Validation of the Pseudo Imprinted Microspheres for Solid-Phase Extraction of Acyclovir from Swine Tissue

The novel pseudo imprinted microspheres were synthesized by aqueous suspension polymerization using theophylline as the template and employed as a special sorbent of solid-phase extraction for selective extraction of acyclovir from swine tissue. The pseudo imprinted microspheres revealed high affinity and selectivity to acyclovir, and the matrix interferences could be efficiently eliminated, which make the extract of solid-phase extraction clean enough for HPLC analysis. The parameters that affect the extraction efficiency of solid-phase extraction were investigated, including solvent of extraction, volume of loading sample, washing and elution solvent, etc. Good linearity was obtained in a range of 0.05~15 μg/mL with correlation coefficient (r2) of 0.9991. The recoveries at three spiked levels were ranged from 91.4% to101.0% with RSD less than of 5.0%.

Download Full-text

DEVELOPMENT OF MOLECULAR IMPRINTED POLYMER SOLID PHASE EXTRACTION (MISPE) FOR SEPARATION NITROFURANTOIN RESIDUE IN CHICKEN EGGS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i6.17109 ◽

2017 ◽

Vol 10 (6) ◽

pp. 108

Author(s):

Sophi Damayanti ◽

Untung Gunawan ◽

Slamet Ibrahim

Keyword(s):

Solid Phase Extraction ◽

Analytical Methods ◽

Density Functional ◽

Solid Phase ◽

Basis Set ◽

Phase Extraction ◽

Molecular Imprinted Polymer ◽

Imprinted Polymer ◽

Dft Methods ◽

The Matrix

Background: The use of nitrofurantoin and other nitrofuran antibiotics in food which produced from animals is prohibited by European Union because of potentially carcinogenic and mutagenic. Various methods for analysis of residues of nitrofurantoin has been developed, but because of the interference of the matrix, it is necessary to separate the matrix therefore, the matrix effect will not interfere the analysis. Nowadays, molecular imprinted polymer (MIP) is a well-developed tool in the analytical field, mainly for separating substances in relatively complex matrices.Objective: The purpose of this study is to obtain MISPE that is selective for the separation of nitrofurantoin residues in chicken eggs.Methods: Analytical methods development of nitrofurantoin were optimization of HPLC system and validation of analytical methods performed to obtain the suitable system for nitrofurantoin detection. In silico study used for MIP design by observing the difference Gibbs free energy using Gaussview 5.08 software with Density Functional Theory (DFT) methods using 6-311G as basis set. MIP synthesis was done using bulk method use nitrofurantoin as template, acrylamide as functional monomer, ethyleneglycoldimethacrylate (EGDMA) as crosslinker, and azobisisobutyronitrile (AIBN) as an initiator reaction inside dimethylformamide (DMF) as solvent. Non imprinted polymer (NIP) was synthesized as comparison. MIP and NIP which has been synthesized was inserted into SPE cartridge and characterized using Infrared spectroscopy and HPLC.Result: MISPE that has been synthesized was characterized and compared to non-imprinted polymer solid phase extraction (NISPE) and marketed Solid Phase Extraction (SPE) C18. Sensitivity of MIP, NIP, and SPE C-18 to nitrofurantoin was 84.54 %, 37.73 %, and 33.95 % respectively, based on recovery of nitrofurantoin.Conclusion: Based on the result it was obtained MISPE has high selectivity toward nitrofurantoin compared to NISPE and either marketed SPE.

