Adsoption CD34 Antibody onto the Col/Hep Coating Film on Titanium to Improve Cytocompatibility of Titanium

2008 ◽  
Vol 47-50 ◽  
pp. 1411-1414 ◽  
Author(s):  
Jia Long Chen ◽  
Quan Li Li ◽  
Jun Ying Chen ◽  
Nan Huang
Keyword(s):  
Self Assembly ◽  
Electrostatic Interactions ◽  
Layer By Layer ◽  
Coating Film ◽  
Test Results ◽  
Cardiovascular Devices ◽  
Assembly Technique ◽  
Confluent Cell ◽  
Good Potential ◽  
Positively Charged

This study deals with improving the cytocompatibility of titanium by a coating with heparin(hep), collagen(col) and CD34 antibody. Collagen and heparin molecules were adsorbed onto a titanium surface using a layer-by-layer (LBL) self-assembly technique. The col/hep coating were formed by alternating deposition of negatively charged heparin and positively charged collagen based on electrostatic interactions. Finally the CD34 antibody was absorbed onto the surface of col/hep coating by electrostatic interactions. The chemical composition and surface topography were investigated by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The fluorescence microscopy images proved that the CD34 antibody was existed on the surface of the col/hep coating. The SEM results and the MTT test results showed that, compared to the surface of col/hep coating and the surface of titanium, the CD34 surface had better endothelial progenitor cells (EPCs) attachment and proliferation. The EPCs on the CD34 surface displayed the morphology of flat endothelium, and a confluent cell layer after cultured for 2d. This study suggested that this method maybe have good potential for surface modification of cardiovascular devices.

Multilayer composite beads constructed via layer-by-layer self-assembly for lysozyme controlled release

RSC Advances ◽  
2014 ◽  
Vol 4 (46) ◽  
pp. 24369-24376 ◽  
Author(s):  
Jiemin Zhao ◽  
Xiaoping Wang ◽  
Yanshen Kuang ◽  
Yufeng Zhang ◽  
Xiaowen Shi ◽  
...  
Keyword(s):  
Controlled Release ◽  
Self Assembly ◽  
Cross Linking ◽  
Layer By Layer ◽  
Multilayer Composite ◽  
Composite Beads ◽  
Assembly Technique ◽  
Positively Charged

Alginate (ALG)–lysozyme (LZ) beads were fabricated by a cross-linking process. Negatively charged ALG and positively charged LZ were alternately deposited on the positively charged ALG–LZ beads via a layer-by-layer (LBL) self-assembly technique.

scholarly journals Surface Modification of Titanium with Heparin-Chitosan Multilayers via Layer-by-Layer Self-Assembly Technique

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Yao Shu ◽  
Guomin Ou ◽  
Li Wang ◽  
Jingcai Zou ◽  
Quanli Li
Keyword(s):  
Surface Modification ◽  
Self Assembly ◽  
Pure Titanium ◽  
Titanium Substrate ◽  
Titanium Implants ◽  
Layer By Layer ◽  
Assembly Technique ◽  
Titanium Surfaces ◽  
Modification Of Titanium ◽  
Positively Charged

Extracellular matrix (ECM), like biomimetic surface modification of titanium implants, is a promising method for improving its biocompatibility. In this paper chitosan (Chi) and heparin (Hep) multilayer was coated on pure titanium using a layer-by-layer (LbL) self-assembly technique. The Hep-Chi multilayer growth was carried out by first depositing a single layer of positively charged poly-L-lysine (PLL) on the NaOH-treated titanium substrate (negatively charged surface), followed by alternate deposition of negatively charged Hep and positively charged Chi, and terminated by an outermost layer of Chi. The multilayer was characterized by DR-FTIR, SEM, and AFM, and osteoblasts were cocultured with the modified titanium and untreated titanium surfaces, respectively, to evaluate their cytocompatibilityin vitro. The results confirmed that Hep-Chi multilayer was fabricated gradually on the titanium surface. The Hep-Chi multilayer-coated titanium improved the adhesion, proliferation and differentiation of osteoblasts. Thus, the approach described here may provide a basis for the preparation of modified titanium surfaces for use in dental or orthopedic implants.

scholarly journals Fabrication and in vitro evaluation of stable collagen/hyaluronic acid biomimetic multilayer on titanium coatings

2013 ◽  
Vol 10 (84) ◽  
pp. 20130070 ◽  
Author(s):  
Haiyong Ao ◽  
Youtao Xie ◽  
Honglue Tan ◽  
Shengbing Yang ◽  
Kai Li ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Photoelectron Spectroscopy ◽  
Self Assembly ◽  
Human Mesenchymal Stem Cells ◽  
Biological Properties ◽  
Covalent Immobilization ◽  
Layer By Layer ◽  
Titanium Coating ◽  
Assembly Technique

