and random coil conformation. After ASF mixed with 5 wt% BDO, the structure of ASF was changed to β-sheet and the ASF scaffolds became water-insoluble. There were some small pores and fibrous structure in the big pores and the surface of pores was rough with a great many raised particles. The scaffolds with the average pore size of 300-1000μm and the porosity of 82-92% can efficiently be produced in this paper. Due to avoid the use of organic solvents or harsh chemicals, these new ASF based porous materials provide much more excellent biocompatibility and is expected to be applied to tissue regeneration scaffolds.

Preparation of Water-Insoluble Antheraea Pernyi Silk Fibroin Films

2012 ◽  
Vol 569 ◽  
pp. 311-315 ◽  
Author(s):  
Shen Zhou Lu ◽  
Li Mao ◽  
Yu Liu ◽  
Shan Sun ◽  
Gui Jun Li
Keyword(s):  
Silk Fibroin ◽  
Diffraction Method ◽  
Random Coil ◽  
X Ray Diffraction ◽  
Antheraea Pernyi ◽  
Blend Film ◽  
Blend Films ◽  
Thiocyanate Solution ◽  
Α Helix ◽  
Β Sheet

Antheraea pernyi silk fibroin (ASF) solution was prepared by dissolving Antheraea pernyi silk fiber in lithium thiocyanate solution. The ASF/glycol blend films were prepared by casting aqueous solution of ASF mixed with glycol. The structure of blend film was investigated by the X-ray diffraction method and infrared spectroscopy. The result showed that the structure of regenerated ASF film was α-helix and random coil conformation. After mixing with glycol, it resulted in significant increase in β-sheet structure with the improvement of water resistance of the films. This effect was strongly dependent on glycol content in the blend film. When the glycol content was more than 45 wt%, the structure of ASF changed to β-sheet and the film became water-insoluble. The breaking strength and elongation of ASF/glycol blend film were 30 Mpa and 50 %, respectively. In summary, the ASF/glycol blend film provided a great potential as a biological material.

scholarly journals Fabrication of Antheraea pernyi Silk Fibroin-Based Thermoresponsive Hydrogel Nanofibers for Colon Cancer Cell Culture

Polymers ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 108
Author(s):  
Bo-Xiang Wang ◽  
Jia Li ◽  
De-Hong Cheng ◽  
Yan-Hua Lu ◽  
Li Liu
Keyword(s):  
Cell Culture ◽  
Colon Cancer ◽  
Cancer Cell ◽  
Silk Fibroin ◽  
Glycidyl Ether ◽  
Polymerization Time ◽  
Antheraea Pernyi ◽  
Thermoresponsive Hydrogel ◽  
Lovo Cells

Antheraea pernyi silk fibroin (ASF)-based nanofibers have wide potential for biomaterial applications due to superior biocompatibility. It is not clear whether the ASF-based nanofibers scaffold can be used as an in vitro cancer cell culture platform. In the current study, we fabricated novel ASF-based thermoresponsive hydrogel nanofibers by aqueous electrospinning for colon cancer (LoVo) cells culture. ASF was reacted with allyl glycidyl ether (AGE) for the preparation of allyl silk fibroin (ASF-AGE), which provided the possibility of copolymerization with allyl monomer. The investigation of ASF-AGE structure by 1H NMR revealed that reactive allyl groups were successfully linked with ASF. ASF-based thermoresponsive hydrogel nanofibers (p (ASF-AGE-NIPAAm)) were successfully manufactured by aqueous electrospinning with the polymerization of ASF and N-isopropylacrylamide (NIPAAm). The p (ASF-AGE-NIPAAm) spinning solution showed good spinnability with the increase of polymerization time, and uniform nanofibers were formed at the polymerization time of 360 min. The obtained hydrogel nanofibers exhibited good thermoresponsive that the LCST was similar with PNIPAAm at about 32 °C, and good degradability in protease XIV PBS solution. In addition, the cytocompatibility of colon cancer (LoVo) cells cultured in hydrogel nanofibers was assessed. It was demonstrated that LoVo cells grown on hydrogel nanofibers showed improved cell adhesion, proliferation, and viability than those on hydrogel. The results suggest that the p (ASF-AGE-NIPAAm) hydrogel nanofibers have potential application in LoVo cells culture in vitro. This study demonstrates the feasibility of fabricating ASF-based nanofibers to culture LoVo cancer cells that can potentially be used as an in vitro cancer cell culture platform.

Antheraea pernyi Silk Fibroin Microspheres Carried Lysozyme

2014 ◽  
Vol 915-916 ◽  
pp. 875-878 ◽  
Author(s):  
Juan Wang ◽  
Gui Jun Li ◽  
Rui Jiang ◽  
Zhu Ping Yin ◽  
Shen Zhou Lu
Keyword(s):  
Silk Fibroin ◽  
Release Kinetics ◽  
In Vitro Release ◽  
X Ray Diffraction ◽  
Antheraea Pernyi ◽  
Encapsulation Rate ◽  
Release Field ◽  
Model Drug ◽  
Vitro Release

A method was developed to prepare the Antheraea Pernyi silk fibroin (ASF) microspheres using lysozyme as a model drug to estimate the application of ASF in drug controlled release field. The structure of ASF microsphere carried drug was characterized by X-ray diffraction and FTIR. The morphology and the influence of the microspheres on the degradation of lysozyme were investigated using scanning electron microscope. The results show that encapsulation rate was increased and drug content was decreased with the addition of lysozyme increasing. In vitro release of lysozyme from the ASF particles we demonstrated that the release kinetics depends on the pH. The pH played important roles in controlling lysozyme release profiles. It also can be seen that the degradation speed of lysozyme ASF microspheres slightly larger than the pure ASF microspheres.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

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