Download Full-text

Molecularly Imprinted Solid-Phase Extraction for Chlorpyrifos Determination in Water Samples

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.113-116.947 ◽

2010 ◽

Vol 113-116 ◽

pp. 947-951 ◽

Cited By ~ 4

Author(s):

Jun Liu ◽

Ming Yang ◽

Lu Feng Huai

Keyword(s):

Solid Phase Extraction ◽

Water Samples ◽

Matrix Effects ◽

Solid Phase ◽

Final Analysis ◽

Sewage Water ◽

Phase Extraction ◽

Simple Method ◽

Molecularly Imprinted ◽

The Matrix

The determination of chlorpyrifos at low ngL-1 levels in wastewater requires highly selective and sensitive analytical procedures. The removal of matrix components during sample preparation results in significant benefits towards reducing the matrix effects during GC analysis. Therefore this work describes a simple method to enrich and clean up chlorpyrifos from sewage water using molecularly imprinted solid phase extraction (MISPE). Final analysis was performed by gas chromatography. The performance of this method has been evaluated in water samples in terms of recovery, precision, and method quantification limit. Recovery for chlorpyrifos ranged between 99 and 105% with RSD values below 7.9%. The performance of the method was further emphasized by the study of different water samples. In these samples, chlorpyrifos were detected in concentration above method quantification limits ranging from 50 to 3000 ngL-1.

Download Full-text

Facile and environmentally friendly magnetic mesoporous carbon for the selective extraction of antioxidants from water

Analytical Methods ◽

10.1039/c9ay00819e ◽

2019 ◽

Vol 11 (32) ◽

pp. 4204-4210 ◽

Cited By ~ 1

Author(s):

Thitima Rujiralai ◽

Sujitra Kaewsara ◽

Laemthong Chuenchom ◽

Panuwat Jitpirom ◽

Sulawan Kaowphong

Keyword(s):

Solid Phase Extraction ◽

Mesoporous Carbon ◽

Solid Phase ◽

Environmentally Friendly ◽

Selective Extraction ◽

Phase Extraction ◽

Magnetic Mesoporous Carbon

Selective solid-phase extraction employing magnetic mesoporous carbon followed by HPLC was successfully developed for the determination of antioxidants in water.

Download Full-text

Selective extraction and elution of weak bases by in-line solid-phase extraction capillary electrophoresis using a pH step gradient and a weak cation-exchange monolith

The Analyst ◽

10.1039/b804805c ◽

2008 ◽

Vol 133 (10) ◽

pp. 1380 ◽

Cited By ~ 17

Author(s):

Jonathan R. E. Thabano ◽

Michael C. Breadmore ◽

Joseph P. Hutchinson ◽

Cameron Johns ◽

Paul R. Haddad

Keyword(s):

Capillary Electrophoresis ◽

Solid Phase Extraction ◽

Cation Exchange ◽

Solid Phase ◽

Selective Extraction ◽

Phase Extraction ◽

Weak Bases ◽

Weak Cation Exchange

Download Full-text

Selective Solid-Phase Extraction Using Molecularly Imprinted Polymers for the Analysis of Target Pesticides in Cannabis Bud

South African Journal of Chemistry ◽

10.17159/0379-4350/2021/v75a17 ◽

2021 ◽

Vol 75 ◽

Author(s):

Paul Myburgh ◽

Gerdus Kemp ◽

Patricia Forbes

Keyword(s):