Layer-by-layer (LBL) self-assembly technique has been proved to be a highly effective method to immobilize the main components of the extracellular matrix such as collagen and hyaluronic acid on titanium-based implants and form a polyelectrolyte multilayer (PEM) film by electrostatic interaction. However, the formed PEM film is unstable in the physiological environment and affects the long-time effectiveness of PEM film. In this study, a modified LBL technology has been developed to fabricate a stable collagen/hyaluronic acid (Col/HA) PEM film on titanium coating (TC) by introducing covalent immobilization. Scanning electron microscopy, diffuse reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to characterize the PEM film. Results of Sirius red staining demonstrated that the chemical stability of PEM film was greatly improved by covalent cross-linking. Cell culture assays further illustrated that the functions of human mesenchymal stem cells, such as attachment, spreading, proliferation and differentiation, were obviously enhanced by the covalently immobilized Col/HA PEM on TCs compared with the absorbed Col/HA PEM. The improved stability and biological properties of the Col/HA PEM covalently immobilized TC may be beneficial to the early osseointegration of the implants.

scholarly journals Fabrication and Characterization of Antibacterial-active Multilayer Films Based on Keggin Polyoxometalates and Methylene Blue

2010 ◽  
Vol 65 (2) ◽  
pp. 140-146 ◽  
Author(s):  
Dan Chen ◽  
Jun Peng ◽  
Haijun Pang ◽  
Pengpeng Zhang ◽  
Yuan Chen ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Self Assembly ◽  
Multilayer Films ◽  
Photoelectron Spectra ◽  
Layer By Layer ◽  
Force Microscopy ◽  
Atomic Force ◽  
Assembly Technique ◽  
Lbl Films ◽  
Cv Measurements

Two kinds of multilayer films based on Keggin polyoxometalates α-[SiW12O40]4−/α- [PMo12O40]3− and methylene blue have been prepared via the layer-by-layer (LBL) self-assembly technique. The multilayer films were characterized by UV/Vis spectra, atomic force microscopy (AFM) and X-ray photoelectron spectra (XPS). The cyclic voltammetry (CV) measurements have demonstrated that the electrochemical properties of POMs are maintained in the LBL films. The antibacterial activity of the LBL films has also been investigated, which shows a distinct antibacterial effect against Escherichia coli.

Layer-by-layer self-assembled laminin/fucoidan films: towards better hemocompatibility and endothelialization

RSC Advances ◽  
2016 ◽  
Vol 6 (61) ◽  
pp. 56048-56055 ◽  
Author(s):  
Yan Wang ◽  
Changrong Ye ◽  
Hong Su ◽  
Juan Wang ◽  
Yanan Wang ◽  
...  
Keyword(s):  
Self Assembly ◽  
Multilayer Film ◽  
Layer By Layer ◽  
Assembly Technique ◽  
Self Assembled

The laminin/fucoidan multilayer film is prepared on glass via layer-by-layer self-assembly technique and monitored the assembled process by QCM-D. This film can inhibit platelets adhesion and improve ECs and EPCs adhesion.

Preparation and Characterization of Nano-Films Materials

2011 ◽  
Vol 492 ◽  
pp. 160-163
Author(s):  
Cai Xia Li ◽  
Qing Lv ◽  
Jie Song ◽  
Dan Yu Jiang ◽  
Qiang Li
Keyword(s):  
Self Assembly ◽  
Chemical Properties ◽  
Layered Compounds ◽  
Insertion Reaction ◽  
Layer By Layer ◽  
Vis Spectroscopy ◽  
Assembly Technique ◽  
Assembly Technology ◽  
Physical And Chemical

Nano-sheets are two-dimensional sheet materials exfoliated from the inorganic layered compounds by various physical and chemical methods. Their unique characteristics insertion reaction and excellent physical and chemical properties have attracted more and more researchers' widespread interests. Selecting quartz glass as the substrate, using layer by layer self-assembly technology, different nano-films materials are prepared. UV/Vis spectroscopy confirmed nano-films materials have been successfully assembled using LBL self-assembly technique. Raman spectrum are mainly used to analyze and characterize the structure of nano-films materials.

Controllable assembly of well-defined monodisperse Au nanoparticles on hierarchical ZnO microspheres for enhanced visible-light-driven photocatalytic and antibacterial activity

Nanoscale ◽  
2015 ◽  
Vol 7 (45) ◽  
pp. 19118-19128 ◽  
Author(s):  
Yuan Wang ◽  
Hua-Bin Fang ◽  
Yan-Zhen Zheng ◽  
Rongqin Ye ◽  
Xia Tao ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Visible Light ◽  
Self Assembly ◽  
Au Nanoparticles ◽  
Layer By Layer ◽  
Visible Light Driven ◽  
Assembly Technique

An excellent visible-light-driven photocatalyst composed of well-defined monodisperse Au nanoparticles on ZnO microspheres via a layer-by-layer self-assembly technique is demonstrated.

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