Solid Phase Extraction ◽

Solid Phase ◽

Treatment Method ◽

Template Molecule ◽

Phase Extraction ◽

Imprinted Polymer ◽

Molecularly Imprinted ◽

Matrix Interferences ◽

Sample Matrix ◽

Ms Analysis

ABSTRACT A molecularly imprinted solid-phase extraction (MISPE) procedure was developed for the GC-MS analysis of four high priority pesticides (atrazine, terbuthylazine, acetochlor and alachlor) in a cannabis bud sample matrix. The study demonstrated that the synthesised polymer had a high affinity and good selectivity for either chloroacetamide or triazine classes of pesticide used as a template molecule during the molecularly imprinted polymerisation reaction. The MISPE procedure was optimised in terms of loading, washing and elution fractions utilising a range of aqueous methanol solutions for optimal recovery and minimal matrix interferences. The optimal wash fraction was 20% (v/v) methanol in an aqueous solution, whilst 70% (v/v) was used for the elution fraction. The selectivity, accuracy and recovery of the MISPEs were verified using a synthesised non-imprinted polymer and a commercial C18 cartridge as reference sorbents in comparative experiments. Approximately 3 g of the cannabis bud sample was spiked at a 0.05 mg/kg maximum residue limit (MRL) concentration. The recovery of the four selected pesticides extracted from the spiked samples ranged between 76.4-85.0% when utilising the optimised MISPE methods, compared to 91.6-96.9% for the C18 SPE. However, the use of the MISPE resulted in enhanced selectivity, as evidenced by GC-MS analysis, due to the extraction of less matrix interferences. Therefore, it can be concluded that the MISPE is a viable pre-treatment method for selective pesticide analysis in cannabis flowers using GC-MS when selectivity is valued for the extraction of target pesticides from a complex sample matrix. Keywords: molecularly imprinted polymer; solid-phase extraction; Cannabis; pesticides; atrazine; terbuthylazine; acetochlor; alachlor

Download Full-text

Urinary l -kynurenine quantification and selective extraction through a molecularly imprinted solid-phase extraction device

Journal of Separation Science ◽

10.1002/jssc.201800458 ◽

2018 ◽

Vol 41 (16) ◽

pp. 3204-3212 ◽

Cited By ~ 4

Author(s):

Lucia Mergola ◽

Ciriana Orabona ◽

Elisa Albini ◽

Giuseppe Vasapollo ◽

Sonia Scorrano ◽

...

Keyword(s):

Solid Phase Extraction ◽

Solid Phase ◽

Selective Extraction ◽

Phase Extraction ◽

Molecularly Imprinted ◽

Extraction Device

Download Full-text

Solid-phase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals

Journal of Chromatography A ◽

10.1016/j.chroma.2010.04.071 ◽

2010 ◽

Vol 1217 (43) ◽

pp. 6668-6673 ◽

Cited By ~ 71

Author(s):

Wassim Hadj Ali ◽

Delphine Derrien ◽

Florent Alix ◽

Céline Pérollier ◽

Olivier Lépine ◽

...

Keyword(s):

Solid Phase Extraction ◽

Molecularly Imprinted Polymers ◽

Solid Phase ◽

Selective Extraction ◽

Phase Extraction ◽

Molecularly Imprinted ◽

Imprinted Polymers

Download Full-text

Dispersive Solid Phase Extraction for the Analysis of Veterinary Drugs Applied to Food Samples: A Review

International Journal of Analytical Chemistry ◽

10.1155/2017/8215271 ◽

2017 ◽

Vol 2017 ◽

pp. 1-16 ◽

Cited By ~ 27

Author(s):

Gabriela Islas ◽

Israel S. Ibarra ◽

Prisciliano Hernandez ◽

Jose M. Miranda ◽

Alberto Cepeda

Keyword(s):

Solid Phase Extraction ◽

Solid Phase ◽

Food Samples ◽

Veterinary Drugs ◽

Phase Extraction ◽

Dispersive Solid Phase Extraction ◽

Solid Sorbent ◽

Wide Range ◽

The Matrix ◽

Carbon Based Nanomaterials

To achieve analytical success, it is necessary to develop thorough clean-up procedures to extract analytes from the matrix. Dispersive solid phase extraction (DSPE) has been used as a pretreatment technique for the analysis of several compounds. This technique is based on the dispersion of a solid sorbent in liquid samples in the extraction isolation and clean-up of different analytes from complex matrices. DSPE has found a wide range of applications in several fields, and it is considered to be a selective, robust, and versatile technique. The applications of dispersive techniques in the analysis of veterinary drugs in different matrices involve magnetic sorbents, molecularly imprinted polymers, carbon-based nanomaterials, and the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method. Techniques based on DSPE permit minimization of additional steps such as precipitation, centrifugation, and filtration, which decreases the manipulation of the sample. In this review, we describe the main procedures used for synthesis, characterization, and application of this pretreatment technique and how it has been applied to food analysis.

Download Full-text